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Invisibletko
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Registered: 05/08/13
Posts: 496
couple brf agar ?'s
    #18412916 - 06/13/13 10:27 AM (11 years, 7 months ago)

ok so i did the substitute and took a clone,,,blah blah. but iwas wondering a few things. one, does the bottom have to be colonized 100% the top half has been done for a week,is it possible to just scrape off the top portion like flake it off with razor? also it was spur of moment thing n used a half pint jar,will this be awkward trying to cut it? thanks for any input dudes. or girls..lol

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Invisible36fuckin5
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Registered: 08/11/03
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Re: couple brf agar ?'s [Re: tko]
    #18412946 - 06/13/13 10:37 AM (11 years, 7 months ago)

It's only supposed to be colonized on top. And you're not supposed to let it run to the edge. I'd start over.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Invisibletko
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Registered: 05/08/13
Posts: 496
Re: couple brf agar ?'s [Re: 36fuckin5]
    #18413083 - 06/13/13 11:09 AM (11 years, 7 months ago)

can you elaborate a little??

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OfflinePrinceShroom
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Re: couple brf agar ?'s [Re: tko]
    #18413101 - 06/13/13 11:13 AM (11 years, 7 months ago)

leaving it run to the edge isnt bad IME.  If your trying to isolate then its a different story you want to cut a transfer from the leading edge of the growing mycellium.  You can use a colonized plate fine.  I personally stay away from the 1/4 inch around the outside of the plate cause this is more likely where contames will fall and stay.

You just cut a wedge and use it to inoc.  The mycellium will completely cover the agar once its been cut.  Mycellium on agar always grows in 2d.


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.

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Invisible36fuckin5
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Re: couple brf agar ?'s [Re: PrinceShroom]
    #18413258 - 06/13/13 12:07 PM (11 years, 7 months ago)

Quote:

PrinceShroom said:
leaving it run to the edge isnt bad IME. I personally stay away from the 1/4 inch around the outside of the plate cause this is more likely where contames will fall and stay.




So... you're saying it is bad then. Edge contamination is the problem, which is what you're talking about.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Invisibletko
Stranger

Registered: 05/08/13
Posts: 496
Re: couple brf agar ?'s [Re: 36fuckin5]
    #18413265 - 06/13/13 12:09 PM (11 years, 7 months ago)

so just cut straight down till hit glass then cut a wedge out,and the uncolonized portion doesnt cause any harm? also wanted keep culture going and the most rhizos are along the edge so maybe take a few off the edge or are you saying edges=no good? thanks bud and i think he means the plate can be used but try to avoid using the edges..

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Invisible36fuckin5
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Re: couple brf agar ?'s [Re: tko]
    #18413274 - 06/13/13 12:11 PM (11 years, 7 months ago)

Edges are no good. That should answer everything you just asked. Yes, cut to the glass, how else would you get a wedge out?


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflinePrinceShroom
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Re: couple brf agar ?'s [Re: 36fuckin5]
    #18413282 - 06/13/13 12:13 PM (11 years, 7 months ago)

Quote:

36fuckin5 said:
It's only supposed to be colonized on top. And you're not supposed to let it run to the edge. I'd start over.



Why cant you let it run to the edge?  Im saying dont use the 1/4 inch closest to the edge of the plate cause that is most likely where contam will enter and sit.  This I just do to be on the safe side ive used up full dishes before with out contam.  Im just trying to err on the side of caution.  What I dont get is why your saying that once a plate is colonized its no good and you would start over.  I have many many fully colonized plates that I reg use to make up grain masters.  Your only concerned about the leading edge if you are trying to get an iso.  If you already have one let it fill the plate and cut wedges from it to use anywhere...


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.

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OfflinecronicrMFacebook
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Re: couple brf agar ?'s [Re: PrinceShroom]
    #18413294 - 06/13/13 12:15 PM (11 years, 7 months ago)

Quote:

PrinceShroom said:
leaving it run to the edge isnt bad IME.  If your trying to isolate then its a different story you want to cut a transfer from the leading edge of the growing mycellium. 



i know this ain't where you were going with this but just to point it out, brf agar sub sucks balls for trying to isolate, and not that you would want to from a clone tissue anyway:cool:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offline10KOysters
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Registered: 04/18/13
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Re: couple brf agar ?'s [Re: tko]
    #18413301 - 06/13/13 12:17 PM (11 years, 7 months ago)

Quote:

tko said:
so just cut straight down till hit glass then cut a wedge out



Actually, if it's pure brf agar, you'll want to just scrape some of the mycelia off and transfer that.

The mycelia (at least with my brf agar) doesn't really grow into the brf agar very well at first.  Only after it colonizes the entire outer surface do you start to see real penetration of the mycelia.

It scrapes off quite easily.

Peace


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I object to the prolific use of 'Submit' buttons on the internet

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Invisible36fuckin5
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Re: couple brf agar ?'s [Re: PrinceShroom]
    #18413313 - 06/13/13 12:20 PM (11 years, 7 months ago)

Quote:

PrinceShroom said:
Why cant you let it run to the edge?




Quote:

Im saying dont use the 1/4 inch closest to the edge of the plate cause that is most likely where contam will enter and sit.




You've answered your own question twice in a row now. Spores can get onto the myc and be transferred. It's also best to use the leading edge because that's the youngest, most vigorous myc you have.

I would start over because it's cheap and easy, and I like things done just right.

Quote:

The mycelia (at least with my brf agar) doesn't really grow into the brf agar very well at first.  Only after it colonizes the entire outer surface do you start to see real penetration of the mycelia.




