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Offlinepfshroomer
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ps. ovioids liquid culture
    #18405777 - 06/11/13 10:19 PM (11 years, 7 months ago)

Im trying to make some LC with a ps. ovioids print... i used maple syrup...
Think this might work?? It's basically just a test run..
I'm so used to growing cubies thats y i used the maple syrup..plus I know this forum has evolved so much since back when i used to grow cubensis... Anyways I just wanted to mess around a little bit and see what you guys had to say

Thanks~
PF


--------------------
"Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."

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Invisible36fuckin5
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18405786 - 06/11/13 10:20 PM (11 years, 7 months ago)

Sounds like a recipe for disaster. Maple syrup isn't normally used for LC, plus your print is likely from a wild find, meaning it's probably loaded with contams.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflinecronicrMFacebook
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Re: ps. ovioids liquid culture [Re: 36fuckin5]
    #18405805 - 06/11/13 10:24 PM (11 years, 7 months ago)

agar is your best route for sure


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinepfshroomer
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Re: ps. ovioids liquid culture [Re: 36fuckin5]
    #18405807 - 06/11/13 10:24 PM (11 years, 7 months ago)

Yeah true... i was just sitting around with nothing to do and decided why not give it a try you know...so how should I go about doing this??


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"Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."

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Invisible36fuckin5
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18405811 - 06/11/13 10:25 PM (11 years, 7 months ago)

Pour some petris, swipe some spores onto it.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Offlinepfshroomer
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18405818 - 06/11/13 10:27 PM (11 years, 7 months ago)

Agar..yeah..i suppose..what would i put in the plate besides the spores of course... you guys gotta understand I know I have been a member of this website for 10 years... but I haven't grown mushrooms in over 5 years now and I know this website has come a long way since then!!!


--------------------
"Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."

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Invisible36fuckin5
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18405829 - 06/11/13 10:28 PM (11 years, 7 months ago)

Search.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
Offlinepfshroomer
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Registered: 10/19/03
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18405830 - 06/11/13 10:29 PM (11 years, 7 months ago)

Im gonna be honest man..all i know is growing cubes with WBS...bulk methods...i hate to say this but i have no idea what u guys mean when you say petris,etc...lol


--------------------
"Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."

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Offlinepfshroomer
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18405833 - 06/11/13 10:30 PM (11 years, 7 months ago)

Ive been searching!!! :smile:


--------------------
"Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."

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InvisibleVirginiaGentleman
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18406049 - 06/11/13 11:11 PM (11 years, 7 months ago)

No LC's from that print! i can guarantee you there are contams present

get 2-3 of these at an asian/international store


get 1lb of DME at a winery/brewery store


5g's of each (1/4tbs = 1g) in 250ml (or equivalent ratios) of water

slow simmer for 10min or until fully dissolved

load into petris/jars a few MM deep

cut some tyvek lids or holes for poly if you're using jars

PC @15psi for 35min

*if there's residual moisture rolling around on the agar wait for it to evaporate, usually after a day or two*
once cooled scrape a small amount of spores onto agar in a SAB. A paper clip can easily be made into a loop for scraping.

wait for clean growth 5-10 days
transfer clean myc to new agar in SAB
wait for more clean growth 5-10 days
transfer to grain/WBS/etc in SAB
colonize
transfer to wood based media
colonize
lay beds and pray for rain

I prefer using the ball elites with tyvek lids due to their wide base and availability, but near any sealable and PC safe container will work.

If you really want a LC do a G2LC once you have a healthy, colonized jar


--------------------
Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only

Edited by VirginiaGentleman (06/11/13 11:19 PM)

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Offlinepfshroomer
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Re: ps. ovioids liquid culture [Re: VirginiaGentleman]
    #18406130 - 06/11/13 11:31 PM (11 years, 7 months ago)

Thanks VG


--------------------
"Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."

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Offlinepfshroomer
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18426638 - 06/16/13 08:33 AM (11 years, 6 months ago)

Haha...guys it worked...i lysold the fuck outta the room i was using,my mason jar,and the lid and knife i used to scrape off spores..
Let me figure out how to upload pictures from my cell phone and I will show you guys but there are no contams...

One time I took a chunk of mycelium from a cube grow and threw in some dirt from outside and a little bit of grass and within 5 days I had about 7 mushrooms and one was the biggest one out of the whole grow that we had going and we had about 15 small tubs so I've been doing this for a while now for about 10 years so I think I'm decently versed( is that the right word to use in this sentence)in mycology and mushroom cult. but I know You guys know a lot more tricks than I do I just gotta keep it old school


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"Follow the medicine path with an open mind and a clear heart, and you will surely find inner peace and enlightenment."

