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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Growing from a print... Have a few Questions.
#18358453 - 06/02/13 01:53 PM (11 years, 7 months ago) |
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Okay, so I have like 20 good BLACK Spore prints that I got from some cinctulus. I also still have the dried mushrooms.
I really want to grow some mushrooms already!!! I live in California so I can't order and spore syringes, and California isn't really the best hunting place either However, I found what the good people of Shroomery confirmed to be Subbs; Took good prints of them, and fully dried and stored the shrooms. I have read and read and read about how to grow out of popcorn, rice flour, grain, horse shit, cow shit, and everything in between... I JUST WANT TO START MY GROW! The problem is I don't have agar as of right now, and still live with my parents so getting supplies is a little hard. Will someone please offer some advice on what I could possibly do to make this happen or am I just at a loss until I can get a spore syringe...???
Thanks so much, Riley Ann.(:
Ps. The pics of the shrooms are in my gallery(:
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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Uhurungus
Avoiderer of Bullshit


Registered: 04/20/13
Posts: 838
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18358707 - 06/02/13 03:06 PM (11 years, 7 months ago) |
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in the top right corner is a search box. type in "making spore syringe" and start looking at the threads that come up
-------------------- I'm never 100% on anything, just close enough to risk looking foolish.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 27 days
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Re: Growing from a print... Have a few Questions. [Re: Uhurungus]
#18358743 - 06/02/13 03:14 PM (11 years, 7 months ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: PussyFart]
#18359422 - 06/02/13 06:28 PM (11 years, 7 months ago) |
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you're not going to want to make a syringe from wild prints, it's likely they also have traces of other mold, fungus, or bacteria which will lead to contamination.
Agar work is necessary, if you dont have access to it - wait until you do
also, be very selective with your prints - some of your pics looked like there are a few foe's mixxed in
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Are your parents cool with you growing mushrooms? If not, you really shouldn't be growing them at their house anyway.
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18359586 - 06/02/13 07:11 PM (11 years, 7 months ago) |
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i still don't understand why people use their full name, location, and interest in their profile - then post about cultivating 
When you turnin 18 gurl  https://www.facebook.com/riley.sebach/photos
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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Quote:
VirginiaGentleman said: i still don't understand why people use their full name, location, and interest in their profile - then post about cultivating 
When you turnin 18 gurl  https://www.facebook.com/riley.sebach/photos
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 27 days
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18359721 - 06/02/13 07:49 PM (11 years, 7 months ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Uhm...... yeahhhhhhh.
Edited by riley.123 (06/03/13 02:57 PM)
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Stropharis



Registered: 04/02/07
Posts: 1,854
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18362962 - 06/03/13 03:04 PM (11 years, 7 months ago) |
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Quote:
VirginiaGentleman said: i still don't understand why people use their full name, location, and interest in their profile - then post about cultivating 
When you turnin 18 gurl  https://www.facebook.com/riley.sebach/photos
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18363001 - 06/03/13 03:10 PM (11 years, 7 months ago) |
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Well anyyyyyways. I got my hands on some agar powder. I also have some petrie dishes, sterile hay meant for infusion kits, and I have packaged sterile soil... I can grow outside or inside, but I'd prefer outside. Plus I have unlimited access to cow, or horse dung. My backyard is grass, and it's shaded... I know the general different ways I can go at this, but are there any tips you guys can offer me, seeing as how I'm in So cal and it's so damn hot! Please, any advice you have! I will do anything to grow, I've researched, and hunted for about a year now and just want to start already. If theres anything completely necessary I'm missing I will find a way to get it... haha
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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Stropharis



Registered: 04/02/07
Posts: 1,854
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18363028 - 06/03/13 03:16 PM (11 years, 7 months ago) |
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Prep some agar now, here are some recipes, then get your culture cleaned up and ready for grains.
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18365260 - 06/03/13 11:00 PM (11 years, 7 months ago) |
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Quote:
riley.123 said: Well anyyyyyways. I got my hands on some agar powder. I also have some petrie dishes Please, any advice you have!
you should also pick up a pressure canner/cooker (PC), make a Still air box (SAB), raid your USPS or FedEx location for tyvek envelopes, and ditch the petris, get a pack of Ball elite 1/2pint wide mouth jars instead
they look like this http://www.mountainfeed.com/images/ball-half-pint-elite-case.jpg
I just put some Cinct spores on agar yesterday, if you're interested i can PM some pics when they grow out so you can know what you're looking for
Quote:
Stropharis said: here are some recipes
I've had good results such as germination in 2-3 days with cubes, ovoids, and allenii on 1:1 LME/agar-agar, 10min simmer, 24cc's of media in Ball elite 1/2pint wide mouths, PC'd for 30-35min
also if you lack a scale 1/4 tsp of agar or LME is roughly 1g
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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Stropharis



