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Mummitrollet
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Registered: 04/25/13
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Last seen: 8 years, 5 months
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Growing log. First try. Please comment. Want to learn as much as possible! :)
#18167925 - 04/25/13 06:17 PM (11 years, 8 months ago) |
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Hi fellow growers.
Im completely new to this forum and also growing so I thought I would keep a log about my progress, both to keep track of what I have done and for u guys to give me suggestions and help when u feel like it
Allright, I have just gotten a syringe with Amazonians and I started yesterday with cooking 3 plastic tupperware boxes, 0,5L each, 4 holes punched out in the lid and masking tape over them and a layer of alufoil, in a big boiling pot for 90min to get them sterilized. Elevating the boxes with some old lids and alufoil to get them above water.
I use as a substrate a mix of brown rice flour (BRF), verm and water. And dry verm on top of that as protection. I am basically trying to follow as much as possible the "Lets grow mushrooms" videos on youtube since it seems quite easy. We will see 
The boiling pot stood the whole night cooling down without lifting the lid. This morning I was going to inoculate them, but it did not go as planned. The holes where too small for the syringe so I had to punch out bigger holes and I somehow felt the risk for contam would be bigger by doing that so what I did was I just inoculated one of the boxes and I am going to boil the other two again tonight. To spread the risk and experiment with how careful I have to be.
So I inoculated one box. Injecting a small amount, not sure exactly how much but enough to see a small wet stream coming out in the box. In four places. I took the masking tape of and put the alufoil on again. Not sure if the masking tape should have stayed on, but lets see how it goes I did this with sterilized latex gloves and heating up the needle with a lighter for 20sek or so. This was inside a sterilized plastic box with the lid halfway on. Dont have a inoculation box, but will see if I need to get that if this goes to shit.
Now the box is inside a small plastic box, sterilized, which in turn is also inside a bigger box, also sterilized. My plan is to get a heating element in a pet store tomorrow so I can keep the temp stable and not have to heat up my entire bedroom. I have a temp and RH-gauge and plan to keep the temp at around 27celsius until fully colonized.
Any suggestions or similar experiences is gratefully accepted Will post pics soon..
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Mummitrollet
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Registered: 04/25/13
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18180671 - 04/28/13 10:24 AM (11 years, 8 months ago) |
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I now have three boxes in there.

Before the last (dry) layer of verm.
I have now a total of 5 tupperware boxes split up into: 2 in my closet. Temp under 20celsius and fluctuating during day. No light. And also 3 boxes in an inoculation chamber(?), box-inside-box with water and heater, with almost constant temp of 23-ish.
I have heard different stories about how to store the inoculated boxes so figured I would test out the different methods myself.
Thanks for reading.
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FungiFractals
Sacred Geometry



Registered: 04/06/09
Posts: 224
Last seen: 9 months, 11 days
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18180698 - 04/28/13 10:32 AM (11 years, 8 months ago) |
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incubators are not usually required while a still air box will make you a very happy person by almost guaranteeing low contam rates (i would convert it)
Edit: micropore tape comment removed. verm layer will work.
Edited by FungiFractals (04/28/13 10:35 AM)
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Mummitrollet
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: FungiFractals]
#18228249 - 05/07/13 04:40 PM (11 years, 8 months ago) |
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Yes, it seems like people do different things there. In the video I mentioned in the first post (lets grow mushrooms), they seem not to use one. But after seeing the results from my boxes, I can pretty much conclude that the closet boxes develop much slower than the ones in the incubator. I also, like u say, feel more safe in regard to contam. No sign of any bad green mold or anything. So far so great I did not even take photo of the ones in the closet, but they seem to lag a short week or so behind the others.
This was after 6 days:

14 days:

They are definitively progressing, but a little slower than I thought. Any thoughts on this? Last week the temp has been around 24celcius. And yea, I should also mention: The temp measure device are situated on top of the lid (stuck through a hole there) so I assume the true temp at the bottom of the box is a degree or more higher.
Thanks guys!
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Mummitrollet
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Registered: 04/25/13
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18239255 - 05/09/13 05:17 PM (11 years, 8 months ago) |
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Updated photos:
A few of the cultures are starting to grow out on top of the substrate. Is this bad or nothing to worry about?
Taken today, after 14 days.( The last photos of the previous post was of another box which have been growing two more days, so on 16 days now and coming strong. The ones in the closet are also slowly, sloooowly coming to ).

