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Ravinoff
LethargicSnorting Duck
Registered: 07/15/03
Posts: 686
Last seen: 20 years, 6 months
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9 days since innoculation...
#1809663 - 08/13/03 01:59 PM (20 years, 7 months ago) |
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I innoculated 6 jars 9 days ago with GT and there are no signs of germination
I was thinking of re-innoculating half of them with pesa today, just incase they weren't viable(I got them from a shroomerite, not a vendor)
I would rather to have GTs and I was wondering if they suddenly sprouted some mycelia in the next few days if the pesa spores would be a problem?
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mikey
Stranger
Registered: 06/04/03
Posts: 548
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Re: 9 days since innoculation... [Re: Ravinoff]
#1809692 - 08/13/03 02:05 PM (20 years, 7 months ago) |
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they would fight it out, just noculate half and leave rest to wait
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Erik006
MushroomCultivator
Registered: 07/13/03
Posts: 310
Loc: Ontario
Last seen: 13 years, 8 months
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Re: 9 days since innoculation... [Re: mikey]
#1809754 - 08/13/03 02:22 PM (20 years, 7 months ago) |
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When did you innoculate them? Right after pc'ing? Or did you leave them to cool overnight?
Erik006
-------------------- At last you know what ineffable is, and what ecstacy means
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Ravinoff
LethargicSnorting Duck
Registered: 07/15/03
Posts: 686
Last seen: 20 years, 6 months
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Re: 9 days since innoculation... [Re: Erik006]
#1809805 - 08/13/03 02:31 PM (20 years, 7 months ago) |
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Some people say a minimum of 6 hours, or cool to the touch
I let them cool for an entire day just to be sure
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Erik006
MushroomCultivator
Registered: 07/13/03
Posts: 310
Loc: Ontario
Last seen: 13 years, 8 months
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Re: 9 days since innoculation... [Re: Ravinoff]
#1809819 - 08/13/03 02:35 PM (20 years, 7 months ago) |
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I know what to do, I was asking how you did it to find out if maybe you innoculated to spores when the jars were still hot, but i guess not. Are you incubating them? If yes at what temp?
Erik006
-------------------- At last you know what ineffable is, and what ecstacy means
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george castanza
Lord Of The Idiots!
Registered: 10/21/02
Posts: 8,743
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Re: 9 days since innoculation... [Re: Ravinoff]
#1809824 - 08/13/03 02:36 PM (20 years, 7 months ago) |
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what temps are you incubating at? give them 1 more week and if nothing pitch them out DO NOT re-inocculate bad jars as it is a very bad idea...fresh inoculation means fresh jars
at least thats whaqt kramer says
-------------------- KRAMER CAKES
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Ravinoff
LethargicSnorting Duck
Registered: 07/15/03
Posts: 686
Last seen: 20 years, 6 months
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Incubating at 80 - 82
And I think I'm just gonna go ahead and re-innculate, a few CC's ain't much
thanks for advice
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Erik006
MushroomCultivator
Registered: 07/13/03
Posts: 310
Loc: Ontario
Last seen: 13 years, 8 months
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>DO NOT re-inocculate Hes right, if nothing shows up in another week, fix up new jars and innoculate. Good Luck.
If youre innoculating at low temps it will take a longer time, so then a week isnt too bad.
Erik006
-------------------- At last you know what ineffable is, and what ecstacy means
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DanKnugget
Mysterious Traveller
Registered: 07/15/03
Posts: 1,074
Loc: On a boat
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Re: 9 days since innoculation... [Re: Erik006]
#1809902 - 08/13/03 02:54 PM (20 years, 7 months ago) |
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Now why wouldn't you innoculate sterile jars? If they haven't grown anything, and the substrate is still wet, it works just fine! I would go for it, don't waste perfectly good substrate.
-------------------- My 3 proverbs: 1. School is for people who don't know stuff. 2. Jobs are for losers. 3. Reading is for people with nothing better to do.
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EthanEdwards
In which Dorisgets her oats
Registered: 06/04/03
Posts: 183
Last seen: 15 years, 5 months
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Re: 9 days since innoculation... [Re: DanKnugget]
#1809965 - 08/13/03 03:11 PM (20 years, 7 months ago) |
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you cant see all contamination, what looks like perfectly normal jars could be contamimated
-------------------- Everybody must give something back for something they got.- Bob Dylan
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Flipmcneil
Commander Cool
Registered: 07/20/03
Posts: 116
Loc: Where the wild things are
Last seen: 18 years, 6 months
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Re: 9 days since innoculation... [Re: EthanEdwards]
#1809987 - 08/13/03 03:16 PM (20 years, 7 months ago) |
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What kind of substrate are you using? PF cake or WBS, etc.. I know where those syringes came from and the spores contained within colonized 1 pint WBS jars in 7 days here.
-------------------- "He who makes a beast of himself gets rid of the pain of being a man" Dr. Johnson
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Ravinoff
LethargicSnorting Duck
Registered: 07/15/03
Posts: 686
Last seen: 20 years, 6 months
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Re: 9 days since innoculation... [Re: Flipmcneil]
#1809993 - 08/13/03 03:17 PM (20 years, 7 months ago) |
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PF cake, in a 1 pint jar And the syringes you used were from the same batch you sent me?
