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OfflineHostileX420
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Liquid Culture into PF Tek is it possible ......
    #18300082 - 05/21/13 06:54 PM (10 years, 8 months ago)

LC into PF Tek: is it possible once I have myc in my LC to inoculate on BRF? And is it true this will give me a jump on other contaims? :pipesmoke:


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Offlinefungitarian
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Re: Liquid Culture into PF Tek is it possible ...... [Re: HostileX420]
    #18300112 - 05/21/13 07:01 PM (10 years, 8 months ago)

LCs are extremely unreliable. theres no way to tell if the culture has any contams until you actually grow it out to test it. so you may waste a month +/- of time you could spend doing agar strain isolation or just a standard ms grow.


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OfflinePoorSpore
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Re: Liquid Culture into PF Tek is it possible ...... [Re: HostileX420]
    #18300127 - 05/21/13 07:04 PM (10 years, 8 months ago)

I am wondering same thing! Have a jar of honey water with GT and looking for best route until I get a pressure cooker.Hoping to have luck with a dozen brf cakes.Good luck to you and hopefully someone with more experience than me has so me good advice and  can point us to a good tek.


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Offlinefungitarian
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Re: Liquid Culture into PF Tek is it possible ...... [Re: PoorSpore]
    #18300145 - 05/21/13 07:07 PM (10 years, 8 months ago)

no one with a lot of experience is going to recommend an LC.


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OfflinePoorSpore
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Re: Liquid Culture into PF Tek is it possible ...... [Re: PoorSpore]
    #18300153 - 05/21/13 07:09 PM (10 years, 8 months ago)

I have read that it is unreliable, but I knocked an odd number of jars last week and had 10cc or so left in syringe so decided to try LC rather than toss it


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OfflinecronicrFacebook
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Re: Liquid Culture into PF Tek is it possible ...... [Re: PoorSpore]
    #18300176 - 05/21/13 07:12 PM (10 years, 8 months ago)

always run a test jar before going all out


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Offlinefungitarian
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Re: Liquid Culture into PF Tek is it possible ...... [Re: PoorSpore]
    #18300188 - 05/21/13 07:14 PM (10 years, 8 months ago)

best of luck in the world to you. i never said it wont work. its just not efficient to wait to see if your LC actually works through the whole process without contams..

LCs have and can work, there are just much better ways to produce alot of innoculant but with an isolated strain that will perform per your preferences


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OfflineHostileX420
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Re: Liquid Culture into PF Tek is it possible ...... [Re: fungitarian]
    #18300202 - 05/21/13 07:16 PM (10 years, 8 months ago)

what is the shelf life if you succeed in actually doing it right and all go's well?


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OfflineHostileX420
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Re: Liquid Culture into PF Tek is it possible ...... [Re: cronicr]
    #18300215 - 05/21/13 07:18 PM (10 years, 8 months ago)

HEY BRO!!! Yeah i was going to do test runs this time instead of losing 36 jars again lol for sure!!! :headbang:  :headbang:  :rockon:  :rockon:


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Offlinefungitarian
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Re: Liquid Culture into PF Tek is it possible ...... [Re: HostileX420]
    #18300230 - 05/21/13 07:22 PM (10 years, 8 months ago)

in fridge you can keep it for a long time. and you can always make more ( using the LC you like just make it the same way as before but instead of spores use your LC)


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Edited by fungitarian (05/21/13 07:23 PM)


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OfflineHostileX420
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Re: Liquid Culture into PF Tek is it possible ...... [Re: fungitarian]
    #18300257 - 05/21/13 07:26 PM (10 years, 8 months ago)

O Yeah your right i just knock up anoter jar with that LC right DUH!!!:facepalm3: But how long is long a couple months i won't even try years


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Offlinefungitarian
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Re: Liquid Culture into PF Tek is it possible ...... [Re: HostileX420]
    #18300267 - 05/21/13 07:28 PM (10 years, 8 months ago)

when i didnt know that LCs werent optimal i had success with 6month old LC at least. just keep em fridgerated.

if you're trying to keep a constant grow going after you decide you like the LC, I would make a new culture every 3-4months or so.


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OfflineHostileX420
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Re: Liquid Culture into PF Tek is it possible ...... [Re: fungitarian]
    #18300348 - 05/21/13 07:46 PM (10 years, 8 months ago)

:headbang:thanks man i'll remember that! and i already have PD i just need some agar then i can clone i would prefer that above all else unless there is a better way to make Cultures!:pipesmoke:


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Edited by HostileX420 (05/21/13 07:55 PM)


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Offlinefungitarian
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Re: Liquid Culture into PF Tek is it possible ...... [Re: HostileX420]
    #18300529 - 05/21/13 08:24 PM (10 years, 8 months ago)

Look up how to isolate. :thumbup:


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OfflineHostileX420
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Re: Liquid Culture into PF Tek is it possible ...... [Re: fungitarian]
    #18300558 - 05/21/13 08:30 PM (10 years, 8 months ago)

Ahhh your talking about selecting diff isomorphic sections on a PD yea i no about that i think its risomorphic sections anyways the long stringy sections that reach out am i right?


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InvisibleFrankHorrigan
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Re: Liquid Culture into PF Tek is it possible ...... [Re: HostileX420]
    #18300662 - 05/21/13 08:49 PM (10 years, 8 months ago)

Quote:

HostileX420 said:
And is it true this will give me a jump on other contaims?




I just wanted to pop in and mention that there is no "jump" or "head start" on contams that your myc will win. You want to use good sterile procedure so there is no contaminants present in the first place.

If you feel like you need to "outrace" contams, you've already lost the battle :frown:


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OfflineHostileX420
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Re: Liquid Culture into PF Tek is it possible ...... [Re: FrankHorrigan]
    #18301059 - 05/21/13 09:51 PM (10 years, 8 months ago)

:syringe:Thanks sterile procedure is deff 1st on my list! And i no i no i no:facepalm3:I was reading about it in the forums and it struck my interest Aaah what a ya gonna do :shrug: It was worth a shot!!!


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