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OfflineMythmaker
I'm a teapot

Registered: 05/21/13
Posts: 90
Last seen: 16 days, 21 hours
A FIRST-TIMER's GROW LOG - using mycobag and B+ spores
    #18300691 - 05/21/13 08:56 PM (11 years, 7 months ago)

Hi all. I've lurked this forum for quite some time and read many tutorials and teks, etc. And now I am making the venture into the world of ethnomycology by starting my first grow.

So, since I'm a noob, I figured I'd start small and simple by starting with some mycobags (from REDACTED), some spores (from REDACTED), and fruiting in-vitro. Sorry if this method makes some of you veterans cringe ... but like I said, this is my first time and I really want to do something super simple before I refine my methods and make LC's, rye jars, and a fruiting chamber. I'll do that stuff later.

Anyways, I inoculated my bag on the 16th of May. I was SUPER careful not to risk contamination (room with stagnant air, gloves, alcohol on the needle and injection site, flamed needle).

BUT, the one thing I was unsure about was this: Of course, before inoculating, I kneaded the substrate in the bag to loosen it up. But immediately after inoculating, I kneaded again because this tutorial told me to.

So my question is:
1.) Was it a good idea to knead immediately after inoculation?
2.) If I DIDN'T knead immediately afterwards, and just let the spores settle against the side of the bag, would I see growth already? (after 5 days)


Thanks ahead of time for your expert advice! I'll be sure to update this post with pics as the mycelia make progress. Which they hopefully will.

EDIT:

14 DAYS AFTER INOCULATION:
Quote:

Mythmaker said:
The mycelia has started making a comeback after its first mash. Now it's in lots of little clumps resembling used kitty litter!



Should I shake the bag so that these balls get buried? Or just leave them alone and let nature do her thang? (My logic: More contact with the substrate instead of just air.)




15 DAYS AFTER INOCULATION:
Quote:

Mythmaker said:
The balls of mycelia are starting to create strands between each other and join together!









21 DAYS AFTER INOCULATION:
Quote:

Mythmaker said:
21 days after inoc. I'd estimate about 75% colonized. I plan on mashing it tomorrow.








Edited by Mythmaker (06/06/13 09:53 AM)

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InvisibleMykes logos
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18300792 - 05/21/13 09:12 PM (11 years, 7 months ago)

1st off, welcome to the shroomery:thumbup:. 2nd, please remove sponsors' names from your OP as that's against the rules..

Quote:

Mythmaker said:
... but like I said, this is my first time and I really want to do something super simple before I refine my methods and make LC's, rye jars, and a fruiting chamber. I'll do that stuff later.




Hmmmm. Starting with a SGFC and PF cakes isn't a bad idea either... but I understand your thought process.

Quote:

Mythmaker said:
I was SUPER careful not to risk contamination (room with stagnant air, gloves, alcohol on the needle and injection site, flamed needle).




I hope you flame sterilized the needle AFTER you alcohol sanitized it...

Never do that again. Flame sterilize only...

Quote:

Mythmaker said:
BUT, the one thing I was unsure about was this: Of course, before inoculating, I kneaded the substrate in the bag to loosen it up. But immediately after inoculating, I kneaded again because this tutorial told me to.

So my question is:
1.) Was it a good idea to knead immediately after inoculation?
2.) If I DIDN'T knead immediately afterwards, and just let the spores settle against the side of the bag, would I see growth already? (after 5 days)




That's fine, it'll work no worries. Many people prefer to keep the spores in one place so its easier for plasmogamy to occur. What was the injection port made out of? Did you use RTV silicone or did it come with one or something?

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OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 90
Last seen: 16 days, 21 hours
Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mykes logos]
    #18302225 - 05/22/13 04:17 AM (11 years, 7 months ago)

Quote:

Mykes logos said:
1st off, welcome to the shroomery:thumbup:. 2nd, please remove sponsors' names from your OP as that's against the rules..





Oh! My bad. Sorry.

Quote:

Mykes logos said:
Quote:

Mythmaker said:
I was SUPER careful not to risk contamination (room with stagnant air, gloves, alcohol on the needle and injection site, flamed needle).




I hope you flame sterilized the needle AFTER you alcohol sanitized it...

