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Farmerwhite
Cultivation student



Registered: 02/03/13
Posts: 161
Last seen: 4 years, 10 months
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How can you tell when a spawn bag is fully colonized?
#18077045 - 04/08/13 02:04 PM (10 years, 9 months ago) |
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This is probably a dumb question but I have a 3 lb grain spawn bag that has been colonizing for a month and looks done to me but I don't know for sure. I can provide pictures if I need to. Thank you for your time.
FW
-------------------- I'm here to learn from the best Objects only have meaning that you put upon them
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18077087 - 04/08/13 02:11 PM (10 years, 9 months ago) |
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When the surface and all the sides are completely covered in mycelium
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PsilyPhily
Stranger


Registered: 02/07/13
Posts: 560
Last seen: 10 years, 3 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18077091 - 04/08/13 02:13 PM (10 years, 9 months ago) |
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If its a full white block you are all good. Why are you unsure?
Phil.
-------------------- "If a drug (or technique or process) were ever to be discovered which would consistently produce a plus four experience in all human beings, it is conceivable that it would signal the ultimate evolution, and perhaps the end, of the human experiment." — Alexander Shulgin, PiHKAL, 1991 "Well Shulgin, you clearly haven't tried salvia 150X extract, good effort though."
Phil.
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Premedman1
Assistant to the insistent



Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: How can you tell when a spawn bag is fully colonized? [Re: PsilyPhily]
#18077183 - 04/08/13 02:28 PM (10 years, 9 months ago) |
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Break it up while it's in the bag. If the inside is colonized, it's ready to spawn to your bulk substrate. If not, put it back into colonization conditions and wait another week or until full colonization.
-------------------- Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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Farmerwhite
Cultivation student



Registered: 02/03/13
Posts: 161
Last seen: 4 years, 10 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: Premedman1]
#18080527 - 04/09/13 05:36 AM (10 years, 9 months ago) |
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I've already broken it 2 tokes during colonization and I've read any more than that is detrimental and I am just skeptical about one side, the bottom.
-------------------- I'm here to learn from the best Objects only have meaning that you put upon them
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PsilyPhily
Stranger


Registered: 02/07/13
Posts: 560
Last seen: 10 years, 3 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18080544 - 04/09/13 05:56 AM (10 years, 9 months ago) |
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Ok yer I'v had that before, I believe it is caused by condensation dripping down the bag and causing the mycelium to not be able to colonize.
I have spawned a bag like that and had no problems but that being said it would be better if it would colonize it..
Do you incubate at all?
Phil.
-------------------- "If a drug (or technique or process) were ever to be discovered which would consistently produce a plus four experience in all human beings, it is conceivable that it would signal the ultimate evolution, and perhaps the end, of the human experiment." — Alexander Shulgin, PiHKAL, 1991 "Well Shulgin, you clearly haven't tried salvia 150X extract, good effort though."
Phil.
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Farmerwhite
Cultivation student



Registered: 02/03/13
Posts: 161
Last seen: 4 years, 10 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: PsilyPhily]
#18080745 - 04/09/13 08:08 AM (10 years, 9 months ago) |
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I was worried it was condensation, there are visible spots of liquid with a slight yellow tint throughout the bottom. I've read the yellow is caused by metabolites, is this true?
Yes I was forced to make a small TiT to keep the temps stable. I live in a city with a very unstable climate and the temps would range between 60 and 75 in my house so my colonization was moving at snail's pace but as soon as I put my bags in the TiT the mycelium started ripping through the grain.
-------------------- I'm here to learn from the best Objects only have meaning that you put upon them
Edited by Farmerwhite (04/09/13 08:12 AM)
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PsilyPhily
Stranger


