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Droned
Stranger


Registered: 03/17/10
Posts: 242
Last seen: 11 years, 4 months
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soaking grains for hatching endospores?
#18358902 - 06/02/13 03:58 PM (11 years, 7 months ago) |
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I've read alot of conflicting info on this, Ive heard that this is outdated info and that a PC at 15 psi for an 90 mins is sufficient to kill endospores?
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
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Re: soaking grains for hatching endospores? [Re: Droned]
#18359947 - 06/02/13 08:42 PM (11 years, 7 months ago) |
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It's irrelevant. The main reason for soaking grains is to hydrate them slowly so they don't burst in the pressure cooker. The old way from years back where we would simply put the grains and water in a jar and then toss it into the PC resulted in a lot of busted kernels and spilled starch. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Tedkaz
Mr.make do


Registered: 04/23/12
Posts: 53
Last seen: 8 years, 11 months
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Re: soaking grains for hatching endospores? [Re: RogerRabbit]
#18428106 - 06/16/13 02:52 PM (11 years, 6 months ago) |
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Does that mean that one could potentially PC dry grains, and still kill the endospores? We only get 6 quart pressure cookers where i live, which can only handle three pints at the time. If i could pc dry grains in a bag and then hydrate, load and steam in my 40 quart kettle, that would easily amount to 10 quart jars per pc run.
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TheReckoning
:)

Registered: 05/13/09
Posts: 411
Last seen: 2 years, 9 months
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Re: soaking grains for hatching endospores? [Re: Tedkaz]
#18428393 - 06/16/13 04:04 PM (11 years, 6 months ago) |
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Quote:
Tedkaz said: Does that mean that one could potentially PC dry grains, and still kill the endospores? We only get 6 quart pressure cookers where i live, which can only handle three pints at the time. If i could pc dry grains in a bag and then hydrate, load and steam in my 40 quart kettle, that would easily amount to 10 quart jars per pc run. 
You hydrate before PC'ing. Once you PC, that jar or bag should never be opened until it is colonized or you are in front of a laminar flow hood. Attempting to hydrate after PC'ing will undo your entire sterilization process.
-------------------- A scapegoat, for all your insecurities. Excuse me; a hero; a man made god; for all your insecurities - Remembering Never
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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
Posts: 920
Last seen: 6 months, 12 days
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Re: soaking grains for hatching endospores? [Re: TheReckoning]
#18428804 - 06/16/13 05:34 PM (11 years, 6 months ago) |
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Quote:
TheReckoning said:
You hydrate before PC'ing. Once you PC, that jar or bag should never be opened until it is colonized or you are in front of a laminar flow hood, glove box, or still air box. Attempting to hydrate after PC'ing will undo your entire sterilization process.
Fixed that for you... There are a ton of peeps that do g2g transfers without a lfh. (Some even in open air, but I personally wouldn't try that)
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Tedkaz
Mr.make do


Registered: 04/23/12
Posts: 53
Last seen: 8 years, 11 months
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Re: soaking grains for hatching endospores? [Re: Droned]
#18428830 - 06/16/13 05:40 PM (11 years, 6 months ago) |
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Quote:
6DEA6 said: Nope it wont work
Yes, that is my primary suspicion, but i would still like to know why.
TheReckoning said:
"You hydrate before PC'ing."
Yes, that is standard procedure for grains.
"Once you PC, that jar or bag should never be opened until it is colonized or you are in front of a laminar flow hood. Attempting to hydrate after PC'ing will undo your entire sterilization process."
The reason we sterilize grains with pressure cookers or autoclaves is primarily because of the bacterial endospores, as they can survive regular boiling temperatures. The other possible contaminants easily die at regular boiling temperatures. So if i was to kill the endospores in the grains by pc, exposing the grains to the environment in my house would not matter if i was to hydrate and steam them right afterwards.
Endospores are more easily killed when hydrated. What i want to know is whether that excludes killing them with heat in their un-hydrated form.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 6 hours, 16 minutes
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Re: soaking grains for hatching endospores? [Re: Tedkaz]
#18428857 - 06/16/13 05:47 PM (11 years, 6 months ago) |
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your way overthinking this, put some dry popcorn in the pc and give'er shit, be sure to have your butter ready i'm just kidding man here give this a read http://www.shroomery.org/forums/showflat.php/Number/14053216#14053216
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Tedkaz
Mr.make do


Registered: 04/23/12
Posts: 53
Last seen: 8 years, 11 months
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Re: soaking grains for hatching endospores? [Re: cronicr]
#18437641 - 06/18/13 03:30 PM (11 years, 6 months ago) |
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Thanks for the link, i read it. Still seems that endospores is something to worry about though, lest we could just steam or jars instead of pressure cooking them, right?
And it didn't seem to say anything about PC'ing dry grains either.
I wonder what RR would say about this.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 6 hours, 16 minutes
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Re: soaking grains for hatching endospores? [Re: Tedkaz]
#18437680 - 06/18/13 03:37 PM (11 years, 6 months ago) |
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bottom line we soak to hydrate slowly so we don't burst to many kernels, too long of a soak can be counterproductive, and grains should be soaked before pc'ing because it's the steam from the inside that sterilizes. at least that makes sense to me
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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