Registered: 04/02/07
Posts: 1,854
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Quote:
VirginiaGentleman said: ditch the petris, get a pack of Ball elite 1/2pint wide mouth jars instead
I disagree.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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Quote:
VirginiaGentleman said: When you turnin 18 gurl 
Quote:
VirginiaGentleman said:
I just put some Cinct spores on agar yesterday, if you're interested i can PM some pics when they grow out so you can know what you're looking for
sorry...couldn't help myself
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Jman420
MagicJ



Registered: 12/23/12
Posts: 866
Loc: On a Cloud
Last seen: 4 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18365892 - 06/04/13 01:49 AM (11 years, 7 months ago) |
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-------------------- --My Everything You Need To Know Tek--
Disregard the haters and "know it alls", it is everything you need to know TO GET STARTED.
If you want to become an expert, you'll need to do a lot more research.
-----------------------------------------------------------------------
Open Your Mind To A New Future
-----------------------------------------------------------------------
    
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sdanky
Matt



Registered: 06/04/13
Posts: 2
Loc: Work
Last seen: 11 years, 1 month
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18365907 - 06/04/13 01:54 AM (11 years, 7 months ago) |
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This is obvious.
Materials: 1. Distilled Water or Tap water that had been boiled for at least 10 minutes.
2. Syringe
3. Spore Print
Steps:
- Get a sterile area (Preferably a glove-box or a Hood).
- Scrape the Spores into the water (That is sterile and cooled down).
- Suck the water into the syringe and press out again into the container where the water was as to get all the spores off the sides.
- Done, you have a syringe.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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Re: Growing from a print... Have a few Questions. [Re: sdanky]
#18365930 - 06/04/13 02:09 AM (11 years, 7 months ago) |
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yes thats how you would make a nice n dirty syringe wild prints are never clean neither is the mushroom, inside and out so agar is is the more logical route. welcome to shroomery 2 btwsdanky(and i don't bother chasing spores off the sides, once it hit's my syringe it doesn't come out till inoculation, spores are microscopic so if you can see it, thats alot
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
Edited by cronicr (06/04/13 02:54 AM)
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Jman420
MagicJ



Registered: 12/23/12
Posts: 866
Loc: On a Cloud
Last seen: 4 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18365938 - 06/04/13 02:12 AM (11 years, 7 months ago) |
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Welcome!
This thread is hilarious rofl
-------------------- --My Everything You Need To Know Tek--
Disregard the haters and "know it alls", it is everything you need to know TO GET STARTED.
If you want to become an expert, you'll need to do a lot more research.
-----------------------------------------------------------------------
Open Your Mind To A New Future
-----------------------------------------------------------------------
    
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Stropharis



Registered: 04/02/07
Posts: 1,854
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Re: Growing from a print... Have a few Questions. [Re: Jman420]
#18366270 - 06/04/13 06:09 AM (11 years, 7 months ago) |
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Stro's Agar Prep
By the time you get this ready, I should have a pictorial on spore to clean culture using agar complete.
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: Stropharis]
#18366466 - 06/04/13 07:45 AM (11 years, 7 months ago) |
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you know how they do it in cali kids goin dumb thizzin' n shit
haha but in all seriousness folks, sdankys advice doesn't seem too far off too me - that plus agar work, couldn't you clean up your culture and have it free of contaminants and you'd be good to go?
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18366704 - 06/04/13 09:24 AM (11 years, 7 months ago) |
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Quote:
VirginiaGentleman said:
I just put some Cinct spores on agar yesterday, if you're interested i can PM some pics when they grow out so you can know what you're looking for
sorry...couldn't help myself
roughly translates into "Clear your inbox honey, I'm gonna send you so many dick pics my junk will have sunburn from the camera flash" 