Registered: 02/07/13
Posts: 560
Last seen: 10 years, 3 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18080921 - 04/09/13 08:59 AM (10 years, 9 months ago) |
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It's not something to worry about.. It will always happen.
That yellow is probably metabolites yer but it could also be bacteria so I would give it a bit longer because you have mixed the bag up so if it is contams they could be inside the bag so waiting could make them grow out until visible..
Metabolites could also be a sign of contams but is most likely to be stress from heat or something..
I have to incubate too.. It sucks but as long as the temps stay in range then its all good, Just be careful when fruiting because condensation in your FC could be all the RH being sucked out..
I would wait for a TC to chime in as I don't have tonnes of experience with spawn bags.. Only started with them recently tbh..
Phil.
-------------------- "If a drug (or technique or process) were ever to be discovered which would consistently produce a plus four experience in all human beings, it is conceivable that it would signal the ultimate evolution, and perhaps the end, of the human experiment." — Alexander Shulgin, PiHKAL, 1991 "Well Shulgin, you clearly haven't tried salvia 150X extract, good effort though."
Phil.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18080945 - 04/09/13 09:08 AM (10 years, 9 months ago) |
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Quote:
Farmerwhite said: I've already broken it 2 tokes during colonization and I've read any more than that is detrimental and I am just skeptical about one side, the bottom.

That bag looks fine, use it. The best way to test if it's good or not is to break it up when you are preparing to spawn it, open the bag and then smell it. It should smell like fresh mushrooms of the species you are growing  Next time, make sure you trap a good pocket of air inside the bag (in your SAB or in front of your flowhood, obviously). Your myc is probably a little oxygen starved.
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Farmerwhite
Cultivation student



Registered: 02/03/13
Posts: 161
Last seen: 4 years, 10 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: FrankHorrigan]
#18081411 - 04/09/13 11:25 AM (10 years, 9 months ago) |
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Thank you so much everyone for all your help and advice, as you can tell im totally new to all of this. i will be spawning it to a Monotub later today.I don't think I'll be working with spawn bags anymore after this. I ordered all the supplies for my next grow and some supplies to clone a nice healthy fruit from this grow, so pumped!
This is off topic but has any had success with a stipe biopsy transferred to LC for cloning?
-------------------- I'm here to learn from the best Objects only have meaning that you put upon them
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PsychoWorth
Angry Bird

Registered: 03/19/13
Posts: 5
Last seen: 10 years, 9 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18081448 - 04/09/13 11:36 AM (10 years, 9 months ago) |
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FarmerWhite:
Your spawn bag looks perfectly fine. Break it up and toss it in.
Congratulations on your first Spawn Bag cultivation and hopefully bulk grow
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"All your WBS are belong to us!"
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PsychoWorth
Angry Bird

Registered: 03/19/13
Posts: 5
Last seen: 10 years, 9 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18081453 - 04/09/13 11:38 AM (10 years, 9 months ago) |
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Quote:
Farmerwhite said: Thank you so much everyone for all your help and advice, as you can tell im totally new to all of this. i will be spawning it to a Monotub later today.I don't think I'll be working with spawn bags anymore after this. I ordered all the supplies for my next grow and some supplies to clone a nice healthy fruit from this grow, so pumped!
This is off topic but has any had success with a stipe biopsy transferred to LC for cloning?
Here Farmer, enjoy... http://www.shroomery.org/forums/showflat.php/Number/5874305#5874305
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"All your WBS are belong to us!"
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: How can you tell when a spawn bag is fully colonized? [Re: PsychoWorth]
#18081495 - 04/09/13 11:47 AM (10 years, 9 months ago) |
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Quote:
PsychoWorth said:
Quote:
Farmerwhite said:
This is off topic but has any had success with a stipe biopsy transferred to LC for cloning?
Here Farmer, enjoy... http://www.shroomery.org/forums/showflat.php/Number/5874305#5874305
That's an awful tek 
Here is how to take a proper clone OP.
I rarely ever get a contam on a clone this way
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PsychoWorth
Angry Bird

