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OfflineTerramoto
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Registered: 04/10/13
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First time growing shrooms
    #18193793 - 04/30/13 07:39 PM (11 years, 8 months ago)

Hey! Im going to start growing shrooms but im a bit concern about the sterilization. At first i was thinking about using a PC but my PC doesn't have a gauge, so i thought if i miss the 15 psi mark i would fail to sterilize and that i should go with steam sterilization (like on the mushroomsvideo.com). To clean the glove box i was thinking about using 1/10 bleach/water but it sterilizes or sanitizes? Could i use alcohol instead and get the same result?

Also the only rice i found that looks like brown rice was a coarse rice. On the website of the brand it doesnt say its organic but it says the rice was not processed in any way, just removed from its husk. Will this work?

Ill be using glass cups with tin foil.

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OfflineMHbound
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Re: First time growing shrooms [Re: Terramoto]
    #18193908 - 04/30/13 08:00 PM (11 years, 8 months ago)

What is the model number of the pressure cooker?

For the glovebox I just spray the fuck out of it with Lysol.


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OfflineiOmniphobia
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Re: First time growing shrooms [Re: MHbound] * 1
    #18194114 - 04/30/13 08:42 PM (11 years, 8 months ago)

The rice should work fine. Grind it finely.

Lysol bombing is useless. You're.not.creating a sterile.place.  only a still air environment.

Simply damp the inside via.spraybottle, paper towel with water.

Clean with.bleach solution if.you'd like.

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Offlinethundahoof
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Re: First time growing shrooms [Re: iOmniphobia]
    #18194301 - 04/30/13 09:15 PM (11 years, 8 months ago)

Your pc manual will tell you its psi.


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OfflineTerramoto
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Re: First time growing shrooms [Re: MHbound]
    #18194351 - 04/30/13 09:22 PM (11 years, 8 months ago)

It doesnt have a model number it looks like the one on the image it holds 2-3 gallons, this types of PC doesnt bring manual, its used for cooking it just has something that makes the noise when the vapor comes out. Im going to buy the rest of the items tomorrow and im missing bleach and alcohol, i'll do fine with just alcohol, right?


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OfflineMHbound
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Re: First time growing shrooms [Re: Terramoto]
    #18195325 - 05/01/13 02:00 AM (11 years, 8 months ago)

It has to have some markings somewhere to tell what kind it is, otherwise I don't feel safe saying you can run it without the weight...you can just buy a pressure cooker weight for it cheap but I need the maker or something off of it....its on there somewhere. at least the name.

Lysol is not pointless....You are creating a still air environment yes but its not totally sterile and its better safe than sorry to lysol the gloves the bottom sides and top....why not? especially considering he is using glass with foil.

Let it air out before you inoculate or the Lysol will flame up...Or let it settle rather don't air it out that would defeat the purpose.


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OfflineMHbound
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Re: First time growing shrooms [Re: MHbound] * 1
    #18195333 - 05/01/13 02:01 AM (11 years, 8 months ago)

Use alcohol if you want, I just use Lysol so you can cover every surface easily and know its sterile.


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OfflineTerramoto
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Re: First time growing shrooms [Re: MHbound]
    #18195432 - 05/01/13 03:03 AM (11 years, 8 months ago)

I cant find Lysol here thats why i didnt consider it, the PC as a brand
http://www.flama.pt/index.php?cat=75&item=146 , there's not much information nor manuals it just says the PC is made out of stainless steel 18/10.

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Offlineillumi.naughty87
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Re: First time growing shrooms [Re: MHbound] * 1
    #18195456 - 05/01/13 03:21 AM (11 years, 8 months ago)

No matter what you do, the SAB will never be sterile. But like someone else mentioned, sterility is not the goal of a SAB, still air is the goal. I think most people just use a bleach water solution to spray it down anyway. I hear that a cardboard box with a window works just fine, and I cant imagine someone soaking cardboard with anything.

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OfflinePete Burbington
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Re: First time growing shrooms [Re: Terramoto] * 1
    #18195467 - 05/01/13 03:27 AM (11 years, 8 months ago)

Dude, I have heard of people using pressure cookers that have only reached 10 psi and have never had any problems. By the look of that picture, I am sure you will be just fine for now.

As for lysol or a generic, if you cant find that.... then you have no business growing.

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OfflineMHbound
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Re: First time growing shrooms [Re: Pete Burbington] * 1
    #18195528 - 05/01/13 04:26 AM (11 years, 8 months ago)

I am seeing it reaches 10 PSI without anything, just put a damp rag over the hole on top to help maintain the pressure and do it a little longer than usual.

