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Newtimer
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Registered: 05/06/13
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Last seen: 11 years, 6 months
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4 Weeks popcorn no PC and it went great 100% colonization
#18221992 - 05/06/13 11:40 AM (11 years, 8 months ago) |
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Hello, as you Can already tell from my name on here im new at this, my first grow experience. I inoculated 24 jars pint-size jars six days ago along with three 1 gallon Ziploc bags half full of substrate and one two pint jar. I inoculated them with four different strains with spore syringes include creeper, B , Ecuador and Cambodian now it's been six days and I see no mycelium growth which makes me nervous, i also see no contamination factors either. I'm using yellow popcorn which I purchased from grocery store. These are the steps I have taken thus far. I soaked Popcorn for 15 hours, rinsed and boiled for hour and half. Then I washed all jars and boiled jars and tops for hour. After that I placed jars in micro wave for 3 min just to be sure zero contamination. Rinsed my popcorn a final time and let dry on towel for two hours until mostly dry "it felt nice and soft but not mushy and not very wet" I then filled jars with popcorn and do not have a pressure cooker so instead I used a huge roaster in which I placed all the jars in covered and boiled i did same with bags just zipped the bags closed wrapped in tinfoil and placed poly fill at end of zipper to allow bags to breath, boiled all for two hours with tinfoil and tops. I poked to holes in each jar top with a nail and I stuffed the holes with poly fill then I placed tinfoil on the tops did not screw the tops on completely and boiled. After I took all units filled with substrate in oven and allowed to sit and cool over night. I'm morning I cleaned bathroom vigorously and disinfected like three times with whole can of Lysol. Did myself up with rubber gloves and clean clothes and started my inoculation process through the poly fill, I did notice that some redish appearing stuff was on some of my poly fill which I contributed to the wax on the lids Probably from the boiling process. As it was red wax, makes since. I injected half a cc in each hole against the glass into the corn. I did not use a vermiculite topping as I read on here its not nessasary. I then placed in a dark area in which the temp carries from 75 - 83 and here we are, should I be worried what scares me is I have four strains and see no sign of colonization in any of them yet? Tinfoil is removed. Important note the first day my temp did go to 90 due to heater being to high and that morning go to 68. But I have it perfect
Edited by Newtimer (05/21/13 04:19 PM)
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Leper
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18222019 - 05/06/13 11:45 AM (11 years, 8 months ago) |
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Hard to see in popcorn. Took me about 9 days to see anything on mine.
-------------------- Leper is a fictitious entity using images supplied by google to mask his/her inability to form normal social habits within society.
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GTI337
omnomnomnom



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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18222031 - 05/06/13 11:49 AM (11 years, 8 months ago) |
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Not to be a dick but this whole thing seems destined for fail. Also you should delete the source for your spores as they are not a sponsor and you are using their product in an illegal manner. Spores are for microscopy only.. When using any grain prep you must have a pressure cooker to sterilize your grains. Microwaving does no good for sterilization. Nothing here seems to have been done even close to proper... Use the search function and read up on teks. I would advise against corn myself but thats only preference. If you don't have a PC you should stick to PF tek until you get your sterile technique down.
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Kizzle
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18222047 - 05/06/13 11:53 AM (11 years, 8 months ago) |
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Popcorn should be pressure cooked. I would be surprised if your jars didn't get overrun with bacteria before full colonization.
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Newtimer
Stranger


Registered: 05/06/13
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Re: 6 days popcorn substrate no sign mycelium [Re: Kizzle]
#18222750 - 05/06/13 02:34 PM (11 years, 8 months ago) |
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Yeah I wasn't a huge fan about trying with out a PC but my friend had done it before with 12 jars by steaming like I had done above he said the soaking over night causes any spores in grain to germinate which then allows the steaming process to kill them off, I also did some of my own reasearch on that and after youtube and other posts on this site I started to believe in this process knowing others have had great success with the steaming method. Thanks for the input my fingers are crossed. V/R
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Newtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18226373 - 05/07/13 09:42 AM (11 years, 8 months ago) |
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Newtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18227438 - 05/07/13 02:03 PM (11 years, 8 months ago) |
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Ok so my corn is starting to look white on the ends and some look like they split, very little are split though. Also my jars look a tiny bit condensated with water will this be ok, kinda looks like it sweat a tiny bit as you can see from pic above. No water build up on bottom though. I'm starting to get a good feeling for success
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mpd
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18228016 - 05/07/13 03:57 PM (11 years, 8 months ago) |
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Hey, nothing but good things, OP! Time will tell.
-------------------- There is no truer calling for mankind than that of true conservatism.
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Newtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: mpd]
#18236766 - 05/09/13 07:34 AM (11 years, 8 months ago) |
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Ok so it's day ten popcorn looks good and tasety and out of my 25 jars no signs of contaminants. Right as I was about to get discouraged I saw a nice patch of mycelium forming on my creepers strain. First one and I'm best excited and hopeful for my additional jars and strains.
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Newtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18236778 - 05/09/13 07:40 AM (11 years, 8 months ago) |
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cronicr



