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OfflineNovanity1
Student

Registered: 08/29/10
Posts: 326
Last seen: 10 years, 1 month
Re: Agar issues [Re: spooner]
    #18390540 - 06/08/13 11:52 PM (11 years, 7 months ago)

Quote:

spooner said:
One has sat for over 2 months...I know the agar plates are good cuz I took one and swabbed the inside of my mouth and bacteria started growing on it, I just can't figure this out :/ is there any specific size of a tissue sample I should do?





Sounds like it's the agar. Just because it grew bacteria from your mouth doesn't necessarily mean it has the proper nutrition to grow mycelium. There's some nasty dank ass bacteria in the human mouth which I'm sure can grow on next to nothing .

Here's another test if you have a PC. Put a fully colonized grain on it and see if it shows any growth.

Spores are also a great on agar. They take a while to germinate but it's a good way to get multiple isolates and test which ones yield well and have high potency. You can just scrape them from a print or put a drop from a syringe.

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Offlinespooner
Grow my little babies, GROW!


Registered: 03/09/12
Posts: 259
Last seen: 11 years, 5 months
Re: Agar issues [Re: cronicr]
    #18390551 - 06/08/13 11:55 PM (11 years, 7 months ago)

Trying again, one agar plate has spores strait from a AA+ print and the other has MAZ lc will get pics up and keep you posted


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:sexymeow:

Śānti tumhārē sātha hō

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OfflineNovanity1
Student

Registered: 08/29/10
Posts: 326
Last seen: 10 years, 1 month
Re: Agar issues [Re: spooner]
    #18390605 - 06/09/13 12:06 AM (11 years, 7 months ago)

The LC is a good idea too. If any type of live mycelium doesn't grow on it then it's gotta be the agar.

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: Agar issues [Re: Novanity1]
    #18390890 - 06/09/13 01:20 AM (11 years, 7 months ago)

If it's a tiny, tiny piece of tissue on a hot blade, you could be frying it.  Try a larger piece, dunked in peroxide, from the stipe of a young specimen.

Even with no nutrients, you should still see some growth from the tissue on agar, even if it's just a tiny amount as it's searching for better living conditions.  The only way you could have zero growth is from dead tissue.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlinespooner
Grow my little babies, GROW!


Registered: 03/09/12
Posts: 259
Last seen: 11 years, 5 months
Re: Agar issues [Re: Psilicon]
    #18392373 - 06/09/13 01:09 PM (11 years, 7 months ago)

Well I mean the blade is not hot when I collect the sample lol, but the multistory isn't showing much and it looks like the LCD is starting to do somethin so wel see


--------------------
:sexymeow:

Śānti tumhārē sātha hō

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OfflineSolipsis
m̶a̶d̶ disappointed scientist
Male User Gallery


Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands Flag
Last seen: 1 year, 5 months
Re: Agar issues [Re: spooner]
    #18392554 - 06/09/13 01:53 PM (11 years, 7 months ago)

Do you know what is in the agar? When you say premixed, what is the mix or the use? Maybe there is nutrients lacking or monosaccharides or starch.

Maybe it is better to just treat it like pure agar and add the rest yourself. At the risk of being redundant, that would at least exclude the constitution of the agar being the problem.

If you tried this repeatedly it seems doubtful that you used mushroom tissue that is not viable, if even dried mushrooms can even be viable!

So little else remains as possible reasons. Right?

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