The reason 15 is important is because he is using the glasses and not jars with lids. But you can get away with it but I would cook them a little longer...

Are you doing the PF Tek or grains?

And you can get a glovebox sterile, 100%? No, but you can get it very sterile with alcohol if you have to, but where the hell are you that you can't find lysol?

anyway have any questions you can PM me.


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OfflineTerramoto
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Re: First time growing shrooms [Re: MHbound]
    #18195572 - 05/01/13 04:58 AM (11 years, 8 months ago)

When you said i should spray it with Lysol i thought that i would have to look for that specific product, its just another disinfectant im sure ill be able to find some.

Im doing PF Tek, the mushroomvideo.com guy used the steam method the video said that 100ºC would be enough to sterilize.

Thanks for your help :laugh:

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OfflineMHbound
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Re: First time growing shrooms [Re: Terramoto] * 1
    #18195937 - 05/01/13 07:57 AM (11 years, 8 months ago)

Yea you can find some no problem. A lot of people use Lysol or a disinfectant its easy to spray everything and its very effective. I spray the shit out of the entire room, surfaces and all before I start innoculating.

Any other questions just ask. Be careful with the foil lids, if you can find tyvek at the local post office putting it under the foil and wrapping a rubberband or zip tie to hold it would be very beneficial and then cover the inoculation points with some porous tape.

Good luck.


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OfflineTerramoto
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Re: First time growing shrooms [Re: MHbound]
    #18226801 - 05/07/13 11:45 AM (11 years, 8 months ago)

Hey, today i made the spore syringe this way:

desinfected the glove box with bleach/water and wait 15min
desinfected the spore recipient with 80% alcohol and let it dry
scrape the spores in
heat the syringe needle till red
threw the water in 3,4x

after i did all this i started to think about what i did wrong and i think i just killed the spores by sucking them in, when the needle was still warm? Or the first time i throw the water the needle will drop the heat?

tomorrow ill be mixing the verm+flour and sterilize using the PC, i think even if its not a 15 psi PC 90 will sterilize it +-? I saw some posts here of people saying it worked for them. I went to the pharmacies around here, guess what none sells micropore tape (i read here that it wouldnt filter spores), the only thing i was able to buy was this and this i will be cutting this in small stripes, will this work as a filter?

Edited by Terramoto (05/07/13 11:46 AM)

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OfflineTerramoto
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Re: First time growing shrooms [Re: Terramoto]
    #18228639 - 05/07/13 06:05 PM (11 years, 8 months ago)

anyone?

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OfflinecronicrMFacebook
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Re: First time growing shrooms [Re: Terramoto] * 1
    #18228744 - 05/07/13 06:27 PM (11 years, 8 months ago)

Dependsing on what psi it is but the answer is yes it will work, and your spores are fine.
I cant see the links im on my phone but im gonna say its fine as long as it wont seal your holes


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineTerramoto
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Re: First time growing shrooms [Re: cronicr]
    #18249294 - 05/11/13 04:33 PM (11 years, 8 months ago)

Ok, i've injected 8 cups with the spores, i suppose ill be able to see a small white mycellium in a week or so? Will i be able to use the cake, after harvesting, to make a liquid culture out of it? cut a piece and put in a solution, would it work?

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Re: First time growing shrooms [Re: Terramoto] * 1
    #18249465 - 05/11/13 05:21 PM (11 years, 8 months ago)

no when its spent it's spent and also covered in contams, try agar its more rewarding then lc


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinebassclef
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Re: First time growing shrooms [Re: cronicr] * 1
    #18249521 - 05/11/13 05:37 PM (11 years, 8 months ago)

place an alcohol soaked paper towel at the bottom of your SAB to catch anything that falls.. also soap and water on the walls.. best ways to keep it clean in your work area.

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OfflineTerramoto
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Re: First time growing shrooms [Re: cronicr]
    #18249754 - 05/11/13 06:39 PM (11 years, 8 months ago)

Quote:

cronicr said:
no when its spent it's spent and also covered in contams, try agar its more rewarding then lc




i've read somewhere that i could add H2O2 that it wouldnt kill the mycellium and keep it clean, what do you mean by spent? I dont fully understand mushrooms so im considering a mushroom an aglomeration of entities, if one part dies the another wouldnt simply grow? Or the whole aglomeration dies? Even if contam, wouldnt mycellium have an advantage over the other bacterias or fungi if you present it with a substract to grow? I've seen this guide where the author uses the bottom of a mushroom to make a LC.

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