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OfflineOne Love
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Re: myc questions [Re: One Love]
    #18397493 - 06/10/13 02:02 PM (11 years, 7 months ago)

I should add the only thing I don't PC is coco coir, when I make it I use hot or boiling water. I had long ago read that cococoir is very resistant to contams - would that be true? In any case, cococoir can be found very easily, at the petstore, or you can order things online as I do. The possibilities are endless!


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Edited by One Love (06/10/13 02:47 PM)

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OfflineAsurian
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Re: myc questions [Re: One Love]
    #18397744 - 06/10/13 02:57 PM (11 years, 7 months ago)

I was going to use a premade mixture from my local florist of peatmoss and verm. I'm just really not wanting to have to pc, all that verm. And if I would have to then I don't understand why I wouldnt just do a transfer of the brf before its fully colonized in a sterile environment. Maybe even just getting a bigger jar and putting the full cake in that with more verm to spread futher. My intention.s are to get as much myc with 5cc as possible

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Re: myc questions [Re: Asurian]
    #18397764 - 06/10/13 03:01 PM (11 years, 7 months ago)

so spawn your cakes to a bulk sub, no casing needed


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It doesn't matter what i think of you...all that matters is clean spawn
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InvisiblePastywhyteMDiscord
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Re: myc questions [Re: Asurian]
    #18397799 - 06/10/13 03:08 PM (11 years, 7 months ago)

Quote:

Asurian said:
My intention.s are to get as much myc with 5cc as possible




Then I recommend trying agar and grain. 0.5 cc will be enough to last forever :wink:

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InvisibleNecroMyce
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Re: myc questions [Re: Pastywhyte]
    #18397814 - 06/10/13 03:11 PM (11 years, 7 months ago)

http://www.shroomery.org/forums/showflat.php/Number/14995972/fpart/1/vc/1

You can always try this before you go to a real bulk grow. gives you a little idea of what you'll be doing and is quite simple :laugh:

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OfflineAsurian
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Re: myc questions [Re: NecroMyce]
    #18398916 - 06/10/13 07:15 PM (11 years, 7 months ago)

Is there a way to get agar without going online?

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OfflineCMOS
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Re: myc questions [Re: Asurian]
    #18398929 - 06/10/13 07:19 PM (11 years, 7 months ago)

Yeah just get some light malt extract from a brew supply store and some agar agar from a asian store


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OfflineOne Love
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Re: myc questions [Re: Asurian]
    #18399001 - 06/10/13 07:36 PM (11 years, 7 months ago)

Quote:

Asurian said:
I was going to use a premade mixture from my local florist of peatmoss and verm. I'm just really not wanting to have to pc, all that verm. And if I would have to then I don't understand why I wouldnt just do a transfer of the brf before its fully colonized in a sterile environment. Maybe even just getting a bigger jar and putting the full cake in that with more verm to spread futher. My intention.s are to get as much myc with 5cc as possible




If you really are going to risk transfer with BRF then I think it would be best to let the jar colonize 100% and try cutting out a piece. Although you would have to wait for the new jars to colonize again, it should be faster than doing it from syringe. And also, you would have to sterilize the new jar before putting it in. In that case, put all your materials in a glovebox/your sterile environment, use something sharp and sterile. Should also loosen the jar lids beforehand so you can then open and close quickly as you take out/drop in.
If you were more patient, you could use a very small bit of of the spores and just inoculate the new jars, rather than inoculating four points. It would take longer to colonize, however.

Where are you now in the process? Have you got jars colonizing or have you not started yet? If so, what materials do you have to work with?

To get a lot of yield with what you have I would have to agree with cronicr and just break up your cakes into a bulk sub, using a monotub. It's easy.

I've never done trays as CaspuuuR suggested, but that's also another alternative.

Agar is difficult, especially if you are inexperienced. Best to try other methods first, until you can at the very least be sure your sterile technique is working for you.


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OfflineAsurian
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Re: myc questions [Re: One Love]
    #18406512 - 06/12/13 01:16 AM (11 years, 7 months ago)

Thanks you for the long helpfull reply! It appears mono tub is the best way. I was under the impression a mono tub was a casing?

And what you explained would be the exact process of how I would do the brf 2 brf transfer although I would try to put more than just a little myc to the new also sterile jar to increase colonization rate. Sorry if I don't make since, I am a newbie remember.

