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slaymet69
Mush-brain


Registered: 05/09/06
Posts: 142
Loc: Best state there is, u kn...
Last seen: 4 years, 4 months
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Help: substrate mixed too many tomes!
#18376286 - 06/06/13 03:17 AM (11 years, 7 months ago) |
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Hey everyone I have a question that I can't seem to finud answered! Here it is!
So I have some grow bags that I stated ,there is three and they are filled to 6 inched with substrate,I didn't have much spawn so I used about a pint in each bag,a little under the norm,but it looked to colonize fine fore the first week ,then I shook the whole things up and mixed it all again,it seams to stall for about three days and then started to grow again,not as Vitoria though....
But here's were it's weird,,the bags just didn't seem to get any further,so 4 days ago,I took all 3 bags and mixed them up again( one of the bags was never mixed and was very rhizo,and had myc piss starting to form on the rhizos on the bag,this was the best bag) But my question is did I kill the myc by mixing to much? I must have mixed the 2 bags like 3-4 times total and the other bag maybe once,but there all in the 110 tub w/ liner and lid on with high co2 , but there doesn't seem to be any fast growth like when they first started,only about two micro spots of white myc I can see on the top of all that sub:( Will the myc get more Vitoris and take over and make the sub all white like my regular 2 quartspawn to 2 q sub mix? I mean the sub is now20 days old and doesn't nearly look ready to fruit!?!?
But I don't know I have had a hpoo cake look all brown and pinned the most o have ever seen anything pin before,(granted a lot of them aborted) but still ...this sub doesn't even have like a white fuzziness to it,I'll try and get pics soon,but does this sound like I killed the myc or like I'm gonna have to wait a month plus for full colonize cuz I only used 1-1/2 q spawn on 3 full bags of sub!?!?
Thanks for the REPLYS
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PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 10 years, 8 months
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Re: Help: substrate mixed too many tomes! [Re: slaymet69]
#18376315 - 06/06/13 03:45 AM (11 years, 7 months ago) |
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It seems like a bacterial infection of your spawn. You can mix up spawn more than you suggested w o too many ill effects. It just slows the mycellium by making it take a day to recover. It seems like you mixed up your bag and the bacteria started fighting and stalled your bag but the myc came bk to recover. Your best bet w that bag is to take it outdoors and spawn it outside to anything you can find. It doesnt need to be pasteurized or sterilized. Just mix it up w your contaminated spawn and lay id 4-6 inches deep cover with decomposing matter and let it sit. In 3-4 weeks you should see fruits.
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  WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH
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Mr.E.Man
Spaceman



Registered: 04/02/13
Posts: 486
Loc: Sirius B, RA: 06h 45m 09....
Last seen: 11 years, 4 months
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Re: Help: substrate mixed too many tomes! [Re: slaymet69]
#18376321 - 06/06/13 03:50 AM (11 years, 7 months ago) |
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Quote:
slaymet69 said:
one of the bags was never mixed and was very rhizo,and had myc piss starting to form on the rhizos on the bag,this was the best bag)
First, there is no such thing as mycelium piss. They are metabolites. It's a defence mechanism against bacterias and such.
Quote:
slaymet69 said: But my question is did I kill the myc by mixing to much? I must have mixed the 2 bags like 3-4 times total
Quite possibly. When you mixed the bags, and then 4 days later you mixed again, you may not have given the mycelium time to recuperate and therefor damaged it.
Quote:
slaymet69 said: Will the myc get more Vitoris and take over and make the sub all white like my regular 2 quartspawn to 2 q sub mix?
does this sound like I killed the myc or like I'm gonna have to wait a month plus for full colonize cuz I only used 1-1/2 q spawn on 3 full bags of sub!?!?
It's possible that it will recover and start to colonise again just don't mix them again!
You could of used a few grains to inoculate, as long as the bag was properly sterilised, it doesn't matter, it would colonise. It would just take a very long time.
Try to get some pictures because that will help but personally I would wait it out and see if it recovers in a weeks time. If not then dump the bags.
If it does recover then don't shake/mix the bags until they are about 25-30% and when you do, knead the bag gently to spread to mycelium.
Peace
Mr.E
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-------------------------------------------------------------------Anything above this line and below the previous comment is totally fictional.--------------------------------------I have Amazon Cubensis prints to trade for any other variety of Cubensis...Click Here.------------------------------How I made my Drying Chamber--------------------Experiment 1.0 ------ Experiment 2.0
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 5 years, 13 days
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Re: Help: substrate mixed too many tomes! [Re: Mr.E.Man]
#18376338 - 06/06/13 04:19 AM (11 years, 7 months ago) |
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if it doesnt recover after mixing/shaking thats an indication of contamination.
Also metabolites in excess is also a sign of contamination or stress.
How did you pasturise your bulk substrate, improper pasturisation can cause contamination and also contaminated spawn can cause it
--------------------
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Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
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Franks Simple Coir/Verm Tek
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hyperjump69
Papa Snatchy



