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Offlinenerveagent
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Registered: 06/13/03
Posts: 243
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WBS ------> CORN ??
    #1841162 - 08/22/03 05:16 PM (13 years, 3 months ago)

Out of 6 jars one is about 20% colonized and the others look like they're waterlogged. My question is could I remove the colonized part from the WBS and then transfer the mycelium into newly prepared jars of corn? Would there be any problems with this?


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OfflineMsPacMan
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Re: WBS ------> CORN ?? [Re: nerveagent]
    #1841170 - 08/22/03 05:18 PM (13 years, 3 months ago)

how long since you innoculated?


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Offlinewhole9
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Registered: 04/28/03
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Re: WBS ------> CORN ?? [Re: MsPacMan]
    #1841218 - 08/22/03 05:35 PM (13 years, 3 months ago)

you CAN do it, but there would be a huge risk of contams even you are some what sterile. I wouldnt recomend it, but you CAN do it.


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InvisibleATWAR
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Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: WBS ------> CORN ?? [Re: MsPacMan]
    #1841228 - 08/22/03 05:38 PM (13 years, 3 months ago)

Yes, you could. But, unless you have a flow hood or a glove box, then the risk of contamination will be very high. If you must do it open air, then try to be as clean as possible. Spray the air with sanitizer, and be very quick. I give you about a 10% chance of success.


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Offlinenerveagent
Come on Pilgrim

Registered: 06/13/03
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Re: WBS ------> CORN ?? [Re: ATWAR]
    #1842426 - 08/23/03 12:23 AM (13 years, 3 months ago)

It's been about 3 weeks since innoculation.


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InvisibleSubGen1us
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Registered: 11/26/02
Posts: 3,427
Re: WBS ------> CORN ?? [Re: nerveagent]
    #1842470 - 08/23/03 12:45 AM (13 years, 3 months ago)

wait till the the 1 jar is 100% colonized, then g2g transfer. watch ur water content carfully cause that will make u or break u. excess water is the leading cause of bacterial contamination.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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