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OfflineNewtimer
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4 Weeks popcorn no PC and it went great 100% colonization
    #18221992 - 05/06/13 11:40 AM (11 years, 8 months ago)

Hello, as you Can already tell from my name on here im new at this, my first grow experience. I inoculated 24 jars pint-size jars six days ago along with three 1 gallon Ziploc bags half full of substrate and one two pint  jar.  I inoculated them with four different strains with spore syringes include creeper, B , Ecuador and Cambodian  now it's been six days and I see no mycelium growth which makes me nervous, i also see no contamination factors either. I'm using yellow popcorn which I purchased from grocery store. These are the steps I have taken thus far. I soaked Popcorn for 15 hours, rinsed and boiled for hour and half. Then I washed all jars and boiled jars and tops for hour. After that I placed jars in micro wave for 3 min just to be sure zero contamination. Rinsed my popcorn a final time and let dry on towel for two hours until mostly dry "it felt nice and soft but not mushy and not very wet" I then filled jars with popcorn and do not have a pressure cooker so instead I used a huge roaster in which I placed all the jars in covered and boiled i did same with bags just zipped the bags closed wrapped in tinfoil and placed poly fill at end of zipper to allow bags to breath,  boiled all for two hours with tinfoil and tops. I poked to holes in each jar top with a nail and I stuffed the holes with poly fill  then I placed tinfoil on the tops did not screw the tops on completely and boiled. After I took all units filled with substrate in oven and allowed to sit and cool over night. I'm morning I cleaned bathroom vigorously and disinfected like three times with whole can of Lysol. Did myself up with rubber gloves and clean clothes and started my inoculation process through the poly fill, I did notice that some redish appearing stuff was on some of my poly fill which I contributed to the wax on the lids Probably from the boiling process. As it was red wax, makes since. I injected half a cc in each hole against the glass into the corn. I did not use a vermiculite topping as I read on here its not nessasary.  I then placed in a dark area in which the temp carries from 75 - 83 and here we are, should I be worried what scares me is I have four strains and see no sign of colonization in any of them yet? Tinfoil is removed. Important note the first day my temp did go to 90 due to heater being to high and that morning go to 68. But I have it perfect

Edited by Newtimer (05/21/13 04:19 PM)

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OfflineLeper
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18222019 - 05/06/13 11:45 AM (11 years, 8 months ago)

Hard to see in popcorn. Took me about 9 days to see anything on mine.


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OfflineGTI337
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18222031 - 05/06/13 11:49 AM (11 years, 8 months ago)

Not to be a dick but this whole thing seems destined for fail.  Also you should delete the source for your spores as they are not a sponsor and you are using their product in an illegal manner.  Spores are for microscopy only..  When using any grain prep you must have a pressure cooker to sterilize your grains. Microwaving does no good for sterilization.  Nothing here seems to have been done even close to proper... Use the search function and read up on teks.  I would advise against corn myself but thats only preference. If you don't have a PC you should stick to PF tek until you get your sterile technique down.


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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18222047 - 05/06/13 11:53 AM (11 years, 8 months ago)

Popcorn should be pressure cooked. I would be surprised if your jars didn't get overrun with bacteria before full colonization.


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OfflineNewtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: Kizzle]
    #18222750 - 05/06/13 02:34 PM (11 years, 8 months ago)

Yeah I wasn't a huge fan about trying with out a PC but my friend had done it before with 12 jars by steaming like I had done above he said the soaking over night causes any spores in grain to germinate which then allows the steaming process to kill them off, I also did some of my own reasearch on that and after youtube and other posts on this site I started to believe in this process knowing others have had great success with the steaming method. Thanks for the input my fingers are crossed. V/R

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OfflineNewtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18226373 - 05/07/13 09:42 AM (11 years, 8 months ago)


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OfflineNewtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18227438 - 05/07/13 02:03 PM (11 years, 8 months ago)

Ok so my corn is starting to look white on the ends and some look like they split, very little are split though. Also my jars look a tiny bit condensated with water will this be ok, kinda looks like it sweat a tiny bit as you can see from pic above. No water build up on bottom though. I'm starting to get a good feeling for success :wink:

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18228016 - 05/07/13 03:57 PM (11 years, 8 months ago)

Hey, nothing but good things, OP!  Time will tell.