Registered: 03/19/13
Posts: 5
Last seen: 10 years, 9 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: FrankHorrigan]
#18081507 - 04/09/13 11:49 AM (10 years, 9 months ago) |
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Quote:
FrankHorrigan said:
Quote:
PsychoWorth said:
Quote:
Farmerwhite said:
This is off topic but has any had success with a stipe biopsy transferred to LC for cloning?
Here Farmer, enjoy... http://www.shroomery.org/forums/showflat.php/Number/5874305#5874305
That's an awful tek 
Here is how to take a proper clone OP.
I rarely ever get a contam on a clone this way 
Haha, well thank you for sharing then!
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"All your WBS are belong to us!"
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Farmerwhite
Cultivation student



Registered: 02/03/13
Posts: 161
Last seen: 4 years, 10 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: PsychoWorth]
#18081509 - 04/09/13 11:49 AM (10 years, 9 months ago) |
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Thank you Pyschoworth! I've read that thread many times but I have received mixed opinions and experiences from the TEK but it's worth a shot!
-------------------- I'm here to learn from the best Objects only have meaning that you put upon them
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PsychoWorth
Angry Bird

Registered: 03/19/13
Posts: 5
Last seen: 10 years, 9 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: PsychoWorth]
#18081514 - 04/09/13 11:51 AM (10 years, 9 months ago) |
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But i believe he asked specifically about LC because he DOESNT have Agar...
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"All your WBS are belong to us!"
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PsychoWorth
Angry Bird

Registered: 03/19/13
Posts: 5
Last seen: 10 years, 9 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18081520 - 04/09/13 11:52 AM (10 years, 9 months ago) |
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Quote:
Farmerwhite said: Thank you Pyschoworth! I've read that thread many times but I have received mixed opinions and experiences from the TEK but it's worth a shot!
Ah, sorry i couldn't be of more help then. FrankHorrigan might know of an LC Clone Tek... But it's obvious he prefers Agar, as he should.
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"All your WBS are belong to us!"
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: How can you tell when a spawn bag is fully colonized? [Re: PsychoWorth]
#18081540 - 04/09/13 12:00 PM (10 years, 9 months ago) |
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Quote:
PsychoWorth said:
Quote:
Farmerwhite said: Thank you Pyschoworth! I've read that thread many times but I have received mixed opinions and experiences from the TEK but it's worth a shot!
Ah, sorry i couldn't be of more help then. FrankHorrigan might know of an LC Clone Tek... But it's obvious he prefers Agar, as he should.
You can actually just use LC in place of the agar. It's a little more risky but I've gotten away with it before I started using agar. Just follow the tek and use two blades- one to cut the tissue from the center of the stem and the other to help manipulate it before you put it into your LC. You could just use one blade but sometimes the tissue likes to get tangled around the end of it and it's difficult to scrape off. Make sure to flame sterilize both blades before performing the procedure. Bring everything inside the still air box or in front of the hood. Wipe down the recieving jar with an iso soaked paper towel and loosen the lid of the jar a bit. Perform the excision of the tissue. Open the lid of the LC jar for as little time as possible. If need be you can wipe the loose tissue on the inner edge of the jar then close the lid tightly and tilt it carefully to submerge the tissue in the LC.
I think I'll add this information to the tek
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Farmerwhite
Cultivation student



Registered: 02/03/13
Posts: 161
Last seen: 4 years, 10 months
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Re: How can you tell when a spawn bag is fully colonized? [Re: FrankHorrigan]
#18081631 - 04/09/13 12:23 PM (10 years, 9 months ago) |
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So you are saying cloning to LC is possibly there is just a higher risk of contam and when it contams it's hard to tell. I can get agar no problem but I want to make sure this hobby is something I want to stick with before I get into the advanced stuff, I just think mycelium and mushrooms are intriguing and being able to expand my mind with the fruits of my labor is just an added bonus
-------------------- I'm here to learn from the best Objects only have meaning that you put upon them
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: How can you tell when a spawn bag is fully colonized? [Re: Farmerwhite]
#18081696 - 04/09/13 12:41 PM (10 years, 9 months ago) |
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Quote:
Farmerwhite said: So you are saying cloning to LC is possibly there is just a higher risk of contam and when it contams it's hard to tell.
Yep
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