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Invisibleb plus

Registered: 10/13/12
Posts: 928
removed
    #17996345 - 03/22/13 06:58 PM (11 years, 1 month ago)

removed.


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:mushroom2:-Reishi Grow-:mushroom2:
:mushroom2:-Grey Oyster Grow-:mushroom2:

Edited by b plus (04/05/13 09:42 AM)

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OfflineRiparianZoneJunky
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Re: -B plus' agar work thread- [Re: b plus]
    #17996722 - 03/22/13 08:30 PM (11 years, 1 month ago)

Nice duder.  You should do mini bulk test runs of each isolate, get the little 5x5x3 ziploc containers from the grocery store, knock up pint jars of grain with a wedge from each iso, then put the pint jar in with some pastuerized Hpoo,

let them colonize and fruit them all alongside each other.  Clone the best looking fruit of the best performer.  I'm not sure how you expect to pick the best performer based on pinning on agar.  I guess if something is putting out huge pins from agar you could clone the pins and run them through a test, but you should really test each iso side by side.  I'm not sure pinning on agar is indicative that it will be the best overall when you put it on a substrate.  :shrug:


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RZJ's Tea Tek
RZJ's Tradelist

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Invisibleb plus

Registered: 10/13/12
Posts: 928
Re: -B plus' agar work thread- [Re: RiparianZoneJunky]
    #17996767 - 03/22/13 08:43 PM (11 years, 1 month ago)

.


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:mushroom2:-Reishi Grow-:mushroom2:
:mushroom2:-Grey Oyster Grow-:mushroom2:

Edited by b plus (04/05/13 09:42 AM)

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Offlinejjhitman
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Re: -B plus' agar work thread- [Re: b plus]
    #17997365 - 03/22/13 11:24 PM (11 years, 1 month ago)

:thumbup: nice!  Im curious about you taking a wedge from the middle of the b+ and cam?  Ive cloned and isolated but im relly curious into mixing strains to come up with super fast monster.  Any thoughts on a hybrid?


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Put water in a cup, it becomes the cup.  Put water in a tea pot, it becomes the tea pot.  Water can crash or water can flow.  Be water my friend. 


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OfflinePussyFart
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Re: -B plus' agar work thread- [Re: jjhitman]
    #17997675 - 03/23/13 01:17 AM (11 years, 1 month ago)

transfer the pins.

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Offlinejjhitman
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Re: -B plus' agar work thread- [Re: PussyFart]
    #17997752 - 03/23/13 02:01 AM (11 years, 1 month ago)

I agree.  The pin would be the faster fruiting isolation if there is such a thing as isolating it even more.?


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Put water in a cup, it becomes the cup.  Put water in a tea pot, it becomes the tea pot.  Water can crash or water can flow.  Be water my friend. 


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OfflinePussyFart
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Re: -B plus' agar work thread- [Re: jjhitman]
    #17997767 - 03/23/13 02:11 AM (11 years, 1 month ago)

Quote:

jjhitman said:
I agree.  The pin would be the faster fruiting isolation if there is such a thing as isolating it even more.?



If it's from MS, then yes, it would most likely still need isolating.

b plus, are these single sector isolates that these are fruiting from, or are there more than a single set of genetics on each dish?

Looks like different genetics to me, I say transfer some pins to new dishes and isolate until there is no sectoring.

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Invisibleb plus

Registered: 10/13/12
Posts: 928
Re: -B plus' agar work thread- [Re: PussyFart]
    #17998287 - 03/23/13 07:53 AM (11 years, 1 month ago)

Quote:

jjhitman said:
:thumbup: nice!  Im curious about you taking a wedge from the middle of the b  and cam?  Ive cloned and isolated but im relly curious into mixing strains to come up with super fast monster.  Any thoughts on a hybrid?




Well if you notice the plate, the different "srains" refuse to converge. A barrier of little to no growth forms between the two 'strains' of colliding mycelium. I've observed this with a large majority of different cubensis strains. For whatever reason, they are not compatible with one another, and refuse to converge into a "hybrid" sector.

