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 DanKnugget
Mysterious Traveller
Registered: 07/15/03
Posts: 1,074
Loc: On a boat
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 Tyvek on PF jars. 
#1826147 - 08/18/03 12:41 PM (20 years, 7 months ago) |
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I have some pf jars, and innoculated 4 days ago. I used tyvek filters on the lids(1 layer only). I have yet to see any growth. I am probably just being impatient, but will tyvek give enough air for a pf jar. BTW, i didn't use a top verm barrier, due to lack of spare verm. help me please! 
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My 3 proverbs:
1. School is for people who don't know stuff.
2. Jobs are for losers.
3. Reading is for people with nothing better to do.
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soochi
Chef
Registered: 08/13/02
Posts: 2,420
Loc: The Richest County
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 Re: Tyvek on PF jars. [Re: DanKnugget]
#1826247 - 08/18/03 12:58 PM (20 years, 7 months ago) |
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4 days is nothing, write back in two weeks.
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Wee, sleeket, cowran, tim'rous beastie,
O, what panic's in thy breastie!
Thou need na start awa sae hasty,
Wi' bickering brattle!
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Diploid
Cuban
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
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Re: Tyvek on PF jars. [Re: DanKnugget]
#1826251 - 08/18/03 12:59 PM (20 years, 7 months ago) |
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Quote:
I have some pf jars, and innoculated 4 days ago. I used tyvek filters on the lids(1 layer only). I have yet to see any growth. I am probably just being impatient, but will tyvek give enough air for a pf jar. BTW, i didn't use a top verm barrier, due to lack of spare verm. help me please!
OK, a few of things...
You say you used tyvek and no top verm layer. How did you cover the holes in the lid and tyvek after you inoculated? Or did you just leave the holes open. Tyvek will effectively block contamination, but if you leave the needle hole through which you inoculated open, a vector can sneak in.
Did you shake the syringe before inoculating? How much solution did you use and at how many inoculation points?
It can easily take a week for growth to show up and if your temperature is much below 85F, it can take even longer.
Finally, it's INOCULATION, with one 'N'. Get that straight! 
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Republican Values:
1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.
4) We need a smaller, less-intrusive government.
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 MrSleep
P. Cubensis
Registered: 04/05/03
Posts: 804
Last seen: 19 years, 5 months
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Re: Tyvek on PF jars. [Re: Diploid]
#1826370 - 08/18/03 01:22 PM (20 years, 7 months ago) |
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Danknugget that's more than enough air exchange. I wouldn't be surprised if your jars dry out actually. Through trial and error my friend it's a waste of time, in his experience. He has a bunch of tyvek but just uses tinfoil now with no air exchange during colonization, still gets cakes done in 2 weeks time.
-Mr.Sleep
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Ravinoff
LethargicSnorting Duck
Registered: 07/15/03
Posts: 686
Last seen: 20 years, 7 months
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Re: Tyvek on PF jars. [Re: MrSleep]
#1826451 - 08/18/03 01:34 PM (20 years, 7 months ago) |
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I've no growth on my pint jars for 2 weeks now and I use tyvek 
I think I got me some bunk syringes
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Diploid
Cuban
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
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Re: Tyvek on PF jars. [Re: Ravinoff]
#1826460 - 08/18/03 01:36 PM (20 years, 7 months ago) |
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Either that or your jars dried out due to the tyvek.
-Diploid
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Republican Values:
1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.
4) We need a smaller, less-intrusive government.
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deathinchaos
regular
Registered: 12/22/02
Posts: 174
Last seen: 13 years, 1 month
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 Re: Tyvek on PF jars. [Re: Diploid]
#1827156 - 08/18/03 04:29 PM (20 years, 7 months ago) |
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so would you guys say tyvek is abad idea...and if you used it should you replace it with electric tape again
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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DanKnugget
Mysterious Traveller
Registered: 07/15/03
Posts: 1,074
Loc: On a boat
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Re: Tyvek on PF jars. [Re: LiL_KuSsH]
#1828047 - 08/18/03 09:05 PM (20 years, 7 months ago) |
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i used tyvek on pf and popcorn, and i did not inoculate thru the tyvek, slightly lifted it under an oven sucker vent, and I think the popcorn looks pretty dry, so i squirted some sterile water in one of the popcorn jars, to see if it would help. i put that jar in my computer case to incubate it, and left the other ones in tupperware near a sunny window, it should get a bit hotter there. Thanks for the help!
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My 3 proverbs:
1. School is for people who don't know stuff.
2. Jobs are for losers.
3. Reading is for people with nothing better to do.
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DanKnugget
Mysterious Traveller
Registered: 07/15/03
Posts: 1,074
Loc: On a boat
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Re: Tyvek on PF jars. [Re: DanKnugget]
#1833746 - 08/20/03 01:56 PM (20 years, 7 months ago) |
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i got growth on the popcorn, finally. about time!
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My 3 proverbs:
1. School is for people who don't know stuff.
2. Jobs are for losers.
3. Reading is for people with nothing better to do.
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