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Re: 6 days popcorn substrate no sign mycelium [Re: mpd]
    #18236766 - 05/09/13 07:34 AM (11 years, 8 months ago)

Ok so it's day ten popcorn looks good and tasety and out of my 25 jars no signs of contaminants. Right as I was about to get discouraged I saw a nice patch of mycelium forming on my creepers strain. First one and I'm best excited and hopeful for my additional jars and strains.

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18236778 - 05/09/13 07:40 AM (11 years, 8 months ago)


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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18237396 - 05/09/13 10:38 AM (11 years, 8 months ago)

:thumbup:


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Re: 6 days popcorn substrate no sign mycelium [Re: cronicr]
    #18242871 - 05/10/13 11:19 AM (11 years, 8 months ago)

I need to work on my patients, still only one showing signs/ I re injected all the other jars. Did a fantastic job on sterlization as no jars are showing any contaminats so so much for the posts above. As for now of now I can say that if using clean Popcorn you do not need a pressure cooker. Time will tell if my other jars colonize i will continue this post to provide updates and pics. I'm going to ignore them for the next three days / update on Tuesday :smile:

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18242916 - 05/10/13 11:28 AM (11 years, 8 months ago)

You didn't sterilize correctly and this will almost certainly end in failure. Stick to BRF until you get a PC.

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Re: 6 days popcorn substrate no sign mycelium [Re: NewfoundFreedom]
    #18242930 - 05/10/13 11:30 AM (11 years, 8 months ago)

And stop using corn. Its crap

im dumping my corn jars in an outdoor crop, dont want to waste my indoor space for something that under performs.

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Re: 6 days popcorn substrate no sign mycelium [Re: Fuzz-nutter]
    #18243030 - 05/10/13 11:47 AM (11 years, 8 months ago)

Wow I hope to prove you all wrong, as I have alot of faith in what I have done so far and wouldn't I see contamination by now its been over two weeks?

Edited by Newtimer (05/10/13 11:56 AM)

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18243098 - 05/10/13 12:02 PM (11 years, 8 months ago)

How long did you sterilize it


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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18243111 - 05/10/13 12:05 PM (11 years, 8 months ago)

Quote:

Newtimer said:
Wow I hope to prove you all wrong, as I have alot of faith in what I have done so far and wouldn't I see contamination by now its been over two weeks?




The absence of evidence is not the evidence of absence. In other words, just because you don't see a contaminant doesn't mean there isn't one. Time will tell. If you manage to fully colonize your jars you can at that point smell them before using them as spawn.

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Re: 6 days popcorn substrate no sign mycelium [Re: NewfoundFreedom]
    #18243170 - 05/10/13 12:17 PM (11 years, 8 months ago)

I soaked it for close to 16 hours then after I boiled the popcorn for an hour and let it sit over night. In the morning I placed in in jars and the steamed each batch for two hours then let it sit over night again before inoculation. As I read in other threads and whats posted on you tube allowing popcorn to sit soaked in water for more than 12 hours triggers all grain spores to germinate once this occurs the two hours of steaming with lids loose allows for heat to kill all contaminating grain spores which has seemed to work. I know everyone says its going to fail but I don't think alot of people have ever tried to prepare popcorn like you tube says to so if they have not and only preach pressure cookers than they should not comment because trial and error will determine who's correct. Every step I have taken has been tried and proven true so we will see if I have same results. Slow colonization is normal with popcorn especially with multi sp. inoculation, I have orders a bunch of new syringes from Sponcers  and hope to try another grain soon cakes do not produce enough of a yield for me so waste of time. I did order a PC for next 50 jars but for now this is my fun

Edited by Newtimer (05/10/13 12:28 PM)

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OfflineMr.White
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18243207 - 05/10/13 12:23 PM (11 years, 8 months ago)

I use popcorn in all my grows, jars colonize blazing fast on popcorn, I notice average transfering to bulk, but to me that is a minor loss.

Started off just steaming in a pot too, works perfectly fine without a PC, I just fit more in a PC, also everyone are hot about them.

