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Offlinemike-ologist
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whistling jars...
    #18006202 - 03/24/13 10:18 PM (11 years, 9 months ago)

two of my self healing injection port rye jars were whistling upon leaving the pc. are they still good or are they garbage?


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OfflineMycoGeo
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Re: whistling jars... [Re: mike-ologist]
    #18006215 - 03/24/13 10:21 PM (11 years, 9 months ago)

where exactly were they whistling from?

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Offlinemike-ologist
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Re: whistling jars... [Re: MycoGeo]
    #18006221 - 03/24/13 10:24 PM (11 years, 9 months ago)

it seemed to me it was from the injection ports :/


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InvisibleIntelligentxfruit
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Re: whistling jars... [Re: mike-ologist]
    #18006306 - 03/24/13 10:47 PM (11 years, 9 months ago)

The seal might have broken...which would be strange
but just look it over carefully, making sure the silicone is still intact.

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OfflineBaby_Hitler
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Re: whistling jars... [Re: mike-ologist]
    #18006325 - 03/24/13 10:53 PM (11 years, 9 months ago)

You might want to let them cool down slowly in the PC next time.


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OfflineMycoGeo
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Re: whistling jars... [Re: Baby_Hitler]
    #18006365 - 03/24/13 11:08 PM (11 years, 9 months ago)

The whistling is obviously pressure releasing from inside the jars (you knew that) The fact that it is whistling means that the leak is relatively small... When the pressure subsides the silicon should recover and re-seal that opening.

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OfflineBaby_Hitler
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Re: whistling jars... [Re: MycoGeo]
    #18006390 - 03/24/13 11:18 PM (11 years, 9 months ago)

If the jars are whistling after being taken out of the PC, it's from air being sucked in.


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OfflinePostmanJoe
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Re: whistling jars... [Re: Baby_Hitler]
    #18006430 - 03/24/13 11:31 PM (11 years, 9 months ago)

I've had this happen. Upon very close inspection I found that the port I made had a sort of air tunnel running through it. Just a vein of air that I hadn't managed to get out while applying the silicone and the ends of the tunnel were too weak to withstand the pressure.

I say tunnel but of course we're talking about a tiny scale. Regardless, those jars contaminated. It has nothing to due with letting them cool in the pressure cooker. Granted, leaving them in there would allow them to exchange a much less contaminated air than if you took them out fast and thus would lower the risk of contamination.

However, since that air tunnel exists, all it takes is a slight tempurature change to unequalize the pressure in the jar and exchange unclean air once outside the pressure cooker. The worst ones I've had will leak out of the top where they've been injected after they've been shaked.

My advice, open them up, wash them out, start again. Oh, and for some reason I've had less luck with trying to apply the silicone in a circular motion and more with just trying to slightly over-apply it  and then smooth it out as needed.

The other thing to watch for is if they bubble from the bottom of the jar repeatedly after they're done.

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Offlinemike-ologist
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Re: whistling jars... [Re: PostmanJoe]
    #18006460 - 03/24/13 11:39 PM (11 years, 9 months ago)

this is all awesome info guys thanks a million :smile: i looked at my lids and decided that i shouldnt have had some cheap liquorstore moonshine before trying to make the ports haha user error all the way. i plan on using only jars that are properly sealed. the rest ge stripped and refinnishe so to speak :smile:


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InvisibleMushroomNSwiss
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Re: whistling jars... [Re: mike-ologist]
    #18006780 - 03/25/13 01:55 AM (11 years, 9 months ago)

This doesn't make any sense. How are you getting pressure in your jars? There should be a 3/8 inch hole in the lid also for gas exchange, with tyvek or some sort of filter material over it. Which would not allow the jar to build up any type of pressure.


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OfflinePostmanJoe
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Re: whistling jars... [Re: MushroomNSwiss]
    #18006853 - 03/25/13 02:18 AM (11 years, 9 months ago)

I don't bother with that. I just make an injection port with RTV paste and once the LC is cooled I stick an empty syringe into the port stuffed with cotton that's moderately soaked in alcohol to release the vacuum that's created inside the jar by it cooling while sealed.

I've had good luck with it for quite a few batches of liquid culture. The only jars I've ever had contaminate on me were ones that had poorly made seals to begin with.

A poor RTV seal won't stand up to that. I guess it's not the most popular method but it works for me and saves me some effort in making the jars.

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OfflineBaby_Hitler
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Re: whistling jars... [Re: MushroomNSwiss]
    #18006859 - 03/25/13 02:20 AM (11 years, 9 months ago)

It would if it were cooling off very quickly.

When they first come out of the PC, if you take them out as soon as the pin drops and you can open it, there is very little, if any air in the jar, it has all been displaced by water vapor. As the water cools and condenses, it shrinks to, like, 1% of it's original volume, and sucks in the entire volume of the jar minus the volume of the grain.

A filter patch would restrict airflow less than Tyvek, but you still don't want unsterile air being sucked rapidly through the patch. It's not designed to work like that.

If you let it cool slowly in the PC, it will absorb some moisture back from the vapor , and eventually air as it replaces the vapor inside the PC. Either way, it still has to suck in the same volume of air.


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InvisibleIntelligentxfruit
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Re: whistling jars... [Re: Baby_Hitler]
    #18006956 - 03/25/13 02:57 AM (11 years, 9 months ago)

Quote:

Baby_Hitler said:
It would if it were cooling off very quickly.

When they first come out of the PC, if you take them out as soon as the pin drops and you can open it, there is very little, if any air in the jar, it has all been displaced by water vapor. As the water cools and condenses, it shrinks to, like, 1% of it's original volume, and sucks in the entire volume of the jar minus the volume of the grain.

A filter patch would restrict airflow less than Tyvek, but you still don't want unsterile air being sucked rapidly through the patch. It's not designed to work like that.

If you let it cool slowly in the PC, it will absorb some moisture back from the vapor , and eventually air as it replaces the vapor inside the PC. Either way, it still has to suck in the same volume of air.




And thats why we wait until the PC cools to room temperate :thumbup:
Well said

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Offlineveda_sticks
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Re: whistling jars... [Re: Intelligentxfruit]
    #18007054 - 03/25/13 03:55 AM (11 years, 9 months ago)

equalise the pressure with a syringe that has alcohol soaked cotton inside it? remember alcohol is not a steriliser, it merely sanitises.

If you want to fill a syringe with sterile air, flame the needle red hot and while keeping the needle red hot slow draw in air.


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