Registered: 05/08/11
Posts: 432
Loc: Shakedown Street
Last seen: 9 years, 7 months
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Re: Help: substrate mixed too many tomes! [Re: veda_sticks]
#18376572 - 06/06/13 07:10 AM (11 years, 7 months ago) |
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Quote:
veda_sticks said: if it doesnt recover after mixing/shaking thats an indication of contamination.
Also metabolites in excess is also a sign of contamination or stress.
How did you pasturise your bulk substrate, improper pasturisation can cause contamination and also contaminated spawn can cause it
-------------------- This link will drive you NUTS-------> http://www.youtube.com/watch?v=cKYw0XJfzO4&feature=BFa&list=LLimI46qikD80&index=4
Sorry I don't conform to your idea of reality. Now go away before I bitch-slap you.
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slaymet69
Mush-brain


Registered: 05/09/06
Posts: 142
Loc: Best state there is, u kn...
Last seen: 4 years, 4 months
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Re: Help: substrate mixed too many tomes! [Re: hyperjump69]
#18378844 - 06/06/13 04:50 PM (11 years, 7 months ago) |
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I have a huge 55 gallon steam pasteurizer I made that keeps everything at like 150f. And for 2-3 hours time,then I let sit till next day and spawn,I didn't have much spawn so used like a pint in each bag like I said one bag I only mixed like once and was looking good it just had metabolites around the big rhino fans that were fanning out when they hit the bag,but u could still see a lot of semi-colonized coir,, Not like my elm oyster coir bags that are 100% white, The metabolites I'm talking about almost look like when ganoderma turns yellow in the spawn jars not like bright yellow myc piss on cakes,but I could tell they were metabolites,I hold the bags from the top and check on them like 3 times aday so maybe the bag hanging when I look at it squeezes the rhinos and stresses them out making piss,or maybe they are contamd but idk!!! I know that when u shake a spawn jar it recovers fast in like 3 days ,but after I shake these coir bags it doesn't make anyrhizos after and just seems super slow,I can't take pics cuz I already mixed it in a tub but it's now like 5 days since I put in tub and I can see like 3 little air mycelium reaching up so it looks like it's still alive but it is defiantly not turning white like it's 100% u know,bbut oh well. Its also in my lab with positive pressure and has been in there since pasteurization so contams are usually at a minimum,that's why I don't thinks it's contam,also it doesn't smell bad and no sign of contam except previous piss.but we will see how it goes in a week.
Also when I lifted the tub off the shelve today and looked at it there is like a big earthquake looking crack in the sub the whole length almost like it's dried out but it's not it's pretty moist, But I was thinking does that mean the myc is pretty healthy still because it is keeping the sub together like it's a cake!?! So that's what I'm thinking...it must be healthy if it's keeping the sub together when it sags and it breaks a crack in the sub instead of it just moving around like sand or dirt( sry if it's hard to read I don't know how to explain the crack) looks dry but it's not just looks like it's held together with like roots but there's no roots (ie:threads mycelium rhizos) but thanks for all input I'll try and get pics still
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Help: substrate mixed too many tomes! [Re: Mr.E.Man]
#18379217 - 06/06/13 06:07 PM (11 years, 7 months ago) |
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Quote:
Mr.E.Man said:
First, there is no such thing as mycelium piss. They are metabolites. It's a defence mechanism against bacterias and such.
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Mr.E.Man
Spaceman



