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OfflineJessel
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Is this Trich? Is this Agar? **It's Penicillium!**
    #18365682 - 06/04/13 12:29 AM (11 years, 7 months ago)

Is this Trich? Or Penicillin? Or something...worse?!  :excited:
Worry not, I have already made safe transfers away.




Anyways, while I'm here, might as well show some cool Agar porn.
Most of these are transfers.. from a Penis Envy spore syringe -> Agar -> an indeterminate amount of transfers -> this:

This baby took four days to become like this:




Took three sections off this guy:




And turned out these guys. Can you guess which plate is from which section? Btw, I know there is contam. That's why there's plenty more plates.  :spoke:




I don't know if you can make this out, but the mycelium is growing up in the air over a possible contaminant. Sick. As. Fuck.




Couple days back I cut a bacterial contaminated section of a petri and I put it on a new petri as "trash".
24 hours later I fucked up an agar transfer to another petri.. so I decided to put it in the "trash" petri. Here is its progression:
Edit: This dish has pinned!






Let's hope some of these babies can fruit!  :douchewink:

cheers all :thumbup:


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Edited by Jessel (06/10/13 11:13 AM)

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OfflinecronicrMFacebook
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18365740 - 06/04/13 12:47 AM (11 years, 7 months ago)

fuck the penicillin those plates are awesome!


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It doesn't matter what i think of you...all that matters is clean spawn
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Offlinecubenpete
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18365750 - 06/04/13 12:51 AM (11 years, 7 months ago)

:manofapproval:

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OfflineJessel
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Re: Is this Trich? Is this Agar? [Re: cronicr]
    #18365762 - 06/04/13 12:55 AM (11 years, 7 months ago)

:awetongue:

Wait, so it is penicillin?


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InvisibleStropharis
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18365831 - 06/04/13 01:23 AM (11 years, 7 months ago)

Nice agar shots OP!

Penicillin are the antibiotics that come from Penicillium, thats what it looks like to me but I can't be certain.

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Re: Is this Trich? Is this Agar? [Re: Stropharis]
    #18365843 - 06/04/13 01:28 AM (11 years, 7 months ago)

that does look like aspergillus penicillium, maybe cross refrence that in the contam forum(i think tmethyl has a shot of it too)


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineJessel
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Re: Is this Trich? Is this Agar? [Re: Stropharis]
    #18365859 - 06/04/13 01:36 AM (11 years, 7 months ago)

Quote:

Stropharis said:
Penicillin are the antibiotics that come from Penicillium




Oooh, lol. That's why Google images weren't helping much.. too many pics of pills and not of petris!

I think it is aspergillus penicillium. :thumbup:


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OfflineJessel
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18367372 - 06/04/13 12:19 PM (11 years, 7 months ago)



So this strain has almost completely taken over all contams on the plate.
When mycelium has done this, does this alter its fruiting ability/genetics/chemical make-up?

I think I'm gonna put this in a couple jars and try and fruit it... I don't know if much will differ from a plate that didn't have to fight to survive :shrug:


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OfflineKizzle
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18367489 - 06/04/13 12:53 PM (11 years, 7 months ago)

It's green :lol: It's literally impossible to identify a mold without a microscope and considering it's on agar there's not even much basis for a good guess.


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InvisibleFrankHorrigan
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Re: Is this Trich? Is this Agar? [Re: Kizzle]
    #18367533 - 06/04/13 01:05 PM (11 years, 7 months ago)

You can ID trich easy enough- eats everything, including your mycelium, moves fast, white, bubbly, etc.

OP, out of curiousity whats your agar recipe?


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Yes, you can bump my old threads with a question.
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Frank's tips and tricks. Updated on 3/21/14
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OfflineJessel
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Re: Is this Trich? Is this Agar? [Re: FrankHorrigan]
    #18367599 - 06/04/13 01:25 PM (11 years, 7 months ago)

Frank, I use Malt Extract Agar. My first Agar run, I used 50g for 1 liter, however I noticed that more MEA per liter of water tends to make the mycelium cottony and spread out slower.

So the plates you see in the OP, I used less MEA to see how it changes mycelial growth... I used about ~35-40g per liter, err on the lower side.

Using this less recipe, I believe, is what allowed one of the plates to grow so fast in just 4 days! :thumbup:


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InvisibleFrankHorrigan
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18367612 - 06/04/13 01:28 PM (11 years, 7 months ago)

That'll do it.  I was just curious, I was banking on it being a low-nute agar.