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18243213 - 05/10/13 12:24 PM (11 years, 8 months ago)

Also I don't mean to sound like an ass, and I thank you all for your input. Growing stuff isn't easy and I'm learning from all of you so thank you all for your input :smile:

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InvisibleNewfoundFreedom
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18243411 - 05/10/13 01:14 PM (11 years, 8 months ago)

Quote:

Newtimer said:
I soaked it for close to 16 hours then after I boiled the popcorn for an hour and let it sit over night. In the morning I placed in in jars and the steamed each batch for two hours then let it sit over night again before inoculation. As I read in other threads and whats posted on you tube allowing popcorn to sit soaked in water for more than 12 hours triggers all grain spores to germinate once this occurs the two hours of steaming with lids loose allows for heat to kill all contaminating grain spores which has seemed to work. I know everyone says its going to fail but I don't think alot of people have ever tried to prepare popcorn like you tube says to so if they have not and only preach pressure cookers than they should not comment because trial and error will determine who's correct. Every step I have taken has been tried and proven true so we will see if I have same results. Slow colonization is normal with popcorn especially with multi sp. inoculation, I have orders a bunch of new syringes from Sponcers  and hope to try another grain soon cakes do not produce enough of a yield for me so waste of time. I did order a PC for next 50 jars but for now this is my fun




Did you come here for advice or did you come to here to argue that your way is correct and that everyone who says otherwise is wrong?

http://www.shroomery.org/forums/showflat.php/Number/10394962#10394962
Quote:

RogerRabbit said:
Quote:

If it can kill whatever is in the jars for the PF tek, so in theory it should be able to kill the same things on whole grains.



Incorrect theory.

Grains such as rye harbor bacterial endospores that can survive many hours at 212F/100C.  Brown rice does not.  That's why we use pressure cookers for whole grains.  Noobs will just have to stick to brf tek or pony up the dough for a pressure cooker if they want success more than a tiny fraction of the time.
RR





http://www.shroomery.org/forums/showflat.php/Number/17867637#17867637
Quote:

RogerRabbit said:
Quote:

Whippy said:
I am just pointing out the fact that the first things I ever tried to sterilize were 4 quarts of grain.  I steamed them 3 days in a row @ 212.  Is it "recommended".  No.  I don't recommend it because what can be done in a Pressure cooker in 1/3 of the time or less. 

Point of the matter, that Fractal sterilization may be not recommended, but if done correctly it accomplishes the means necessary for this hobby.  It does work, and failure is from not adhering to specific schedules.  It must be done every 24 hours....not later in the day.....




No it does not work.  I suggest if you wish to claim it does that you 'fractional sterilize' 100 jars of grains and inoculate them.  If over 50 of those jars produce mushrooms, you can say it has a 50% success/fail rate.  I know for a fact however that you won't be able to get 50 out of 100 jars to fruit that way.

After preparation of the grains, you can steam at 100C for 8 hours and succeed.  In fact, this is the method we use on our commercial mushroom farm to achieve 100% success.  Not only that, but we're at 4000' elevation so water boils at less than 100C.  The key is to keep the grains or supplemented sawdust at or near 100C for 8 hours.

Obviously a pressure cooker or sterilizer is best and quickest, but the above works.
RR




http://www.shroomery.org/forums/showflat.php/Number/16725278#16725278
Quote:

Green molds aren't the problem, they kill easily below boiling. What we sterilize for is bacterial endospores, which can be really tough to kill. Many begin to form new endospores within hours of germination, so if you go too long between boilings, you're screwed. I'd start over and do the three boilings 24 hours apart as suggested in the tek.

Of course, this method has a very high failure rate, so I'd still recommend using the brf tek. There's nothing wrong with it. Many very experienced growers do very well with pf tek, and stick with it even though they're also experts at bulk.
RR



Quote:

If you don't wish to sterilize by traditional proved methods, you can simply boil as in the
brf tek for 8 hours and this will sterilize grains.
  Fractional sterilization is a failed tek that has been disproved for many years.
RR




Edited by NewfoundFreedom (05/10/13 01:28 PM)

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OfflineNewtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: NewfoundFreedom]
    #18243593 - 05/10/13 02:06 PM (11 years, 8 months ago)

Well lets see I'm not doing anything diff.from suggested lets see what horriable predicted success I have, personally I think I'm going to be just fine :smile:

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Re: Wow Day 10 and finally a good sign / pic below [Re: Newtimer]
    #18243732 - 05/10/13 02:33 PM (11 years, 8 months ago)

