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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 5 months
9 days since innoculation...
    #1809663 - 08/13/03 03:59 PM (13 years, 6 months ago)

I innoculated 6 jars 9 days ago with GT and there are no signs of germination

I was thinking of re-innoculating half of them with pesa today, just incase they weren't viable(I got them from a shroomerite, not a vendor)

I would rather to have GTs and I was wondering if they suddenly sprouted some mycelia in the next few days if the pesa spores would be a problem?


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Invisiblemikey
Stranger

Registered: 06/04/03
Posts: 548
Re: 9 days since innoculation... [Re: Ravinoff]
    #1809692 - 08/13/03 04:05 PM (13 years, 6 months ago)

they would fight it out, just noculate half and leave rest to wait


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OfflineErik006
MushroomCultivator

Registered: 07/13/03
Posts: 310
Loc: Ontario
Last seen: 6 years, 7 months
Re: 9 days since innoculation... [Re: mikey]
    #1809754 - 08/13/03 04:22 PM (13 years, 6 months ago)

When did you innoculate them? Right after pc'ing? Or did you leave them to cool overnight?

Erik006


--------------------
At last you know what ineffable is, and what ecstacy means


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 5 months
Re: 9 days since innoculation... [Re: Erik006]
    #1809805 - 08/13/03 04:31 PM (13 years, 6 months ago)

Some people say a minimum of 6 hours, or cool to the touch

I let them cool for an entire day just to be sure


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OfflineErik006
MushroomCultivator

Registered: 07/13/03
Posts: 310
Loc: Ontario
Last seen: 6 years, 7 months
Re: 9 days since innoculation... [Re: Ravinoff]
    #1809819 - 08/13/03 04:35 PM (13 years, 6 months ago)

I know what to do, I was asking how you did it to find out if maybe you innoculated to spores when the jars were still hot, but i guess not. Are you incubating them? If yes at what temp?

Erik006


--------------------
At last you know what ineffable is, and what ecstacy means


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Invisiblegeorge castanzaM
Lord Of The Idiots!
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Registered: 10/21/02
Posts: 7,291
Trusted Cultivator
Re: 9 days since innoculation... [Re: Ravinoff]
    #1809824 - 08/13/03 04:36 PM (13 years, 6 months ago)

what temps are you incubating at?
give them 1 more week and if nothing pitch them out DO NOT re-inocculate bad jars as it is a very bad idea...fresh inoculation means fresh jars

at least thats whaqt kramer says


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 5 months
Re: 9 days since innoculation... [Re: george castanza]
    #1809851 - 08/13/03 04:40 PM (13 years, 6 months ago)

Incubating at 80 - 82

And I think I'm just gonna go ahead and re-innculate, a few CC's ain't much

thanks for advice


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OfflineErik006
MushroomCultivator

Registered: 07/13/03
Posts: 310
Loc: Ontario
Last seen: 6 years, 7 months
Re: 9 days since innoculation... [Re: george castanza]
    #1809854 - 08/13/03 04:41 PM (13 years, 6 months ago)

>DO NOT re-inocculate
Hes right, if nothing shows up in another week, fix up new jars and innoculate. Good Luck.

If youre innoculating at low temps it will take a longer time, so then a week isnt too bad.

Erik006


--------------------
At last you know what ineffable is, and what ecstacy means


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InvisibleDanKnugget
Mysterious Traveller
Male

Registered: 07/15/03
Posts: 1,074
Loc: On a boat
Re: 9 days since innoculation... [Re: Erik006]
    #1809902 - 08/13/03 04:54 PM (13 years, 6 months ago)

Now why wouldn't you innoculate sterile jars? If they haven't grown anything, and the substrate is still wet, it works just fine! I would go for it, don't waste perfectly good substrate.


--------------------
My 3 proverbs:
1. School is for people who don't know stuff.
2. Jobs are for losers.
3. Reading is for people with nothing better to do.


