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masonwinston
just a guy


Registered: 06/19/13
Posts: 57
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18563549 - 07/15/13 06:53 PM (10 years, 11 months ago) |
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After being hyrdated per Violet's instructions, how long must the RGS grains sit in the strainer before I put the grain in my jar and PC?
Or would it be more appropriate to ask how dry the RGS should be?
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bassclef
Stranger

Registered: 02/09/13
Posts: 115
Last seen: 9 years, 4 months
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: masonwinston]
#18563602 - 07/15/13 07:05 PM (10 years, 11 months ago) |
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so in the end I had some decent results and average 4-5 flushes from my containers.. my last grow was a great learning experience.. I did things a few different ways and landed on a setup I really like... The RGS did great, but i don't think I'm going to continue with it.. Until I get a flow hood, i might come back to this method, but the contam rate for me doing G2G's and agar work so far without a flow hood has been not so good.
Once I get a flowhood for easier G2G work i'm going to revist this tek.
In the end I averaged about 2-3g per container over 4-5 flushes each.
I settled on Rye Berries(RR's Prep Tek) > Coir/Verm/Gypsum in 6qt shoe boxes > greenhouse
I bought a load of grass seed and I plan to use it up if and when I get some sclerotia producers.
I should note that I did modify this tek a bit and made lids with inoculation ports that i scared the plastic and used RTV Silicon. I didn't have the ability to G2G at the time and used a MS syringe.
Edited by bassclef (07/15/13 07:07 PM)
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: bassclef]
#18563682 - 07/15/13 07:22 PM (10 years, 11 months ago) |
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glad to hear ya had a degree of luck bassclef.
this tek, does require a certain element of sterile skill. ive used this tek in environments ranging from " pretty good" to " pretty bad".
it will tell ya your ambient contam level.
there are ways to get around this. increasing sterile skill... using SAB/ HEPA , and, by using slurrys/ needle innoc.
G2G , is always, just what it is. in a clean environment, its effective. it loses its value, as the environment degrades though.
once again , im most pleased with everyones results. its a paradigm grow shift, that must be seen to fully appreciate.
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ForestBellows
Stranger

Registered: 05/22/13
Posts: 83
Last seen: 9 years, 4 months
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18573953 - 07/17/13 11:18 PM (10 years, 11 months ago) |
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Anne, what is your preferred method for needle inoculation? As in, do you just lift the lid and squirt some in or do you mod the lid/body somehow?
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: ForestBellows]
#18573967 - 07/17/13 11:22 PM (10 years, 11 months ago) |
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I've inoc'd with needle or even bare syringes by squirting thru a briefly lifted lid. Of course I have that happening in sterile airflow, but a still-air-box/glovebox would work for that as well. That would be my recommendation to anyone. However, as said before, a poly-fil stuffed hole is completely reliable and allows for lid-tight sterilization as well as closed-container needle inoc.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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LungCheeseFungus
Stranger Everyday


Registered: 05/01/13
Posts: 75
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18578960 - 07/19/13 12:18 AM (10 years, 11 months ago) |
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Quote:
anne halonium said: im not sure plastic color, would have a clear advantage.
I am uncertain if it was intentional, but I did find the humor in this statement regarding clear or colored plastics. Thank you, I owe ya one!
It is good to see this thread and method continue to develop.
I am curious if a red hot MS syringe could puncture the sterilized and previously unaltered PP5 screw top container lid, inoculate, and immediately upon its withdraw, with pressure applied to the lid to provide a positive pressure environment, have tape/felt/band-aid applied, all while retaining adequate cleanliness. If you are correct, Violet, in saying that a properly loaded container will usually not require GE to achieve full colonization, then perhaps even regular tape would suffice. If there happened to be one that stalls, the lid could be cracked, I suppose.
Of course (if it works) this would be an easy adaptation for the newcomer. Maybe even at a later date the small hole could be heated with a flame and pressed to be permanently (hopefully!) sealed, once the usefulness of a SAB ect. is realized. Not quiet unmodified like you like, Violet, but close to it. Any thoughts anyone?
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: LungCheeseFungus]
#18579679 - 07/19/13 06:16 AM (10 years, 11 months ago) |
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air exchange, isnt really an issue with lose lid PP5's. ive never had one stall out.
given a choice, of altering lids, or using an SAB and clean environment, id go with clean and SAB.
i know peeps like to mod equip. the tek was sorta designed and tested, to avoid alot of that.
fewest parts, fewest materials, fewest mods, least supply.
as always, for the smaller op, anything goes, and its all about the same. for the larger op, direct load em and go, no mods, is where the tek has its advantages.
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LungCheeseFungus
Stranger Everyday


Registered: 05/01/13
Posts: 75
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18580549 - 07/19/13 11:53 AM (10 years, 11 months ago) |
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Quote:
anne halonium said: given a choice, of altering lids, or using an SAB and clean environment, id go with clean and SAB.
for the larger op, direct load em and go, no mods, is where the tek has its advantages.
I concur. Not quiet there yet, but I can see several potential benefits with this TEK, especially when scaled up. Isolation of contaminated substrates, and the overwhelming simplicity and lack of necessary supplies are not the least of which.
-------------------- If you have not found something worth dieing for, you have found nothing worth living for. The most effective barrier to knowledge, is the illusion of it. Is there any long time member here that used to have the handle "Mr. Cool" elsewhere? PM me.
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: LungCheeseFungus]
#18580561 - 07/19/13 11:58 AM (10 years, 11 months ago) |
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it was designed for the 100-300 container size scale farm. above that, its assumed one would rotate containers.
the isolation factor alone, makes a big diff to scale.
those who feel daunted by many containers, should have 2 PC's.
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LungCheeseFungus
Stranger Everyday