Quote:

Newtimer said:
Well lets see I'm not doing anything diff.from suggested lets see what horriable predicted success I have, personally I think I'm going to be just fine :smile:





Quote:

Newtimer said:These are the steps I have taken thus far. I soaked Popcorn for 15 hours, rinsed and boiled for hour and half. Then I washed all jars and boiled jars and tops for hour. After that I placed jars in micro wave for 3 min just to be sure zero contamination. Rinsed my popcorn a final time and let dry on towel for two hours until mostly dry "it felt nice and soft but not mushy and not very wet" I then filled jars with popcorn and do not have a pressure cooker so instead I used a huge roaster in which I placed all the jars in covered and boiled i did same with bags just zipped the bags closed wrapped in tinfoil and placed poly fill at end of zipper to allow bags to breath,  boiled all for two hours with tinfoil and tops. I poked to holes in each jar top with a nail and I stuffed the holes with poly fill  then I placed tinfoil on the tops did not screw the tops on completely and boiled. After I took all units filled with substrate in oven and allowed to sit and cool over night. I'm morning I cleaned bathroom vigorously and disinfected like three times with whole can of Lysol. Did myself up with rubber gloves and clean clothes and started my inoculation process through the poly fill, I did notice that some redish appearing stuff was on some of my poly fill which I contributed to the wax on the lids Probably from the boiling process. As it was red wax, makes since. I injected half a cc in each hole against the glass into the corn. I did not use a vermiculite topping as I read on here its not nessasary.  I then placed in a dark area in which the temp carries from 75 - 83 and here we are, should I be worried what scares me is I have four strains and see no sign of colonization in any of them yet? Tinfoil is removed. Important note the first day my temp did go to 90 due to heater being to high and that morning go to 68. But I have it perfect




Quote:

The key is to keep the grains or supplemented sawdust at or near 100C for 8 hours.



Quote:

Fractional sterilization is a failed tek that has been disproved for many years.
RR




:retawed:

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Re: 6 days popcorn substrate no sign mycelium [Re: NewfoundFreedom]
    #18244054 - 05/10/13 03:56 PM (11 years, 8 months ago)

Will see I'm starting 30 more jars with same process

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18244279 - 05/10/13 04:56 PM (11 years, 8 months ago)

Quote:

Will see I'm starting 30 more jars with same process




Reinventing the wheel eh
:goodluckwiththat2:



:cheersyoufuck: Good luck with that

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OfflineKizzle
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18244710 - 05/10/13 06:36 PM (11 years, 8 months ago)

Quote:

Newtimer said:
Wow I hope to prove you all wrong, as I have alot of faith in what I have done so far and wouldn't I see contamination by now its been over two weeks?



You can't see bacteria. Although eventually it can make your grains look wet and then slimy, sometimes you only know it's there because the jars stalls.


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Re: 6 days popcorn substrate no sign mycelium [Re: Kizzle]
    #18244749 - 05/10/13 06:42 PM (11 years, 8 months ago)

How are your one gallon Ziploc bags holding up?  Trying to sterilize and keep medium sterile in Ziploc bags has been real hit or miss - more often a miss for me. 

They contaminate very easily.


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Re: 6 days popcorn substrate no sign mycelium [Re: mycomattie]
    #18244983 - 05/10/13 07:20 PM (11 years, 8 months ago)

You know what dude, you did what you did and even if it doesn't work out, at least you got some of the procedures down, so it's not all in failure if it does contam.

You might want to think about investing in a pressure cooker at some time.
I wish you the best luck....

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Re: 6 days popcorn substrate no sign mycelium [Re: MadSeasonStudent]
    #18247825 - 05/11/13 11:31 AM (11 years, 8 months ago)

Great news I have three out of the four strains colonizing I see mycelium finally in 16 out of 24 jars all but Cambodian. Bag substrate too out of 3 bags two look real good b+ and creeper all looks good thus far / I'm super stoked :wink:

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Two and a half weeks on popcorn [Re: Newtimer]
    #18269841 - 05/15/13 04:47 PM (11 years, 8 months ago)