Never do that again. Flame sterilize only...





Well, I swabbed it with alcohol, THEN flamed it (until it was glowing). Is that bad? I mean I figured that combining the two couldn't hurt. Then again I'm a newbie so please correct me. :p

Quote:

Mykes logos said:
Quote:

Mythmaker said:
BUT, the one thing I was unsure about was this: Of course, before inoculating, I kneaded the substrate in the bag to loosen it up. But immediately after inoculating, I kneaded again because this tutorial told me to.

So my question is:
1.) Was it a good idea to knead immediately after inoculation?
2.) If I DIDN'T knead immediately afterwards, and just let the spores settle against the side of the bag, would I see growth already? (after 5 days)




That's fine, it'll work no worries. Many people prefer to keep the spores in one place so its easier for plasmogamy to occur. What was the injection port made out of? Did you use RTV silicone or did it come with one or something?




Ahh okay. Thanks for the reassurance!

And I'm not sure what the port is made of. And yes it came with the bag. I think it's neoprene or something. Or yeah, maybe silicone.


Anyways, thanks for the quick response! I think I'm gonna like it here.


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InvisibleFooMan
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18302311 - 05/22/13 05:03 AM (11 years, 7 months ago)

That "tutorial" you linked to wasn't really a tutorial at all. It was someone stating their plans based on steps they found somewhere in the forums that "looked the best" to them.

At any rate, I wouldn't have kneaded the bag after inoculation. It's better to wait until you have a sizeable amount of growth before mixing up the substrate and spreading the mycelium around. Otherwise your spores may start germinating in areas of the bag you don't see. Leave the bag alone from this point and let it do its thing.


--------------------

Quick WBS Prep

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Offlineveda_sticks
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18302317 - 05/22/13 05:06 AM (11 years, 7 months ago)

Quote:

Anyways, I inoculated my bag on the 16th (5 days ago at the time of this post). I was SUPER careful not to risk contamination (room with stagnant air, gloves, alcohol on the needle and injection site, flamed needle).




Open air inoculations are never recomended, some people get away with it and some have terrible success rates with open air.And sometimes the people that "get away" with it suddenly get hit by contams.

If you want high success rates, build a SAB (still air box) and work with simple sanatary and sterile procedures. its much easier to prevent contaminant spores and microbes getting into jars and bags within a smaller confined space than it is in a much larger room. Not to mention that you isolate your projects from yourself since only your hands and arms and hands are in the box, which ofcoarse you have gloves on and long sleaves to so that your not shedding dead skin cells into the air.

Anyway, to your question. Generally its best not to massage the bag as it can bury the spores inside the substrate, so you might not see it when it starts growing until a little later when it starts poking out from the substrate.

its not a problem but it just might be a concern when nothing has been happening for a while and you think its dud but its not.

Grains are easy, The actuall prep work amounts to usually 30 minutes of actual work, the rest is just wwaiting for grains to soak, then simmer, dry and ofcoarse pressure cooking.

Ive just moved to grains (after a fair amount of pf tek experience) and although some of my jars were not the best (some mushy grains) most of them worked out.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 90
Last seen: 16 days, 21 hours
Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: veda_sticks]
    #18303133 - 05/22/13 10:47 AM (11 years, 7 months ago)

Quote:

FooMan said:
At any rate, I wouldn't have kneaded the bag after inoculation. It's better to wait until you have a sizeable amount of growth before mixing up the substrate and spreading the mycelium around. Otherwise your spores may start germinating in areas of the bag you don't see. Leave the bag alone from this point and let it do its thing.




Quote:

veda_sticks said:
Anyway, to your question. Generally its best not to massage the bag as it can bury the spores inside the substrate, so you might not see it when it starts growing until a little later when it starts poking out from the substrate.

its not a problem but it just might be a concern when nothing has been happening for a while and you think its dud but its not.






Ah. Got it. Thanks, guys.

So basically, I'll just have to be patient and wait for colonization.


'Notha question:
Do the mycelial strands "meet up" and join together? Is this plasmogamy? How long before I'll see this occur on the outside of the substrate (if most of the spores are mixed somewhere in the middle)?


