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wood chip
Stranger

Registered: 02/22/09
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Last seen: 10 years, 5 months
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mycelium running mortality
#18050240 - 04/03/13 12:32 AM (11 years, 1 month ago) |
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I am wondering how long can you transfer young mycelium to new plates without strain degeneration.
Is there any good reason not to just keep the fresh mycelium running on petri plates and when a grain master is needed still continue the culture on new petri plates?
Can a cutting from a high quality strain continue growing for a year without problems by transferring the culture every 7 days to a new plate when the mycelium looks young and has made few cell divisions?
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
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Re: mycelium running mortality [Re: wood chip]
#18053422 - 04/03/13 04:49 PM (11 years, 1 month ago) |
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Quote:
by transferring the culture every 7 days to a new plate when the mycelium looks young and has made few cell divisions?
huh? what?
I think a "culture tube" is used to keep the "master culture"; every time you need a new "master grain jar" you pull from that tube...so one process, if I understand this correctly, would be:
1) Isolate culture on petri
2) transfer culture to a couple "master culture slants/tubes" (make a "master grain jar" while you're at it)
2) Let the transferred cultures in the slants colonize
3) Refrigerate slants/tubes till the next time you need a "master grain jar"
4) pull culture from slant to make master grain jars and when your slant is a bout to run out, transfer to petri to insure there's no contamination and then transfer to new slant...
also, bump, even though this is probably a dead horse topic for most, I find there's no harm in going over and reviewing the fundamental techniques of mycology 
of course, you could use a petri dish to keep your master culture, but I dunno about the life span / contamination risk; culture tubes have screw-cap tops
edit: the "transfer every 7 days" method sounds like it would age the culture faster... refrigeration stalls growth
Edited by MonkeyJesusFresco (04/03/13 04:51 PM)
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Terry M
Stranger in a Strange Land



Registered: 06/18/10
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Loc: Rhode Island
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Freshly growing mycelium isn't necessarily young. It can be very old and close to senescence.
Think of it this way. Everybody's got fresh cells constantly growing in their bodies, even very old people. But those newly divided cells in the elderly will have degenerated DNA. Same for all organisms.
The only thing that refreshes the DNA is sexual reproduction. In mushrooms, that means starting from spores. To keep a particular strain young and effectively prevent it from aging, you've got to resort to drastic means, like freezing to liquid nitrogen temperatures.
-------------------- Liberté, égalité, humidité.
Edited by Terry M (04/04/13 01:55 PM)
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wood chip
Stranger

Registered: 02/22/09
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Re: mycelium running mortality [Re: Terry M]
#18057571 - 04/04/13 01:09 PM (11 years, 1 month ago) |
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I am talking about limiting exponential growth in order to maintain vigorous growing mycelium (yet outside the exponential cell divisions that occur in spawn or bulk substrates) on hand for grain masters. This question is for advanced growers and can not be answered without experience.
It is a question of batch vs continuous culture.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 5 days, 23 hours
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Re: mycelium running mortality [Re: wood chip]
#18057720 - 04/04/13 01:38 PM (11 years, 1 month ago) |
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search: senescence
-------------------- LAGM v 2.024 - endo cabendo
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Terry M
Stranger in a Strange Land



Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 3 months
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Re: mycelium running mortality [Re: wood chip]
#18057729 - 04/04/13 01:40 PM (11 years, 1 month ago) |
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Quote:
wood chip said: I am talking about limiting exponential growth in order to maintain vigorous growing mycelium (yet outside the exponential cell divisions that occur in spawn or bulk substrates) on hand for grain masters. This question is for advanced growers and can not be answered without experience.
It is a question of batch vs continuous culture.
I have two degrees in biology. Cell division is cell division. This is really basic stuff.
-------------------- Liberté, égalité, humidité.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 5 days, 23 hours
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Re: mycelium running mortality [Re: Terry M]
#18057770 - 04/04/13 01:49 PM (11 years, 1 month ago) |
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Quote:
Terry M said:
Quote:
wood chip said: I am talking about limiting exponential growth in order to maintain vigorous growing mycelium (yet outside the exponential cell divisions that occur in spawn or bulk substrates) on hand for grain masters. This question is for advanced growers and can not be answered without experience.
It is a question of batch vs continuous culture.
I have two degrees in biology. Cell division is cell division. This is really basic stuff.
-------------------- LAGM v 2.024 - endo cabendo
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wood chip
Stranger

Registered: 02/22/09
Posts: 210
Last seen: 10 years, 5 months
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Degrees in biology have nothing to do with empirical experience growing mushrooms or the subject of this post. If you have nothing to contribute please don't waste time posting irrelevant information
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Terry M
Stranger in a Strange Land



Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 3 months
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Re: mycelium running mortality [Re: wood chip]
#18062160 - 04/05/13 11:54 AM (11 years, 1 month ago) |
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Quote:
wood chip said: Degrees in biology have nothing to do with empirical experience growing mushrooms or the subject of this post. If you have nothing to contribute please don't waste time posting irrelevant information
You are wasting all of our time if you don't understand enough elementary biology to know that your question is nonsense.
Moderator, please have the mercy to close this thread. For one thing, it's not Advanced Mycology.
-------------------- Liberté, égalité, humidité.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 5 days, 23 hours
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Re: mycelium running mortality [Re: Terry M]
#18062375 - 04/05/13 12:43 PM (11 years, 1 month ago) |
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Quote:
Terry M said:
Moderator, please have the mercy to close this thread. For one thing, it's not Advanced Mycology.
-------------------- LAGM v 2.024 - endo cabendo
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