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OfflineTheBoomking
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Registered: 01/21/11
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Re: Growing from a print... Have a few Questions. [Re: Stropharis]
    #18366466 - 06/04/13 07:45 AM (11 years, 7 months ago)

:macdre:

you know how they do it in cali kids goin dumb thizzin' n shit  :rofldrunk:

haha but in all seriousness folks, sdankys advice doesn't seem too far off too me - that plus agar work, couldn't you clean up your culture and have it free of contaminants and you'd be good to go?

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InvisibleVirginiaGentleman
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
    #18366704 - 06/04/13 09:24 AM (11 years, 7 months ago)

Quote:

VirginiaGentleman said:


I just put some Cinct spores on agar yesterday, if you're interested i can PM some pics when they grow out so you can know what you're looking for





:lmafo:sorry...couldn't help myself




roughly translates into "Clear your inbox honey, I'm gonna send you so many dick pics my junk will have sunburn from the camera flash"  :naughty:





Quote:

TheBoomking said:

haha but in all seriousness folks, sdankys advice doesn't seem too far off too me - that plus agar work, couldn't you clean up your culture and have it free of contaminants and you'd be good to go?





It's feasible, but not sustainable


--------------------
Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only

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Offlineriley.123
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Re: Growing from a print... Have a few Questions. [Re: VirginiaGentleman]
    #18367445 - 06/04/13 12:42 PM (11 years, 7 months ago)

Just prepped my agar with Potato Dextrose (Yeast-extract) Agar (PD(Y)A)... Plus I went out yesterday to my horse stables, and found a few fresh samples to clone. I didn't feel to confident about how old those prints were. Plus I think the old "BLACK" prints were just wishful thinking they're kind of like a black brown. I figured it was better safe than sorry, the new prints are BLACK I put a dot of sharpie next to them and it matches!!(: Yay. So time to start.  :dancer:  :rockon:


--------------------
Theres acid rain in my brain and its killing the flowers in my heart...

I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...

All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.

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OfflinecronicrMFacebook
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
    #18367450 - 06/04/13 12:43 PM (11 years, 7 months ago)

could have used a fruit instead of spores


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlineriley.123
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
    #18367470 - 06/04/13 12:48 PM (11 years, 7 months ago)

I can still do that, I still have the mushrooms...


--------------------
Theres acid rain in my brain and its killing the flowers in my heart...

I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...

All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.

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OfflinecronicrMFacebook
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
    #18367472 - 06/04/13 12:48 PM (11 years, 7 months ago)

awesome!


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlineriley.123
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Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
    #18367500 - 06/04/13 12:56 PM (11 years, 7 months ago)

Should I use the base of the stem, or the gills??? And how long do I let the agar cool before pouring into the dishes? Then how long do I wait to actually place the spores/fruit in the petrie dish?  Sorry thats the part I'm super confused on...


--------------------
Theres acid rain in my brain and its killing the flowers in my heart...

I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...

All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.

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InvisibleStropharis
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
    #18367558 - 06/04/13 01:12 PM (11 years, 7 months ago)

Quote:

riley.123 said:
Should I use the base of the stem, or the gills??? And how long do I let the agar cool before pouring into the dishes? Then how long do I wait to actually place the spores/fruit in the petrie dish?  Sorry thats the part I'm super confused on...





I don't have a write up for cloning yet, but frank has one.

Frank's simple cloning tek

Your last two questions are answered in the write up I previously recommended.

Quote:

Stropharis said:
Stro's Agar Prep
By the time you get this ready, I should have a pictorial on spore to clean culture using agar complete.



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Offlineriley.123
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Registered: 01/21/12
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Re: Growing from a print... Have a few Questions. [Re: Stropharis]
    #18367670 - 06/04/13 01:40 PM (11 years, 7 months ago)

Done!(:


--------------------
Theres acid rain in my brain and its killing the flowers in my heart...

I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...

All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.

Extras: Filter Print Post Top
OfflineTheBoomking
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Registered: 01/21/11
Posts: 836
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Re: Growing from a print... Have a few Questions. [Re: VirginiaGentleman]
    #18375213 - 06/05/13 10:03 PM (11 years, 7 months ago)

agar turns solid  at like 105 F keep it just above to pour and your good, when its solid its pretty much good to go :thumbup:\\

to clone take a tissue sample closest to the bottom of the stem, I would think if you tried to clone from the gills you'd risk germinating spores at the same time kind've sabotaging your clone :shrug:

Quote:

thevirginiagentlemen said:

It's feasible, but not sustainable




how's it not sustainable?

