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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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OfflineRohypnol
Vice Figurehead

Registered: 08/04/03
Posts: 267
Last seen: 8 years, 7 months
Amp resistance, why wouldn't this work?
    #1811433 - 08/13/03 09:21 PM (20 years, 8 months ago)

I work in a developmental genetics lab, and I was thinking about the issue of contamination, and I think that I may have a simple solution. I can easily transfect an ampacillin resistance gene into the spores right next to a omniexpressive gene. All you'd have to do is add some ampacillin (a strong antibiotic) to the growing medium, thus eliminating 99.999999% of contamination. This is really easy to do too, wouldn't take longer than a few hours.

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InvisibleTxTec
Texas home andheart breaker...

Registered: 12/19/02
Posts: 1,328
Re: Amp resistance, why wouldn't this work? [Re: Rohypnol]
    #1811441 - 08/13/03 09:24 PM (20 years, 8 months ago)

Did you figure this out with Ohms law ?


--------------------
I felt a warm warm breeze that melted metal and steele

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InvisibleLiL_KuSsH
Lost In The Trip
I'm a teapot

Registered: 05/07/03
Posts: 3,001
Deleted [Re: TxTec]
    #1811501 - 08/13/03 09:46 PM (20 years, 8 months ago)

Post deleted by administrator

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InvisibleGestaltAssault
Sam Kohui (c)

Registered: 05/25/03
Posts: 420 420 Posts!
Loc: Here-->[x]
Re: Amp resistance, why wouldn't this work? [Re: LiL_KuSsH]
    #1811537 - 08/13/03 09:56 PM (20 years, 8 months ago)

ok...well if it works...send me some of those spores...could you bioengineer p. cubensis to be more potent as well??


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i dont cultivate...i just post pictures, spread knowledge, and give advice
my brain's illegal because it contains DMT in the pineal gland, and so does your brain and everyone elses
the name's GestaltAssault not Get Us A Slut

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InvisibleLe_Canard
The Duk Abides

Registered: 05/16/03
Posts: 94,392
Loc: Earthfarm 1
Re: Amp resistance, why wouldn't this work? [Re: GestaltAssault]
    #1811543 - 08/13/03 10:00 PM (20 years, 8 months ago)

Yes, it sounds like a worthy endevour to me! Try it out and keep us posted on your results.

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InvisibleDiploidM
Cuban

Folding@home Statistics
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
Trusted Cultivator
Re: Amp resistance, why wouldn't this work? [Re: LiL_KuSsH]
    #1811562 - 08/13/03 10:05 PM (20 years, 8 months ago)

Quote:

ummmmm. what!?



I think it's a joke relating to the title of this thread, "Amp", which the originator meant as an abbreviation to the cell-wall antibiotic ampicillin. The reference to Ohm's Law interpreted "Amp" as the abbreviation to Ampere and made fun of the word "resistance" which is measured in Ohms.

By the way, anyone know why abbreviation is such a long word?

-Diploid

Edit: typo


--------------------
Republican Values:

1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.

4) We need a smaller, less-intrusive government.

Edited by Diploid (08/13/03 10:07 PM)

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OfflineSuntzu
Geek
 User Gallery

Registered: 10/14/99
Posts: 1,396
Last seen: 14 days, 13 hours
Re: Amp resistance, why wouldn't this work? [Re: Rohypnol]
    #1812749 - 08/14/03 07:57 AM (20 years, 8 months ago)

I don't quite understand.

Ampicillin resistance is inherent in fungi already, as it acts on [many] prokaryotic's biochemistry.  This would reduce it's usefulness in combating mold contamination if the transfection were successful.

Ampicillin plates will germinate spores just fine.  It's actually my antibiotic of choice because it's so cheap and available.

As far as 'easily transfecting' next to a normally active promoter, well. . .I guess we have different views of what is 'easy'.  :wink: 

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InvisibleOJK
Stranger
 User Gallery
Registered: 06/08/03
Posts: 10,629
Re: Amp resistance, why wouldn't this work? [Re: Suntzu]
    #1812860 - 08/14/03 08:52 AM (20 years, 8 months ago)

:lol:

I think Suntzu has a point... and hell, if you can use your lab for genetically modifying physcadelic mushroom spores, can't you just grow your mushrooms there? That would be a very easy way to combat contamination

:grin:

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Offlinegonzo2012
GONZO
Registered: 06/27/03
Posts: 18
Last seen: 20 years, 5 months
Re: Amp resistance, why wouldn't this work? [Re: OJK]
    #1814535 - 08/14/03 05:37 PM (20 years, 8 months ago)

how would you know this gene your'e transfecting would be expressed?? wouldn't it have to be within the omniprescent gene you referred to?? if so, what vector would you use to get it there???

i'm intrigued.... keep us informed

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