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pfshroomer
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ps. ovioids liquid culture
#18405777 - 06/11/13 10:19 PM (11 years, 7 months ago) |
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Im trying to make some LC with a ps. ovioids print... i used maple syrup... Think this might work?? It's basically just a test run.. I'm so used to growing cubies thats y i used the maple syrup..plus I know this forum has evolved so much since back when i used to grow cubensis... Anyways I just wanted to mess around a little bit and see what you guys had to say
Thanks~ PF
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18405786 - 06/11/13 10:20 PM (11 years, 7 months ago) |
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Sounds like a recipe for disaster. Maple syrup isn't normally used for LC, plus your print is likely from a wild find, meaning it's probably loaded with contams.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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cronicr



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Re: ps. ovioids liquid culture [Re: 36fuckin5]
#18405805 - 06/11/13 10:24 PM (11 years, 7 months ago) |
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agar is your best route for sure
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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pfshroomer
MASTER OF MY DOMAIN



Registered: 10/19/03
Posts: 4,952
Loc: seeking the truth
Last seen: 3 years, 11 months
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Re: ps. ovioids liquid culture [Re: 36fuckin5]
#18405807 - 06/11/13 10:24 PM (11 years, 7 months ago) |
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Yeah true... i was just sitting around with nothing to do and decided why not give it a try you know...so how should I go about doing this??
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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36fuckin5
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Registered: 08/11/03
Posts: 12,097
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18405811 - 06/11/13 10:25 PM (11 years, 7 months ago) |
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Pour some petris, swipe some spores onto it.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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pfshroomer
MASTER OF MY DOMAIN



Registered: 10/19/03
Posts: 4,952
Loc: seeking the truth
Last seen: 3 years, 11 months
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18405818 - 06/11/13 10:27 PM (11 years, 7 months ago) |
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Agar..yeah..i suppose..what would i put in the plate besides the spores of course... you guys gotta understand I know I have been a member of this website for 10 years... but I haven't grown mushrooms in over 5 years now and I know this website has come a long way since then!!!
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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36fuckin5
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Posts: 12,097
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18405829 - 06/11/13 10:28 PM (11 years, 7 months ago) |
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Search.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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pfshroomer
MASTER OF MY DOMAIN



Registered: 10/19/03
Posts: 4,952
Loc: seeking the truth
Last seen: 3 years, 11 months
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18405830 - 06/11/13 10:29 PM (11 years, 7 months ago) |
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Im gonna be honest man..all i know is growing cubes with WBS...bulk methods...i hate to say this but i have no idea what u guys mean when you say petris,etc...lol
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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pfshroomer
MASTER OF MY DOMAIN



Registered: 10/19/03
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18405833 - 06/11/13 10:30 PM (11 years, 7 months ago) |
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Ive been searching!!!
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18406049 - 06/11/13 11:11 PM (11 years, 7 months ago) |
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No LC's from that print! i can guarantee you there are contams present
get 2-3 of these at an asian/international store

get 1lb of DME at a winery/brewery store

5g's of each (1/4tbs = 1g) in 250ml (or equivalent ratios) of water
slow simmer for 10min or until fully dissolved
load into petris/jars a few MM deep
cut some tyvek lids or holes for poly if you're using jars
PC @15psi for 35min
*if there's residual moisture rolling around on the agar wait for it to evaporate, usually after a day or two* once cooled scrape a small amount of spores onto agar in a SAB. A paper clip can easily be made into a loop for scraping.
wait for clean growth 5-10 days transfer clean myc to new agar in SAB wait for more clean growth 5-10 days transfer to grain/WBS/etc in SAB colonize transfer to wood based media colonize lay beds and pray for rain
I prefer using the ball elites with tyvek lids due to their wide base and availability, but near any sealable and PC safe container will work.
If you really want a LC do a G2LC once you have a healthy, colonized jar
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/11/13 11:19 PM)
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pfshroomer
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Thanks VG
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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pfshroomer
MASTER OF MY DOMAIN



Registered: 10/19/03
Posts: 4,952
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18426638 - 06/16/13 08:33 AM (11 years, 6 months ago) |
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Haha...guys it worked...i lysold the fuck outta the room i was using,my mason jar,and the lid and knife i used to scrape off spores.. Let me figure out how to upload pictures from my cell phone and I will show you guys but there are no contams...
One time I took a chunk of mycelium from a cube grow and threw in some dirt from outside and a little bit of grass and within 5 days I had about 7 mushrooms and one was the biggest one out of the whole grow that we had going and we had about 15 small tubs so I've been doing this for a while now for about 10 years so I think I'm decently versed( is that the right word to use in this sentence)in mycology and mushroom cult. but I know You guys know a lot more tricks than I do I just gotta keep it old school
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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pfshroomer
MASTER OF MY DOMAIN