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Offlinepfshroomer
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Re: ps. ovioids liquid culture [Re: pfshroomer] * 1
    #18426644 - 06/16/13 08:35 AM (11 years, 6 months ago)

But yeah VG you are right man...ps.ovioids myceleium is fuckin strong!!! Man this takes me back to the day I haven't done this in years it feels awesome I feel like a little kid again..ive still got it :smile:


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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18426783 - 06/16/13 09:26 AM (11 years, 6 months ago)

great to hear!


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleVirginiaGentleman
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18427774 - 06/16/13 01:35 PM (11 years, 6 months ago)

Quote:

pfshroomer said:
Haha...guys it worked...i lysold the fuck outta the room i was using,my mason jar,and the lid and knife i used to scrape off spores..
Let me figure out how to upload pictures from my cell phone and I will show you guys but there are no contams...

But yeah VG you are right man...ps.ovioids myceleium is fuckin strong!!!




Good to hear, i've heard claims that it's possible to inoc. BRF jars with syringes from wild ovoids, and due to their aggressive and fast growth they outpace contams.

Still.. it would be wise to get some on agar and play it safe. If you don't have a PC, i imagine steam sterilization could also work.


--------------------
Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only

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Offlinepfshroomer
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Re: ps. ovioids liquid culture [Re: VirginiaGentleman]
    #18433372 - 06/17/13 06:53 PM (11 years, 6 months ago)

Right...
Im wondering whats best to use when my LC is ready to use...ya know with cubes you use WBS...well with these ovioids should i just grab some old decaying wood(ill try n get the wood they prefer)and then throw that in a jar...PC it,then inoculate!!??
Thanks guys as this is my first wood loving grow


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OfflineMacMerdin
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Re: ps. ovioids liquid culture [Re: pfshroomer]
    #18433579 - 06/17/13 07:39 PM (11 years, 6 months ago)

Quote:

pfshroomer said:
well with these ovioids should i just grab some old decaying wood(ill try n get the wood they prefer)and then throw that in a jar...PC it,then inoculate!!??





Grab the wood and soak it for a day.  Drain it well after.  Mix in moisturized (field capacity) vermiculite with the wood to give the mycelium something to bridge the gaps and also hold moisture.  Just make sure the final result isn't too wet.  PC it and shoot it up.

Once colonized, plant the cake somewhere it will enjoy and grow.


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Invisible36fuckin5
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Re: ps. ovioids liquid culture [Re: MacMerdin]
    #18436046 - 06/18/13 09:58 AM (11 years, 6 months ago)

Quote:

MacMerdin said:
Quote:

pfshroomer said:
well with these ovioids should i just grab some old decaying wood(ill try n get the wood they prefer)and then throw that in a jar...PC it,then inoculate!!??





Grab the wood and soak it for a day.  Drain it well after.  Mix in moisturized (field capacity) vermiculite with the wood to give the mycelium something to bridge the gaps and also hold moisture.  Just make sure the final result isn't too wet.  PC it and shoot it up.

Once colonized, plant the cake somewhere it will enjoy and grow.




No, don't do this at all. One, it's not likely to take off well, and if it does, it's gonna take FOREVER. And when you spawn, that'll also take fucking forever because you don't have many inoculation points.

OP, either do up a bag of sawdust spawn or just go to some kind of grain. Then spawn either of those to woodchips after they colonize.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflineMacMerdin
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Re: ps. ovioids liquid culture [Re: 36fuckin5]
    #18436083 - 06/18/13 10:06 AM (11 years, 6 months ago)

Well,  I don't do liquid cultures but I DO do pf jars directly from spores for woodlovers with wood added.

Please look at my gallery under growing woodlovers and tell me this is not a viable method.  And no.....it doesn't take fucking forever.


--------------------
MacMerdin's Simple Pan-Cakes


Edited by MacMerdin (06/18/13 10:16 AM)

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Invisible36fuckin5
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Re: ps. ovioids liquid culture [Re: MacMerdin]
    #18436126 - 06/18/13 10:19 AM (11 years, 6 months ago)

Quote:

MacMerdin said:
Please look at my gallery under growing woodlovers and tell me this is not a viable method.  And no.....it doesn't take fucking forever.




I didn't say it wouldn't work, I said there are better methods.

And yes, it does take fucking forever compared to using grain. I've tried both.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
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