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OfflineCJCollin37
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My Trich-Eating Isolate!
    #18194397 - 04/30/13 09:28 PM (10 years, 9 months ago)

I just isolated a bunch of strains from multispore on agar and knocked up about 20 jars of WBS with the various strains plus a clone to see which is the best. My best looking jar was of course the only one to contaminate with some hearty trich a week or two after inoculation.

The mycelium is BY FAR the most beautiful I've ever seen. Long, super ropey rhizomorphs emanated from one single point with no sectoring. At the surface, long ropes of rhizos stretch upward off the WBS. It's crazy. Anyway, I left the jar alone and the mycelium seems to have taken over the trich. It fully colonized the jar and I can't even see the trich anymore!!

I think I'm going to take it outside and put it in its own terrarium in some coir and see if it fruits. If so I think I might have a super strain.

Thoughts? Opinions? Advice? Has anyone done this?

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Offlinesmegzilla69
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Re: My Trich-Eating Isolate! [Re: CJCollin37]
    #18194404 - 04/30/13 09:30 PM (10 years, 9 months ago)

:popcorn:


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WORTH EVERY PENNY!!!!watch it pay for it self in # of high yield flushes!
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Offlinesmurf_master
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Re: My Trich-Eating Isolate! [Re: smegzilla69]
    #18194468 - 04/30/13 09:43 PM (10 years, 9 months ago)

I had a pf cake eat some trich once, I was impressed and it still gave me 3 flushes!


I think it was the Beethoven

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Offlinedodgem
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Re: My Trich-Eating Isolate! [Re: smurf_master]
    #18194569 - 04/30/13 10:13 PM (10 years, 9 months ago)

From what I have gathered from a couple different threads recently is that mycelium will begin to grow very long and ropy, like yours, when it is in the presence of some type of contamination.  I doubt that you would have seen this long and ropy mycelium if your jar had not contaminated.

I will try and find the thread I am thinking of that relates to this.

edit - found it. 

Quote:

Tmethyl said:
The way I describe it is; The healthy cubensis mycelium has met with a contaminant mycelium, and is showing a defensive or flight interaction.
On the left hand side of the cubensis mycelium, you see what it should look like, and where the 2 fungi meet the cubensis rhizomorphs suddenly become stringy, and faster growing.

You also get stringy rhizomorphs in the presence of bacteria.




From here http://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/39/vc/1



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Walk where you like your steps

Edited by dodgem (04/30/13 10:16 PM)

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Offlinefirst time expert
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Re: My Trich-Eating Isolate! [Re: dodgem]
    #18194951 - 04/30/13 11:52 PM (10 years, 9 months ago)

I was also thinking that!!lol But I have a dish that clearly had dusty trich, I just kicked it aside about 3 weeks ago. I looked at it today and the myc has grown over the trich and you can barely see the trich any more. It also was an iso?? Maybe the more spores and shrooms that are produced inside, they might become "resistant" to disease?? Just a guess.


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OfflineTFpek
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Re: My Trich-Eating Isolate! [Re: first time expert]
    #18195013 - 05/01/13 12:13 AM (10 years, 9 months ago)

Keep in mind that trich is white, and it turns green when it sporulates. Those spores germinate and turn white again, so a trich jar left alone will eventually turn white again, and then green, and then white, and so on.

That said, if someone can breed a super strain sign me up for a spore print! Just tossed ten g2g child quart jars today, all spawned from the same dirty rotten spawn jar! the other 36 from my 4 other mother jars are doing alright :smile:

Edited by TFpek (05/01/13 12:15 AM)

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Offlinefirst time expert
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Re: My Trich-Eating Isolate! [Re: TFpek]
    #18195044 - 05/01/13 12:21 AM (10 years, 9 months ago)

Ill have to put up a pic of my dish. Ive never had a jar recover from trich. It maybe a possibility it was penicillin but not sure if its also green and dusty?


