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Offliner05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
Last seen: 18 years, 10 months
ectomycorrhizal media
    #1812830 - 08/14/03 08:38 AM (20 years, 6 months ago)

Hey, does anyone have on hand agar recipies for ectomycorrhizal basidiomycetes?


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Offliner05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
Last seen: 18 years, 10 months
Re: ectomycorrhizal media [Re: r05c03]
    #1814421 - 08/14/03 04:58 PM (20 years, 6 months ago)

More specifically I am looking for a recipe to MNC agar. The only place I have found it is a journal article from 1995 published in japanese. Not a big help.


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OfflineMycena
mycoexplorer
Registered: 05/02/03
Posts: 270
Loc: indonesia
Last seen: 11 years, 9 months
Re: ectomycorrhizal media [Re: r05c03]
    #1819599 - 08/16/03 11:26 AM (20 years, 6 months ago)

Can you elaborate on what the M , N and C stand for?

Ive got an article somewhere on recipes foor boletus sp. but i am NOt looking for it - if you saw the pile youd understand - though i am on the lookout for it sio ill post if i find it OK

Ive subbed Suillus onto straight PDA BTW - it grows Veeeery slowly


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Offliner05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
Last seen: 18 years, 10 months
Re: ectomycorrhizal media [Re: Mycena]
    #1822192 - 08/17/03 06:28 AM (20 years, 6 months ago)

Yeah sorry, I do not have that info, but I do not think that the letters stand for particular ingredients but rather some guy's name. Any ectomycorrhizal recipies you have would be appreciated. Thanks


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Offlinezeronio
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Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 years, 5 months
Re: ectomycorrhizal media [Re: r05c03]
    #1823150 - 08/17/03 03:00 PM (20 years, 6 months ago)

All I have is BAF medium:

BAF Medium
Glucose 30.0 g
Peptone 2.0 g
Yeast extract 0.2 g
KH2PO4 0.5 g
MgSO4 x 7 H2O 0.5 g
FeCl3 x 6 H2O 10.0 mg
ZnSO4 x 7 H2O 1.0 mg
MnSO4 5.0 mg
CaCl2 x 2 H2O 100.0 mg
Thiamin HCl 50.0 ?g
Biotin 1.0 ?g
Folic acid 100.0 ?g
Inositol 50.0 ?g
Agar 15.0 g
Distilled water 1000.0 ml
pH 5.8 - 6.3.

Maybe you'll find something useful here:
http://www.mykopat.slu.se/mycorrhiza/kantarellfiler/texter/nitrogen.pdf


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Offliner05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
Last seen: 18 years, 10 months
Re: ectomycorrhizal media [Re: zeronio]
    #1823313 - 08/17/03 03:49 PM (20 years, 6 months ago)

Thank you good sir!



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