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Re: ps. ovioids liquid culture [Re: pfshroomer] 1
#18426644 - 06/16/13 08:35 AM (11 years, 6 months ago) |
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But yeah VG you are right man...ps.ovioids myceleium is fuckin strong!!! Man this takes me back to the day I haven't done this in years it feels awesome I feel like a little kid again..ive still got it
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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cronicr



Registered: 08/07/11
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18426783 - 06/16/13 09:26 AM (11 years, 6 months ago) |
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great to hear!
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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VirginiaGentleman
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18427774 - 06/16/13 01:35 PM (11 years, 6 months ago) |
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Quote:
pfshroomer said: Haha...guys it worked...i lysold the fuck outta the room i was using,my mason jar,and the lid and knife i used to scrape off spores.. Let me figure out how to upload pictures from my cell phone and I will show you guys but there are no contams...
But yeah VG you are right man...ps.ovioids myceleium is fuckin strong!!!
Good to hear, i've heard claims that it's possible to inoc. BRF jars with syringes from wild ovoids, and due to their aggressive and fast growth they outpace contams.
Still.. it would be wise to get some on agar and play it safe. If you don't have a PC, i imagine steam sterilization could also work.
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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pfshroomer
MASTER OF MY DOMAIN



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Right... Im wondering whats best to use when my LC is ready to use...ya know with cubes you use WBS...well with these ovioids should i just grab some old decaying wood(ill try n get the wood they prefer)and then throw that in a jar...PC it,then inoculate!!?? Thanks guys as this is my first wood loving grow
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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MacMerdin
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18433579 - 06/17/13 07:39 PM (11 years, 6 months ago) |
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Quote:
pfshroomer said: well with these ovioids should i just grab some old decaying wood(ill try n get the wood they prefer)and then throw that in a jar...PC it,then inoculate!!??
Grab the wood and soak it for a day. Drain it well after. Mix in moisturized (field capacity) vermiculite with the wood to give the mycelium something to bridge the gaps and also hold moisture. Just make sure the final result isn't too wet. PC it and shoot it up.
Once colonized, plant the cake somewhere it will enjoy and grow.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: ps. ovioids liquid culture [Re: MacMerdin]
#18436046 - 06/18/13 09:58 AM (11 years, 6 months ago) |
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Quote:
MacMerdin said:
Quote:
pfshroomer said: well with these ovioids should i just grab some old decaying wood(ill try n get the wood they prefer)and then throw that in a jar...PC it,then inoculate!!??
Grab the wood and soak it for a day. Drain it well after. Mix in moisturized (field capacity) vermiculite with the wood to give the mycelium something to bridge the gaps and also hold moisture. Just make sure the final result isn't too wet. PC it and shoot it up.
Once colonized, plant the cake somewhere it will enjoy and grow.
No, don't do this at all. One, it's not likely to take off well, and if it does, it's gonna take FOREVER. And when you spawn, that'll also take fucking forever because you don't have many inoculation points.
OP, either do up a bag of sawdust spawn or just go to some kind of grain. Then spawn either of those to woodchips after they colonize.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,068
Loc: The Island of Misfit Toys
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Re: ps. ovioids liquid culture [Re: 36fuckin5]
#18436083 - 06/18/13 10:06 AM (11 years, 6 months ago) |
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Well, I don't do liquid cultures but I DO do pf jars directly from spores for woodlovers with wood added.
Please look at my gallery under growing woodlovers and tell me this is not a viable method. And no.....it doesn't take fucking forever.
Edited by MacMerdin (06/18/13 10:16 AM)
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36fuckin5
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Registered: 08/11/03
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Re: ps. ovioids liquid culture [Re: MacMerdin]
#18436126 - 06/18/13 10:19 AM (11 years, 6 months ago) |
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Quote:
MacMerdin said: Please look at my gallery under growing woodlovers and tell me this is not a viable method. And no.....it doesn't take fucking forever.
I didn't say it wouldn't work, I said there are better methods.
And yes, it does take fucking forever compared to using grain. I've tried both.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: ps. ovioids liquid culture [Re: MacMerdin]
#18436362 - 06/18/13 11:15 AM (11 years, 6 months ago) |
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Quote:
MacMerdin said: Well, I don't do liquid cultures but I DO do pf jars directly from spores for woodlovers with wood added.
Please look at my gallery under growing woodlovers and tell me this is not a viable method. And no.....it doesn't take fucking forever.
so whats the plan after the ovoid cakes are colonized? are you going to put them in wood? try fruiting from cake? etc, etc,
from what i've seen 300mL of grain takes about 10-12 days to colonize from agar cuttings, what sort of time have the cakes been taking?
I might have to give your method a shot
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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MacMerdin
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Quote:
VirginiaGentleman said: so whats the plan after the ovoid cakes are colonized? are you going to put them in wood? try fruiting from cake? etc, etc,
I crumble them and spawn to more wood just like you would with grains.
Quote:
from what i've seen 300mL of grain takes about 10-12 days to colonize from agar cuttings, what sort of time have the cakes been taking?
The cakes take about 2-3 weeks. So, when you factor in the time it took you to get to a clean agar wedge, this may or may not be faster. But, it has the added benefit of already having wood for the mycelium to enjoy. Which means no lag time from them having to figure out how to start digesting wood.
Quote:
I might have to give your method a shot 
To each his own. I'm not trying to preach a certain method over another.
I was just answering the OP's question of what he could do with the wood and liquid culture he already had.
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pfshroomer
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Re: ps. ovioids liquid culture [Re: MacMerdin]
#18444928 - 06/19/13 10:34 PM (11 years, 6 months ago) |
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Ok..thats whats up thanks guys..
Ps-so ovioids WILL grow on grain..say wild bird seed?
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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cronicr



