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InvisibleSemilanceata
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Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Oven
    #1816382 - 08/15/03 10:38 AM (14 years, 8 months ago)

Is it possible for a poor man like myself, to do all the critical operations like inoculating, making spore syringes, agar transfers...with an oven and still being safe and contamination free ?



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Offlineshakta
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Registered: 06/03/03
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Re: Oven [Re: Semilanceata]
    #1816445 - 08/15/03 11:03 AM (14 years, 8 months ago)

It depends on who you ask. A lot of people think the oven tek is bogus.


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InvisibleOJK
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Re: Oven [Re: shakta]
    #1816481 - 08/15/03 11:15 AM (14 years, 8 months ago)

Yeah, it's pretty hit and miss, some people have had great results with an oven, some people have had terrible contam problems. A glovebox is so cheap and easy to make, I'd go with that.

:grin:


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Invisiblekoraks
Registered: 06/02/03
Posts: 25,433
Re: Oven [Re: shakta]
    #1816484 - 08/15/03 11:17 AM (14 years, 8 months ago)

The problem with ovens is that it's quite difficult to create a sterile environment inside one, and to keep that environment sterile.
I myself have never used an oven, but a simple cooking pot on a gas stove to sterilize jars and syringes and a cigarette lighter to sterilize hypodermic needles. With a little bit of care, you can work pretty much contam free with these simple things.


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InvisibleOJK
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Re: Oven [Re: koraks]
    #1816490 - 08/15/03 11:21 AM (14 years, 8 months ago)

Well yeah but for agar transfers and g2g transfers and syringe making a sterile environment is important... ovens are used because in theory the rising warm air takes contaminants with it and prevents them settling on the work area.


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Amazon Shop: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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