Almost there

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Re: Two and a half weeks on popcorn [Re: Newtimer]
    #18269859 - 05/15/13 04:51 PM (11 years, 8 months ago)

how cute...shake it


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Re: Two and a half weeks on popcorn [Re: cronicr]
    #18269907 - 05/15/13 04:59 PM (11 years, 8 months ago)

Quote:

cronicr said:
how cute...shake it




:thumbup:

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3 weeks good mycelium / popcorn [Re: heebusjeebus]
    #18279995 - 05/17/13 06:02 PM (11 years, 7 months ago)

Three weeks good things all my creeper is colonizing and looks like pic, my b+ is going nice also. Nothing from Cambodian unfortunately I'm still going to let them try.y equator are just starting and I'm seeing positive growth so I'd have to say all is working Out one bag of b+ still going strong think for fun in going to fruit in bag. Started Mexican, pink buffalo and golden teacher the other day same way because it seems to be working perfect. Because no Cambodian came alive in thinking maybe a spore issue or bacteria but seems weird all others came too but one certain strain. Any way stoked with results loving the process. Going to case with vermiculight and earth worm stuff.

Edited by Newtimer (05/17/13 06:07 PM)

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Re: 3 weeks good mycelium / popcorn [Re: Newtimer]
    #18280845 - 05/17/13 09:11 PM (11 years, 7 months ago)

Glad this worked out for you man

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Re: 6 days popcorn substrate no sign mycelium [Re: Leseid]
    #18282649 - 05/18/13 07:48 AM (11 years, 7 months ago)


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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18282655 - 05/18/13 07:51 AM (11 years, 7 months ago)

How do you shake your jars with it being that full?

anyway looks good, id give popcorn a go if it wasnt so damn expensive for popcorn seed in the UK.

WBS is 85p for 1.5 kg compare to almost £2 for a small bag (probably 500g) of popcorn.


If your fruiting straight grain you could just mix 50/50 with hydrated verm (rez effect) rather than using a casing layer. Will provide all the needed water to support fruiting.


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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18282760 - 05/18/13 08:58 AM (11 years, 7 months ago)

Just this monday i noc'd a jar(2,5 liters by volume), filled with 2dl of popcorn, used a print of Pcola and the first signs of myc growth appeared three days later..

So far so good,  gonna use it for g2g transfers. My 2nd attempt to grow(ever) btw. frst one failed miserably, scored a contam hat trick with green&black mold and what i suspect was a bacterial growth(smelled like strawberry yoghurt). Came to a conclusion that its time to do some serious spring cleaning :grin: 2nd conclusion was that my grain-to-spore ratio was a  little optimistic grain-wise

good luck with your grow dude!


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Re: 6 days popcorn substrate no sign mycelium [Re: rulesq]
    #18282771 - 05/18/13 09:06 AM (11 years, 7 months ago)

Quote:

rulesq said:
Just this monday i noc'd a jar(2,5 liters by volume), filled with 2dl of popcorn, used a print of Pcola and the first signs of myc growth appeared three days later..

So far so good,  gonna use it for g2g transfers. My 2nd attempt to grow(ever) btw. frst one failed miserably, scored a contam hat trick with green&black mold and what i suspect was a bacterial growth(smelled like strawberry yoghurt). Came to a conclusion that its time to do some serious spring cleaning :grin: 2nd conclusion was that my grain-to-spore ratio was a  little optimistic grain-wise

good luck with your grow dude!





Are you using a still air box, any attempts i made at inoculating without a still air box usually ended in 90% failure to molds. If your not using a SAB you could well be contaminating syringes you make from a print as well as the print itself.


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Re: 6 days popcorn substrate no sign mycelium [Re: veda_sticks]
    #18282800 - 05/18/13 09:16 AM (11 years, 7 months ago)

I'm not condoning the lack of a pressure cooker, but what people often do not mention, is how rye in particular has more bacterial issues than other grains. 

  Odd as it may be, I've seen folks in Europe pull off these popcorn grows with no PC.


