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OfflineLeon76
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Registered: 05/18/13
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Another LC question
    #18413506 - 06/13/13 12:57 PM (11 years, 7 months ago)

I made an LC from a grain LC syringe that I made thick as milk. I inoculated my jar with 10 cc and it has been on the stir plate for about 5 days now. I took it off today to check it and it is thick as hell but I noticed, in the time I let it sit, some stuff was sticking to the jar and there are a couple clumps of stuff floating in the goop as well as the top of the surface. It looks like thick myc sticking together. Is this stuff okay and is this because I put way to much to inoculate in the jar. Super, super thick syringe. Will this be okay or can it be made to thick?


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe

Edited by Leon76 (06/13/13 02:08 PM)

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OfflinecronicrMFacebook
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Re: Another LC question [Re: Leon76]
    #18413521 - 06/13/13 01:00 PM (11 years, 7 months ago)

only one way to find out, if the mycelium is at the top of the water it's because you stopped stirring and it needs to exchange gasses which can't be done in standing water so that might be a good thing as alot of common contams in lc's don't need to and won't float to the top


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisible36fuckin5
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Re: Another LC question [Re: cronicr]
    #18413778 - 06/13/13 02:02 PM (11 years, 7 months ago)

It looks fine to me, but the thing about LC is that you can't ever visually say that it's good. You have to do a test jar.


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Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflineLeon76
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Re: Another LC question [Re: 36fuckin5]
    #18415740 - 06/13/13 08:00 PM (11 years, 7 months ago)

I thought the point of a grain LC is that you can see that there are no contams. Is this not true?


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe

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OfflinecronicrMFacebook
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Re: Another LC question [Re: Leon76]
    #18415747 - 06/13/13 08:03 PM (11 years, 7 months ago)

no not really, and you didn't help by adding it to a lc.
y even make a lc from a  grain lc in the first place:shrug:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineAmanita virosa
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Re: Another LC question [Re: Leon76]
    #18415753 - 06/13/13 08:04 PM (11 years, 7 months ago)

You cannot see bacteria. But rather than testing a jar,  just drip a few drops into a sterile agar plate and grow it out. Contam will be obvious. Do this before you shoot it into grain.

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OfflineLeon76
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Re: Another LC question [Re: Amanita virosa]
    #18416510 - 06/13/13 10:43 PM (11 years, 7 months ago)

I did it just to get it down. Halfway new so just experimenting a little. Tonight when I took it off the stir plate again (I like to let the plate rest every so often) it is completely full of myc. I mean there is no water solution separation what so ever. It is completely to the top. I cannot see my stir bar at all. Is this a problem? How much will it continue to grow?


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe

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InvisibleOeric McKenna
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Re: Another LC question [Re: Leon76]
    #18416771 - 06/13/13 11:54 PM (11 years, 7 months ago)

You should've halted growth at about 1/3 of what it is now.
When it's that thick, you have no hope of sucking it up with a syringe.

Many use LC and cause their own failures with mishandling.
Many preach against LC because of the obvious inability to see contamination.

I do not advocate it's use but I do use it myself.
When you're certain it's clean, you can get a very high success rate because of the ease of innoculating through injection ports with a red hot needle. The other reason = like it, is because it's cheap and the expansion volume possibilities are vast. This is how = give away so many free edible syringes. If you have a clean master, you can lay out 20 clean syringes for 15 cents a piece. Ect ect.

Are you familiar with the use of these ports?
If you do get a clean culture, its best to avoid opening it at all.
The same way people spray the walls of a still air box to trap contaminant spores can work against you here. Contaminant spores land on, and cling to liquid surfaces. From there they easily, and readily multiply in the nutritious environment.

For future practices, there is no need to inoculate with 10cc of solution. The less you use, the better chance that you are injecting something clean. A single drop will do.


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OfflineLeon76
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Registered: 05/18/13
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Re: Another LC question [Re: Oeric McKenna]
    #18416817 - 06/14/13 12:11 AM (11 years, 7 months ago)

Will aspirating while on the stir plate help this? I do have self healing ports and also 16 gauge needles.


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe

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InvisibleOeric McKenna
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Re: Another LC question [Re: Leon76]
    #18416851 - 06/14/13 12:24 AM (11 years, 7 months ago)

If it's too thick you will pretty much be fucked no matter what.
I see your other thread about removing the liquid. Liquid and bits of mycelium is what you want.
Thick, snot-like gobs of mycelium won't be going in, or out of a needle , as you will soon discover.


--------------------


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InvisibleOeric McKenna
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Re: Another LC question [Re: Oeric McKenna]
    #18416874 - 06/14/13 12:30 AM (11 years, 7 months ago)

But I do see what you're saying about the vast amount of blank liquid you have there.. maybe that's not too thick yet. It'll eventually form really thick chunks. Your stir bar won't whip it up for you si its more uniform obviously. Grab the jar and whirl it around with your hands so that its all mixed. It'll mix up. Its not oil & water.


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love is everything
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Offlinetwistedty
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Re: Another LC question [Re: Oeric McKenna]
    #18417099 - 06/14/13 01:30 AM (11 years, 7 months ago)

AGAR solves all these problems and is much easier to use rather than waiting on boring LCs

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OfflinePussyFart
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Re: Another LC question [Re: twistedty]
    #18417129 - 06/14/13 01:38 AM (11 years, 7 months ago)

Quote:

twistedty said:
AGAR solves all these problems and is much easier to use rather than waiting on boring LCs



Truer words have never been spoken typed.

