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un-known-ome
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Registered: 03/04/12
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How Do LC's Work?
#18415433 - 06/13/13 06:59 PM (11 years, 7 months ago) |
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I have a pretty simple question, I think: if I take a multispore syringe of any arbitrary cubensis strain, and I make an LC out of it, and then I inoculate a jar of rye, and then that jar colonizes well, does that mean I can just knock-up a bunch of other jars, spawn to bulk, fruit, and then expect good results?
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cronicr



Registered: 08/07/11
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i wouldn't say good results, you would still be starting from multi-spore which is never the best route for any lc because it was likely fruited in open air. but if you get it to work your results would still be a multi spore guestimate
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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WolfieNuke
Professional Psychonaut



Registered: 12/21/09
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Re: How Do LC's Work? [Re: cronicr]
#18415912 - 06/13/13 08:37 PM (11 years, 7 months ago) |
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I guess the only thing you would be doing is allowing the spores to germinate in the LC and increasing the amount of fluid you can use to inoculate and maximize how many jars you can inoculate with a spore syringe. The results wouldn't be any different than using a spore syringe, minus the germination time (3-5 days).
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PocketRevolution
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So, assuming you've successfully colonized dozens and dozens of jars, and also had a handful of jars contaminate, you stand a chance of being able to determine whether or not your test jar is successfully colonized with nothing but your desired mycelium. If on the other hand, you're not that familiar with what a contaminated versus clean grain jar looks like, you're going to need to spawn and fruit your test jar before you can be sure it was successful. That's going to take a while. What's your LC going to be doing in the meantime? Are you prepared to wait a month or more in order to test your LC before deploying it?
I know agar work seems intimidating, especially because a lot of people focus on isolating strains. However, learning to use agar to ensure a clean sample, and/or transfer away from any contaminants that show up, is dead easy. You can do it in a still air box using widemouth half-pints as petri dishes. Then you can use a wedge to inoculate a master grain jar, then G2G that jar to 6 more, then those 6 to 36 more, until the sheer mathematics of the thing results in you not having anywhere to sit down because your entire house is full of jars full of mycelium.
Grain to grain transfers are easy to do, and have a very low contamination rate. They also don't require any syringes, or injection ports, or magnetic stir bars. Just jars and filter lids.
Don't believe the hype.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Loc: Orbiting Earth
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Quote:
PocketRevolution said: Then you can use a wedge to inoculate a master grain jar, then G2G that jar to 6 10 more, then those 6 10 to 36 100 more, and so on...
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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twistedty
Forcefully Retired



Registered: 07/01/12
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Quote:
Notahacker420 said:
Quote:
PocketRevolution said: Then you can use a wedge to inoculate a master grain jar, then G2G that jar to 6 10 more, then those 6 10 to 36 100 more, and so on...


ive read that RR says he regrets informing people of LCs
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invitro


Registered: 05/03/13
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un-known-ome
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Re: Not that well [Re: invitro]
#18418473 - 06/14/13 11:35 AM (11 years, 7 months ago) |
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Well I was meaning that now I have a colonized jar of rye from an LC. It is white and fluffy. It looks great. Can I use the same LC to make more jars, and should they fruit just fine? Alternatively, can I use the colonized jar to do G2G? In other words, if I make an LC and it colonizes a jar, does that automatically mean that it's a good jar that will spawn, colonize, and fruit?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Quote:
twistedty said:
Quote:
Notahacker420 said:
Quote:
PocketRevolution said: Then you can use a wedge to inoculate a master grain jar, then G2G that jar to 6 10 more, then those 6 10 to 36 100 more, and so on...


ive read that RR says he regrets informing people of LCs
GLCs.
RR never used LCs.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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Thadeous
On the path

Registered: 08/02/11
Posts: 1,101
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Because it is MS you cannot guarantee much.. it is a crapshoot every time. Also, every time you plunge a needle into that LC you create a vector for contamination, so you cannot even ensure it stays clean. That said, I've used them with decent success.
I am hopefully stopping from doing many more MS grows by cloning a good cluster on agar and making a LC out of that, or just doing the G2G like our friends above mentioned.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Not that well [Re: Thadeous]
#18418538 - 06/14/13 11:51 AM (11 years, 7 months ago) |
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Oh, and no spore print/syringe is 100% clean. That's another reason we don't recommend inoculating LCs with MS.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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un-known-ome
Stranger

Registered: 03/04/12
Posts: 463
Last seen: 10 years, 6 months
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Quote:
PocketRevolution said: So, assuming you've successfully colonized dozens and dozens of jars, and also had a handful of jars contaminate, you stand a chance of being able to determine whether or not your test jar is successfully colonized with nothing but your desired mycelium. If on the other hand, you're not that familiar with what a contaminated versus clean grain jar looks like, you're going to need to spawn and fruit your test jar before you can be sure it was successful. That's going to take a while. What's your LC going to be doing in the meantime? Are you prepared to wait a month or more in order to test your LC before deploying it?
I know agar work seems intimidating, especially because a lot of people focus on isolating strains. However, learning to use agar to ensure a clean sample, and/or transfer away from any contaminants that show up, is dead easy. You can do it in a still air box using widemouth half-pints as petri dishes. Then you can use a wedge to inoculate a master grain jar, then G2G that jar to 6 more, then those 6 to 36 more, until the sheer mathematics of the thing results in you not having anywhere to sit down because your entire house is full of jars full of mycelium.
Grain to grain transfers are easy to do, and have a very low contamination rate. They also don't require any syringes, or injection ports, or magnetic stir bars. Just jars and filter lids.
Don't believe the hype.
Right. I have a colonized rye jar from an LC. I haven't fruited it, but could I hypothetically do G2G with this jar as the master and have viable spawn?
Edited by un-known-ome (06/14/13 06:02 PM)
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WolfieNuke
Professional Psychonaut



Registered: 12/21/09
Posts: 421
Loc: Washington, US
Last seen: 7 years, 6 months
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I would suggest you fruit first and then isolate or else all you will be doing is using a multispore as a master, and you will most likely have inconsistent flushes from that.
Just because it colonizes doesn't mean that it will fruit successfully. Even if it does, which I hope it will, you will not have the amazing flushes that you see some of the users here have done. Most of those have isolated a successfully fruit, by cloning, and made masters from that.
Edited by WolfieNuke (06/14/13 06:31 PM)
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samael
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Registered: 08/30/12
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you schould read roger rabbits sayings.-
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