Registered: 08/07/11
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18445124 - 06/19/13 11:18 PM (11 years, 6 months ago) |
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yes they will colonize grain
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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psylosymonreturns
aka Gym Sporrison


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Re: ps. ovioids liquid culture [Re: cronicr]
#18446092 - 06/20/13 08:29 AM (11 years, 6 months ago) |
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Ovoids will grow on anything. They are very vigorous!
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pfshroomer
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okay...thank you!
So after my LC is done,I can inoculate some Jars with WBS and then spawn that onto a bed of perlite,woodchips,and hard wood..??
Thanks guys!!
I really appreciate your guys' help as this is my FIRST woodloving grow!!
Thanks again!! Much Love
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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cronicr



Registered: 08/07/11
Posts: 62,899
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18449115 - 06/20/13 08:16 PM (11 years, 6 months ago) |
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skip the perlite
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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psylosymonreturns
aka Gym Sporrison


Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
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Re: ps. ovioids liquid culture [Re: cronicr]
#18449177 - 06/20/13 08:27 PM (11 years, 6 months ago) |
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Id save the perlite as well , not needed at all in this situation.
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VirginiaGentleman
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just an idea, why not mix some soaked wood chips with grains? Like Mac's+BRF but with WBS+wood chips
may use that idea for an experimental Allenii jar on RBS
or it could be a terrible idea and throw off the jars 
but they do seem to slow from Grain/Spawn2Wood method..
Edited by VirginiaGentleman (06/20/13 09:49 PM)
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pfshroomer
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I dont know man.. This LC is TAKING OFF!! I mean it is literally growing fast as fuck!!
So I dont see why it wouldnt grow just as fast when I spawned some WBS to Wood/woodchips,ya know...
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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VirginiaGentleman
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Re: ps. ovioids liquid culture [Re: pfshroomer]
#18449875 - 06/20/13 11:00 PM (11 years, 6 months ago) |
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well i'm interested to see what happens with a wild print/LC/spawn. seems risky, with high reward... i like it
ovoid speed &
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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pfshroomer
MASTER OF MY DOMAIN



Registered: 10/19/03
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Well so far the wild print and the LC is working man... I just lysol'd everything REALLY good and lysol'd the air I was working in..and Ive got a lot of Mycelium growing already..and it just keeps getting bigger and bigger every time I look at it.. Ill go about 3 days without checking it and it will have literally quadrupled in size(the mycelium)in 3 days..You were right about this species growing fast..or colonizing fast..same thing.. Im a lil buzzed 
Anyways.. I really appreciate all your guys' help with this..
Im wondering if a Ps. Cyanescens grow tek would work for this species(Ps.Oviods)...I didnt know since Oviods grow in hot,warmer climates and cyans grow in the cold ya know.. Anyways..Thanks again for all the help!!
I will post pics ASAP.. Ive just been busy... Ill try and do that tomorrow!!!
Peace
-------------------- "Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."
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