It's not supposed to grow into the BRF. It's supposed to be a 2-D media.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Invisibletko
Stranger

Registered: 05/08/13
Posts: 496
Re: couple brf agar ?'s [Re: 36fuckin5]
    #18413368 - 06/13/13 12:33 PM (11 years, 7 months ago)

like i said, was in a bind, i heard brf works. so i did it...all i asked was if the uncolonized portion will cause a problem,not asking for an  oh id throw it out cause all my shits PERFECT answer.. thanks

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OfflinecronicrMFacebook
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Re: couple brf agar ?'s [Re: tko]
    #18413385 - 06/13/13 12:35 PM (11 years, 7 months ago)

no the uncolonized stuff won't harm anything as long as your doing your work in a gb or sab


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisibletko
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Registered: 05/08/13
Posts: 496
Re: couple brf agar ?'s [Re: cronicr]
    #18413432 - 06/13/13 12:44 PM (11 years, 7 months ago)

alright bruh

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OfflinePrinceShroom
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Re: couple brf agar ?'s [Re: cronicr]
    #18413454 - 06/13/13 12:48 PM (11 years, 7 months ago)

Quote:

cronicr said:
Quote:

PrinceShroom said:
leaving it run to the edge isnt bad IME.  If your trying to isolate then its a different story you want to cut a transfer from the leading edge of the growing mycellium. 



i know this ain't where you were going with this but just to point it out, brf agar sub sucks balls for trying to isolate, and not that you would want to from a clone tissue anyway:cool:



BRF fert agar if made properly is the most rhizomorphic agar I have ever seen and ive poured 1000's of plates.  I may be ne to shroomery but ive been growing steady for 5 yrs.  3 of those yrs were to large scale.  Ive messed around with so many different ways to grow.  I have only found one sustainable way to grow to scale.  I find pasteurized subs arent worth it.  Having to toss out 100's of lbs of used substrate is heat score.  The logistics of it all.  Ive found you can grow about 5 lbs in one location and then you hit a wall of logistics.  To grow more you have to goto a different location and even with rooms set up dedicated to the grow 35-50 tubs is all about that can b handled at one location.  I count my success rate for mono's at about 25%  Sure I get 8-16 o's from a tub but it always trichs and alot of times ur lucky to gt 4-6 ounces even with a clone or iso.  These TC's saying they are pulling off 8 o first flushes regularly I call BS on them.  Sure anyone can have a good tub 7-9 o's first flush but its not a regular occurrence.  This is known fr alot of experience.  Not from just peeking the shrooomery.

I did say that contams are most likely to occur around the edges thats the most open place.  So even with a flowhood im still worried about contams getting in.  Better be safer than sorry.  On the other hand though I have used the edge of plates many many times and rarely see contams.


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.

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OfflinecronicrMFacebook
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Re: couple brf agar ?'s [Re: PrinceShroom]
    #18413463 - 06/13/13 12:50 PM (11 years, 7 months ago)

yes the brf with agar will be fine for isolating and is much easier to work with, but he just used flour and water:thumbup:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinecronicrMFacebook
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Re: couple brf agar ?'s [Re: PrinceShroom]
    #18413479 - 06/13/13 12:53 PM (11 years, 7 months ago)

Quote:

PrinceShroom said:
  These TC's saying they are pulling off 8 o first flushes regularly I call BS on them.  Sure anyone can have a good tub 7-9 o's first flush but its not a regular occurrence.  This is known fr alot of experience.  Not from just peeking the shrooomery.





:lmafo::laugh2::lmafo::laugh2:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinePussyFart
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Re: couple brf agar ?'s [Re: PrinceShroom]
    #18413499 - 06/13/13 12:55 PM (11 years, 7 months ago)

The things some noobs say...lol

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OfflinePrinceShroom
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Re: couple brf agar ?'s [Re: cronicr]
    #18413658 - 06/13/13 01:33 PM (11 years, 7 months ago)

Quote:

Notahacker420 said:
The things some noobs say...lol



:noob: ive grown to scale (10+ lbs a month) for 3 years.  Ive been growing for over five.  The first wk I started growing I did cakes cased rye trays, lc's and to top it off I started my first iso within a wk of starting to grow. I guarantee you that ive done more jars trays monos then you have ever seen.  I read the TMC in 83.  I have an idea of what I am doing.

IME spawning to pasteurized sub's leads to contams all the time for me.  Bucket tek, or proper pasteurization.  No matter, contams.  I have a hood all my spawn is clean my lc's my syringes.  Just whenever i spawn i run into contams.  The logistics of doing 5+lbs/month at one location just doesnt work.

Quote:

cronicr said:
Quote:

PrinceShroom said:
  These TC's saying they are pulling off 8 o first flushes regularly I call BS on them.  Sure anyone can have a good tub 7-9 o's first flush but its not a regular occurrence.  This is known fr alot of experience.  Not from just peeking the shrooomery.





:lmafo::laugh2::lmafo::laugh2:



Im good friends with many TC's on this site.  Im not going to mention names but there are TC's here claiming 8 o first flushes and really its 6 and very rare for them to get it.  Even with a clone or iso.  I know ive tried it too many times.  Pound for pound spawned subs dont work out in our benefit. a mono weighs 10-15 lbs and you end up with a half pound dry.  Whats the BE on that? Not vry good.


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.

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OfflinePrinceShroom
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Re: couple brf agar ?'s [Re: PrinceShroom]
    #18413685 - 06/13/13 01:39 PM (11 years, 7 months ago)

Thats like 10-15% BE thats ridiculous. Ask a professional button farmer what his BE is.  Ill tell you usually around 150-200% Yet people are happy with 10-15:smirk:

There is a new better way to grow coming up.  Its unbelievable.  ANy noob with a little agar skills and a pc can do it easy as pie.  And if a contam enters your only losing a small portion of your crop not your whole tub.


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.

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