--------------------

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InvisibleMykes logos
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18306816 - 05/22/13 10:39 PM (11 years, 7 months ago)


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OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 90
Last seen: 16 days, 21 hours
Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mykes logos]
    #18314863 - 05/24/13 02:57 PM (11 years, 7 months ago)

Quote:

Mykes logos said:
While you're waiting, spend $10, support a loved shroomery moderator, and learn more:cool::thumbup:



Thanks! I'll definitely check it out. Btw are those videos mainly for cultivating food mushrooms? If so, would it make any difference?

Quote:

Mykes logos said:
Patience is everything.




I'll say! I'm at 8 days after inoculation and I'm already seeing some white clumps scattered throughout the bag. :grin:


--------------------

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OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 90
Last seen: 16 days, 21 hours
Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18319155 - 05/25/13 02:45 PM (11 years, 7 months ago)

Woohoo! At approx 20-25% colonization 9 days after inoc. :heart:


--------------------

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InvisibleMykes logos
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18322583 - 05/26/13 10:47 AM (11 years, 7 months ago)

Quote:

Mythmaker said:
Thanks! I'll definitely check it out. Btw are those videos mainly for cultivating food mushrooms? If so, would it make any difference?





The same general processes are used for pretty much every type of mushroom... I don't grow P. cubensis, but from what I've read/heard they are probably the easiest mushroom species to grow (besides oyster species), especially if you're using pre-sterilized bags and spore syringes... and there's plenty of info on here about that species.

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OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 90
Last seen: 16 days, 21 hours
Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mykes logos]
    #18327024 - 05/27/13 08:38 AM (11 years, 7 months ago)

How do I know if my bag is dehydrated?


It's been at about 20-25% colonization for the past few days. Am I just impatient and paranoid? I think yes .... :p


--------------------

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OfflineCoreyRubble
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18327162 - 05/27/13 09:33 AM (11 years, 7 months ago)

If it is at 25% then shake it/break it up and watch it zoom

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OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 90
Last seen: 16 days, 21 hours
Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: CoreyRubble]
    #18327411 - 05/27/13 11:01 AM (11 years, 7 months ago)

Quote:

CoreyRubble said:
If it is at 25% then shake it/break it up and watch it zoom



I've heard to wait til 30-40% to do that, but okay.


Also, should I give the bag some light or keep it in the dark until it's time to fruit?


--------------------

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OfflineCoreyRubble
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18327498 - 05/27/13 11:27 AM (11 years, 7 months ago)

Really doesn't matter, I keep mine in the dark.

Mycellium benefits from light at all stages though, So light is good.

Make sure it is indirect light though.

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OfflineMythmaker
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Registered: 05/21/13
Posts: 90
Last seen: 16 days, 21 hours
Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: CoreyRubble]
    #18327562 - 05/27/13 11:45 AM (11 years, 7 months ago)

Quote:

CoreyRubble said:
Really doesn't matter, I keep mine in the dark.

Mycellium benefits from light at all stages though, So light is good.

Make sure it is indirect light though.



So like, placing the bag near a lamp in my bedroom? Would that be indirect?

Just to be clear: Your definition of "direct" light would be sunlight or bright lights for growing pot, right? :p


--------------------

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OfflinecronicrMFacebook
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18327907 - 05/27/13 12:53 PM (11 years, 7 months ago)

just put it on a shelf some where while it's colonizing, if you can see it thats good enough, when fruiting time comes give it direct intense lighting


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineCoreyRubble
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: cronicr]
    #18328020 - 05/27/13 01:21 PM (11 years, 7 months ago)

:whathesaid:

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OfflineMythmaker
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: CoreyRubble]
    #18328043 - 05/27/13 01:25 PM (11 years, 7 months ago)

Got it! I've also heard about dropping the temperature when they're ready to fruit ... Is this necessary (like changing to 12/12 lighting when pot is ready to make bud) or is it just an extra little push?


--------------------

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OfflinecronicrMFacebook
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: Mythmaker]
    #18328058 - 05/27/13 01:28 PM (11 years, 7 months ago)

no need to do that with cubes


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinePussyFart
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Re: FIRST-TIMER HERE - using mycobag and B+ spores, have a few Q's [Re: cronicr]
    #18328070 - 05/27/13 01:30 PM (11 years, 7 months ago)

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

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