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Offlineflow
outlaw immortal
Registered: 11/20/02
Posts: 496
Last seen: 7 years, 10 months
liquid mycelium
    #1804264 - 08/12/03 10:04 AM (19 years, 4 months ago)

could you take freshly grown shroom, put it in a blender with sterilized water and h202, and draw up into syringe and innoc? would this be a big contam risk?


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InvisibleGestaltAssault
Sam Kohui (c)

Registered: 05/26/03
Posts: 420 420 Posts!
Loc: Here-->[x]
Re: liquid mycelium [Re: flow]
    #1804278 - 08/12/03 10:07 AM (19 years, 4 months ago)

big contam risk...if mushrooms could reproduce like that then they wouldn't drop spores....


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i dont cultivate...i just post pictures, spread knowledge, and give advice
my brain's illegal because it contains DMT in the pineal gland, and so does your brain and everyone elses
the name's GestaltAssault not Get Us A Slut


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Offlineflow
outlaw immortal
Registered: 11/20/02
Posts: 496
Last seen: 7 years, 10 months
Re: liquid mycelium [Re: GestaltAssault]
    #1804290 - 08/12/03 10:12 AM (19 years, 4 months ago)

umm, they can reproduce like taht, its basically cloning.


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OfflinePsilocybin_monkey
Shroomer

Registered: 06/20/03
Posts: 1,340
Loc: Dragon's Den
Last seen: 11 years, 1 month
Re: liquid mycelium [Re: GestaltAssault]
    #1804297 - 08/12/03 10:14 AM (19 years, 4 months ago)

NO but you can take a 100% colonized cake and put it into a sterlized blender with sterilized water and make a slurry with it and they you can you use this to inoculate substrate or chug it down hahah

the needle of the syringe would be way to small to suck up any of the fresh mushroom blended or slurry so even if it would work it still wouldn't work :smile:


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OfflinePsilocybin_monkey
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Registered: 06/20/03
Posts: 1,340
Loc: Dragon's Den
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Re: liquid mycelium [Re: Psilocybin_monkey]
    #1804299 - 08/12/03 10:15 AM (19 years, 4 months ago)

the only part of a mushroom that you can clone is where the cap meets the stem and up(so a very small part)


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Offlineflow
outlaw immortal
Registered: 11/20/02
Posts: 496
Last seen: 7 years, 10 months
Re: liquid mycelium [Re: Psilocybin_monkey]
    #1804305 - 08/12/03 10:17 AM (19 years, 4 months ago)

how stterile would this have to be, if the blender was just washed and wiped down with alcohol would this be enough? would a generous amount of h202 help?


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OfflinePsilocybin_monkey
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Registered: 06/20/03
Posts: 1,340
Loc: Dragon's Den
Last seen: 11 years, 1 month
Re: liquid mycelium [Re: flow]
    #1804318 - 08/12/03 10:20 AM (19 years, 4 months ago)

I would wash it and then put boiling water in it and blend the water for awhile then if you want you can wipe it down with alcohol

or wipe it down with alcohol(after washed) then put the boiling water and blend it


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InvisibleGestaltAssault
Sam Kohui (c)

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Re: liquid mycelium [Re: Psilocybin_monkey]
    #1804345 - 08/12/03 10:29 AM (19 years, 4 months ago)

there's a difference between liquid mycelium and tissue culture...you were asking about liquid mycelium...

this link will explain liquid mycelium  http://www.shroomery.org/index/par/23545

and  this link will explain tissue cultures  http://www.shroomery.org/index/par/23544

how dare you question my 30 mushroom rating authority :lol: 


--------------------

i dont cultivate...i just post pictures, spread knowledge, and give advice
my brain's illegal because it contains DMT in the pineal gland, and so does your brain and everyone elses
the name's GestaltAssault not Get Us A Slut


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Offlineflow
outlaw immortal
Registered: 11/20/02
Posts: 496
Last seen: 7 years, 10 months
Re: liquid mycelium [Re: Psilocybin_monkey]
    #1804346 - 08/12/03 10:30 AM (19 years, 4 months ago)

i have some colonized rye and will try this later, thanks.
oh, one more, how much rye should i use for say 1 liter of water?
i would thik taht it wouldn't take that much.


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Offlineflow
outlaw immortal
Registered: 11/20/02
Posts: 496
Last seen: 7 years, 10 months
Re: liquid mycelium [Re: flow]
    #1804350 - 08/12/03 10:31 AM (19 years, 4 months ago)

oh, and how much h202 should i add, if at all?


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OfflinePsilocybin_monkey
Shroomer

Registered: 06/20/03
Posts: 1,340
Loc: Dragon's Den
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Re: liquid mycelium [Re: GestaltAssault]
    #1804383 - 08/12/03 10:53 AM (19 years, 4 months ago)

Quote:

GestaltAssault said:
how dare you question my 30 mushroom rating authority :lol:   




*the shroom god has spoken*


I don't think anybody will disagree with those kinda ratings hahaha :thumbup: 


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: liquid mycelium [Re: flow]
    #1804732 - 08/12/03 02:16 PM (19 years, 4 months ago)

don't mess with the blender tec.. too much hassle .
instead makeup some dextrose, or karo water .
add 1 ml. 3% h2o2 to 100 ml. liquid .
then all you need is a little tiny piece of live tissue, and incubate for 5 days or so .
cap or stem, doesn't matter .
i have done this in the open air .
i even had one jar that ants got into somehow, and it still was a viable culture .
lol, george just lucky i guess .


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InvisibleStarter
Stranger
 User Gallery

Registered: 05/16/03
Posts: 1,148
Loc: Australia
Re: liquid mycelium [Re: deanofmean]
    #1804936 - 08/12/03 05:30 PM (19 years, 3 months ago)

Actually, I inoculated 1 PF cake out of 5 from direct stipe tissue into a 4% dextrose (with H202) solution immediately to the PF glasses.

Now one took off and worked. The other four didn't, despite waiting 7 days. I decided to multispore inoculate the other four and they came good (now cased).

From that half-arse play about, I believe the tiny pieces of stipe tissue actually cultured in the injected solution on the inside of the glass and then moved to the BRF. I had noted with a 10x lens that mycelum was growing initially on the inside glass wall, not in the substrate. Not that I suggest doing this bodgy caper as a matter of course ha!

But for what it's worth, I cut up the clone PF cake into small pieces and tossed it into 3 jars of millet. They're now taking off. Meanwhile the clone dextrose solution has grown on. If I had the bytes left (only 46kb left for 24hrs lol) I'd upload the pics.

flow, give it a go, you never know your luck but with a 20% result in my effort...umm I think you're better off growing it out in a dextrose solution first.


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Offlinelemunhed
The hustler'shustler

Registered: 05/13/03
Posts: 775
Loc: Ur moms house
Last seen: 17 years, 4 months
Re: liquid mycelium [Re: Starter]
    #1805990 - 08/12/03 11:14 PM (19 years, 3 months ago)

You can clond other parts of tissue than that exact spot. It's alot easier to grow a grain jar of popcorn or rye and then put that in with some water and shake it hard.... the water rips the mycelium off the corn/rye and gives the thickest most beautiful mycelium syringes ever. Go check out "easy mycelium innoculation technique" on the shroomery.


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You're pompeius, aren't you? I'll make a pompeian of you unless you hold your toungue!

-Tiberius Claudius Nero


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