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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 5 years, 2 months
rhizomorphic growth not as visible after shake?
    #18414521 - 06/13/13 04:22 PM (11 years, 7 months ago)

so, ive had my first, and obviously my worst run ins with contams over the past couple months.  it was pretty bad though where sometimes i lost the lot of a whole strain.  im pretty certain it stemmed from me reusing old syringe needles.  i pretty much started to weed out the contams, and isolated all my clean spawn to where im not having as many problems so im fairly certain its not my clean area. 
  However, im also not out of the woods yet.  i still have a couple strains that are suspect.  i think i lost my PE as i opened one half gallon to spawn the other day and it although looked decent smelled horrible.  upon furthur inspection of the rest of the jars after oepning that one i noticed close up it doesnt look so much like myc.  not in front of it right now but ill try to get a pic.  so im pretty sure i lost that.  My guess is, while i thought i had a clean jar out of so many contams, just because it was white, i didnt smell it before g2g'ing and proly just g2g'd a contamed jar. 
  Ive since noticed the same with my AA+, but ive yet to open it to check.
  On that note, when i initially start seeing colonization from g2g jars, i can clearly see rhyzomorphs.  after shake, everything tends to take on a more even cottony look, even when not contamed.  so, its safe to say that after a shake you proly would be seeing that rhyzomorphic pattern like you would in a preshaken jar at innoc points.  or an agar plate?  just wondering cause its just something im starting to notice as i go as i never looked at certain things before or gave much thought as i didnt have contams with my first run.  only started my second go with grains this april again from teh same syringes i did from my first run back in september.
  anyhoo, i gather i will certainly be giving all my jars a sniff from here on in.  i never did before as i never had problems, and also considered it just a lil more contam risk even in front of flowhood.  im a lil more relaxed in front of my hood now.  hopefully i can put an end to this run of contams. 
  got to look up how to reuse needles now.  just never really thought about it.  can i just drop on in alcohol before putting onto the syringe if its not a fresh, sealed needle?


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!

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Invisible8ow8
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Registered: 05/22/13
Posts: 196
Loc: Canada
Re: rhizomorphic growth not as visible after shake? [Re: DeadPhan]
    #18414543 - 06/13/13 04:26 PM (11 years, 7 months ago)

method 1:

Pop some used needles into an empty jar an PC them along with the rest of your grain. If you use a complete regular canning lid, it will self-seal and remain more or less sterile until you're ready to use it.

method 2:

Just buy some sterile needles. they are cheap. You can get them anywhere.


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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 5 years, 2 months
Re: rhizomorphic growth not as visible after shake? [Re: 8ow8]
    #18414558 - 06/13/13 04:30 PM (11 years, 7 months ago)

method one it is.  :facepalm:  oh the foils i could of avoided if i had thought of that.  i capped and threw them into a plastic bag in front of the flow hood and thought i was good.  doh!


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
Trusted Cultivator
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Re: rhizomorphic growth not as visible after shake? [Re: DeadPhan]
    #18414601 - 06/13/13 04:41 PM (11 years, 7 months ago)

Quote:

DeadPhan said:
method one it is.  :facepalm:  oh the foils i could of avoided if i had thought of that.  i capped and threw them into a plastic bag in front of the flow hood and thought i was good.  doh!




Yeah, that's definitely your problem.

I hardly ever PC mine though. I just fill it with alcohol while I boil some water. Once it's at a good, rolling boil, I expel the alcohol into the sink, then fill it 3 times with boiling water. On the last time, leave the syringe full of water. Once it's back to room temp, it's good to go.

If you're gonna PC them, I suggest you wrap them in foil. That way you can use one and not contam the rest.


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Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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