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OfflineEthanEdwards
In which Dorisgets her oats

Registered: 06/04/03
Posts: 183
Last seen: 8 years, 5 months
Re: 9 days since innoculation... [Re: DanKnugget]
    #1809965 - 08/13/03 05:11 PM (13 years, 6 months ago)

you cant see all contamination, what looks like perfectly normal jars could be contamimated


--------------------
Everybody must give something back for something they got.- Bob Dylan


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OfflineFlipmcneil
Commander Cool

Registered: 07/20/03
Posts: 116
Loc: Where the wild things are
Last seen: 11 years, 5 months
Re: 9 days since innoculation... [Re: EthanEdwards]
    #1809987 - 08/13/03 05:16 PM (13 years, 6 months ago)

What kind of substrate are you using? PF cake or WBS, etc..
I know where those syringes came from and the spores contained within colonized 1 pint WBS jars in 7 days here.


--------------------

"He who makes a beast of himself gets rid of the pain of being a man"
Dr. Johnson


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 5 months
Re: 9 days since innoculation... [Re: Flipmcneil]
    #1809993 - 08/13/03 05:17 PM (13 years, 6 months ago)

PF cake, in a 1 pint jar

And the syringes you used were from the same batch you sent me?


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InvisibleMycoCakeEater
Old Hand
Male User Gallery

Registered: 06/16/03
Posts: 1,572
Re: 9 days since innoculation... [Re: Ravinoff]
    #1810000 - 08/13/03 05:20 PM (13 years, 6 months ago)

I agree dont re-innoc. If they didnt germ the first time in that jar they prob wont do it again. Just save your spores and innoc some more. Except this time do a karo syrup liquid culture tek. It only takes 1cc and gaves me 10 syringes full of mycelium. A great great investment! If you have any ques just PM and Ill help you with it.


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OfflineFlipmcneil
Commander Cool

Registered: 07/20/03
Posts: 116
Loc: Where the wild things are
Last seen: 11 years, 5 months
Re: 9 days since innoculation... [Re: Ravinoff]
    #1810001 - 08/13/03 05:21 PM (13 years, 6 months ago)

Yes they were from the same batch. From what I have seen 1 pint pf cakes take awhile to colonize.


--------------------

"He who makes a beast of himself gets rid of the pain of being a man"
Dr. Johnson


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OfflineFlipmcneil
Commander Cool

Registered: 07/20/03
Posts: 116
Loc: Where the wild things are
Last seen: 11 years, 5 months
Re: 9 days since innoculation... [Re: Ravinoff]
    #1810002 - 08/13/03 05:21 PM (13 years, 6 months ago)

Yes they were from the same batch. From what I have seen 1 pint pf cakes take awhile to colonize.


--------------------

"He who makes a beast of himself gets rid of the pain of being a man"
Dr. Johnson


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 5 months
Re: 9 days since innoculation... [Re: Flipmcneil]
    #1810027 - 08/13/03 05:27 PM (13 years, 6 months ago)

Ok, I'll just let 'em sit then


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: Ravinoff]
    #1810107 - 08/13/03 05:49 PM (13 years, 6 months ago)

Post deleted by administrator


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Offlineshirley knott
not my real name
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Registered: 11/11/02
Posts: 9,105
Loc: London Flag
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Re: 9 days since innoculation... [Re: DanKnugget]
    #1810213 - 08/13/03 06:11 PM (13 years, 6 months ago)

Quote:

DanKnugget said:
Now why wouldn't you innoculate sterile jars? If they haven't grown anything, and the substrate is still wet, it works just fine! I would go for it, don't waste perfectly good substrate.


not me.

chances are if nothing grew, something wasn't right with it. and chances also are that the substrate is probably cheaper and more disposable than the spores. why take the risk? if something fails at the first hurdle, start over, says monkey.


--------------------
buh


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: shirley knott]
    #1810223 - 08/13/03 06:13 PM (13 years, 6 months ago)

Post deleted by administrator


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Offlineshirley knott
not my real name
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Registered: 11/11/02
Posts: 9,105
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Last seen: 1 month, 18 days
Trusted Cultivator
Re: 9 days since innoculation... [Re: LiL_KuSsH]
    #1810247 - 08/13/03 06:20 PM (13 years, 6 months ago)

:grin:

and lil kush rules right back atcha! :thumbup: 


--------------------
buh


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