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InvisibleOeric McKenna
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Re: Another LC question [Re: PussyFart]
    #18417354 - 06/14/13 02:43 AM (11 years, 7 months ago)

Tad condescending really. I fail to see the relevance of the word boring


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OfflinePussyFart
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Re: Another LC question [Re: Oeric McKenna]
    #18417382 - 06/14/13 02:58 AM (11 years, 7 months ago)

Well, you have to wait for the LC to colonize, then wait for test jars to colonize, and hope that the LC is clean....this is time that could have been spent doing G2G transfers from a clean agar wedge.

That is how I look at it.

Waiting for an LC to colonize, and the whole time not being able to tell if it's clean or not, is not only boring in my mind, it's mentally strenuous and tactically moronic.

Just my opinion.

Edited by PussyFart (06/14/13 02:59 AM)

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InvisibleOeric McKenna
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Re: Another LC question [Re: PussyFart]
    #18417401 - 06/14/13 03:14 AM (11 years, 7 months ago)

I use red hot needles to pull from clean master slants and run liquid cultures just to have clean innoculant to play with & give away free. I have nearly 100% success rate at inoculation.
If I do 50 jars, I get 50 jars.  Mixed media liquid innoculant as described by Stamets.
I can add different woods and other substrates to it so the mycelium can learn and increase leap off/ run speed.   
    Just because some people screw up certain methods with lack of procedural understanding, or you personally don't use them, doesn't mean they are "moronic" my friend.
Speaking of which, if you ever need any edible cultures for free, I'm here to share.  Working on Pleurotus populinus & Ganoderma tsugae currently for the community. They all start on agar.


--------------------


spread love
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OfflinePussyFart
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Re: Another LC question [Re: Oeric McKenna]
    #18417423 - 06/14/13 03:32 AM (11 years, 7 months ago)

In my opinion, going from a master slant to an LC is moronic(maybe this is the wrong term)...not calling you a moron, but you know what I mean.

I see it like this...You are making an LC to eventually knock up a bunch of grains right?

Well why not just skip the LC entirely, and just knock up grains with the agar wedge and then G2G out?

You would be saving a buttload of time.  That's just how I see it....in my mind going from agar to LC, just to eventually knock up grains anyways, is like dealing with a middle man who might screw you....just use the agar to knock up the grains and be done.

No need for syringes....just a scalpel.

It's all personal preference really, but do u see my logic?

Edited by PussyFart (06/14/13 03:33 AM)

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InvisibleOeric McKenna
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Re: Another LC question [Re: PussyFart]
    #18417550 - 06/14/13 05:38 AM (11 years, 7 months ago)

The master slant is the master. The liquid is my mule.
It doesn't take up all this time like you're saying. It takes about a week , and I have syringes all year. No big deal.

Your scenario of the middle man screwing me is not a scenario for me. That's why.
I prefer injectable innoculant.  Expanding agar to agar is tedious. That's why entry level culture companies who sell cultures charge upwards of 60$ for a plate and 20$ for a culture syringe. Easily expanded in only 1 generation. I told you my price, free.
My success rate with injections is higher. I also add different mediums to the liquid mediums depending on species. The middle man isn't a middleman. 
Its just personal preference too. Like I said, I don't advovate it but I do it.  Injections are easier for me than fiddling around opening jar lids.

I realize everyone thinks the way you do and in agreement. I'm cool with that.
I think a different way.  I'll send you samples anytime. Run them or put em under your microscope. Its as clean as anything you'd buy from a culture vendor.
Out-grow, sporeworks ect. are doing the same thing I'm doing.


--------------------


spread love
love is everything
2013 finds
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OfflineLeon76
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Registered: 05/18/13
Posts: 78
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Re: Another LC question [Re: Oeric McKenna]
    #18419128 - 06/14/13 02:28 PM (11 years, 7 months ago)

That sums up pretty much how I see it as well. It may be tedious and boring, but it's a ton easier and I always have the option of going to agar. I shot up four jars three days ago and they are already about 15% colonized, that's pretty darn quick. And I can inoculate in the open, wipe the port with alcohol, flame the syringe and inject. One minute tops. You can do twelve jars in ten minutes. Try that with agar. They each have their benefits, but the hatred for the LC is misplaced, IMO.


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“I have absolutely no pleasure in the stimulants in which I sometimes so madly indulge. It has not been in the pursuit of pleasure that I have periled life and reputation and reason. It has been the desperate attempt to escape from torturing memories, from a sense of insupportable loneliness and a dread of some strange impending doom.”
― Edgar Allan Poe

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OfflinePussyFart
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Re: Another LC question [Re: Leon76]
    #18419164 - 06/14/13 02:40 PM (11 years, 7 months ago)

It's not a hatred, for me at least, it's a preference.

I prefer not to mess with syringes, and just pour grains from one jar to another.

I prefer not to have that slight chance of the LC going bad, or not being able to tell if it's clean or not.

With agar you know without a doubt most of the time that the inoculant is clean.

And FYI, I can do 12 G2G transfers in about 10 minutes.... :tongue:

Edited by PussyFart (06/14/13 02:40 PM)

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