Also, if I were to be cultivating illegal shrooms (wich I'm not) I would be exactly 5.5 days after innoc

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Re: myc questions [Re: Asurian]
    #18406533 - 06/12/13 01:23 AM (11 years, 7 months ago)

we don't use the term casing like that, we call them bulk subs or trays but not casings.
casing layers are non nutritious and used to give 99-100%humidity on the surface of your sub to aid in hyphal not formation:cool: this usually consists of a mix of peat moss/verm and some sorta buffer like lime.
that being said ther not needed for bulk subs


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineOne Love
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Re: myc questions [Re: Asurian]
    #18406582 - 06/12/13 01:43 AM (11 years, 7 months ago)

I'm glad to be of help.

A casing is a layer that is placed over the substrate that the mycelium will colonize. It does not have any nutrients ("food") for the mycelium in it.

With cubensis many people will tell you that you don't need a casing layer. I don't use casing, but you can make a monotub with or without it.

If you have already inoculated and are waiting for things to colonize this is the best time to read up and learn and plan what you want to do - your mindset will be quite receptive to information about how you want to approach the fruiting process, and it helps pass the time. You might feel a desire to anxiously decide what method you should use but rather than make a concrete decision read about all your options - but for you I would go with brf to bulk coir monotub! You can make the monotub any size to accommodate how much spawn (spawn being the colonized cakes) you will be using, the bigger the tub in relation to the amount of spawn the longer you will have to wait during the colonization process. You don't want to go too big or it will take too long and contaminants will also have time to flourish.

I had leftovers so I needed to make a small monotub for roughly 2 and a half pints of spawn.
I picked up 6 quart sterilite bin for 3$ at the dollar store.
a pack of ez-felt for 5 dollars for the holes, though most people use polyfill, I like the felt because you can use them for filters on your jars. (They sell sheets for like 50 cents, but I prefer to get the packs because they are sealed up.)
I got a 3 pack of coco coir,(Around 6$?) but for this size I only used a 3rd of a brick. And I am pretty sure they sell single bricks too, for even less.
Then you would also need a roll of micropore/medical tape, to tape up the holes during the colonization period (in the monotub) but this is also the tape used on jar filters, so it's good to have.
It is not mandatory but you should also have a black garbage bag to cover the bottom of the tub. Unless you don't mind pins growing on the bottom.

That's a quick rundown of a monotub(the supplies), should you go that route. Though you might need a bigger or smaller tub depending on your spawn.


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Edited by One Love (06/12/13 01:44 AM)

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OfflineOne Love
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Re: myc questions [Re: cronicr]
    #18406592 - 06/12/13 01:46 AM (11 years, 7 months ago)

Quote:

cronicr said:
we don't use the term casing like that, we call them bulk subs or trays but not casings.
casing layers are non nutritious and used to give 99-100%humidity on the surface of your sub to aid in hyphal not formation:cool: this usually consists of a mix of peat moss/verm and some sorta buffer like lime.
that being said ther not needed for bulk subs




I don't know much about casing layers. I was curious if you were to case a bulk sub (monotub) and it got contaminated would it show on the surface of the casing layer or would it be hidden underneath?


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Re: myc questions [Re: One Love]
    #18406601 - 06/12/13 01:48 AM (11 years, 7 months ago)

there usually right on the surface but if you use the buffer  and pasturize properly you should be more then fine! cubes may not need a casing layer but thats not to say they don't benefit from one


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineOne Love
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Re: myc questions [Re: cronicr]
    #18406617 - 06/12/13 01:54 AM (11 years, 7 months ago)

I've always had this problem with brf cakes, which is one of the reasons why I switched to rye.

Adding the verm "casing" in a brf jar...
The first time I made them it looked fine but I suppose I made the verm layer too deep, and it the jars took forever to colonize. So the second time, I decided to make that layer a little less, but after PCing, the cake shrunk so much that I suppose some of the spores never reached the BRF underneath! Why would they shrink? Should I have packed it or so? Though I'll probably never do BRF again, it'd be good to know ;p


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Re: myc questions [Re: One Love]
    #18406623 - 06/12/13 01:59 AM (11 years, 7 months ago)

you just gently tap it down when your loading, alot of the time the wrong jars are the culprit


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineAsurian
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Re: myc questions [Re: cronicr]
    #18416651 - 06/13/13 11:17 PM (11 years, 7 months ago)

It seems mono tubs are the way to go. The is pf for a little fge and moisture control right?

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