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Re: 6 days popcorn substrate no sign mycelium [Re: Oeric McKenna]
    #18282832 - 05/18/13 09:30 AM (11 years, 7 months ago)

It shakes up just fine, i inject in three spots middle and both sides. Colonized thick and steady in middle first. Believe it or not I'm not using still air box just small bathroom which is sprayed to hell with Lysol. I even took a real healthy jar of creeper and opened it five days ago and opened three jars that were not showing mycelium. Scraped out some healthy whites and tossed em in the other jars. They took like wild fire. Showing fast colonization. So far I've been pretty lucky with no molds and great results out if all my jars now only five have nothing all the one strain of Cambodian. All others are beautiful white fuzzys eating up all the popcorn in their warm beds. Can't wait to see how my new three strains take to the corn, split half white/ yellow corn. I also heard to fruit with something nutritional is that the case or popcorn would be good alone with vermiculight?

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Re: 6 days popcorn substrate no sign mycelium [Re: veda_sticks]
    #18282833 - 05/18/13 09:30 AM (11 years, 7 months ago)

SAB for sure, jst the first time i tried this, my apartment was seriously a mess. Hadn't cleaned it properly for the whole winter or so.. could have been bad prints as well, who knows but i still would say it was due to bad preparations

this time i cleaned my apt thoroughly, used more sterile methods(like spraying Iso so much that i nearly fainted lol) and used far less popcorn compared to last time. :smile:

Quote:

Odd as it may be, I've seen folks in Europe pull off these popcorn grows with no PC




this is exactly what im doing, following a tek  i found from a Finnish forum (no pc, no SAB although i use one)


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Re: 6 days popcorn substrate no sign mycelium [Re: rulesq]
    #18283565 - 05/18/13 12:59 PM (11 years, 7 months ago)

If you're going to spray things in your air for mold spores, use a mix of bleach/water.


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Re: 6 days popcorn substrate no sign mycelium [Re: Oeric McKenna]
    #18283593 - 05/18/13 01:07 PM (11 years, 7 months ago)

Heheh thats what i did,  forgot to mention abt it. i sprayed iso as well, just to calm my nerves i guess :shrug:


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Re: 6 days popcorn substrate no sign mycelium [Re: rulesq]
    #18283735 - 05/18/13 01:38 PM (11 years, 7 months ago)

All good man. Wasn't trying to correct you either, just a suggestion. It works really good.
Good ol' bleach!


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Re: 6 days popcorn substrate no sign mycelium [Re: Oeric McKenna]
    #18283856 - 05/18/13 01:47 PM (11 years, 7 months ago)



Get that bleach! :rockon:


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Re: 6 days popcorn substrate no sign mycelium [Re: rulesq]
    #18284248 - 05/18/13 03:43 PM (11 years, 7 months ago)

Quote:

rulesq said:
SAB for sure, jst the first time i tried this, my apartment was seriously a mess. Hadn't cleaned it properly for the whole winter or so.. could have been bad prints as well, who knows but i still would say it was due to bad preparations

this time i cleaned my apt thoroughly, used more sterile methods(like spraying Iso so much that i nearly fainted lol) and used far less popcorn compared to last time. :smile:

Quote:

Odd as it may be, I've seen folks in Europe pull off these popcorn grows with no PC




this is exactly what im doing, following a tek  i found from a Finnish forum (no pc, no SAB although i use one)




Having a clean work area is always a good thing, though some people tend to go over the top when its not realy nessesary.

With a SAB you dont have to bleach/lysol/oust bomb everything , dress up like your in a toxic waste contaminated place with radiation suit and gas mask goggles etc.

to which you might get something in the air land on your injection site to be pushed into your jar.

some people have no problems with inoculating in open air in a small room but some have terrible issues. which is why its not a recomended method. We like repeatable proven methods based on solid science.

In a SAB, confined space the air settles and so do contams, leave the walls wet so that just incase any air does get moved contams are less likely to go back into the air.

Gloves stop you shedding contaminated skin cells over everything aswell as wearing long sleaves so your arm isnt doing the same.

