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Offlinenintendude7cubed
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Stupid newbie mistake redemption...
    #18365056 - 06/03/13 10:14 PM (11 years, 7 months ago)

Hey guys, so I'm growing for the first time and spent hours looking around at how to go about growing the most successfully with PF tek. I ended up getting most of my info from a quite popular instructional video, and more detailed maintenance ideas here at the shroomery. but this info load didn't exactly include how long to wait after steam sterilizing to inoculate the jars. Bottom line: I didn't wait long enough.

After about 5 days of no progress, I realized I made this vital error by looking more into detail on the first stages of colonization, and coming across a post saying to wait at least 6 hours for the jars to cool. So in summary, I let my jars sit for 5 days with my substrate ready to go and had to re-inoculate as many jars as I could with the extra spore I had left in my syringe that I was going to use for an experimental substrate (inoculating only one hole per jar to cover more jars).

My question is this: what do you think the likelihood of these (1/2 pint) jars still being able to colonize successfully in any given amount of time? Should I wait and see? Or should i just start over as I am completely screwed?

Note: I used 1 cup BRF / 1 cup water / 1 cup verm in the jars if that matters

My thoughts based on my own knowledge from reading around, are that letting the jars sit for too long without any action messed with the moisture content, which could screw me over when it comes time for fruiting, but then again it could also just slow the growth and it would be fine since I'm dunking the cakes. I'm just highly inexperienced and would really appreciate input. I plan on growing more in the future whether or not these cakes turn out. The project, to me, is as exciting as the end product, and having such a large support-site makes it a really inviting hobby.

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OfflinecronicrMFacebook
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Re: Stupid newbie mistake redemption... [Re: nintendude7cubed]
    #18365070 - 06/03/13 10:17 PM (11 years, 7 months ago)

you forgot a cup of verm:crazy:


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It doesn't matter what i think of you...all that matters is clean spawn
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Offlinekinkaku
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Re: Stupid newbie mistake redemption... [Re: cronicr]
    #18365081 - 06/03/13 10:20 PM (11 years, 7 months ago)

Quote:

cronicr said:
you forgot a cup of verm:crazy:




beat me to it lol


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OfflineJman420
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Re: Stupid newbie mistake redemption... [Re: kinkaku]
    #18365095 - 06/03/13 10:23 PM (11 years, 7 months ago)

No he didn't? maybe i'm blind lol.

Edit: My bad, you ment 2 cups of verm instead of one lol. :jah:


--------------------
            --My Everything You Need To Know Tek--

Disregard the haters and "know it alls", it is everything you need to know TO GET STARTED.

If you want to become an expert, you'll need to do a lot more research.
-----------------------------------------------------------------------
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-----------------------------------------------------------------------
                            :amanita2::syringe: :mushroom2::prance::mushroom2::cubie::sporedrop:

Edited by Jman420 (06/03/13 10:23 PM)

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OfflineJohnny Quest
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Re: Stupid newbie mistake redemption... [Re: nintendude7cubed]
    #18365122 - 06/03/13 10:29 PM (11 years, 7 months ago)

There are too many factors for me to tell you if your cakes will grow...five days is a long time in the microbiotic world but if your sterile technique was on point then they could certainly be successful. I'd say you have hope.  Most importantly you're learning from your mistakes right now. Hang in there and keep on learning (no seriously...keep learning, get familiar with every vid and tech there is on your style of growing!)
 
oh and about the moisture...with pf jars there are only 2 holes at the top so perhaps not much water vapor escaped...depends on the temp and relative humidity where you're at.  Some stores sell pre-made pf jars that sit on shelves for god knows how long and there still is some marginal success with those kits.


--------------------
Studying mycology since: August, 2008

A typical monotub grow of a multi-spore rye spawn with some Golden Teachers: inoculated on day one and saw first signs of growth on day 5. On day 17 jars were 15% - 30% colonized so shook em up. On day 25 I mixed the spawn and sub in the monotub and on day 50 harvested the first flush. Just under 4 ounces dry in 1 month 19 days!

One Love!

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Offlinenintendude7cubed
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Re: Stupid newbie mistake redemption... [Re: Johnny Quest]
    #18365417 - 06/03/13 11:29 PM (11 years, 7 months ago)

Quote:

Johnny Quest said:
There are too many factors for me to tell you if your cakes will grow...five days is a long time in the microbiotic world but if your sterile technique was on point then they could certainly be successful. I'd say you have hope.  Most importantly you're learning from your mistakes right now. Hang in there and keep on learning (no seriously...keep learning, get familiar with every vid and tech there is on your style of growing!)
 
oh and about the moisture...with pf jars there are only 2 holes at the top so perhaps not much water vapor escaped...depends on the temp and relative humidity where you're at.  Some stores sell pre-made pf jars that sit on shelves for god knows how long and there still is some marginal success with those kits.




(I definitely meant 2 cups of verm, sorry 'bout that.)
Fortunately with it being just the beginning of summer, my patience is at its highest and I'm exploring the possibilities as well as enhancing my own knowledge quite diligently.

