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psiconautasmart
Stranger

Registered: 02/05/13
Posts: 36
Last seen: 6 years, 3 months
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What's going on with my agar cultures?
#18409613 - 06/12/13 05:08 PM (11 years, 7 months ago) |
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Hello mycophiles,
I recently inoculated some fresh petris with fragments of rhizomorphic mycelium from a P1, first generation (spores), petri dish. It seemed like a very healthy sector. All petris were inoculated from the same P1 dish.
Does anyone know why the first petris show a somewhat darker growth spot in the center changing to a whiter, more rhizomorphic growth on the edges? Could it be that the mycelium was still recuperating from the shock and then got healthier from a certain point on?
In the 1st and 4th pictures a triangular spot of almost no growth appears, why does this happen, anyone knows? Maybe there is some whitish mold I haven't distinguished...
On pictures 5, 6 & 7 a cottony growth appears. I'm not sure if this looks like cubensis mycelium. On picture 7, 2 types of growth appear. Healthy rhyzomorphic, and this cottony type. Could it be a kind of white mold, or is it a differentiated sector of cubensis mycelium? I will greatly appreciate your help people. Thanks.
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ganjfather
uncle randy



Registered: 08/06/09
Posts: 6,342
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I'm no expert but it looks like you are growing some green mold in those.
I'd toss em.
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Trippy_Penguin



Registered: 03/18/12
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Re: What's going on with my agar cultures? [Re: ganjfather]
#18409873 - 06/12/13 06:09 PM (11 years, 7 months ago) |
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Re: What's going on with my agar cultures? [Re: Trippy_Penguin] 1
#18409904 - 06/12/13 06:16 PM (11 years, 7 months ago) |
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You don't toss petri dishes when contamination shows up. You transfer clean mycelium to a fresh dish, and then repeat the process until you have a pure culture.
The darker color in the middle is normal. It's also normal for mycelium to change from cottony to rhizomorphic and back. RR
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
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ganjfather
uncle randy



Registered: 08/06/09
Posts: 6,342
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Re: What's going on with my agar cultures? [Re: RogerRabbit]
#18410107 - 06/12/13 06:53 PM (11 years, 7 months ago) |
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That dark green in the middle is normal??
I'll take your word for it because I've never tried agar, that just surprises me.
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Trippy_Penguin



Registered: 03/18/12
Posts: 624
Last seen: 4 years, 4 months
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Re: What's going on with my agar cultures? [Re: ganjfather]
#18410153 - 06/12/13 07:01 PM (11 years, 7 months ago) |
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Correct me if I'm wrong, but isn't that dish beyond saving or would you actually attempt to cut out some of that rhizo?
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psiconautasmart
Stranger

Registered: 02/05/13
Posts: 36
Last seen: 6 years, 3 months
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Re: What's going on with my agar cultures? [Re: RogerRabbit]
#18413642 - 06/13/13 01:27 PM (11 years, 7 months ago) |
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RogerRabbit,
You again with a satisfying reply, thanks a lot. I really didn't think they were contaminated with mold because I have encountered molds that are much greener, but I was somewhat concerned. I trusted the fact that normally molds outgrow cubensis mycelium in no time and inhibit its growth, and that my plates show rhizomorphic growth up to the edges; but with your response now I am very confident that my plates are mostly clean of contaminants.
Good to know that it can become cottony back from rhizomorphic. Thanks everyone for your replies.
Good vibes & cultures for everyone!
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
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Re: What's going on with my agar cultures? [Re: Trippy_Penguin]
#18413667 - 06/13/13 01:35 PM (11 years, 7 months ago) |
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Quote:
petersodm said: Correct me if I'm wrong, but isn't that dish beyond saving or would you actually attempt to cut out some of that rhizo?
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10KOysters
Food Cultivator
Registered: 04/18/13
Posts: 429
Last seen: 11 years, 6 months
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Re: What's going on with my agar cultures? [Re: ConcreteWaves]
#18414419 - 06/13/13 04:06 PM (11 years, 7 months ago) |
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If I really had to do a transfer, it would be from the third pic.
The rest look like way too much work.
Peace
-------------------- I object to the prolific use of 'Submit' buttons on the internet
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