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Offlinethundahoof
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Metabolite City (UPDATE: 6/8/13)
    #18304139 - 05/22/13 02:01 PM (11 years, 7 months ago)


MS rye 1 qt spawned to straight coir 2 qt and like 2-3 tbsp coffee ground.  Does this likely have some bacteria present?  Also, I understand fruiting is still possible.  Could I just cover this with bw and fruit?  My rh is super high right now, gulf coast.


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Edited by thundahoof (06/08/13 05:36 PM)

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InvisibleTranscendingLife
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Re: Metabolite City [Re: thundahoof]
    #18304179 - 05/22/13 02:09 PM (11 years, 7 months ago)

Looks like bacteria to me.


--------------------
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OfflinecronicrMFacebook
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Re: Metabolite City [Re: TranscendingLife]
    #18304183 - 05/22/13 02:10 PM (11 years, 7 months ago)

:whathesaid:that first pic tells all:frown:


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InvisibleFrankHorrigan
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Re: Metabolite City [Re: thundahoof]
    #18304186 - 05/22/13 02:11 PM (11 years, 7 months ago)

Don't cover the tub with bubble wrap...really bad idea for a mono, we've been over this :crazy2:

Esp with your high room RH, there is zero reason to bubble wrap or wax paper the surface.  In a mono you should always be trying to increase FAE.

That substrate is fucked. Especially with the coffee in there, you certainly have a bacterial problem running rampant that's going to get moldy when you fruit it.

If you're looking to try anyways, you want to maximize your FAE to try to beat back the bacteria. The failure rate is pretty damn high though. With the added coffee that tub doesn't stand a chance :frown:


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OfflinecronicrMFacebook
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Re: Metabolite City [Re: FrankHorrigan]
    #18304200 - 05/22/13 02:13 PM (11 years, 7 months ago)

i've only ever managed one flush at best with bacteria problems like that and to top it off alot of the fruits were infected and gross


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Invisiblefastfred
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Re: Metabolite City [Re: FrankHorrigan]
    #18304213 - 05/22/13 02:15 PM (11 years, 7 months ago)

This is just metabolites.  It happens in sterile culture all the time. (I should actually say occasionally)

You can verify if you want by taking a sample and looking through it.  If it's bacterially contaminated it will be cloudy, otherwise it will be clear.

On pic 5 you can see clearly right through some of the drops, so it's not bacterial.

Usually this happens from too much heat or not enough air.  Kind of odd to see it in a monotub like this.


-FF


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It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

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OfflineTmethylM
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Re: Metabolite City [Re: fastfred]
    #18304261 - 05/22/13 02:23 PM (11 years, 7 months ago)

Quote:

fastfred said:
This is just metabolites.  It happens in sterile culture all the time. (I should actually say occasionally)

You can verify if you want by taking a sample and looking through it.  If it's bacterially contaminated it will be cloudy, otherwise it will be clear.

On pic 5 you can see clearly right through some of the drops, so it's not bacterial.

Usually this happens from too much heat or not enough air.  Kind of odd to see it in a monotub like this.


-FF



Agreed, but I think there is some active bacteria in this substrate due to the very stringy single strand rhizo.
But I would put money on this tub fruiting just fine, any takers?:hehehe:
I would fruit this in 1-2 days.

Props to OP for not calling it myc piss.


--------------------
¯\_(ツ)_/¯

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Offlinethundahoof
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Re: Metabolite City [Re: fastfred]
    #18304268 - 05/22/13 02:24 PM (11 years, 7 months ago)

Allright, I think this was from a suspect jar that I didn't want to transfer anyway.  So no big loss here.  It's not quite a monotub, just a tray, but my sgfc had something fall on it and it busted it to pieces.  I just remember seeing someone that just covered with bubble wrap and fruited in open air. 

Gah that first pic is awfully oozey.
Quote:

fastfred said:
This is just metabolites.  It happens in sterile culture all the time. (I should actually say occasionally)

You can verify if you want by taking a sample and looking through it.  If it's bacterially contaminated it will be cloudy, otherwise it will be clear.

On pic 5 you can see clearly right through some of the drops, so it's not bacterial.

Usually this happens from too much heat or not enough air.  Kind of odd to see it in a monotub like this.


-FF



I forgot to add it does get hot in this room too, like high 80's in the day sometimes.  I will try to take a look in the scope thanks.


--------------------

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OfflinecronicrMFacebook
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Re: Metabolite City [Re: Tmethyl]
    #18304271 - 05/22/13 02:24 PM (11 years, 7 months ago)

Quote:

Tmethyl said:
Quote:

fastfred said:
This is just metabolites.  It happens in sterile culture all the time. (I should actually say occasionally)

You can verify if you want by taking a sample and looking through it.  If it's bacterially contaminated it will be cloudy, otherwise it will be clear.