I just use the standard formula, 50g -> 1L. I don't really try to get my strains to "run" before they hit the grains though.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflineJessel
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Re: Is this Trich? Is this Agar? [Re: FrankHorrigan]
    #18367647 - 06/04/13 01:35 PM (11 years, 7 months ago)

Quote:

FrankHorrigan said:
I just use the standard formula, 50g -> 1L. I don't really try to get my strains to "run" before they hit the grains though.




I see. Well as long as you're using the same formula every time for all strains.. that will set up a good standard to see which one performs the best.

I think I might stick around 40g per liter, just cause I like to stare at my plates and pretend I can see them growing.


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InvisibleFrankHorrigan
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18367655 - 06/04/13 01:37 PM (11 years, 7 months ago)

Yeah, definitely man.

I just have so much going all at once on such a regular basis that I don't mind an extra couple days for a full plate. I like to see them go crazy when they hit the grains :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflineJessel
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Re: Is this Trich? Is this Agar? [Re: FrankHorrigan]
    #18367668 - 06/04/13 01:40 PM (11 years, 7 months ago)

Quote:

FrankHorrigan said:
I like to see them go crazy when they hit the grains :thumbup:




:awethumb:


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InvisibleZarotti
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18367832 - 06/04/13 02:16 PM (11 years, 7 months ago)

Quote:

Jessel said:

So the plates you see in the OP, I used less MEA to see how it changes mycelial growth... I used about ~35-40g per liter, err on the lower side.

Using this less recipe, I believe, is what allowed one of the plates to grow so fast in just 4 days! :thumbup:




You use ~35 g of malt per 1000 ml
        3,5 g per 100 ml

and how much agar do you use per 1000 ml ?


I tend to get thin cottony
non rhyzomorphic mycelium on my agar all the time...

Would trying out your agar recipe improve my mycelium growth?

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InvisibleFrankHorrigan
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Re: Is this Trich? Is this Agar? [Re: Zarotti]
    #18367847 - 06/04/13 02:18 PM (11 years, 7 months ago)

Quote:

Zarotti said:
Quote:

Jessel said:

So the plates you see in the OP, I used less MEA to see how it changes mycelial growth... I used about ~35-40g per liter, err on the lower side.

Using this less recipe, I believe, is what allowed one of the plates to grow so fast in just 4 days! :thumbup:




You use ~35 g of malt per 1000 ml
        3,5 g per 100 ml

and how much agar do you use per 1000 ml ?


I tend to get thin cottony
non rhyzomorphic mycelium on my agar all the time...

Would trying out your agar recipe improve my mycelium growth?




No, he uses 35-40g malt extract agar (MEA) to 1000mL.  Already mixed, I would imagine.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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InvisibleZarotti
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Re: Is this Trich? Is this Agar? [Re: FrankHorrigan]
    #18367854 - 06/04/13 02:19 PM (11 years, 7 months ago)

Quote:

FrankHorrigan said:
Quote:

Zarotti said:
Quote:

Jessel said:

So the plates you see in the OP, I used less MEA to see how it changes mycelial growth... I used about ~35-40g per liter, err on the lower side.

Using this less recipe, I believe, is what allowed one of the plates to grow so fast in just 4 days! :thumbup:




You use ~35 g of malt per 1000 ml
        3,5 g per 100 ml

and how much agar do you use per 1000 ml ?


I tend to get thin cottony
non rhyzomorphic mycelium on my agar all the time...

Would trying out your agar recipe improve my mycelium growth?




No, he uses 35-40g malt extract agar (MEA) to 1000mL.  Already mixed, I would imagine.





Thx

so thats 50/50 mix of agar and malt?

18 g of malt and 18 g of agar to 1000 ml of water?

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OfflineJessel
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Re: Is this Trich? Is this Agar? [Re: Zarotti]
    #18368471 - 06/04/13 04:29 PM (11 years, 7 months ago)

I use this exactly:
http://fungi.com/product-detail/product/enriched-malt-extract-agar-1-pound.html

Seems a lot easier than running around trying to get all the ingredients, if you got the money to afford less hassle.

Also, I don't use a full liter of water for my dishes... I try to pour 25 dishes at one time, but I find 1 liter of water is unnecessary for that amount. My plates get these thick layers of agar, and when transferring sections off, sometimes its so thick it touches the petri cover.
I use approximately 800mL of water with about ~35g of MEA.

But I haven't done much Agar work.. this is only my third Agar cook/pour.

Hope any of that helps. :smile:


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OfflineJessel
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Re: Is this Trich? Is this Agar? [Re: Jessel]
    #18396822 - 06/10/13 11:16 AM (11 years, 7 months ago)

Just posting here to show Shroomery this contam "trash" plate. Has a beautiful pin growing from it. Thick stem and very fast growth. Looks like this mycelium is a  :eyemonster:



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