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Offlinemycofever
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Storing pleurotus ostreatus
    #18253971 - 05/12/13 05:38 PM (11 years, 9 days ago)

I have some slants made up of pleurotus ostreatus. I had them stored in the fridge with all of my other cultures. This one however, Never stopped growing.It has filled the whole top up with mycelium. How can I slow it down any help will be appreciated.


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OfflineJeff
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Re: Storing pleurotus ostreatus [Re: mycofever]
    #18254065 - 05/12/13 05:58 PM (11 years, 9 days ago)

I don't think you can slow it down short of freezing it.  Freezing has adverse affects on agar though.  Pleurtus myc is friggin amazing.


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Offlinemycofever
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Re: Storing pleurotus ostreatus [Re: Jeff]
    #18254226 - 05/12/13 06:35 PM (11 years, 9 days ago)

I was hoping that you would not tell me that.:smile: Oh well I guess my plans on keeping that strain for a long time might not happen then. The only reason that I like this one so much is because I found them in the wild and cloned them. It's got sentimental value.:lol:


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Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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OfflineJeff
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Re: Storing pleurotus ostreatus [Re: mycofever]
    #18254373 - 05/12/13 07:11 PM (11 years, 9 days ago)

Just do a shorter cycles on your transfers.  If it something you like it is worth it.


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Invisiblelaughingsol
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Re: Storing pleurotus ostreatus [Re: mycofever]
    #18254382 - 05/12/13 07:14 PM (11 years, 9 days ago)

You could try another storage method- sterile distilled water, or maybe frozen sawdust spawn.  I haven't tried keeping sawdust spawn frozen for long term storage, but I've kept pleurotus frozen on sawdust for around 6 months with no loss of vigor.  Seeing as how it can live through just about anything, I wouldn't be surprised if you could take a little bit of frozen sawdust spawn back to agar after a year or two of storage.


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Offlinemycofever
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Re: Storing pleurotus ostreatus [Re: Jeff]
    #18254404 - 05/12/13 07:21 PM (11 years, 9 days ago)

Quote:

Jeff said:
Just do a shorter cycles on your transfers.  If it something you like it is worth it.



I may try this. Maybe on petri dishes and don't let it reach a stopping point.


Also the distilled water method may work I have never done this before but it would stop it with no nutrition. I have seen the tek. It appears to be promising.


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Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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OfflineOICU812
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Re: Storing pleurotus ostreatus [Re: mycofever]
    #18254803 - 05/12/13 08:42 PM (11 years, 9 days ago)

Quote:

mycofever said:
Quote:

Jeff said:
Just do a shorter cycles on your transfers.  If it something you like it is worth it.



I may try this. Maybe on petri dishes and don't let it reach a stopping point.


Also the distilled water method may work I have never done this before but it would stop it with no nutrition. I have seen the tek. It appears to be promising.




Try non-nutrient agar.  Agar + water but nothing else, except maybe a little salt.  AKA water agar or starving agar.  You might find a few other ideas in FastFred's Media Cookbook

The distilled water method sounds most promising, though.  Don't forget to sterilize it before use.


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Edited by OICU812 (05/12/13 08:44 PM)

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Offlinemycofever
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Re: Storing pleurotus ostreatus [Re: OICU812]
    #18254857 - 05/12/13 08:54 PM (11 years, 9 days ago)

:nodofunderstanding:

I will give this a shot. distilled water storage


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Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

Edited by mycofever (05/12/13 09:01 PM)

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Offlineforrest
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Re: Storing pleurotus ostreatus [Re: mycofever]
    #18255269 - 05/12/13 10:28 PM (11 years, 9 days ago)

what about drying spawn? i read about it on this forum, is it not really acknowledged?


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Offlinemycofever
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Re: Storing pleurotus ostreatus [Re: forrest]
    #18256397 - 05/13/13 05:26 AM (11 years, 8 days ago)

I am not just trying to keep the mycelium stored. I also want to keep it from ageing It takes so much energy for it to colonize a bag of grain spawn or a jar.. Grains Is not a good method for this.


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Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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Offlineforrest
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Re: Storing pleurotus ostreatus [Re: mycofever]
    #18257312 - 05/13/13 11:45 AM (11 years, 8 days ago)

i also came across a topic of someone who revived a dried up agar plate...


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Invisiblefastfred
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Re: Storing pleurotus ostreatus [Re: forrest]
    #18257742 - 05/13/13 01:33 PM (11 years, 8 days ago)

I've heard of freezing and drying.  But your best and most reliable methods would be reducing the carbon source (sugars) in your agar.  You might try something around 0.5% or less.  Water agar would also be worth trying.  If you use tap water for that it should have enough minerals to keep things going.

You could also try an oil overlay.  Just add mineral oil over your slants or cultures.  This prevents air from getting to them and stops/slows their respiration.  You will want to combine this with water agar or low nutrient concentrations since it's best to slow them down before choking them out.

Freezing is probably the best option, but you'll want to check their viability and tune your methods.  A common method is to use a powdered milk solution as cryoprotectant.  Glycerin and/or propylene glycol are also commonly used.  I've also heard of using sugar solutions.

Give things a go and let us know what works the best for you.  It's too bad we haven't already worked this out to a better extent.  Most likely any of these techniques will work just fine.


-FF


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Offlinemycofever
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Re: Storing pleurotus ostreatus [Re: fastfred]
    #18258150 - 05/13/13 02:52 PM (11 years, 8 days ago)

Thanks fastfred you had a lot of good info in there.


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Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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Invisible36fuckin5
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Re: Storing pleurotus ostreatus [Re: mycofever]
    #18310887 - 05/23/13 06:54 PM (10 years, 11 months ago)

You could just make up a LC with a wedge. Once it eats up the sugar or whatever in the LC, it really has nowhere else to go.


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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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