Quote:
TheBoomking said:
haha but in all seriousness folks, sdankys advice doesn't seem too far off too me - that plus agar work, couldn't you clean up your culture and have it free of contaminants and you'd be good to go?
It's feasible, but not sustainable
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Just prepped my agar with Potato Dextrose (Yeast-extract) Agar (PD(Y)A)... Plus I went out yesterday to my horse stables, and found a few fresh samples to clone. I didn't feel to confident about how old those prints were. Plus I think the old "BLACK" prints were just wishful thinking they're kind of like a black brown. I figured it was better safe than sorry, the new prints are BLACK I put a dot of sharpie next to them and it matches!!(: Yay. So time to start.
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18367450 - 06/04/13 12:43 PM (11 years, 7 months ago) |
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could have used a fruit instead of spores
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18367470 - 06/04/13 12:48 PM (11 years, 7 months ago) |
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I can still do that, I still have the mushrooms...
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18367472 - 06/04/13 12:48 PM (11 years, 7 months ago) |
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awesome!
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18367500 - 06/04/13 12:56 PM (11 years, 7 months ago) |
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Should I use the base of the stem, or the gills??? And how long do I let the agar cool before pouring into the dishes? Then how long do I wait to actually place the spores/fruit in the petrie dish? Sorry thats the part I'm super confused on...
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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Stropharis



Registered: 04/02/07
Posts: 1,854
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18367558 - 06/04/13 01:12 PM (11 years, 7 months ago) |
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Quote:
riley.123 said: Should I use the base of the stem, or the gills??? And how long do I let the agar cool before pouring into the dishes? Then how long do I wait to actually place the spores/fruit in the petrie dish? Sorry thats the part I'm super confused on...
I don't have a write up for cloning yet, but frank has one.
Frank's simple cloning tek
Your last two questions are answered in the write up I previously recommended.
Quote:
Stropharis said: Stro's Agar Prep By the time you get this ready, I should have a pictorial on spore to clean culture using agar complete.
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: Stropharis]
#18367670 - 06/04/13 01:40 PM (11 years, 7 months ago) |
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Done!(:
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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agar turns solid at like 105 F keep it just above to pour and your good, when its solid its pretty much good to go \\
to clone take a tissue sample closest to the bottom of the stem, I would think if you tried to clone from the gills you'd risk germinating spores at the same time kind've sabotaging your clone 
Quote:
thevirginiagentlemen said:
It's feasible, but not sustainable
how's it not sustainable?
Edited by TheBoomking (06/05/13 10:06 PM)
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18375275 - 06/05/13 10:20 PM (11 years, 7 months ago) |
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Quote:
TheBoomking said:
how's it not sustainable?
Make a syringe from a wild contaminated print use only a few drops from syringe for agar and return to storage bacteria continues to reproduce while in storage now you have a shit syringe full of bacteria
Not saying it's not possible, just that there's methods for better results
you're better off just scraping spores onto agar in a SAB
for short term results it doesn't really matter though
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/05/13 10:21 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18375276 - 06/05/13 10:21 PM (11 years, 7 months ago) |
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Misunderstood
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18375303 - 06/05/13 10:26 PM (11 years, 7 months ago) |
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yeah you can clean them up, but the syringe is likely to go to shit making it useless in a few weeks
it's all good brah
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/05/13 10:27 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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I hate syringes so as soon as I realized you were discouraging their use I'm like "why am I disagreeing with this?"
Really all that's holding me back from PE is my distrust of anything in a liquid suspension and swabs are hard to find 
I'm a bit drunk to boot
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18375367 - 06/05/13 10:39 PM (11 years, 7 months ago) |
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after pissing away 2 syringes i made from some giant mutant fruits, i'll never look back.
I finally got results dropping the veil remnants of one of those fruits onto agar, and now it looks like i may loose that grain spawn to bacteria
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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hahah yeah good point, why make the syringe when you can just scrape/wipe into agar 
What do you mean swabs are hard to find? I only work with pe...
Edited by TheBoomking (06/06/13 08:59 AM)
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18376895 - 06/06/13 09:07 AM (11 years, 7 months ago) |
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Quote:
Pastywhyte said: Really all that's holding me back from PE is my distrust of anything in a liquid suspension and swabs are hard to find 
And swabs usually don't get handled/sterilized/dried properly...every single swab I've gotten from someone else has been covered in bacteria. However, the few I've made myself worked like a champ 
I don't like spore syringes either but if you want a PE syringe, go get one from a sponsor, it's totally worth it
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Growing from a print... Have a few Questions. [Re: FrankHorrigan]
#18377019 - 06/06/13 09:39 AM (11 years, 7 months ago) |
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Im really tempted but, I seem to have rotten luck with syringes even from sponsors of all the syringes I've gotten from sponsors, only one worked, most grew nothing and I had a one beautiful specimen of aspergillus. Really most of my successful grows have been from prints acquired from the good people in the marketplace 
Maybe i will take a chance and try once more for some PE or APE before i retire
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18377045 - 06/06/13 09:47 AM (11 years, 7 months ago) |
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wow sorry to hear about your bad luck
yeah I highly recommend getting ape
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18377172 - 06/06/13 10:25 AM (11 years, 7 months ago) |
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It sounds like a bad thing but I'm not bitter or nothing. If anything my poor luck with syringes only made me determined to learn to use agar and grains. Who knows I might have been content to dick around with PF tek a lot longer otherwise. So from that point of view it was probably a good thing in the long run.
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MattyMushies
[_CLOSET NERD_]

