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OfflineQuailOakinit
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nutrient agar + dry malt extract light ??
    #18204225 - 05/02/13 06:53 PM (11 years, 19 days ago)

I have light dry malt extract from mycopath, and this store bought nutrient agar. I just read that "nutrient agar" is a bad choice for mycelium growth, but I went ahead and mixed 9g nutrient agar to 10g light malt extract and 500ml water. It is currently pressure cooking and I will be pouring my dishes soon.

Although I am going to go ahead and attempt to clone some mushies with this mixture, I wanted to see if  you guys had any experience with this mixture?? am I doomed for sure???

I do not yet have a flowhood, but I do have a pretty big and comfortable glove box to work in, I can fit 35 jars in there with room to work!

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OfflineQuailOakinit
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Re: nutrient agar + dry malt extract light ?? [Re: QuailOakinit]
    #18207269 - 05/03/13 09:26 AM (11 years, 18 days ago)



Well here is the glovebox and my 20 dishes, wish me luck?

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Offlineforrest
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Re: nutrient agar + dry malt extract light ?? [Re: QuailOakinit]
    #18207445 - 05/03/13 10:20 AM (11 years, 18 days ago)

nice still-air-box, good luck!


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OfflineQuailOakinit
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Re: nutrient agar + dry malt extract light ?? [Re: forrest]
    #18207660 - 05/03/13 11:11 AM (11 years, 18 days ago)

Well the deed is done and now it's waiting time.

Edited by QuailOakinit (05/03/13 01:19 PM)

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Invisiblefastfred
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Re: nutrient agar + dry malt extract light ?? [Re: forrest]
    #18209219 - 05/03/13 04:37 PM (11 years, 18 days ago)

Quote:

forrest said:
nice still-air-box, good luck!




It's not a still-air-box, it's a GB.  Don't go confusing him now.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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OfflineNobitte
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Re: nutrient agar + dry malt extract light ?? [Re: fastfred]
    #18212135 - 05/04/13 07:36 AM (11 years, 17 days ago)

I suppose it depends whats in the 'nutrient agar mix', the wikipedia on generic nutrient agar mix (for bacterial culture) states that it contains;

0.5% Peptone
0.3% Beef/yeast extract
1.5% Agar
0.5% NaCl
at pH 6.8

Mixing LME with it should make it a viable medium for fungi, i wonder though, is it possible that the mixed medium will be more condusive to bacteria growth than a straight MEA?

I would predict that the fungi would be fine on the medium, perhaps even better than straight MEA, although you may encounter more obvious bacterial contamination(although that shouldnt be a problem given that youre cloning, which usually involves fairly vigorous growth not too long after transferring(relative to MS))

Also nice GB, much better than what i used to use when i started (Box with cling wrap viewing window and a series of curtains to put my hands under =3). Should work great, but you will definately enjoy having a flowhood if you get one someday <3, oh the ease!.

FF, a question if i may, whats the difference between a still air box and a glove box? The gloves?

Good luck with your clones/agar work, and let me/us know how it all works out ^^


--------------------
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OfflineQuailOakinit
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Re: nutrient agar + dry malt extract light ?? [Re: Nobitte]
    #18213140 - 05/04/13 12:56 PM (11 years, 17 days ago)

Cool, well I went out yesterday an found some "telephone" agar and pressure cooked it up last night. Same ratios as before. I reheated it this morning as it had cooled too much, and will be pouring another round of dishes.

Thanks Nobitte, I will update with pictures and questions I'm sure.

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Invisiblefastfred
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Re: nutrient agar + dry malt extract light ?? [Re: QuailOakinit]
    #18213885 - 05/04/13 03:39 PM (11 years, 17 days ago)

Quote:

whats the difference between a still air box and a glove box?




Not sure.  The term wasn't in use when I was last kicking around here.  I assume that SAB is just something new that someone made up because they didn't know it was called a GB.


-FF

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OfflineQuailOakinit
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Re: nutrient agar + dry malt extract light ?? [Re: fastfred]
    #18214065 - 05/04/13 04:10 PM (11 years, 17 days ago)

I was under the impression a glove box was a box with holes it it so gloves can enter, while a SAB has gloves mounted in the box so that there is no air flowing through the glove holes.
Either way, I think we get the point when someone says glovebox.


My design has two layers of tyvek for the arm sleeves, so i guess gases/air can still be exchanged.

When transferring tissue to my dishes yesterday, I had an alcohol lamp burning inside my SAB and it kept burning! Also did not blow up in my face :smile:

I took an over head video of me making my transfers, but I don't know if it is wise to post, or where, or if it would be helpful to anyone as I am just beginning with agar

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OfflinePussyFart
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Re: nutrient agar + dry malt extract light ?? [Re: QuailOakinit]
    #18214442 - 05/04/13 05:51 PM (11 years, 17 days ago)

Quote:

QuailOakinit said:
I was under the impression a glove box was a box with holes it it so gloves can enter, while a SAB has gloves mounted in the box so that there is no air flowing through the glove holes.



It's the exact opposite actually, a glove box has gloves attached to the box.

A SAB or still air box is better for this hobby.

The gloves should be attached to you, and you should be able to move in and out of the box to flame sterilize very easily.

Edited by PussyFart (05/04/13 05:51 PM)

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OfflineBloodKil
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Re: nutrient agar + dry malt extract light ?? [Re: PussyFart]
    #18216638 - 05/05/13 06:02 AM (11 years, 16 days ago)

nice glovebox Quail

Quote:

Notahacker420 said:
A SAB or still air box is better for this hobby.

The gloves should be attached to you, and you should be able to move in and out of the box to flame sterilize very easily.




