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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: Stropharis]
#18366466 - 06/04/13 07:45 AM (11 years, 7 months ago) |
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you know how they do it in cali kids goin dumb thizzin' n shit
haha but in all seriousness folks, sdankys advice doesn't seem too far off too me - that plus agar work, couldn't you clean up your culture and have it free of contaminants and you'd be good to go?
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18366704 - 06/04/13 09:24 AM (11 years, 7 months ago) |
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Quote:
VirginiaGentleman said:
I just put some Cinct spores on agar yesterday, if you're interested i can PM some pics when they grow out so you can know what you're looking for
sorry...couldn't help myself
roughly translates into "Clear your inbox honey, I'm gonna send you so many dick pics my junk will have sunburn from the camera flash" 

Quote:
TheBoomking said:
haha but in all seriousness folks, sdankys advice doesn't seem too far off too me - that plus agar work, couldn't you clean up your culture and have it free of contaminants and you'd be good to go?
It's feasible, but not sustainable
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Just prepped my agar with Potato Dextrose (Yeast-extract) Agar (PD(Y)A)... Plus I went out yesterday to my horse stables, and found a few fresh samples to clone. I didn't feel to confident about how old those prints were. Plus I think the old "BLACK" prints were just wishful thinking they're kind of like a black brown. I figured it was better safe than sorry, the new prints are BLACK I put a dot of sharpie next to them and it matches!!(: Yay. So time to start.
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 38 seconds
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18367450 - 06/04/13 12:43 PM (11 years, 7 months ago) |
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could have used a fruit instead of spores
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18367470 - 06/04/13 12:48 PM (11 years, 7 months ago) |
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I can still do that, I still have the mushrooms...
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 29 minutes, 38 seconds
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18367472 - 06/04/13 12:48 PM (11 years, 7 months ago) |
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awesome!
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: cronicr]
#18367500 - 06/04/13 12:56 PM (11 years, 7 months ago) |
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Should I use the base of the stem, or the gills??? And how long do I let the agar cool before pouring into the dishes? Then how long do I wait to actually place the spores/fruit in the petrie dish? Sorry thats the part I'm super confused on...
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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Stropharis



Registered: 04/02/07
Posts: 1,854
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Re: Growing from a print... Have a few Questions. [Re: riley.123]
#18367558 - 06/04/13 01:12 PM (11 years, 7 months ago) |
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Quote:
riley.123 said: Should I use the base of the stem, or the gills??? And how long do I let the agar cool before pouring into the dishes? Then how long do I wait to actually place the spores/fruit in the petrie dish? Sorry thats the part I'm super confused on...
I don't have a write up for cloning yet, but frank has one.
Frank's simple cloning tek
Your last two questions are answered in the write up I previously recommended.
Quote:
Stropharis said: Stro's Agar Prep By the time you get this ready, I should have a pictorial on spore to clean culture using agar complete.
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riley.123
I fucking LOVE this website



Registered: 01/21/12
Posts: 102
Loc: Canyon Lake, Californinia
Last seen: 5 years, 9 months
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Re: Growing from a print... Have a few Questions. [Re: Stropharis]
#18367670 - 06/04/13 01:40 PM (11 years, 7 months ago) |
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Done!(:
-------------------- Theres acid rain in my brain and its killing the flowers in my heart...
I tried making sense of it, and then I realized I should be trying to make sense as to why I was even trying to make sense...
All I know is that I'd rather take a pile of physically harmless mushrooms every couple of months to bring me back to nature and the universe, than to chug a large cup of coffee every morning just to have the energy and will to go to school/work.
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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agar turns solid at like 105 F keep it just above to pour and your good, when its solid its pretty much good to go \\
to clone take a tissue sample closest to the bottom of the stem, I would think if you tried to clone from the gills you'd risk germinating spores at the same time kind've sabotaging your clone 
Quote:
thevirginiagentlemen said:
It's feasible, but not sustainable
how's it not sustainable?
Edited by TheBoomking (06/05/13 10:06 PM)
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18375275 - 06/05/13 10:20 PM (11 years, 7 months ago) |
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Quote:
TheBoomking said:
how's it not sustainable?
Make a syringe from a wild contaminated print use only a few drops from syringe for agar and return to storage bacteria continues to reproduce while in storage now you have a shit syringe full of bacteria
Not saying it's not possible, just that there's methods for better results
you're better off just scraping spores onto agar in a SAB
for short term results it doesn't really matter though
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/05/13 10:21 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18375276 - 06/05/13 10:21 PM (11 years, 7 months ago) |
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Misunderstood
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18375303 - 06/05/13 10:26 PM (11 years, 7 months ago) |
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yeah you can clean them up, but the syringe is likely to go to shit making it useless in a few weeks
it's all good brah
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
Edited by VirginiaGentleman (06/05/13 10:27 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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I hate syringes so as soon as I realized you were discouraging their use I'm like "why am I disagreeing with this?"
Really all that's holding me back from PE is my distrust of anything in a liquid suspension and swabs are hard to find 
I'm a bit drunk to boot
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VirginiaGentleman
Lignin seeker



Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18375367 - 06/05/13 10:39 PM (11 years, 7 months ago) |
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after pissing away 2 syringes i made from some giant mutant fruits, i'll never look back.
I finally got results dropping the veil remnants of one of those fruits onto agar, and now it looks like i may loose that grain spawn to bacteria
-------------------- Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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hahah yeah good point, why make the syringe when you can just scrape/wipe into agar 
What do you mean swabs are hard to find? I only work with pe...
Edited by TheBoomking (06/06/13 08:59 AM)
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18376895 - 06/06/13 09:07 AM (11 years, 7 months ago) |
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Quote:
Pastywhyte said: Really all that's holding me back from PE is my distrust of anything in a liquid suspension and swabs are hard to find 
And swabs usually don't get handled/sterilized/dried properly...every single swab I've gotten from someone else has been covered in bacteria. However, the few I've made myself worked like a champ 
I don't like spore syringes either but if you want a PE syringe, go get one from a sponsor, it's totally worth it
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Growing from a print... Have a few Questions. [Re: FrankHorrigan]
#18377019 - 06/06/13 09:39 AM (11 years, 7 months ago) |
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Im really tempted but, I seem to have rotten luck with syringes even from sponsors of all the syringes I've gotten from sponsors, only one worked, most grew nothing and I had a one beautiful specimen of aspergillus. Really most of my successful grows have been from prints acquired from the good people in the marketplace 
Maybe i will take a chance and try once more for some PE or APE before i retire
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TheBoomking
I really am Bret McKenzie



Registered: 01/21/11
Posts: 836
Last seen: 5 years, 8 months
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Re: Growing from a print... Have a few Questions. [Re: Pastywhyte]
#18377045 - 06/06/13 09:47 AM (11 years, 7 months ago) |
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wow sorry to hear about your bad luck
yeah I highly recommend getting ape
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Growing from a print... Have a few Questions. [Re: TheBoomking]
#18377172 - 06/06/13 10:25 AM (11 years, 7 months ago) |
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It sounds like a bad thing but I'm not bitter or nothing. If anything my poor luck with syringes only made me determined to learn to use agar and grains. Who knows I might have been content to dick around with PF tek a lot longer otherwise. So from that point of view it was probably a good thing in the long run.
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