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blindingleaf said:
I wish my serve safe certification protected me against con tams.

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Whippy said:
One thing frank is guilty of is

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frank has alluded in other threads to less than clean conditions
if he wants to stack tubs in filth, fine.
it reprehensible to suggest as much to noobs and the gullible.

no serious pro would accept franks cavalier bio handling.
the community should realize, his sanitary skills.
it has every bearing, on his threads.

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Whippy said:
OR are you aware of the point,
and are simply using it out of context?

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That sounds like anne Never pass up a chance to be obtuse. Just like most "bulkers" don't use "corn", many avoid "poo", and of late very few use a "bucket" these days. But to hear anne tell it we

"fill our crystal masons with corn
dreaming of a trich free world
While we boil our turds
the noobs bucket holds sway
while look over our harvests of trich"

or something to that effect.

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anne halonium said:
^thats the frank/ annie difference.

i see cleanliness, proper bio handling,
and pathogen awareness,
as central to any fungi grow thread.

its apparent, frank is not least concerned.

one is left wondering.

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I'm not left wondering.

I'm just curious why we'd need to practice sterile procedure, pathogen awareness, etc., for a shroomery thread?  

Are you afraid it's going to contaminate shroomery?
RR

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FrankHorrigan said:
You don't even have to be sterile with a colonized grain. I used to make isolates from grains that fell to the floor of my SAB during G2G. Just pick it up when you are done with your work and place it on a clean dish

The myc in the grain is so aggressive that it will outrun contams on the agar.

Just make sure to take a transfer away from the grain itself before making wedges for inoculation.

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This is a similar concept to cloning a pin on agar, which RR demonstrates in his videos. The pin is obviously not sterile because it was grown in open air.

Idky Anne is making such a fuss .... I'm sure the inside of your SAB is relatively clean. It's not like it fell in the fucking toilet lol.

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Novanity1 said:
Idky Anne is making such a fuss

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cronicr said:
Not all her fault, were all the result of a ms inoculation, ya win some ya lose some

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but some of us got bigger syringes than others

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Pastywhyte said:

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cronicr said:
Not all her fault, were all the result of a ms inoculation, ya win some ya lose some

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but some of us got bigger syringes than others

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SpitballJedi said:

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Pastywhyte said:

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cronicr said:
Not all her fault, were all the result of a ms inoculation, ya win some ya lose some

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but some of us got bigger syringes than others

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Yeah. MS syringes are not 100% clean and she is the contam. The ignore button is our digital agar.

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cronicr said:
Not all her fault, were all the result of a ms inoculation, ya win some ya lose some

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Not me. I was concieved isolated sperm cell on ovarian agar.

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Novanity1 said:
This is a similar concept to cloning a pin on agar, which RR demonstrates in his videos. The pin is obviously not sterile because it was grown in open air.

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Idky Anne is making such a fuss .... I'm sure the inside of your SAB is relatively clean. It's not like it fell in the fucking toilet lol.

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I think I might drop a colonized grain in toilet water and place it on agar.

Then I'm going to grow a 10-tub crop from that culture and spread the love to everyone I know.

I'll call it the "toilet water dunk tek."  Just for anne

I'd place bets on the initial plate coming out alright with no visible contams. Colonized grains are aggressive as hell and goddamn if I don't keep a clean toilet

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TheCyndicate said:
Just for the record . I'm the third generation to live in the house I'm in .  Like frank stated above ..... Proper technique will always prevail . I have had my far share of contamination from the mean green to pink lipstick , black mold, and all kinds of bacterial contamination, and it always discouraged me . But once I realized that is was my sterile tech. I took a step back and reviewed everything I was doing .  I took the proper measures to assure myself that I was using proper sterile tech. And since then no contamination . I use a home made flow hood to scrub the air 24/7 24"X24" in size and a 24"X12" FP  flow hood to work in front of . I use a SAB when doing transfers that are contaminated . In short practice your sterile tech and you will come out on top

Cyn

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QFT.... As if it needs to be