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OfflineKingCloyster
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Master Slant Tubes.... which malt extract to use? how to adjust amounts
    #18364425 - 06/03/13 08:16 PM (10 years, 11 months ago)

What type of malt extract should I use for a master slant tube?

I think this is the right recipe: 10 grams agar agar powder, 5 grams malt(is it more or less for diff kinds of malt? Or does only one kind work?) Per 500 mL


I see a lot of lme available on eBay, but then you start getting into wheat, amber, dark... Idk which one to choose

Decisions! Decisions!


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Offlinematsc
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Re: Master Slant Tubes.... which malt extract to use? how to adjust amounts [Re: KingCloyster]
    #18364599 - 06/03/13 08:48 PM (10 years, 11 months ago)

2% agar sounds right for slants, so that works. I personally use an extra light dry malt extract (bought from a local home brewers shop) for my mixes and it works just fine. Ive also used a "pilsen" dry malt before that worked fine. Basically I just went for the lightest color malt I could grab, both for sugar integrity and for aesthetics.


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OfflineOICU812
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Re: Master Slant Tubes.... which malt extract to use? how to adjust amounts [Re: matsc]
    #18366192 - 06/04/13 05:19 AM (10 years, 11 months ago)

Extra light malt extract is all I ever use because you want to be able to see through the bottom of the plate of agar to examine your specimen. 

Here is a recipe from Fastfred's Media Cookbook:


Long-term Preservation Medium (FDA M85)
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3 g Yeast extract, 0.3%
10 g Peptone
30 g NaCl
3 g Agar
1 L Distilled water

Heat to dissolve ingredients. Dispense 4 ml portions to 13 x 100 mm screw-cap tubes. Autoclave 15 min at 121°C. Cool and tighten caps for storage. No pH adjustment is necessary. [1]


I use 17ml in a 20x150 tube as taught at Aloha.  I also add a wooded stick (coffee stir).  Rather than screw caps, I use Kim Caps and parafilm them to the culture tube (which allows for air exchange).



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OfflineKingCloyster
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Re: Master Slant Tubes.... which malt extract to use? how to adjust amounts [Re: OICU812]
    #18367735 - 06/04/13 01:55 PM (10 years, 11 months ago)

I just settled for light malt extract. thanks guys


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