Its much easier to ensure nothingis floating around landing on stuff within a SAB than it is an entire room. Plus your not breathing inside the box.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Re: 6 days popcorn substrate no sign mycelium [Re: veda_sticks]
    #18286884 - 05/19/13 07:57 AM (11 years, 7 months ago)

Just for the hell of it I have taken two jars of Cambodian that never produced mycelium and dumbed the corn into a tupper ware bin, where i mixed in some earth worm casings and then I took a fully colonized jar of creeper and broke it all up and mixed it with the other substrate. It smelled so good like strong fresh mushrooms. It was perfect even felt like mushroomy when I was breaking it up. I'm super stoked for this exsperiment and have been having the best of luck with my first grow ever. Even my second batch of jars is already starting to colonize with white corn. Same three strains creeper/ B+ / equator, having same issue with Cambodian. I'm ordering new spores of cam so I deff think it might have been spores. Should start seeing groth with in next week for my Golden Teacher/ Pink Buffalo/ Mexican. This has been so much fun so far can't wait to fruit. Grain to Grain transfer seems to be the best way for me. Once I see strong mycelium in one jar I spoon some in my sterilized substrate and it spreads like wild fire my jars move 5 times faster.  With no pressure cooker 19 of my jars out of 24 are about 3 days away from full colonization. Five Cambodian never developed anything but stayed fresh. Which I've left two to see if anything but the other three just reused the substrate for more food for creeper.

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18291970 - 05/20/13 10:10 AM (11 years, 7 months ago)

Bulk sub colonizing pretty on corn mixed with earth worm stuff.

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18292028 - 05/20/13 10:23 AM (11 years, 7 months ago)

Quote:

Newtimer said:
Just for the hell of it I have taken two jars of Cambodian that never produced mycelium and dumbed the corn into a tupper ware bin, where i mixed in some earth worm casings and then I took a fully colonized jar of creeper and broke it all up and mixed it with the other substrate.




Quote:

Newtimer said:
Once I see strong mycelium in one jar I spoon some in my sterilized substrate and it spreads like wild fire my jars move 5 times faster.  With no pressure cooker 19 of my jars out of 24 are about 3 days away from full colonization. Five Cambodian never developed anything but stayed fresh. Which I've left two to see if anything but the other three just reused the substrate for more food for creeper.




Quote:

Newtimer said:
Bulk sub colonizing pretty on corn mixed with earth worm stuff.





I wanna see how this turns out. I have an idea already, but watching a train wreck can be fun. There might be a survivor or two :wink:

:popcorn:

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Re: 6 days popcorn substrate no sign mycelium [Re: Pastywhyte]
    #18292058 - 05/20/13 10:30 AM (11 years, 7 months ago)

Quote:

Pastywhyte said:
Quote:

Newtimer said:
Just for the hell of it I have taken two jars of Cambodian that never produced mycelium and dumbed the corn into a tupper ware bin, where i mixed in some earth worm casings and then I took a fully colonized jar of creeper and broke it all up and mixed it with the other substrate.




Quote:

Newtimer said:
Once I see strong mycelium in one jar I spoon some in my sterilized substrate and it spreads like wild fire my jars move 5 times faster.  With no pressure cooker 19 of my jars out of 24 are about 3 days away from full colonization. Five Cambodian never developed anything but stayed fresh. Which I've left two to see if anything but the other three just reused the substrate for more food for creeper.




Quote:

Newtimer said:
Bulk sub colonizing pretty on corn mixed with earth worm stuff.





I wanna see how this turns out. I have an idea already, but watching a train wreck can be fun. There might be a survivor or two :wink:

:popcorn:




oh the humanity :grin:


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Re: 6 days popcorn substrate no sign mycelium [Re: rulesq]
    #18292317 - 05/20/13 11:29 AM (11 years, 7 months ago)

I know I'm having fun, hey from day one I was told my jars will contaminate and will not 100 percent colonize. That I'm destined to fail and nothing was done right. Ha is all I have to say cause here I am with almost all my original jars at full colonization zero contams, and smelling like fresh delicious mushrooms. I was told popcorn is bad but yet I'm having a blast using it and the mycelium love it. Shit I even have enough jars  colonized that I have experimented already. So here I am doing everything wrong I guess but having it all work out so right. So as for my experiment I'm guessing it will turn out wonderful just as the rest of my grow. I'm not promising seeing I truly do really no nothing about mushrooms except how much fun it is to learn and grow. So far though its really been wonderful and I think I'm having more fun succeeding thus far because everyone said it was doomed from the get go. Don't worry I'll take pics of the train wreck when I get beautiful potent creepers :wink: it's already tripled in colonization so it must be doing something right.