No matter how many times I see this I still can't believe it.  The amount of air that gets pushed into the so called still air box is incredible from moving your hands in and out of it.  To account for the rush of air and subsequent particles/spores/etc... that will have entered you would have to wait a good deal of time for them to settle down for it to make much of a difference from doing this in open air.  (Not that I'm suggesting that it should be done in open air)

A proper glove box with a small opening to stick your scalpel/needle head through to sterilize should be much more effective at removing the risk of contamination.  (Or if using a source other than alcohol burner as your heat source it could be done from inside a sealed GB)

While a still air box might protect you from a particularly scuzzy dirty environment, and or sneezes/coughs it still will have a massive influx of possibly "contaminated" air every time you sterilize a tool. (Which should be quite often with what we are doing)

Edited by BloodKil (05/05/13 06:05 AM)

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OfflinePussyFart
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Re: nutrient agar + dry malt extract light ?? [Re: BloodKil]
    #18216787 - 05/05/13 07:27 AM (11 years, 16 days ago)

Quote:

BloodKil said:
nice glovebox Quail

Quote:

Notahacker420 said:
A SAB or still air box is better for this hobby.

The gloves should be attached to you, and you should be able to move in and out of the box to flame sterilize very easily.




No matter how many times I see this I still can't believe it.  The amount of air that gets pushed into the so called still air box is incredible from moving your hands in and out of it.  To account for the rush of air and subsequent particles/spores/etc... that will have entered you would have to wait a good deal of time for them to settle down for it to make much of a difference from doing this in open air



Not really, less than 30 seconds of waiting for particles to settle.



Quote:

BloodKil said:
While a still air box might protect you from a particularly scuzzy dirty environment, and or sneezes/coughs it still will have a massive influx of possibly "contaminated" air every time you sterilize a tool.



Not really, because we wait for the air to settle before going back to work.

I use tyvek sleeves in my SAB, this solves the coughing/sneezing problem.

But don't just take my word for it, do some research.

 

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OfflineBloodKil
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Re: nutrient agar + dry malt extract light ?? [Re: PussyFart]
    #18217426 - 05/05/13 11:20 AM (11 years, 16 days ago)

Quote:

Notahacker420 said:
1. Not really, because we wait for the air to settle before going back to work.

2. I use tyvek sleeves in my SAB, this solves the coughing/sneezing problem.

3. But don't just take my word for it, do some research.






1. Have fun waiting up to a couple hours (yep that's how long it can take in between you pulling your arms in and out)

2. You kinda missed my point...  The coughing and sneezing is what your SAB is taking care of.  Your tyvek sleeve only protects your goodies from the nasties on your skin.

3. Not to be rude, but you should take your own advice on this.  To work in a nuclear facility I had to go through quite a bit of training, and some of it pertained to the amount of time it took for particulates of different sizes to settle.  I do encourage you to go look some of this up for yourself, you might gain a new outlook on SAB.

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OfflinePussyFart
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Re: nutrient agar + dry malt extract light ?? [Re: BloodKil]
    #18217640 - 05/05/13 12:09 PM (11 years, 16 days ago)

This is not nuclear science, and you do not have to wait hours, just seconds, like I said.

The tyvek sleeves stop more than that.

Why would I need a new outlook when I have 99-100% success using my current methods? :lol:

Edited by PussyFart (05/05/13 12:10 PM)

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OfflineBloodKil
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Re: nutrient agar + dry malt extract light ?? [Re: PussyFart]
    #18219263 - 05/05/13 07:11 PM (11 years, 16 days ago)

Quote:

Notahacker420 said:
This is not nuclear science, and you do not have to wait hours, just seconds, like I said.

The tyvek sleeves stop more than that.

Why would I need a new outlook when I have 99-100% success using my current methods? :lol:




Face palm...  By all means continue on in ignorance, but don't tell others to do research when you are too lazy to do so yourself.  Tyvek isn't some magical magnet and  though I mentioned where I learned about about particular density, and how long it took particals (spores etc of varying sizes) to settle I was in no way implying you were dealing with nuclear materials (just common sense which your thought process is lacking)

As far as your 99-100%...  When younger I successfully used the pull out method for years on end, just because I was lucky every time (and had 100% success) doesn't mean I was practicing safe sex. 


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OfflinePussyFart
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Re: nutrient agar + dry malt extract light ?? [Re: BloodKil]
    #18219350 - 05/05/13 07:30 PM (11 years, 16 days ago)

Feel Better?

Edited by PussyFart (05/05/13 07:30 PM)

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OfflineQuailOakinit
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Re: nutrient agar + dry malt extract light ?? [Re: PussyFart]
    #18232493 - 05/08/13 12:42 PM (11 years, 13 days ago)



So here is how they look now, I will make another transfer sometime this weekend

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Invisibleanne halonium
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Re: nutrient agar + dry malt extract light ?? [Re: QuailOakinit]
    #18232555 - 05/08/13 12:52 PM (11 years, 13 days ago)

box.






agar is fert agar.


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OfflineQuailOakinit
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Re: nutrient agar + dry malt extract light ?? [Re: anne halonium]
    #18240057 - 05/09/13 08:22 PM (11 years, 12 days ago)

Here they are again! The "telephone" brand agar dishes seem to have more rizomorphic growth, but the nutrient agar dishes are still growing mycelium, fluffier but bright white. I am only really looking through the sides so I don't know if there is contamination or not.


Here are the dishes.




So, out of 50 3 sector dishes, I have 150 samples. How many would you guys recommend I transfer??? Should I not bother with the "nutrient agar" dishes since the others appear more rizomorphic??

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