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QT3BFLEE
ส้


Registered: 02/17/13
Posts: 744
Loc: Murica
Last seen: 5 years, 8 months
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Bacteria cultures?
#18006542 - 03/25/13 12:14 AM (11 years, 1 month ago) |
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I was wondering how one would go about acquiring isolated bacterial strains for the purpose of exploring symbiotic colonies?
Like isolated actinomycetes for various psychoactive/edible fungi, and acetobacter for yeasties.
Mucho gracias por adelantado
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Strata
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Registered: 03/23/13
Posts: 16
Loc: UK
Last seen: 11 years, 28 days
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Re: Bacteria cultures? [Re: QT3BFLEE]
#18006850 - 03/25/13 02:18 AM (11 years, 1 month ago) |
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I think you'd need a lot of plates and a fresh wild specimen. I'd start by taking stem base pieces of mycelium with a little substrate on them and let them grow out onto PDA, then as a back up technique blender some of the same material and streak out various dilutions. Look for colonies that are growing close to mycelium and don't show a zone of inhibition and transfer these to a plate of clean spore grown mycelium from your target mushroom.
I reckon it'd be a mammoth task - the number of simply commensal or just plain contaminating species will be high and you won't have the advantage of being able to use antibiotics. You'll also have to show that an isolate doesn't simply grow alongside the mushrooms but also improves their performance. A microscope and basic staining set might be a really useful tool.
Well worth a try though. A guy at work used to isolate symbionts from orchids - I'll see if I can get some tips at coffee tomorrow.
I got my acetobacter ( vinegar mother in fact ) by leaving a bit of wine vinegar exposed to the air and not worrying about flies getting it it - once it started to cloud I transferred it to cider and now have great thick mats of the stuff making me vinegar.
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QT3BFLEE
ส้


Registered: 02/17/13
Posts: 744
Loc: Murica
Last seen: 5 years, 8 months
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Re: Bacteria cultures? [Re: Strata]
#18007148 - 03/25/13 04:54 AM (11 years, 1 month ago) |
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I wonder if a wild type would be necessary or if simply starting a bed would be sufficient. I can't imagine that nature would not pick up the slack, especially after an extended duration, but perhaps my understanding is faulty.
Interesting about the white vinegar -> acetobacter production!
Good luck on your biolumes, hope we get some pictures
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Strata
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Registered: 03/23/13
Posts: 16
Loc: UK
Last seen: 11 years, 28 days
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Re: Bacteria cultures? [Re: QT3BFLEE]
#18009151 - 03/25/13 03:02 PM (11 years, 1 month ago) |
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Lazy language on my part - I agree, any established outdoor bed would do the job. It might be an idea for the first few rounds not to do this anywhere near your main cultures, you might catch something nasty.
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Strata
Stranger
Registered: 03/23/13
Posts: 16
Loc: UK
Last seen: 11 years, 28 days
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Re: Bacteria cultures? [Re: Strata]
#18018602 - 03/27/13 11:34 AM (11 years, 1 month ago) |
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Spoke to the guy at work but their method would probably be a route to madness - stems were cut on the microtome, examined under the microscope and any organisms growing within the tissue excised and transferred to agar. Unless you're used to hours down the microscope and have a set of micromanipulators it's not remotely practical. You'd also need a 'scope with DIC which would set you back a bit.
A variant on the streaking out method that would be to plate out serial dilutions onto multi well plates, harder to examine but you could fit 24 samples in the footprint of two petris.
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