Registered: 05/27/13
Posts: 90
Loc: Paradise
Last seen: 10 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18377178 - 06/06/13 10:31 AM (11 years, 7 months ago) |
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I started using agar heavily not too long ago for cloning and don't plan to turn back. It's simply too damn easy. I feel bad for the dozen LCs that will probably never have a use.
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: MattyMushies]
#18377239 - 06/06/13 10:50 AM (11 years, 7 months ago) |
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the day when you discover agar...
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18379649 - 06/06/13 07:29 PM (11 years, 7 months ago) |
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I think I just started to get the tiniest little bit of growth on a couple plates! White fuzz, Yay!(: hahaha I really hope they don't get contaminated, what are some good ways I can prevent this??? Also, should I be putting water in these? They seem dry.....
Thanks for all of your guys' help!!!(: Couldn't do it with out you <3
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18379661 - 06/06/13 07:32 PM (11 years, 7 months ago) |
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thats the beauty of agar,see a contam and transfer away
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18379691 - 06/06/13 07:38 PM (11 years, 7 months ago) |
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I totally forgot about that, the beauty in transfer! haha And how hydrated do I keep the plates??? Like should I be putting a couple drops of water on them???
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18379699 - 06/06/13 07:41 PM (11 years, 7 months ago) |
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No the agar will provide the water. Excess water is an invitation for bacteria. That's why when using spore syringe to agar you only use a single drop and try to not let it roll around on the plate.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18379708 - 06/06/13 07:42 PM (11 years, 7 months ago) |
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Quote:
cronicr said: thats the beauty of agar,see a contam and transfer away
and culture slants
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: twistedty]
#18379844 - 06/06/13 08:11 PM (11 years, 7 months ago) |
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Has anyone on this thread documented growing with agar, and has *good* pictures of what it should look like at different stages, or know of a different thread I can find those at... I want to make sure everything looks right
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 4 years, 5 months
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18379946 - 06/06/13 08:30 PM (11 years, 7 months ago) |
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i only have slants in the fridge atm
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: twistedty]
#18380622 - 06/06/13 10:30 PM (11 years, 7 months ago) |
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see the top left? that's contamination, what you would want to transfer is healthy mycelial growth as seen at the other locations - but let it grow out at least 5-10 days before transferring to new media. You can continue to transfer as long as you like, but once you have clean growth it's ready for grains or your choice of proper media.
Pan. Cinct. introduced 6-3

Now go post pics in The Girls of Shroomery Thread
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/06/13 10:38 PM)
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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Edited by TheBoomking (06/07/13 08:40 AM)
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18385349 - 06/07/13 09:36 PM (11 years, 7 months ago) |
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VERY healthy growth on one plate! YAY. Fairly good growth on a second, the others don't really seem to be taking. I varied the parts of the mushroom I used, Spores, base, gill ect. The two plates using the base of the mushroom are DEFINITELY better!!! I am soooooo excited... now how long do I let this grow before transferring to either a grain or bulk substrate of wood chips...? I can do either, any advice on this decision as well??? I'm kind of looking for quantity over quality, for the first time anyways :P ... I just want to grow them already!!! PS: No contams on these two plates are seen, should I still do a transfer???
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18385454 - 06/07/13 09:55 PM (11 years, 7 months ago) |
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Quote:
riley.123 said: or bulk substrate of wood chips...?
I'm kind of looking for quantity over quality, for the first time anyways :P ... I just want to grow them already!!!
The failure is strong with this one 
Do Cali schools teach reading anymore?
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/07/13 09:57 PM)
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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post pics
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18385504 - 06/07/13 10:07 PM (11 years, 7 months ago) |
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Quote:
cronicr said: post pics