Registered: 08/07/11
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18237396 - 05/09/13 10:38 AM (11 years, 8 months ago) |
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-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Newtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: cronicr]
#18242871 - 05/10/13 11:19 AM (11 years, 8 months ago) |
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I need to work on my patients, still only one showing signs/ I re injected all the other jars. Did a fantastic job on sterlization as no jars are showing any contaminats so so much for the posts above. As for now of now I can say that if using clean Popcorn you do not need a pressure cooker. Time will tell if my other jars colonize i will continue this post to provide updates and pics. I'm going to ignore them for the next three days / update on Tuesday
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NewfoundFreedom
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Registered: 05/06/04
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18242916 - 05/10/13 11:28 AM (11 years, 8 months ago) |
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You didn't sterilize correctly and this will almost certainly end in failure. Stick to BRF until you get a PC.
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Fuzz-nutter


Registered: 04/06/13
Posts: 1,740
Loc: Canada
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Re: 6 days popcorn substrate no sign mycelium [Re: NewfoundFreedom]
#18242930 - 05/10/13 11:30 AM (11 years, 8 months ago) |
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And stop using corn. Its crap
im dumping my corn jars in an outdoor crop, dont want to waste my indoor space for something that under performs.
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Newtimer
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Registered: 05/06/13
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Re: 6 days popcorn substrate no sign mycelium [Re: Fuzz-nutter]
#18243030 - 05/10/13 11:47 AM (11 years, 8 months ago) |
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Wow I hope to prove you all wrong, as I have alot of faith in what I have done so far and wouldn't I see contamination by now its been over two weeks?
Edited by Newtimer (05/10/13 11:56 AM)
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cronicr



Registered: 08/07/11
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18243098 - 05/10/13 12:02 PM (11 years, 8 months ago) |
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How long did you sterilize it
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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NewfoundFreedom
Manure whore


Registered: 05/06/04
Posts: 1,915
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18243111 - 05/10/13 12:05 PM (11 years, 8 months ago) |
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Quote:
Newtimer said: Wow I hope to prove you all wrong, as I have alot of faith in what I have done so far and wouldn't I see contamination by now its been over two weeks?
The absence of evidence is not the evidence of absence. In other words, just because you don't see a contaminant doesn't mean there isn't one. Time will tell. If you manage to fully colonize your jars you can at that point smell them before using them as spawn.
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Newtimer
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Re: 6 days popcorn substrate no sign mycelium [Re: NewfoundFreedom]
#18243170 - 05/10/13 12:17 PM (11 years, 8 months ago) |
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I soaked it for close to 16 hours then after I boiled the popcorn for an hour and let it sit over night. In the morning I placed in in jars and the steamed each batch for two hours then let it sit over night again before inoculation. As I read in other threads and whats posted on you tube allowing popcorn to sit soaked in water for more than 12 hours triggers all grain spores to germinate once this occurs the two hours of steaming with lids loose allows for heat to kill all contaminating grain spores which has seemed to work. I know everyone says its going to fail but I don't think alot of people have ever tried to prepare popcorn like you tube says to so if they have not and only preach pressure cookers than they should not comment because trial and error will determine who's correct. Every step I have taken has been tried and proven true so we will see if I have same results. Slow colonization is normal with popcorn especially with multi sp. inoculation, I have orders a bunch of new syringes from Sponcers and hope to try another grain soon cakes do not produce enough of a yield for me so waste of time. I did order a PC for next 50 jars but for now this is my fun
Edited by Newtimer (05/10/13 12:28 PM)
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Mr.White
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18243207 - 05/10/13 12:23 PM (11 years, 8 months ago) |
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I use popcorn in all my grows, jars colonize blazing fast on popcorn, I notice average transfering to bulk, but to me that is a minor loss.
Started off just steaming in a pot too, works perfectly fine without a PC, I just fit more in a PC, also everyone are hot about them.
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Newtimer
Stranger


Registered: 05/06/13
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Re: 6 days popcorn substrate no sign mycelium [Re: Newtimer]
#18243213 - 05/10/13 12:24 PM (11 years, 8 months ago) |
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Also I don't mean to sound like an ass, and I thank you all for your input. Growing stuff isn't easy and I'm learning from all of you so thank you all for your input
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