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InvisibleFiend_13
Lord

Registered: 07/13/03
Posts: 424
Help?
    #1839144 - 08/22/03 12:29 AM (14 years, 3 months ago)

Well inculated 12 jars all pressure cooked and inculated in a glove box at the same time. its been 10 days since i inculated and only 2 jars have colonizing going on.. the other 10 show nothing.. no contams no growth of myco... i got the spores syinges from ralphters temp is 81-82 degrees in dark.. anyone know why so many failed to start?


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 11 months
Re: Help? [Re: Fiend_13]
    #1839176 - 08/22/03 12:40 AM (14 years, 3 months ago)

Your stressing about it way too early. Give it a couple more days.


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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Offlineattar
journeyman
Registered: 10/20/02
Posts: 76
Last seen: 12 years, 6 months
Re: Help? [Re: kukusz]
    #1839188 - 08/22/03 12:45 AM (14 years, 3 months ago)

Some say patience is a virtue. Others say it is technique to help one
apprehend the truth.


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 11 months
Re: Help? [Re: attar]
    #1839218 - 08/22/03 01:09 AM (14 years, 3 months ago)

like, whoa...


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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InvisibleSubGen1us
 User Gallery

Registered: 11/26/02
Posts: 3,427
Re: Help? [Re: Fiend_13]
    #1839253 - 08/22/03 01:30 AM (14 years, 3 months ago)

first off, since u pc'ed it then ur probley using some kind of grain tek.
did u shake it? explain a little more.
cause if its grain id expect to see growth alot sooner than 10 days.


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InvisibleFiend_13
Lord

Registered: 07/13/03
Posts: 424
Re: Help? [Re: SubGen1us]
    #1840385 - 08/22/03 01:09 PM (14 years, 3 months ago)

i used BRF and verm... no i did not shake..


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 8 years, 7 months
Re: Help? [Re: Fiend_13]
    #1840390 - 08/22/03 01:12 PM (14 years, 3 months ago)

You let them cool down properly before injecting?


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Sniff, Sniff... What's that smell???... ohhhhh.!!
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Pot Free for another : nevermind.. never made it..


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InvisibleFiend_13
Lord

Registered: 07/13/03
Posts: 424
Re: Help? [Re: PooPs]
    #1840395 - 08/22/03 01:13 PM (14 years, 3 months ago)

yes some of the jars 5 days some 1 day and inbetween just cuz coulnt pc all at one time


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 11 months
Re: Help? [Re: Fiend_13]
    #1840410 - 08/22/03 01:20 PM (14 years, 3 months ago)

There are many variables that dictate whether growth occurs at the same rate as other jars. Since you PC'ed jars on two seperate days, there might be slightly different water content in the two sets. Also, unless you used the same syringe (which I assume you did but might not have) you might get different growth rates. Also, even within the same syringe, sometimes you don't get as many spores to come out into each jar. I would say that your problem is most likely due to the fact that you PC'd on 5 seperate days and innoculated on 1, the water content was most likely slightly different for each jar. Wait another 2 days from now, you should see something.


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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InvisibleDiploidM
Cuban

Folding@home Statistics
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
Trusted Cultivator
Re: Help? [Re: Fiend_13]
    #1840432 - 08/22/03 01:24 PM (14 years, 3 months ago)

Q How much spore solution did you use per hole?

Typically, one to two CCs per jar is recommended.

Q Did you point the needle at the side of the jar or just straight in?

You should try to aim the needle at the side of the jar. This allows the solution to dribble down the side and germinate near the glass where you can see it early rather than deep in the substrate.

Q What's the incubation temperature?

The closer to 85F, the faster things will progress.

Q Did you follow the tek to the letter?

If your substrate is too dry, it can inhibit colonization.

Good luck!

-Diploid


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Republican Values:

1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.

4) We need a smaller, less-intrusive government.


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InvisibleFiend_13
Lord

Registered: 07/13/03
Posts: 424
Re: Help? [Re: kukusz]
    #1840444 - 08/22/03 01:26 PM (14 years, 3 months ago)

that is probably it since when i put together the mixture for the jars it was different times wich means i could of put different amounts of water in the verm. I used annons tek for simple minds and lil kussh says i should of used twice as much BRF in my jars. So that might be it too i guess


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 11 months
Re: Help? [Re: Fiend_13]
    #1840457 - 08/22/03 01:31 PM (14 years, 3 months ago)

Yes, if you also prepared the substrate on seperate days then it's definitely that. I wouldn't worry, you should get growth pretty soon. Even if you used 1/2 the BRF, you should get something, although the flushes and total yield will be smaller obviously.


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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InvisibleFiend_13
Lord

Registered: 07/13/03
Posts: 424
Re: Help? [Re: kukusz]
    #1840481 - 08/22/03 01:37 PM (14 years, 3 months ago)

well the tek says to use x amount of brf i used that much but kussh says if u use that tek use 2x as much


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InvisibleFiend_13
Lord

Registered: 07/13/03
Posts: 424
Re: Help? [Re: Fiend_13]
    #1840522 - 08/22/03 01:56 PM (14 years, 3 months ago)

i also used fine verm in the jars

could that be it?


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Offlinerohitabong
shroomtastic
Registered: 07/14/02
Posts: 120
Loc: DC
Last seen: 14 years, 3 months
Re: Help? [Re: Fiend_13]
    #1841085 - 08/22/03 04:43 PM (14 years, 3 months ago)

just give it time....stress out after another week of no colonization


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In the Time of Chimpanzees I was a Monkey.


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

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