BTW they're doing great, i may isolate for a good while to really narrow down a genome
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/07/13 10:09 PM)
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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?????
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Quote:
VirginiaGentleman said:
Quote:
riley.123 said: or bulk substrate of wood chips...?
I'm kind of looking for quantity over quality, for the first time anyways :P ... I just want to grow them already!!!
The failure is strong with this one 
Do Cali schools teach reading anymore?
What do you mean by that???
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18387100 - 06/08/13 09:49 AM (11 years, 7 months ago) |
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Cinct will not grow from wood.
You should be focusing on quality and comprehension of what you're trying to accomplish, that isn't shining through right now
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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Re: Growing from a print... Have a few Questions. [Re: FrankHorrigan]
#18387415 - 06/08/13 11:29 AM (11 years, 7 months ago) |
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if you picked the cincs you should know what they grow on, use that
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18387723 - 06/08/13 01:01 PM (11 years, 7 months ago) |
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I found them on Grass???? I just didn't think simply grass would be a substantial substrate... I'm sorry, I've read many threads about transferring mycelia to wood chips, and that just came out as an example... I know they like stable-like areas, would it work if I transfer to grass with aged/ partially broken down horse manure with some hay or something like that??? I would just prefer to grow out side if possible...
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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newera
Stranger


Registered: 03/05/13
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18387732 - 06/08/13 01:04 PM (11 years, 7 months ago) |
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pan cincs love manure, i had some pop up in my pile of h-poo this year!
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 50 seconds
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Re: Growing from a print... Have a few Questions. [Re: newera]
#18387806 - 06/08/13 01:29 PM (11 years, 7 months ago) |
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for outdoors a mix of h-poo and grass clippings would be great!i'd start piling up the spots right now and turning the pile every day till it's dry and aged, you don't want green grass clippings in there. i would love to see some pics of your dishes
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18389186 - 06/08/13 07:09 PM (11 years, 7 months ago) |
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Quote:
cronicr said: for outdoors a mix of h-poo and grass clippings would be great!i'd start piling up the spots right now and turning the pile every day till it's dry and aged, you don't want green grass clippings in there. i would love to see some pics of your dishes
I think I'll start that today!(: and I just transferred like an hour ago, so I'll post pics tomorrow evening...
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18398985 - 06/10/13 07:33 PM (11 years, 7 months ago) |
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Its all destroyed!!! God damned contaminations! Screw thissssss. Hahaha I swear, I'm going to cry. Mushrooms are the so important in my life! But, idk how long I can afford to keep buying $20 an 1/8. I've never been so excited about anything in my whole lifeeee, now what do I have? A total failure... I transferred and then left them for a day, and the agar plates dried out! Why do the mushroom Gods despise me?!?! Will someone please tell me a BEGINNER method I can use... My biggest problem is no one will deliver spores to Cali... And I think I'm using contaminated prints. I dont know what I can do, I want to just buy everything I need and do it 100% "by the book" so to speak, for my first time... buttttt whats the point of spending that money if I'm not even gonna be growing anything thats worth it you know? haha Does anyone know of anywhere that will ship to Cali???
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18399990 - 06/10/13 10:05 PM (11 years, 7 months ago) |
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http://www.mushroomvideos.com/Download
http://www.mushroomvideos.com/Strain-Isolation
you should watch the agar section (maybe all of it and even buy it - I did ) . the musics a little rough but That will help you learn how to "clean up" a spore print. basically once you see growth you transfer the growrth to a new clean plate - isolating it from contams. sometimes you only need to do it once, sometimes three, somtimes you don't need to at all.
I'm sure some kind gentlemen of the shroomery will be willing to send some cubensis prints, to an attractive young cali girl like yourself.
(cubensis are a more beginner friendly strain)
ask around if you haven't already been pm'd
some might demand some sort of favors in return though - they're usually from Virginia
Edited by TheBoomking (06/10/13 11:33 PM)
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18400177 - 06/10/13 10:34 PM (11 years, 7 months ago) |
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Quote:
VirginiaGentleman said: The failure is strong with this one 
Quote:
riley.123 said: Its all destroyed!!!
CALLED IT!
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18400225 - 06/10/13 10:40 PM (11 years, 7 months ago) |
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Quote:
TheBoomking said:
I'm sure some kind gentlemen of the shroomery will be willing to send some cubensis prints, to an attractive young cali girl like yourself.
some might demand some sort of favors in return though - they're usually from Virginia though 
Tit's for prints
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 4 years, 5 months
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Quote:
VirginiaGentleman said:
Quote:
TheBoomking said:
I'm sure some kind gentlemen of the shroomery will be willing to send some cubensis prints, to an attractive young cali girl like yourself.
some might demand some sort of favors in return though - they're usually from Virginia though 
Tit's for prints 
i would do that also. and 5 pre sterilized dishes
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: twistedty]
#18400269 - 06/10/13 10:46 PM (11 years, 7 months ago) |
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Let the bidding begin!
Prints + 5 dishes + ???
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Quote:
VirginiaGentleman said:
Quote:
VirginiaGentleman said: The failure is strong with this one 
Quote:
riley.123 said: Its all destroyed!!!
CALLED IT!