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18292449 - 05/20/13 11:56 AM (11 years, 7 months ago)

Yeah man i'm not being skeptical here, i'm kinda doing the same, using popcorn, getting my feet wet first with it until im getting a PC, thats the only thing missing from my sterilization/sanitizing tools :shrug:

PC coming by the end of the month, already had one but it turned out to be way too small for my purposes, when i find a suitable one, it's rye all the way from there.

Nothing but high hopes for your project man :heart:


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Re: 6 days popcorn substrate no sign mycelium [Re: rulesq]
    #18293347 - 05/20/13 03:30 PM (11 years, 7 months ago)

So today one of my two jars of Cambodian finally has a tiny spec of fuzzy mycelium, whipping up some more popcorn substrate to get some g2g transfers started once and if it gets healthy and strong enough. I hope so cause that's suppose to be a good one. This will make my first grow complete all four strains. Lovin life :wink: yeah buddy

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18293451 - 05/20/13 03:50 PM (11 years, 7 months ago)

So happy / Cambodian mycelium finally almost four weeks before this tiny friend appeared I just about lost hope. Listen if your gonna post nonsence like its not going to colonize or other bull don't bother cause your wrong and I'm sure it will, the best thing is you will see it go from zero to hero :wink:


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This is my white popcorn and B+ 2 weeks [Re: Newtimer]
    #18293676 - 05/20/13 04:42 PM (11 years, 7 months ago)

Even with split kerrnals it took two weeks for this, much faster than the yellow but then the kerrnals size is smaller in the white so I'm thinking that had something to do with it. Also used 2cc's at point of inoculation.

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Re: 6 days popcorn substrate no sign mycelium [Re: Kizzle]
    #18297920 - 05/21/13 12:19 PM (11 years, 7 months ago)

No jars contaminated and all colonized

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18308593 - 05/23/13 10:38 AM (11 years, 7 months ago)

Good Stuff - pink buff. Golden T and Mex are showing mycelium. Also did some huge tote transfers g2g today with creep and B+ doing monotub meathod using corn is great because all the moisture it holds I see lots of dew on sides :smile: - going to start fruiting a tub tomorrow so excited all my hard work is almost paid off. Thanks to this amazing web site and everyone's help in this process. So far everything has gone so much better than I ever thought and its all cause of this site. Can't wait to show pics of mushies for ya all of this first attempt. Have a great weekend all.

Edited by Newtimer (05/23/13 11:10 AM)

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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
    #18362836 - 06/03/13 02:36 PM (11 years, 7 months ago)

Progress?


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Re: 6 days popcorn substrate no sign mycelium [Re: Kizzle]
    #18362872 - 06/03/13 02:42 PM (11 years, 7 months ago)

Quote:

Kizzle said:
Progress?



:justdontknow:


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Re: 6 days popcorn substrate no sign mycelium [Re: B_BOY]
    #18362965 - 06/03/13 03:04 PM (11 years, 7 months ago)

I wanna see the beautiful potent creepers that were promised a few posts up :lol:

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Re: 6 days popcorn substrate no sign mycelium [Re: Pastywhyte]
    #18425090 - 06/15/13 09:17 PM (11 years, 7 months ago)

OP didn't deliver. a lovely crop of cobweb and trich probably did though

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Re: 6 days popcorn substrate no sign mycelium [Re: Fuzz-nutter]
    #18425201 - 06/15/13 09:45 PM (11 years, 7 months ago)


Bacteria probably. If I'm not mistaken there were already signs of it in this jar.


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Re: 6 days popcorn substrate no sign mycelium [Re: Kizzle]
    #18425241 - 06/15/13 09:56 PM (11 years, 7 months ago)

I read it all but there was no end.
Blue balls. :sad::sad:


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Re: 6 days popcorn substrate no sign mycelium [Re: Eggtimer] * 1
    #18425279 - 06/15/13 10:05 PM (11 years, 7 months ago)

Quote:

Eggtimer said:
I read it all but there was no end.
Blue balls. :sad::sad:




I knew how it would end from the start. I think most people did. By the time he got to mixing uncolonized grain into his sub the writing was on the wall. Hopefully OP does some good research and gets it right next time.

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Re: 6 days popcorn substrate no sign mycelium [Re: Pastywhyte]
    #18427122 - 06/16/13 11:00 AM (11 years, 6 months ago)

Odd, they've been on as recently as 24hrs ago.

No updates for the last 22 days?


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