Fortunately since I had the holes covered with masking tape, I probably didn't lose much moisture at all. Luckily It's been raining for like 3 of the 5 days outside so relative humidity has been good for keeping it moist, so that is reassuring.
Having the inoculation in an open air environment probably reduced my chances for success, but everything else made up for it. I bleached a wooden table really well (which i imagine, judging by the heavy fumes, somewhat cleaned up the air), laid wax paper on top of that after washing my hands with anti bacterial soap, put on rubber gloves and went to work flaming the needle red hot for every jar. I wasn't in a kitchen or a bathroom either which i think would tend to be more risky with bacteria in the air since the rooms deal with food and human waste. I will come back with pictures in the coming several weeks to show what all happens. Thanks for the insight :smile:. If i get at least a few out of the 10 jars I managed to inoculate to be clean cakes, I will be thrilled.

I know one tek that I'm interested in experimenting with next time i get some spores is some sort of liquid tek with an organic syrup or honey (ideally syrup since i think it is cheaper). I like the idea because you can see your mycelium as soon as it gets the tiniest hint of growth.

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OfflinecronicrMFacebook
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Re: Stupid newbie mistake redemption... [Re: nintendude7cubed]
    #18365428 - 06/03/13 11:32 PM (11 years, 7 months ago)

just wait it out then man your fine!i would rather see you look into agar fisrt it's much more rewarding


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineJman420
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Re: Stupid newbie mistake redemption... [Re: cronicr]
    #18365442 - 06/03/13 11:36 PM (11 years, 7 months ago)

Also, 5 days is a very short amount of time. It could take it much longer than that to start seeing VISIBLE signs of growth. I say "visible" because sometimes the brf cakes will colonize from the inside-out.

I would say wait about 3 weeks for visual signs of growth, if there are none, then worry. :grin:

:goodluck:

Patience is key.


--------------------
            --My Everything You Need To Know Tek--

Disregard the haters and "know it alls", it is everything you need to know TO GET STARTED.

If you want to become an expert, you'll need to do a lot more research.
-----------------------------------------------------------------------
                  Open Your Mind To A New Future
-----------------------------------------------------------------------
                            :amanita2::syringe: :mushroom2::prance::mushroom2::cubie::sporedrop:

Edited by Jman420 (06/03/13 11:36 PM)

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Offlinekinkaku
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Re: Stupid newbie mistake redemption... [Re: nintendude7cubed]
    #18365445 - 06/03/13 11:36 PM (11 years, 7 months ago)

as long as you where super clean it should work for you also bacteria isn't in the air my friend but im sure you already knew that:thumbup:
if you do LC make sure to test them on a BRF jar before you actually us it to knock up your real jars. I just lost 10 jars of WBS do to a bad LC. it was a stupid mistake, but it did shave a week of my colonizing times.


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Offlinenintendude7cubed
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Re: Stupid newbie mistake redemption... [Re: Jman420]
    #18365527 - 06/03/13 11:57 PM (11 years, 7 months ago)

Quote:

Jman420 said:
Also, 5 days is a very short amount of time. It could take it much longer than that to start seeing VISIBLE signs of growth. I say "visible" because sometimes the brf cakes will colonize from the inside-out.

I would say wait about 3 weeks for visual signs of growth, if there are none, then worry. :grin:

:goodluck:

Patience is key.




I was going to wait much longer, but after reading the post about how long you should wait for the jars to cool, I knew it would've been wasted days. The post said wait 6 hours at the very least, I waited less than 1 hour. Hopefully now I will see quicker results to make up for lost time, but i read somewhere that golden teachers tend to take longer to germinate, but i try not to get wrapped up in all that strain junk and just focus on my patience and general gain in effective growing technique.

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Offlinenintendude7cubed
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Re: Stupid newbie mistake redemption... [Re: cronicr]
    #18365594 - 06/04/13 12:10 AM (11 years, 7 months ago)

Quote:

cronicr said:
just wait it out then man your fine!i would rather see you look into agar fisrt it's much more rewarding




I can totally see how agar can be rewarding after reading up briefly on it. I think it is a good idea because if i am not under the right conditions to be growing shrooms and need to take a break (Like if i were to move to a different place, which happens often since i'm a student), I don't have to waste time and money on waiting for more spores when i could use this self-produced media. And it would be worth investing in a cheap pressure cooker to make sure this goes well since agar seems to demand one.

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Re: Stupid newbie mistake redemption... [Re: nintendude7cubed]
    #18365624 - 06/04/13 12:15 AM (11 years, 7 months ago)

:headbang3:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinenintendude7cubed
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Re: Stupid newbie mistake redemption... [Re: cronicr]
    #18425398 - 06/15/13 10:36 PM (11 years, 7 months ago)

UPDATE!!!: I checked my jars like 3 days after the new inoculation and there was a barely detectable spot forming on a few of my jars. Then I went to visit parents and stayed the week for my little sister's high school graduation. Came home to this:



I closely inspected these and none of them look contaminated so far. There were 2 additional jars I had attempted to revive that I didn't include because they didn't take yet. But I'm super happy about this progress and that I hadn't completely thwarted my project. Unfortunately the consequence is that the wait is gonna be a bit longer until birthing since the mycelium is only growing from one side. Oh well. The payoff will be huge!!!:mushroom2::mushroom2::mushroom2:

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