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barong
Nada


Registered: 07/24/11
Posts: 666
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Temperatures for Pouring Agar
#18038210 - 03/31/13 04:40 PM (11 years, 1 month ago) |
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Hi.
When pouring agar from glass bottles to petri's, i'm finding it difficult as its too hot to hold. I have read that there's a minimum temperature in which the agar solution is still liquid, but how does this translate to how many hours I should leave in the PC before it becomes unusable? Ambient room temp will be approx 77 degreesF.
Im about to do a batch now, and considering three hours after power off. Too much?
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PussyFart
Retired Cultivation Extrodinaire



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Re: Temperatures for Pouring Agar [Re: barong]
#18038216 - 03/31/13 04:41 PM (11 years, 1 month ago) |
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Wait until the agar cools to the point where you do not need heat protection to hold it, then pour away.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Jeff
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Re: Temperatures for Pouring Agar [Re: PussyFart]
#18038321 - 03/31/13 04:58 PM (11 years, 1 month ago) |
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I leave it in the pc until the side of the cooker is comfortable to touch. Just did a batch tonight, though I didn't time it. I would say it takes about 2.5 hours to get to the right temp.
-------------------- Myco-tek
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PussyFart
Retired Cultivation Extrodinaire



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Re: Temperatures for Pouring Agar [Re: Jeff]
#18038451 - 03/31/13 05:23 PM (11 years, 1 month ago) |
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Quote:
Jeff said: I would say it takes about 2.5 hours to get to the right temp.
This all depends on the amount of water in the PC.
I take the agar out after 2 hours, just like RR does in his videos.
If I leave the agar in the PC, 4 hours later it will still burn you.
It all depends, but what is clear, is that if you wait until you need no heat protection to hold it, you will be good.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Jeff
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Re: Temperatures for Pouring Agar [Re: PussyFart]
#18038475 - 03/31/13 05:27 PM (11 years, 1 month ago) |
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True with my AA, not so with the presto. When I run a single jar or anything small I use the presto. It's a cheap POS but works well for smaller things.
-------------------- Myco-tek
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barong
Nada


Registered: 07/24/11
Posts: 666
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Re: Temperatures for Pouring Agar [Re: Jeff]
#18040227 - 03/31/13 11:01 PM (11 years, 1 month ago) |
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Cheers guys. I popped the lid off after it naturally depressurised (with the weight on) and after a couple of hours it was good to go. Slightly too long, as it started to set at the bottom, but a good shake fixed that.
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drake89
Mushroom Magnate


Registered: 06/26/11
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Re: Temperatures for Pouring Agar [Re: barong]
#18041424 - 04/01/13 07:33 AM (11 years, 1 month ago) |
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as a former cook, I have heat tempered hands. So I can basically pour it as soon as the stopper drops on my PC.
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thiotimoline
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Registered: 12/01/12
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Re: Temperatures for Pouring Agar [Re: drake89]
#18041879 - 04/01/13 10:47 AM (11 years, 1 month ago) |
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It's still best to let it cool some before pouring, since a large temperature difference between the inside and outside of the dish leads to condensation. Agar solidifies around 100F.
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OICU812
NC Tree Farm owner


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Re: Temperatures for Pouring Agar [Re: thiotimoline]
#18044001 - 04/01/13 09:15 PM (11 years, 1 month ago) |
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I wrap a sanitized, quadruple-folded paper towel around my jar and pour at about 56C (132.8F).
-------------------- -------------- "Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin "Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin ----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Re: Temperatures for Pouring Agar [Re: OICU812]
#18044084 - 04/01/13 09:37 PM (11 years, 1 month ago) |
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Quote:
OICU812 said: I wrap a sanitized, quadruple-folded paper towel around my jar and pour at about 56C (132.8F).
Do your dishes get a lot of condensation?
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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OICU812
NC Tree Farm owner


Registered: 11/06/11
Posts: 1,064
Loc: Foothills of NC
Last seen: 3 years, 7 months
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Re: Temperatures for Pouring Agar [Re: PussyFart]
#18045099 - 04/02/13 05:04 AM (11 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
OICU812 said: I wrap a sanitized, quadruple-folded paper towel around my jar and pour at about 56C (132.8F).
Do your dishes get a lot of condensation?
I stack the dishes and cover them with the plastic slipcase they came in. Sometimes the top one will condense moisture, but I will use it first and simply dump the moisture before inoculation.
I don't think there would be a problem pouring a few degrees cooler if you only have one jar to pour. I usually have 5 or 6 jars to pour, each making 20 plates, so by the time I get to the last jar, the temperature is quite a bit cooler.
-------------------- -------------- "Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin "Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin ----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson
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