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OfflineLeper
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Re: My Trich-Eating Isolate! [Re: first time expert] * 1
    #18195303 - 05/01/13 01:52 AM (10 years, 9 months ago)

I've read in a few places that you can never really get rid of trich. :shrug:
It's a fungicidal mold, not a competitor, which means it's actually eating your mycelium.
I don't mean to rain on your parade, but it may have been either a different contam, or the trich is still hanging out.


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Leper is a fictitious entity using images supplied by google to mask his/her inability to form normal social habits within society.

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Offlineveda_sticks
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Re: My Trich-Eating Isolate! [Re: Leper] * 1
    #18195429 - 05/01/13 02:59 AM (10 years, 9 months ago)

yeah you never win against molds, either you cube myc grows over it and just hides it, or the spores you seen germinated and grew more mold mycelium.

most molds mycelium is white.


--------------------

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Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
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OfflineMaJiK_420
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Re: My Trich-Eating Isolate! [Re: veda_sticks]
    #18195485 - 05/01/13 03:45 AM (10 years, 9 months ago)

My 3rd attempt at a semi sterile agar dish had two green spots that didn't grow after they inittially showed up. Since then the clone tissue has grown and expanded and like you guys say, it has long ropes over the green spots, and then normalizes.

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OfflineWavelength420
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Re: My Trich-Eating Isolate! [Re: MaJiK_420]
    #18195712 - 05/01/13 06:26 AM (10 years, 9 months ago)

Cube myc can't eat trich spores or trich myc

/thread

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OfflineCJCollin37
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Re: My Trich-Eating Isolate! [Re: Wavelength420]
    #18195838 - 05/01/13 07:20 AM (10 years, 9 months ago)

Oops. False alarm I suppose. Bummer.

It's interesting though because long before any mold was visible, from the very first spot of myc, it was very aggressive and ropey with nice rhizos. Then a week or two later, mold showed up on the other side of the jar. The myc kept growing and eventually came into contact with the mold and at that point, the rhizos sort of stopped and it was just regular mycelium that grew over the mold. So that's sort of the opposite of what dodgem is implying.

Is it really not possible for mycelium to sort of suffocate the mold and kill it? Doesn't mold need nutrients or air or something? If the mycelium is more agressive and has effectively covered it and eats all nutrients, couldn't the mold die?

Also, I guess I'm bot sure it's trich. It was green and sort of powdery.

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OfflineLeper
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Re: My Trich-Eating Isolate! [Re: CJCollin37]
    #18196634 - 05/01/13 11:41 AM (10 years, 9 months ago)

If it's trich, it's eating your mycelium.
Trichoderma gets some nutrients from your substrate, but MOST of its nutrients come from eating mushroom mycelium.

The mycelium simply can't compete with something that's eating it.
It's like racing a lion to a steak: The lion's going to think somewhere along the line that a whole human is worth more than a little steak, and gnaw your legs off.

Trich can't get enough of cubie mycelium, just like we can't get enough of cubies. :P


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InvisibleFrankHorrigan
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Re: My Trich-Eating Isolate! [Re: Leper]
    #18196656 - 05/01/13 11:47 AM (10 years, 9 months ago)

Trich is fungicidal. Nothing wins against trich.

Trich will eat your whole plate in under a week.

What I've seen many times on agar is mycelium overtaking sporulated penicillium, a very different mold. It's not uncommon IME. Just don't think to use that to inoculate anything :thumbup:


--------------------

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You should take a look. :hehehe:


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OfflineCJCollin37
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Re: My Trich-Eating Isolate! [Re: FrankHorrigan]
    #18213566 - 05/04/13 02:25 PM (10 years, 9 months ago)

A mushroom is now growing in the jar. I kind of want to just open the jar outside and see if it fruits....

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OfflineLeper
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Re: My Trich-Eating Isolate! [Re: CJCollin37]
    #18213683 - 05/04/13 02:51 PM (10 years, 9 months ago)

It'll fruit, probably.
Early fruiting is a sign of contamination.
But you'll also jeopardize your future grows.


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Leper is a fictitious entity using images supplied by google to mask his/her inability to form normal social habits within society.

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