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MycoCakeEater
Old Hand
Registered: 06/16/03
Posts: 1,572
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Re: 9 days since innoculation... [Re: Ravinoff]
#1810000 - 08/13/03 03:20 PM (20 years, 7 months ago) |
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I agree dont re-innoc. If they didnt germ the first time in that jar they prob wont do it again. Just save your spores and innoc some more. Except this time do a karo syrup liquid culture tek. It only takes 1cc and gaves me 10 syringes full of mycelium. A great great investment! If you have any ques just PM and Ill help you with it.
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Flipmcneil
Commander Cool
Registered: 07/20/03
Posts: 116
Loc: Where the wild things are
Last seen: 18 years, 6 months
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Re: 9 days since innoculation... [Re: Ravinoff]
#1810001 - 08/13/03 03:21 PM (20 years, 7 months ago) |
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Yes they were from the same batch. From what I have seen 1 pint pf cakes take awhile to colonize.
-------------------- "He who makes a beast of himself gets rid of the pain of being a man" Dr. Johnson
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Flipmcneil
Commander Cool
Registered: 07/20/03
Posts: 116
Loc: Where the wild things are
Last seen: 18 years, 6 months
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Re: 9 days since innoculation... [Re: Ravinoff]
#1810002 - 08/13/03 03:21 PM (20 years, 7 months ago) |
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Yes they were from the same batch. From what I have seen 1 pint pf cakes take awhile to colonize.
-------------------- "He who makes a beast of himself gets rid of the pain of being a man" Dr. Johnson
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Ravinoff
LethargicSnorting Duck
Registered: 07/15/03
Posts: 686
Last seen: 20 years, 6 months
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Re: 9 days since innoculation... [Re: Flipmcneil]
#1810027 - 08/13/03 03:27 PM (20 years, 7 months ago) |
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Ok, I'll just let 'em sit then
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 2 months
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Re: 9 days since innoculation... [Re: DanKnugget]
#1810213 - 08/13/03 04:11 PM (20 years, 7 months ago) |
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Quote:
DanKnugget said: Now why wouldn't you innoculate sterile jars? If they haven't grown anything, and the substrate is still wet, it works just fine! I would go for it, don't waste perfectly good substrate.
not me.
chances are if nothing grew, something wasn't right with it. and chances also are that the substrate is probably cheaper and more disposable than the spores. why take the risk? if something fails at the first hurdle, start over, says monkey.
-------------------- buh
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 2 months
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Re: 9 days since innoculation... [Re: LiL_KuSsH]
#1810247 - 08/13/03 04:20 PM (20 years, 7 months ago) |
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and lil kush rules right back atcha!
-------------------- buh
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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MycoCakeEater
Old Hand
Registered: 06/16/03
Posts: 1,572
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Re: 9 days since innoculation... [Re: shirley knott]
#1810350 - 08/13/03 04:36 PM (20 years, 7 months ago) |
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You both rule! yeah shirley thanks for being a great admin!
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 2 months
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Re: 9 days since innoculation... [Re: MycoCakeEater]
#1810373 - 08/13/03 04:40 PM (20 years, 7 months ago) |
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admin? promotion! you rock and rule too - hell, we all do!
/me proposes everyone form a circle and pat the person in front of you on the back.
-------------------- buh
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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MycoCakeEater
Old Hand
Registered: 06/16/03
Posts: 1,572
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Re: 9 days since innoculation... [Re: LiL_KuSsH]
#1810415 - 08/13/03 04:49 PM (20 years, 7 months ago) |
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HAAHAH thanks for the correction. Your a great MOD then.
BTW: Lil_kussh has only one testcle and its name is Quarter Pounder. Just wanted everyone to know.
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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MycoCakeEater
Old Hand
Registered: 06/16/03
Posts: 1,572
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Re: 9 days since innoculation... [Re: LiL_KuSsH]
#1810459 - 08/13/03 04:57 PM (20 years, 7 months ago) |
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Yes now I only have to reveal the other 2 of Kussh's deadly secrets!
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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MycoCakeEater
Old Hand
Registered: 06/16/03
Posts: 1,572
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Re: 9 days since innoculation... [Re: LiL_KuSsH]
#1810502 - 08/13/03 05:04 PM (20 years, 7 months ago) |
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No even better..... Do you still remeber the time with the 3 legged dog and a broomstick?!
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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OJK
Stranger
Registered: 06/08/03
Posts: 10,629
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Re: 9 days since innoculation... [Re: LiL_KuSsH]
#1810568 - 08/13/03 05:17 PM (20 years, 7 months ago) |
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LMFAO
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: OJK]
#1810598 - 08/13/03 05:24 PM (20 years, 7 months ago) |
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Post deleted by administrator
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MycoCakeEater
Old Hand
Registered: 06/16/03
Posts: 1,572
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Re: 9 days since innoculation... [Re: LiL_KuSsH]
#1810880 - 08/13/03 06:53 PM (20 years, 7 months ago) |
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Im so goth I'm dead. Just a funny t-shirt Ive seen. Wait what was this post about?
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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MycoCakeEater
Old Hand
Registered: 06/16/03
Posts: 1,572
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Re: 9 days since innoculation... [Re: LiL_KuSsH]
#1810942 - 08/13/03 07:09 PM (20 years, 7 months ago) |
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I know....
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