Registered: 04/02/13
Posts: 486
Loc: Sirius B, RA: 06h 45m 09....
Last seen: 11 years, 4 months
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Re: Help: substrate mixed too many tomes! [Re: FrankHorrigan]
#18381311 - 06/07/13 01:04 AM (11 years, 7 months ago) |
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Quote:
FrankHorrigan said:

Quote:
RogerRabbit said: The last time I had my microscope out, there was no overt evidence of kidneys, bladder, or any other signs of a urinary tract on mycelium. RR
Quote:
RogerRabbit said: On a mycology help forum, it's hardly pompous to ask folks to use correct terminology so as not to confuse those trying to learn. We school them not to call their substrates 'spawn'. We correct them when they refer to a tray of substrate as a 'casing'. We correct them when they think they can boil grains and get them sterile enough for spawn production, etc. etc. Unless the misuse of terminology gets corrected, it continues unabated. It's no more pompous than using the wrong terms is vulgar. Let's all help the new growers learn, avoiding non-accurate terminology that was disproved decades ago.
Mycelium produces antibiotic compounds that can help ward off infection. Penicillin is an excellent example. If you had the clap, you wouldn't ask your doctor for a shot of myc piss. RR
Hahahaha! Sorry Frank, it's early for me and it was easier to quote this =)
You know it's right....
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-------------------------------------------------------------------Anything above this line and below the previous comment is totally fictional.--------------------------------------I have Amazon Cubensis prints to trade for any other variety of Cubensis...Click Here.------------------------------How I made my Drying Chamber--------------------Experiment 1.0 ------ Experiment 2.0
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Help: substrate mixed too many tomes! [Re: Mr.E.Man]
#18381509 - 06/07/13 02:32 AM (11 years, 7 months ago) |
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Looks like piss, gets called piss. You know i don't care what you copy from hackers collection
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Mr.E.Man
Spaceman



Registered: 04/02/13
Posts: 486
Loc: Sirius B, RA: 06h 45m 09....
Last seen: 11 years, 4 months
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Re: Help: substrate mixed too many tomes! [Re: FrankHorrigan]
#18381705 - 06/07/13 04:57 AM (11 years, 7 months ago) |
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Quote:
FrankHorrigan said: Looks like piss, gets called piss. You know i don't care what you copy from hackers collection 
Hahahaha He loves RR's quotes! I just thought i'd see what it was like to be "one of those guys"
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-------------------------------------------------------------------Anything above this line and below the previous comment is totally fictional.--------------------------------------I have Amazon Cubensis prints to trade for any other variety of Cubensis...Click Here.------------------------------How I made my Drying Chamber--------------------Experiment 1.0 ------ Experiment 2.0
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Help: substrate mixed too many tomes! [Re: FrankHorrigan]
#18381849 - 06/07/13 06:33 AM (11 years, 7 months ago) |
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Quote:
FrankHorrigan said: Looks like piss, gets called piss. You know i don't care what you copy from hackers collection 
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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Mr.E.Man
Spaceman



Registered: 04/02/13
Posts: 486
Loc: Sirius B, RA: 06h 45m 09....
Last seen: 11 years, 4 months
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Re: Help: substrate mixed too many tomes! [Re: PussyFart]
#18381856 - 06/07/13 06:36 AM (11 years, 7 months ago) |
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Quote:
Notahacker420 said:

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-------------------------------------------------------------------Anything above this line and below the previous comment is totally fictional.--------------------------------------I have Amazon Cubensis prints to trade for any other variety of Cubensis...Click Here.------------------------------How I made my Drying Chamber--------------------Experiment 1.0 ------ Experiment 2.0
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Help: substrate mixed too many tomes! [Re: Mr.E.Man]
#18382108 - 06/07/13 08:47 AM (11 years, 7 months ago) |
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slaymet69
Mush-brain