On pic 5 you can see clearly right through some of the drops, so it's not bacterial.

Usually this happens from too much heat or not enough air.  Kind of odd to see it in a monotub like this.


-FF



Agreed, but I think there is sonlme active bacteria in this substrate due to the very stringy single strand rhizo.
But I would put money on this tub fruiting just fine, any takers?:hehehe:



i'll put a print up on one flush


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleFrankHorrigan
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Re: Metabolite City [Re: cronicr]
    #18304284 - 05/22/13 02:27 PM (11 years, 7 months ago)

Quote:

cronicr said:
Quote:

Tmethyl said:
Quote:

fastfred said:
This is just metabolites.  It happens in sterile culture all the time. (I should actually say occasionally)

You can verify if you want by taking a sample and looking through it.  If it's bacterially contaminated it will be cloudy, otherwise it will be clear.

On pic 5 you can see clearly right through some of the drops, so it's not bacterial.

Usually this happens from too much heat or not enough air.  Kind of odd to see it in a monotub like this.


-FF



Agreed, but I think there is sonlme active bacteria in this substrate due to the very stringy single strand rhizo.
But I would put money on this tub fruiting just fine, any takers?:hehehe:



i'll put a print up on one flush




Two prints against it. 

My opinion: No chance of a flush. Bacteria-laden coffee present.

Even if it fully colonizes, i bet it's just going to get hot and develop some wet bubble. (this is not my bet however- I am betting against a successful flush for whatever reason :crazy2: )

We doing this thunda?


--------------------

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Frank's tips and tricks. Updated on 3/21/14
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Edited by FrankHorrigan (05/22/13 02:34 PM)

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OfflineTmethylM
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Re: Metabolite City [Re: FrankHorrigan]
    #18304296 - 05/22/13 02:30 PM (11 years, 7 months ago)

5 prints on 2+ healthy flushes.


--------------------
¯\_(ツ)_/¯

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InvisibleFrankHorrigan
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Re: Metabolite City [Re: Tmethyl]
    #18304305 - 05/22/13 02:31 PM (11 years, 7 months ago)

:lmafo:


I'll :pm: you both my address in advance :smirk:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflinecronicrMFacebook
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Re: Metabolite City [Re: Tmethyl]
    #18304307 - 05/22/13 02:31 PM (11 years, 7 months ago)

oooh this is getting exciting:bearbreakdance: there should be a gamble your prints thread somewhere lol


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It doesn't matter what i think of you...all that matters is clean spawn
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OfflinePussyFart
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Re: Metabolite City [Re: FrankHorrigan]
    #18304314 - 05/22/13 02:32 PM (11 years, 7 months ago)

Quote:

FrankHorrigan said:
:lmafo:

I'll :pm: you both my address in advance :smirk:



:laugh2::goodluck:

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InvisibleTranscendingLife
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Re: Metabolite City [Re: cronicr] * 1
    #18304330 - 05/22/13 02:35 PM (11 years, 7 months ago)

Quote:

cronicr said:
there should be a gamble your prints thread somewhere lol






--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
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:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
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InvisibleFrankHorrigan
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Re: Metabolite City [Re: TranscendingLife]
    #18304335 - 05/22/13 02:36 PM (11 years, 7 months ago)

:whathesaid:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflinecronicrMFacebook
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Re: Metabolite City [Re: FrankHorrigan]
    #18304362 - 05/22/13 02:43 PM (11 years, 7 months ago)

i think i got a pan print with your name on it, it's labeled unknown from laos and at the top it says to: TL. There were 2 of them but i sent one out to frank already lol just saying that i feel like i just prepaid the guy :lmafo:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinethundahoof
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Re: Metabolite City [Re: thundahoof]
    #18304364 - 05/22/13 02:44 PM (11 years, 7 months ago)

Upon further inspection at 60-100x a droplet doesn't appear cloudy but looks to have some brownish specks.


I don't really have anything else going on, waiting on jars and hoping those masters were safe after seeing this colonize.  So no go on just bw the top?  I don't have a lid for this tray but I could still rig up minimono I suppose.


--------------------

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OfflineTmethylM
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Re: Metabolite City [Re: FrankHorrigan]
    #18304384 - 05/22/13 02:46 PM (11 years, 7 months ago)

:ohsodevious:
Go mini mono Thunda.
Fruit at this time tomorrow.


--------------------
¯\_(ツ)_/¯

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InvisibleTranscendingLife
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Re: Metabolite City [Re: cronicr]
    #18304388 - 05/22/13 02:48 PM (11 years, 7 months ago)

Quote:

cronicr said:
i think i got a pan print with your name on it, it's labeled unknown from laos and at the top it says to: TL. There were 2 of them but i sent one out to frank already lol just saying that i feel like i just prepaid the guy :lmafo:




:shrug2: Never asked for one.
I can get prints any time, though. So thanks, but no thanks.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
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