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Offlineotis5
free thinker

Registered: 08/16/03
Posts: 162
Loc: Why do you want to know w...
Last seen: 8 years, 6 months
dry spores
    #1821288 - 08/16/03 11:45 PM (14 years, 5 days ago)

is there any way to extract a spore from an already dry cap?


--------------------
"he who makes a beast of himself gets rid of the pain of being a man":rasta:


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InvisibleDatDaNK420
SeriousShroomage
Registered: 06/23/03
Posts: 612
Re: dry spores [Re: otis5]
    #1821308 - 08/16/03 11:54 PM (14 years, 5 days ago)

Rehydrate the cap but the chances of contamination are about 99.9% i'd say, so good luck let me know how it works out for you.


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: dry spores [Re: otis5]
    #1821539 - 08/17/03 01:33 AM (14 years, 5 days ago)

sterilize a small piece of wet egg carton in a 1/2 pint jar .
use tweezers to place a gill from said mushroom on the cardboard, and incubate at 80-84F .
if and when the gill turns white,(give it a couple of days)inject some liquid culture medium  with with h2o2 into the jar .

if this doesn't make sense to you read the FAQs on this site, and at nansnook until it does .

that's what i did, and now i am a happy man .  :laugh: 


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OfflineAnAshA
Lil' HippieShroomite
Registered: 06/19/03
Posts: 42
Last seen: 12 years, 4 months
Re: dry spores [Re: deanofmean]
    #1821557 - 08/17/03 01:40 AM (14 years, 5 days ago)

If there are some viable spores left in it at all couldnt you just put it in a jar with some corn syrup? contam would prolly take over but thats just from whats on the cap... thats how I would try it at least... but im just a newb


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: dry spores [Re: AnAshA]
    #1821604 - 08/17/03 02:08 AM (14 years, 5 days ago)

Quote:

AnAshA said:
contam would prolly take over but thats just from whats on the cap



true . that's why most of the pros use agar .
i use the cardboard because  most  contams don't like it .(another thing i learned here on the shroomery message boards)and h2o2 keeps them in check  :smile:
win some lose some . but, when you win with somthing so simple it is sweet . 


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Offlineotis5
free thinker

Registered: 08/16/03
Posts: 162
Loc: Why do you want to know w...
Last seen: 8 years, 6 months
Re: dry spores [Re: deanofmean]
    #1823097 - 08/17/03 04:43 PM (14 years, 4 days ago)

thanks man, that really helps alot :smile:. how would i sterilize the egg carton and what should i use as a culture medium? can you tell im new at this? hehe. :oogle:


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