I've tried to create my own "hybrid" strain with many different cubensis varieties. My hope's are that I can discover two different strains that will happily converge and form new 'hybrid' sectors that may possibly contain morphological traits from both parent strains.

Quote:

Notahacker420 said:

b plus, are these single sector isolates that these are fruiting from, or are there more than a single set of genetics on each dish?

Looks like different genetics to me, I say transfer some pins to new dishes and isolate until there is no sectoring.




Right you are. The Cambodian/B+ plate was nocked with a peice of grain from MS grain jars. All the rest of the fruiting plates were nocked with either invitro pins (from ms brf cake) or from tissue samples taken from mature fruits (from ms brf cakes). So I'm pretty much dealing with a mixing pool of genetics on each plate. I'm sure the genetic variance has been drastically reduced, however there are defiantly multiple sectors emerging from the invitro Ecuador pins. The "m" strain plates that were nocked with tissue clone may be the only isolates, but I'm not sure.

I figured transfering the pins would once again drastically reduce the genetic variance by propagating only the fruit producing mycelium from a multi-genetic plate.


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:mushroom2:-Reishi Grow-:mushroom2:
:mushroom2:-Grey Oyster Grow-:mushroom2:

Edited by b plus (03/23/13 07:54 AM)

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OfflinePussyFart
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Re: -B plus' agar work thread- [Re: b plus]
    #17998353 - 03/23/13 08:24 AM (11 years, 1 month ago)

Quote:

b plus said:
I figured transfering the pins would once again drastically reduce the genetic variance by propagating only the fruit producing mycelium from a multi-genetic plate.



It will, but there most likely will still be sectors, but a lot less.

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Invisibleb plus

Registered: 10/13/12
Posts: 928
Re: -B plus' agar work thread- [Re: PussyFart]
    #17998447 - 03/23/13 09:05 AM (11 years, 1 month ago)

Quote:


It will, but there most likely will still be sectors, but a lot less.




Exactly what I was thinking. I'm going to transfer both a single pin, and a small section of surrounding myc. I'll eventually grow both out to study results. I'll repeat the same experiment with every plate. My guess is that the pins will prevail, however we'll have to wait and see :shrug:. I really appreciate everyone's feedback:thumbup:


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:mushroom2:-Reishi Grow-:mushroom2:
:mushroom2:-Grey Oyster Grow-:mushroom2:

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OfflinePussyFart
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Re: -B plus' agar work thread- [Re: b plus]
    #17998654 - 03/23/13 10:14 AM (11 years, 1 month ago)

A couple of suggestions for the future:

Never let your agar plates fully colonize(I know what you were doing here) when making transfers.

Always take from the leading edge of the mycellim if you can, not the middle of the culture.

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Offlinemycofever
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Re: -B plus' agar work thread- [Re: PussyFart]
    #17998898 - 03/23/13 11:20 AM (11 years, 1 month ago)

Hey B-Plus Good job. When you have isolated your best you can send me a little sample to play with. :smile: Really though good job. I want to see your flushes/fruits from your end isolates.


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Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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Offlinejjhitman
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Re: -B plus' agar work thread- [Re: PussyFart]
    #17999104 - 03/23/13 12:10 PM (11 years, 1 month ago)

Brilliant!    I wonder if the strains are only compatible if they are a match of the same silocybin or psilocin?  Kinda like black and whites mating vs human and monkey mating?  I dont know what b+, cam, brazilian exe.. are psilocybin or psilocin right off hand?


--------------------
Put water in a cup, it becomes the cup.  Put water in a tea pot, it becomes the tea pot.  Water can crash or water can flow.  Be water my friend. 


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Offlinejjhitman
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Re: -B plus' agar work thread- [Re: jjhitman]
    #17999201 - 03/23/13 12:35 PM (11 years, 1 month ago)

Its got me thinking..... take one monokaryotic mycilium from a single spore from one strain and take another single spore (monokaryotic from another strain and try to creat a dikcryotic with the two.  The should spawn of some dikcyotic that may have dna from both.  psilocybin and psilocin has nothing to do with it.  Considering one turns in to the other after being ingested
...