Well congratulations? Thanks for being mean about it... That was most definitely necessary in the situation.
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18400321 - 06/10/13 10:54 PM (11 years, 7 months ago) |
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Quote:
riley.123 said:
Well congratulations? Thanks for being mean about it... That was most definitely necessary in the situation.

Your success is solely based on your will to understand and proper implementation, so right back at ya darling
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/10/13 10:55 PM)
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: twistedty]
#18400395 - 06/10/13 11:10 PM (11 years, 7 months ago) |
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Quote:
twistedty said:
Quote:
VirginiaGentleman said:
Quote:
TheBoomking said:
I'm sure some kind gentlemen of the shroomery will be willing to send some cubensis prints, to an attractive young cali girl like yourself.
some might demand some sort of favors in return though - they're usually from Virginia though 
Tit's for prints 
i would do that also. and 5 pre sterilized dishes
Oh wow! How generous!!!  Well you see, this is all almost too kind coming from all of you folk, I mean I don't even know you! Wait....... I don't even know you? .......... Oh yeah. However; I do have to thank you all for the so very generous offers I have received this evening
Unfortunately, I have to decline... You know, I may not fit the normal mushroom hunters profile, but that doesn't mean I'm some fucking retarded air head either. I really didn't wish to be made fun of when I created my account here... Just because you don't want to help me, or I seem inexperienced doesn't mean I want demeaning Jokes thrown at me on my posts. ( Not trying to be a bitch, but it simply seems a little immature to me... As if I'd solicit my self for any other purpose than crack <<< THat was a joke by the way.......
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Quote:
VirginiaGentleman said:
Quote:
riley.123 said:
Well congratulations? Thanks for being mean about it... That was most definitely necessary in the situation.