Registered: 05/01/13
Posts: 75
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18580665 - 07/19/13 12:30 PM (10 years, 11 months ago) |
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What do you mean by rotating the containers exactly? If you have a GH/FC capable of holding say 200 containers at any given time, then have say 400 containers total? Half fruiting, half consolidating, and swap them out as you begin to remove the least productive containers? Or maybe they all kind of run out at the same time, if they were all made in the same manner and from the same genetics?
Sorry, I'm a very curious person, especially when I recognize innovation and simplicity together.
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Trippy_Penguin



Registered: 03/18/12
Posts: 624
Last seen: 3 years, 10 months
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: LungCheeseFungus]
#18580696 - 07/19/13 12:38 PM (10 years, 11 months ago) |
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You're onto the right track. If you always have grain/seed colonizing, you won't have empty spots in your greenhouse (or at least not many) It is unlikely that they would all need to be swapped out at the same time given in a decent sized green house there would be multiple rotations.
Edited by Trippy_Penguin (07/19/13 12:45 PM)
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ghiajake
Myco-Viking


Registered: 01/10/13
Posts: 3,923
Loc: Indiana
Last seen: 2 days, 4 hours
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18580773 - 07/19/13 12:58 PM (10 years, 11 months ago) |
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Quote:
anne halonium said:
those who feel daunted by many containers, should have 2 PC's.
I got five bucks in 3 PC's. "Can't beat that with stick!" Building my flow hood in the next couple days, so all I need now is the containers. Got 4x 1pt "cakes" of undisturbed myc colony (rolled in dry verm) in fruiting now to see how that does. Once I get flow hood built I'll feel a lot more comfortable using the unmolested lids. Since I'm getting a hang on the agar now, I'm one step closer to following this correctly.
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LungCheeseFungus
Stranger Everyday


Registered: 05/01/13
Posts: 75
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: Trippy_Penguin]
#18580806 - 07/19/13 01:07 PM (10 years, 11 months ago) |
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Ahh, so some mini rotations aye? Sounds fun! Maybe if I put a lid on my head I can sneek into one of of yalls greenhouses and do a little quality control. Rotate me in!
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: Trippy_Penguin]
#18580812 - 07/19/13 01:08 PM (10 years, 11 months ago) |
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its as simplistic as this . assume 40 min per cook load, giant cooker +/- 10 PP5 per load. assume 2 PC's, and assume an assistant, and reasonable OHSA/union breaks for cigs and bathroom ....
it would take a few days to do a few hundred containers. they would not all incubate / fruit/ prosper at exactly the same speed.
therefore, as ya lose a few to contams, or grow cycle attrition, or in the case of MS , have a odd poor performer you can immediately, add new containers to replace the questionable ones.
early containers, would likely be MS, but, later containers replaced, can be clones.
its the staggering of the grow, and, the versatility, that allows for essentially a constant crop, of adjustable size.
done right, YOU call the down time, not the tek.............
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Edited by anne halonium (07/19/13 01:12 PM)
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Amelia Rose
Fungi Friend


Registered: 07/12/13
Posts: 61
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18585466 - 07/20/13 03:31 PM (10 years, 11 months ago) |
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How high can the plastic containers be stacked in the pressure cooker? Mine is 22 quart and can fit 12 if I stack 3 high, will that work or is 2 high the max?
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: Amelia Rose]
#18585728 - 07/20/13 04:34 PM (10 years, 11 months ago) |
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i have 910's. they stack 2 high.
i see no reason why ya cant stack 3 high.
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Amelia Rose
Fungi Friend


Registered: 07/12/13
Posts: 61
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18586298 - 07/20/13 06:30 PM (10 years, 11 months ago) |
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great. I only saw reference to stacking two high, and thought maybe any higher and the bottom container might squish in the heat with the weight on it.
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: Amelia Rose]
#18586562 - 07/20/13 07:21 PM (10 years, 11 months ago) |
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your pry cool as directed in the tek.
id do it.
not all cookers are equal, and not all PP5 is equal. most of the time, it all works well together.
medium , and small cookers, with good PP5 containers, ive never seen an issue.
giant cookers, and, questionable quality PP5 ? might suck.
3 stack, doesnt sound unreasonable test run with containers as you would like it, half full of water.
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Amelia Rose
Fungi Friend


Registered: 07/12/13
Posts: 61
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: anne halonium]
#18589106 - 07/21/13 09:55 AM (10 years, 11 months ago) |
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I will do a test run. Thanks for the advice. It was mentioned that it is possible to fruit with this method in tubs with no holes due to lower myco load. I would be intested in doing it this way (with lid cracked) because I haven't modified my tubs yet, and no holes would be less obvious. Do you think that as a beginner I would be successful using no hole fruiting chamber? Obviously I would be fanning/misting regularily.
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Grass Seed & Plastic, Myco Match from Heaven. How I grow mushi & the million reasons why. +Potency [Re: Amelia Rose]
#18589335 - 07/21/13 10:54 AM (10 years, 11 months ago) |
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yes , you can avoid murdering your FC's with holes. note the lack of holes in my FC's

theres no way im using a carton of 12 gauge bird shot on those.
always remember, one of the key advantages to the violet tek, is the direct approach on time and supply.
violet tek, doesnt require, drilling lids or FC's, automation, misters , heavy tube light arrays, tyvek, or polyfil, verm, perle, or animal droppings, or ,stop drop birth dunk and roll.
^if all that was REQUIRED, this tek would not exist.
the violet tek, is a tek designed , to crank out PP5 containers fast assembly line style, and, the crop is managed with most simplistic basics.
fewest parts, fewest supplies, straightest grow.
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Edited by anne halonium (07/21/13 11:23 AM)
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