|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
illuminati
Strange


Registered: 06/17/07
Posts: 556
Loc: Aboard the T.A.R.D.I.S.
Last seen: 3 years, 8 months
|
Question About Agar Media
#18311426 - 05/23/13 08:29 PM (10 years, 11 months ago) |
|
|
I'm of the understanding that cultures should only be kept on one type of agar media for so long, and then it should be transferred to another media to keep it viable. I'm curious as to how many different types of media one would need in order to keep a culture viable indefinitely. For example, if a culture is started on Media "A" and moved to Media "B" after a period of time, how many other different agar recipes should be used before Media "A" is suitable to use again?
Thanks for any help
-------------------- I didn't get turned on I just got turned I wasn't as aroused as I was concerned for each one of 'em I've hurt and every time I've been burned I've got a lot to teach but even more to learn
|
Satanschild
goodbye

Registered: 01/16/13
Posts: 281
|
Re: Question About Agar Media [Re: illuminati]
#18317909 - 05/25/13 09:01 AM (10 years, 11 months ago) |
|
|
Keep in mind genetics also degrade when transfering. I dont think a different kind of agar preparation will make a difference.
What you might be looking for: http://www.mushroomvideos.com/Master-Culture-Slants
Quote:
To avoid senescence, experienced mushroom growers store their cultures under refrigeration, which greatly slows down the process of cell division, prolonging the life of the organism. The process of making master culture slants and storing mycelium that is demonstrated here has proved to be capable of storing mushroom tissue for at least two years without further work. This is accomplished by placing a small piece of hardwood into each test tube along with the agar medium and mushroom mycelium.
The mushroom mycelium colonizes the nutrient soaked hardwood, and can then become dormant and last for at least 2 years. I have several cultures that have been in storage this way for nearly ten years and they are still viable. Each year, I take out a small piece of mycelium to test. If it grows well, it’s then used to inoculate grains to start the process of growing mushrooms. The original master culture slant is then placed back into refrigeration.
If you choose not to use wood in your culture slants, I'd suggest making transfers at least twice per year to avoid losing your cultures. When making transfers from culture slants, cut a small piece of mycelium and transfer it to a Petri dish. Allow it to grow out approximately 1 cm(unless contamination appears sooner), and then transfer from the leading edge of the growth to a new Petri dish. Once the mycelium on the second dish grows out 1cm, if no contamination has become evident, place a tiny piece of mycelium from the leading edge into a fresh test tube slant for further storage. The remaining mycelium on the Petri dish can then be used to inoculate grains for mushroom growing.
Edited by Satanschild (05/25/13 09:04 AM)
|
illuminati
Strange


Registered: 06/17/07
Posts: 556
Loc: Aboard the T.A.R.D.I.S.
Last seen: 3 years, 8 months
|
Re: Question About Agar Media [Re: Satanschild]
#18318056 - 05/25/13 09:58 AM (10 years, 11 months ago) |
|
|
Thank you, that actually makes a lot of sense; I don't know why I was under the impression it would be possible to just keep transferring a culture in order to keep it alive forever.
Would there be any benefit to changing the recipe for the agar media when taking a sample from a master slant? For example, if the slant was made using MEA, would it benefit the mycellium in any way to be grown on another agar media (prior to being used to inoculate a grain jar), such as PDA?
-------------------- I didn't get turned on I just got turned I wasn't as aroused as I was concerned for each one of 'em I've hurt and every time I've been burned I've got a lot to teach but even more to learn
|
Satanschild
goodbye

Registered: 01/16/13
Posts: 281
|
Re: Question About Agar Media [Re: illuminati]
#18318685 - 05/25/13 12:49 PM (10 years, 11 months ago) |
|
|
I doubt genetics when transfering could ever vary enough to make a big difference, even weaker mycelium will still grow on either petri dishes.
If you're really trying to select the strongest genetics you would have to incorporate the ingredient you're spawning to in the agar mixture and leave out the dextrose. And like mushroomvideos said transfer the leading edge to a new plate. But I assume this will only affect the growth rate of the mycelium, so the effects are neglectable.
|
|