Your success is solely based on your will to understand and proper implementation, so right back at ya darling
Isn't having the proper items to do it with a contributing factor??? Unfortunately, it just isn't really possible for me to do a lot of mushroom cultivation related buying right now... I just wanted to give it a shot with what I had. Uhm My bad...? Oh wait no it isn't...
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18400444 - 06/10/13 11:24 PM (11 years, 7 months ago) |
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Absolutely. Unfortunately for you, you're not willing or able to gather the necessities; and as such have a much higher failure rate. You took that risk, and you reaped the rewards.
 GTFO
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: twistedty]
#18400455 - 06/10/13 11:25 PM (11 years, 7 months ago) |
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Quote:
TheBoomking said:
I'm sure some kind gentlemen of the shroomery will be willing to send some cubensis prints, to an attractive young cali girl like yourself.
some might demand some sort of favors in return though - they're usually from Virginia though 
Why Thank you(: ... But damn, I'm not so sure about some of the "gentlemen" on shroomery anymore. hahahaha. Seriously though I'd pay for some prints if someone wanted to sell them to me! I would be overly happy(: haha
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18400484 - 06/10/13 11:31 PM (11 years, 7 months ago) |
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Quote:
riley.123 said: Its all destroyed!!! God damned contaminations! Screw thissssss. Hahaha I swear, I'm going to cry. Mushrooms are the so important in my life! But, idk how long I can afford to keep buying $20 an 1/8. I've never been so excited about anything in my whole lifeeee, now what do I have? A total failure... I transferred and then left them for a day, and the agar plates dried out! Why do the mushroom Gods despise me?!?! Will someone please tell me a BEGINNER method I can use...
They teach agar work to high school students nowadays, so it doesn't really get any more noob-friendly. And seriously, don't fret. You have several things going for you: you live in a mountain of h-poo with pans growing all around you. Any mistreatment you receive here that's not due to the internet being the internet is probably jealousy based. You know how many people here would kill for that setup? Virginia Gentleman is one of them. Seriously, don't give him your address.
You wasted thirty cents worth of agar on those plates. This is not a life-ruining failure. Get on your horse and ride around until you see more clonable specimens. Make like ten more plates at the same time, and do it with more water this time. You're shooting for 2-3% agar by weight here. That's 3-4.5 grams per ten dishes and 150mL water.
If you want to splurge, go get some fish antibiotics for like ten bucks at the pet store. Tetracycline is broad-spectrum and will definitely help with bacterial contamination. If not, just transfer away from contams onto plates you already know to be good (because you were able to grow your clone on one made in the same batch).
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Quote:
VirginiaGentleman said: Absolutely. Unfortunately for you, you're not willing or able to gather the necessities; and as such have a much higher failure rate. You took that risk, and you reaped the rewards.
 GTFO
Yeah, not able to... and all I was doing was asking for a recommended tek thats easy for beginners, and I asked for a few tips on how to prevent this from happening again... You decided to say I told you so... (real "mature"?????) and Make a joke about bidding me off. WTF. That really is not all that funny...
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: Psilicon]
#18400519 - 06/10/13 11:39 PM (11 years, 7 months ago) |
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Quote:
van der griegen said:
Quote:
riley.123 said: Its all destroyed!!! God damned contaminations! Screw thissssss. Hahaha I swear, I'm going to cry. Mushrooms are the so important in my life! But, idk how long I can afford to keep buying $20 an 1/8. I've never been so excited about anything in my whole lifeeee, now what do I have? A total failure... I transferred and then left them for a day, and the agar plates dried out! Why do the mushroom Gods despise me?!?! Will someone please tell me a BEGINNER method I can use...
They teach agar work to high school students nowadays, so it doesn't really get any more noob-friendly. And seriously, don't fret. You have several things going for you: you live in a mountain of h-poo with pans growing all around you. Any mistreatment you receive here that's not due to the internet being the internet is probably jealousy based. You know how many people here would kill for that setup? Virginia Gentleman is one of them. Seriously, don't give him your address.
You wasted thirty cents worth of agar on those plates. This is not a life-ruining failure. Get on your horse and ride around until you see more clonable specimens. Make like ten more plates at the same time, and do it with more water this time. You're shooting for 2-3% agar by weight here. That's 3-4.5 grams per ten dishes and 150mL water.
If you want to splurge, go get some fish antibiotics for like ten bucks at the pet store. Tetracycline is broad-spectrum and will definitely help with bacterial contamination. If not, just transfer away from contams onto plates you already know to be good (because you were able to grow your clone on one made in the same batch).
Wow. Thank you very much... THIS was helpful(: haha I'll be going on a ride early in the morning to go hunting, and hopefully I'll get a couple actives, and can make a positive ID on them, so I can start again... Maybe a little better prepared this time... or A LOT(: hahaha
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18400528 - 06/10/13 11:41 PM (11 years, 7 months ago) |
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Quote:
van der griegen said: Any mistreatment you receive here that's not due to the internet being the internet is probably jealousy based. You know how many people here would kill for that setup? Virginia Gentleman is one of them. Seriously, don't give him your address.
Yeah, real jealous here 
Quote:
riley.123 said:
Yeah, not able to... and all I was doing was asking for a recommended tek thats easy for beginners, and I asked for a few tips on how to prevent this from happening again... You decided to say I told you so... (real "mature"?????) and Make a joke about bidding me off. WTF. That really is not all that funny...
It's a basic idea of mixxing and sterilizing agar, then introducing samples and waiting
wash rinse and repeat until you have clean growth
not many people are willing to help if you do not have the incentive to learn on your own. it's simple
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/10/13 11:41 PM)
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
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Re: Growing from a print... Have a few Questions. [Re: Psilicon]
#18400539 - 06/10/13 11:43 PM (11 years, 7 months ago) |
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Oh, and I forgot to mention the lowest-tech of all teks: dump a bucket of water on an active outdoor mycelium.
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: Psilicon]
#18400561 - 06/10/13 11:48 PM (11 years, 7 months ago) |
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Don't forget to do all your transfers In a still air box or in front of a flow hood!
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Mooby
Stranger