Edited by TheBoomking (06/05/13 10:06 PM)

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InvisibleVirginiaGentleman
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
    #18375275 - 06/05/13 10:20 PM (11 years, 7 months ago)

Quote:

TheBoomking said:

how's it not sustainable?





Make a syringe from a wild contaminated print
use only a few drops from syringe for agar and return to storage
bacteria continues to reproduce while in storage
now you have a shit syringe full of bacteria

Not saying it's not possible, just that there's methods for better results



you're better off just scraping spores onto agar in a SAB

for short term results it doesn't really matter though :shrug:


--------------------
Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only

Edited by VirginiaGentleman (06/05/13 10:21 PM)

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InvisiblePastywhyteMDiscord
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
    #18375276 - 06/05/13 10:21 PM (11 years, 7 months ago)

Misunderstood

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InvisibleVirginiaGentleman
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
    #18375303 - 06/05/13 10:26 PM (11 years, 7 months ago)

yeah you can clean them up, but the syringe is likely to go to shit making it useless in a few weeks


it's all good brah :bigblunt:


--------------------
Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only

Edited by VirginiaGentleman (06/05/13 10:27 PM)

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InvisiblePastywhyteMDiscord
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Re: Growing from a print... Have a few Questions. [Re: VirginiaGentleman]
    #18375322 - 06/05/13 10:31 PM (11 years, 7 months ago)

I hate syringes so as soon as I realized you were discouraging their use I'm like "why am I disagreeing with this?"

Really all that's holding me back from PE is my distrust of anything in a liquid suspension and swabs are hard to find :tongue:

I'm a bit drunk to boot :shrug:

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InvisibleVirginiaGentleman
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Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
    #18375367 - 06/05/13 10:39 PM (11 years, 7 months ago)

after pissing away 2 syringes i made from some giant mutant fruits, i'll never look back.


I finally got results dropping the veil remnants of one of those fruits onto agar, and now it looks like i may loose that grain spawn to bacteria  :ifyoucanawe:


--------------------
Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only

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OfflineTheBoomking
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Re: Growing from a print... Have a few Questions. [Re: VirginiaGentleman]
    #18376824 - 06/06/13 08:48 AM (11 years, 7 months ago)

hahah yeah good point, why make the syringe when you can just scrape/wipe into agar :facepalm3:


What do you mean swabs are hard to find? I only work with pe...

Edited by TheBoomking (06/06/13 08:59 AM)

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InvisibleFrankHorrigan
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
    #18376895 - 06/06/13 09:07 AM (11 years, 7 months ago)

Quote:

Pastywhyte said:
Really all that's holding me back from PE is my distrust of anything in a liquid suspension and swabs are hard to find :tongue:




And swabs usually don't get handled/sterilized/dried properly...every single swab I've gotten from someone else has been covered in bacteria. However, the few I've made myself worked like a champ :shrug:

I don't like spore syringes either but if you want a PE syringe, go get one from a sponsor, it's totally worth it :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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InvisiblePastywhyteMDiscord
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Re: Growing from a print... Have a few Questions. [Re: FrankHorrigan]
    #18377019 - 06/06/13 09:39 AM (11 years, 7 months ago)

Im really tempted but, I seem to have rotten luck with syringes even from sponsors :shrug: of all the syringes I've gotten from sponsors, only one worked, most grew nothing and I had a one beautiful specimen of aspergillus. Really most of my successful grows have been from prints acquired from the good people in the marketplace :super:

Maybe i will take a chance and try once more for some PE or APE before i retire :wink:

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OfflineTheBoomking
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
    #18377045 - 06/06/13 09:47 AM (11 years, 7 months ago)

wow sorry to hear about your bad luck

:astonishedmustache:

yeah I highly recommend getting ape

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InvisiblePastywhyteMDiscord
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
    #18377172 - 06/06/13 10:25 AM (11 years, 7 months ago)

It sounds like a bad thing but I'm not bitter or nothing. If anything my poor luck with syringes only made me determined to learn to use agar and grains. Who knows I might have been content to dick around with PF tek a lot longer otherwise. So from that point of view it was probably a good thing in the long run.

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