Registered: 05/09/06
Posts: 142
Loc: Best state there is, u kn...
Last seen: 4 years, 4 months
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Re: Help: substrate mixed too many tomes! [Re: FrankHorrigan]
#18396741 - 06/10/13 10:50 AM (11 years, 7 months ago) |
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Lol all these famous faces lol! Well u know I don't give a sit what u guys call it I've been on here a while and for years I have been reading myc piss and metabolites ,I use mike piss,I'm ghetto( u know ,hard from the streets,) lolahhah.but anyway lol this is stupid people acting like children. I mean metabolites:mycpiss :shalomeesa:pizza what everu wanna call it is too ur own.I've herd bolth over the years andi go with myc piss,I know it's not correct but I don't give a shit..WE ALL KNOW WWHAT WERE TALKING ABOUT HERE!! My reasoning why I call it myc piss is because when ever over water or condensation is applied(ie: from timer mess up or whateves)and the mycpiss(metabolites) show up as those nasty yellow droplets. The fruiting chamber starts to smell like piss,like ur waiting at grand central station in the morning before they steam clean all the cardboard condos and hobos away!!!!lol. But anyway u guys waste so much of ur energy trying to get all mad correcting peeps,do something productive and get off the comp!
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the_jerk
return Noob


Registered: 02/13/13
Posts: 105
Last seen: 4 months, 5 days
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Re: Help: substrate mixed too many tomes! [Re: slaymet69]
#18396752 - 06/10/13 10:54 AM (11 years, 7 months ago) |
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Quote:
slaymet69 said: Lol all these famous faces lol! Well u know I don't give a sit what u guys call it I've been on here a while and for years I have been reading myc piss and metabolites ,I use mike piss,I'm ghetto( u know ,hard from the streets,) lolahhah.but anyway lol this is stupid people acting like children. I mean metabolites:mycpiss :shalomeesa:pizza what everu wanna call it is too ur own.I've herd bolth over the years andi go with myc piss,I know it's not correct but I don't give a shit..WE ALL KNOW WWHAT WERE TALKING ABOUT HERE!! My reasoning why I call it myc piss is because when ever over water or condensation is applied(ie: from timer mess up or whateves)and the mycpiss(metabolites) show up as those nasty yellow droplets. The fruiting chamber starts to smell like piss,like ur waiting at grand central station in the morning before they steam clean all the cardboard condos and hobos away!!!!lol. But anyway u guys waste so much of ur energy trying to get all mad correcting peeps,do something productive and get off the comp!
I would call educating people on the correct terminology in this hobby "something productive"
With an attitude like yours I wouldn't expect people to be too helpful in the future.
PS: Reading your post makes my brain hurt.
Edited by the_jerk (06/10/13 10:55 AM)
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Mr.E.Man
Spaceman



Registered: 04/02/13
Posts: 486
Loc: Sirius B, RA: 06h 45m 09....
Last seen: 11 years, 4 months
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Re: Help: substrate mixed too many tomes! [Re: slaymet69]
#18396784 - 06/10/13 11:05 AM (11 years, 7 months ago) |
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Quote:
slaymet69 said: Lol all these famous faces lol! Well u know I don't give a sit what u guys call it I've been on here a while and for years I have been reading myc piss and metabolites ,I use mike piss,I'm ghetto( u know ,hard from the streets,) lolahhah.but anyway lol this is stupid people acting like children. I mean metabolites:mycpiss :shalomeesa:pizza what everu wanna call it is too ur own.I've herd bolth over the years andi go with myc piss,I know it's not correct but I don't give a shit..WE ALL KNOW WWHAT WERE TALKING ABOUT HERE!! My reasoning why I call it myc piss is because when ever over water or condensation is applied(ie: from timer mess up or whateves)and the mycpiss(metabolites) show up as those nasty yellow droplets. The fruiting chamber starts to smell like piss,like ur waiting at grand central station in the morning before they steam clean all the cardboard condos and hobos away!!!!lol.
You sir, just lost my respect.
Pizza? If you call metabolites pizza, then how would other people know what your talking about when you ask for help? Unless you sent a global memo about the new language that you have invented for the mycological dictionary.
I honestly have never smelt this "piss" smell that you are talking about. This happened the other day actually, my tray was wrapped in cling film and when I removed it and sniffed it, there was no smell of piss?
Quote:
slaymet69 said: But anyway u guys waste so much of ur energy trying to get all mad correcting peeps,do something productive and get off the comp!
Us guys are trying to help you understand mycology and be successful you numpty! Not me so much personally because I am still learning myself but I tell you what, Carry on being as disrespectful as you are and your sure to be carrying out the rest of your research alone, unaided by the good people here 
Good Luck fella!
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-------------------------------------------------------------------Anything above this line and below the previous comment is totally fictional.--------------------------------------I have Amazon Cubensis prints to trade for any other variety of Cubensis...Click Here.------------------------------How I made my Drying Chamber--------------------Experiment 1.0 ------ Experiment 2.0
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B_BOY
Phuck Ewe