--------------------
Put water in a cup, it becomes the cup.  Put water in a tea pot, it becomes the tea pot.  Water can crash or water can flow.  Be water my friend. 


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Offlinejjhitman
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Re: -B plus' agar work thread- [Re: jjhitman]
    #17999222 - 03/23/13 12:39 PM (11 years, 1 month ago)

I wish I had a microscope now.    :takingnotes:


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Put water in a cup, it becomes the cup.  Put water in a tea pot, it becomes the tea pot.  Water can crash or water can flow.  Be water my friend. 


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Invisibleb plus

Registered: 10/13/12
Posts: 928
Re: -B plus' agar work thread- [Re: PussyFart]
    #17999408 - 03/23/13 01:32 PM (11 years, 1 month ago)

Quote:

jjhitman said:
I wonder if the strains are only compatible if they are a match of the same silocybin or psilocin?  Kinda like black and whites mating vs human and monkey mating?




:facepalm:....A black person and a white person in separate populations have just as much genetic variation as two black people from one population....

Quote:

jjhitman said:
Its got me thinking..... take one monokaryotic mycilium from a single spore from one strain and take another single spore (monokaryotic from another strain and try to creat a dikcryotic with the two.  The should spawn of some dikcyotic that may have dna from both.  psilocybin and psilocin has nothing to do with it.  Considering one turns in to the other after being ingested
...




When a spore germinate, a haploid strand of mycelium called a hypha emanates from the germ pore. The hypha continues to grow and branches and becomes a mycelial network. When two sexually complementary hyphal networks intercept one another and make contact, cell walls separating the two hyphal systems dissolve and cytoplasmic and genetic materials are exchanged. The resulting mycelium is 'binucleate' and 'dikaryotic'. This means each cell has two nuclei and a full complement of chromosomes. With few exceptions, only mated (dikaryotic) mycelia is fertile and capable of producing fruitbodies. Typically dikaryotic myclium is more vigorous then unmated, monokaryotic mycelia. -straight out of Staments book.

Everything that you've fruited before has been from dikaryotic mycelium with geneitcs from two parent spores. There's possibilities were two dikaryotic mycelial networks can also grow together, exchanging genetic material and form a new "strain". This is what I'm trying to do with different strains. It's called Anastomosis. So far, I've been pretty unsuccessful. I could in theory do this by crossing two hyphal networks from single spores taken from two different strains. But I have no microscope or the tools to do such a thing:shrug:. That would be pretty fucking interesting though.


Quote:

Notahacker420 said:
Never let your agar plates fully colonize(I know what you were doing here) when making transfers. Always take from the leading edge of the mycellim if you can, not the middle of the culture.




:takingnotes:


--------------------
:mushroom2:-Reishi Grow-:mushroom2:
:mushroom2:-Grey Oyster Grow-:mushroom2:

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Offlinejjhitman
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Re: -B plus' agar work thread- *DELETED* *DELETED* [Re: b plus]
    #17999480 - 03/23/13 01:52 PM (11 years, 1 month ago)

Post deleted by jjhitman

Reason for deletion: Not worth it.



--------------------
Put water in a cup, it becomes the cup.  Put water in a tea pot, it becomes the tea pot.  Water can crash or water can flow.  Be water my friend. 


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InvisibleNimpo
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Re: -B plus' agar work thread- [Re: jjhitman]
    #17999849 - 03/23/13 03:45 PM (11 years, 1 month ago)

:popcorn:

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Offlinejjhitman
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Re: -B plus' agar work thread- [Re: Nimpo]
    #17999921 - 03/23/13 04:06 PM (11 years, 1 month ago)

Not worth it.


--------------------
Put water in a cup, it becomes the cup.  Put water in a tea pot, it becomes the tea pot.  Water can crash or water can flow.  Be water my friend. 


Edited by jjhitman (03/23/13 08:46 PM)

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