Just so u can compare, here is one of the two closet boxes:
 Thanks!
Edited by Mummitrollet (05/09/13 05:25 PM)
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ChillbroSwaggins

Registered: 04/01/13
Posts: 175
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18239309 - 05/09/13 05:31 PM (11 years, 8 months ago) |
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looks good to me, man
take the foil off if you have a dry verm barrier, unless the foil is the lid
Edited by ChillbroSwaggins (05/09/13 05:36 PM)
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18239335 - 05/09/13 05:36 PM (11 years, 8 months ago) |
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Quote:
Mummitrollet said: Updated photos:
A few of the cultures are starting to grow out on top of the substrate. Is this bad or nothing to worry about?
Did you wipe down the sides of your jars before adding the verm barrier on top? You can give nasties a way down to your mycel if not, like a string to follow.
Looks more to me like you have been to rough with them, making the verm barrier come out of place, and loose its purpose.
In addition, 0,25L, half your size is recommended for PF-tek cakes, 0,5 tends to take to long to finish.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: spacechildo]
#18239640 - 05/09/13 06:50 PM (11 years, 8 months ago) |
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be sure to give those things alot of time, for future refrence your jars/containers should be the same hight as width for best performance, but gl 2 you
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Mummitrollet
Stranger

Registered: 04/25/13
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Last seen: 8 years, 5 months
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: cronicr]
#18241277 - 05/10/13 01:23 AM (11 years, 8 months ago) |
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I will be removing the alufoil. Thanks.
I think u r right. I have been a bit too careless with my handling of them. I did though wipe down at the lip, but maybe because of later running of the substrate along the lip have given them an opportunity too grow to far up. I dont dare open the lid and wipe it, so I will just cross my fingers and hope the others behave better
When I inoculated the boxes I used four holes and four squirts with the syringe. But it seems like only 2 or 3 of the inoculations in each box have given results. Is this normal?
Cronicr: Thanks for the tip. Buying mason jars here in Norway turned out to be a difficult thing, so this was plan b so to speak. I did found someone (with glass lid) after my inoculation and will probably use them next time because this takes a long time I see now.
Thanks guys Will keep posting
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 6 hours, 7 minutes
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18241295 - 05/10/13 01:30 AM (11 years, 8 months ago) |
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Remember to shake the syringe between injections
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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darkdarkerdarko
Phlebotomist


Registered: 10/07/12
Posts: 104
Last seen: 5 years, 2 months
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: cronicr]
#18241369 - 05/10/13 01:58 AM (11 years, 8 months ago) |
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those are very big containers for brf cakes so itll be slower and they might also be packed too tightly by the looks of it
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darkdarkerdarko
Phlebotomist


Registered: 10/07/12
Posts: 104
Last seen: 5 years, 2 months
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18241379 - 05/10/13 02:00 AM (11 years, 8 months ago) |
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very good looking myc
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Mummitrollet
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Registered: 04/25/13
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Last seen: 8 years, 5 months
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: darkdarkerdarko]
#18242728 - 05/10/13 10:51 AM (11 years, 8 months ago) |
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Yes, I knoooow :P Will definitely be using smaller containers next time 
I tried not to push down on the verm, but maybe I did. Will be more careful next time for sure. Is it more okay to push down on the dry verm layer on top or is neither preferred?
I wonder if there is a time limit before the myc have spent too much time growing and will die or be more subjected to contam. Is shaking the containers a good idea? To spread the myc, I mean.
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Mummitrollet
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Registered: 04/25/13
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Last seen: 8 years, 5 months
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18242783 - 05/10/13 11:02 AM (11 years, 8 months ago) |
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Is there a preferred amount of dry verm layer or just fill it to the top?
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darkdarkerdarko
Phlebotomist


Registered: 10/07/12
Posts: 104
Last seen: 5 years, 2 months
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18244139 - 05/10/13 04:24 PM (11 years, 8 months ago) |
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i would not shake brf and verm. grains only.
the verm layer is only for keeping out contams and absorbing any extra moisture and should be just as loose but doesnt have to be more than 1/2 in for any container. any more is unnecessary and can be bad for gas exchange
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Mummitrollet
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Registered: 04/25/13
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Last seen: 8 years, 5 months
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: darkdarkerdarko]
#18251683 - 05/12/13 03:49 AM (11 years, 8 months ago) |
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That sounds reasonable. Thanks
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Mummitrollet
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Re: Growing log. First try. Please comment. Want to learn as much as possible! :) [Re: Mummitrollet]
#18442786 - 06/19/13 02:42 PM (11 years, 6 months ago) |
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Its been a long time now since last post. The boxes took waaay too long, and most of them had been contaminated by the time I put them in the fruit chamber. Now, I only have this one cake left from the big ones. I do have some others going on at the same time, from smaller boxes (2,5dl) which is now also in the fruiting chamber. Separately from this one since I suspect contam. What do you guys think? Cobweb?
Thanks Cant wait to post my first fruits soon!
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