Registered: 07/17/11
Posts: 2,819
Loc: O
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Re: Help: substrate mixed too many tomes! [Re: slaymet69]
#18396795 - 06/10/13 11:06 AM (11 years, 7 months ago) |
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Quote:
slaymet69 said: Lol all these famous faces lol! Well u know I don't give a sit what u guys call it I've been on here a while and for years I have been reading myc piss and metabolites ,I use mike piss,I'm ghetto( u know ,hard from the streets,) lolahhah.but anyway lol this is stupid people acting like children. I mean metabolites:mycpiss :shalomeesa:pizza what everu wanna call it is too ur own.I've herd bolth over the years andi go with myc piss,I know it's not correct but I don't give a shit..WE ALL KNOW WWHAT WERE TALKING ABOUT HERE!! My reasoning why I call it myc piss is because when ever over water or condensation is applied(ie: from timer mess up or whateves)and the mycpiss(metabolites) show up as those nasty yellow droplets. The fruiting chamber starts to smell like piss,like ur waiting at grand central station in the morning before they steam clean all the cardboard condos and hobos away!!!!lol. But anyway u guys waste so much of ur energy trying to get all mad correcting peeps,do something productive and get off the comp!
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hyperjump69
Papa Snatchy



Registered: 05/08/11
Posts: 432
Loc: Shakedown Street
Last seen: 9 years, 7 months
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Re: Help: substrate mixed too many tomes! [Re: B_BOY]
#18397382 - 06/10/13 01:34 PM (11 years, 7 months ago) |
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Let me pinch myself....
dang dawg- you managed to insult Mycology and homeless people at the same time.... shame on you! Instead of doing this-
You should have been doing this-
Ego and shrooms don't mix. You are excused.
-------------------- This link will drive you NUTS-------> http://www.youtube.com/watch?v=cKYw0XJfzO4&feature=BFa&list=LLimI46qikD80&index=4
Sorry I don't conform to your idea of reality. Now go away before I bitch-slap you.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
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Re: Help: substrate mixed too many tomes! [Re: the_jerk]
#18397401 - 06/10/13 01:40 PM (11 years, 7 months ago) |
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Quote:
the_jerk said:
Quote:
slaymet69 said: Lol all these famous faces lol! Well u know I don't give a sit what u guys call it I've been on here a while and for years I have been reading myc piss and metabolites ,I use mike piss,I'm ghetto( u know ,hard from the streets,) lolahhah.but anyway lol this is stupid people acting like children. I mean metabolites:mycpiss :shalomeesa:pizza what everu wanna call it is too ur own.I've herd bolth over the years andi go with myc piss,I know it's not correct but I don't give a shit..WE ALL KNOW WWHAT WERE TALKING ABOUT HERE!! My reasoning why I call it myc piss is because when ever over water or condensation is applied(ie: from timer mess up or whateves)and the mycpiss(metabolites) show up as those nasty yellow droplets. The fruiting chamber starts to smell like piss,like ur waiting at grand central station in the morning before they steam clean all the cardboard condos and hobos away!!!!lol. But anyway u guys waste so much of ur energy trying to get all mad correcting peeps,do something productive and get off the comp!
I would call educating people on the correct terminology in this hobby "something productive"
With an attitude like yours I wouldn't expect people to be too helpful in the future.
PS: Reading your post makes my brain hurt.
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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slaymet69
Mush-brain


Registered: 05/09/06
Posts: 142
Loc: Best state there is, u kn...
Last seen: 4 years, 4 months
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Re: Help: substrate mixed too many tomes! [Re: cronicr]
#18411506 - 06/12/13 11:19 PM (11 years, 7 months ago) |
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Some ones sensitive!!!!!! :O
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