Registered: 11/25/12
Posts: 130
Loc: The West
Last seen: 3 years, 11 months
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18400849 - 06/11/13 12:43 AM (11 years, 7 months ago) |
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Whoa guys, take it easy. Shroomery is supposed to be about community and encouragement (and it usually is), but seeing disparaging threads like this makes me question whether or not that's the case...Messing up sucks bad enough without being insulted for it.
That being said, don't get discouraged. Failures are the best experience if you can tough it out.
I know you can't just order spores, and that sucks. I'd personally do everything you can do make Franks Cloning Tek work if somebody doesn't come through with a syringe or print. If you can get some clean myc on agar, there is no reason that growing it out to finished boomers should be too much trouble. Read and read through everything FrankHorrigan has put together, and then read it again while you put it into action. He has done a stellar job of putting together reliable methods. The best shortcut is to follow the teks exactly,as any substitution or straying will welcome problems. If you read Frank teks closely enough, you won't even have to ask another question to successfully grow. He's seriously put together a step-by-step guide for growing start to finish. I like tmethyl and Notahacker420's tek collections as well. If you are really careful, you can seriously just follow along without knowing much theory; you'll certainly gather a fair amount just by doing EXACTLY what they say to do. Oh yeah, you will get mushrooms too!
Best of luck!
-------------------- Subvert the dominant paradigm; pee in the sink!
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 4 years, 5 months
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Re: Growing from a print... Have a few Questions. [Re: Mooby]
#18401441 - 06/11/13 04:20 AM (11 years, 7 months ago) |
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i was just joking im a jokester. pm me
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: Mooby]
#18401944 - 06/11/13 08:33 AM (11 years, 7 months ago) |
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Quote:
Mooby said: Whoa guys, take it easy. Shroomery is supposed to be about community and encouragement (and it usually is), but seeing disparaging threads like this makes me question whether or not that's the case...Messing up sucks bad enough without being insulted for it.
That being said, don't get discouraged. Failures are the best experience if you can tough it out.
I know you can't just order spores, and that sucks. I'd personally do everything you can do make Franks Cloning Tek work if somebody doesn't come through with a syringe or print. If you can get some clean myc on agar, there is no reason that growing it out to finished boomers should be too much trouble. Read and read through everything FrankHorrigan has put together, and then read it again while you put it into action. He has done a stellar job of putting together reliable methods. The best shortcut is to follow the teks exactly,as any substitution or straying will welcome problems. If you read Frank teks closely enough, you won't even have to ask another question to successfully grow. He's seriously put together a step-by-step guide for growing start to finish. I like tmethyl and Notahacker420's tek collections as well. If you are really careful, you can seriously just follow along without knowing much theory; you'll certainly gather a fair amount just by doing EXACTLY what they say to do. Oh yeah, you will get mushrooms too!
Best of luck!

Awesome!(: I'm going on a ride right now to TRY to find something to clone... You know, since Southern California is teaming with actives!!! not....... But I'll definitely bookmark a couple of those teks! Thank you so much<3
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: twistedty]
#18401952 - 06/11/13 08:35 AM (11 years, 7 months ago) |
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Quote:
twistedty said: i was just joking im a jokester. pm me
^^^ Me or Mooby??? And Mhhm suuuuuure you are (; hahahaha
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18403844 - 06/11/13 03:50 PM (11 years, 7 months ago) |
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Am I the only one that cracks up every time they read this whole post???
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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Stropharis



Registered: 04/02/07
Posts: 1,854
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Re: Growing from a print... Have a few Questions. [Re: Stropharis]
#18431075 - 06/17/13 07:59 AM (11 years, 6 months ago) |
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Quote:
Stropharis said: Stro's Agar Prep
By the time you get this ready, I should have a pictorial on spore to clean culture using agar complete.
Stro's Cleaning and Isolating On Agar
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