gar.. dog food agar questions ( silly obstruction..) - Advanced Mycology

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vampirism
Stranger

Registered: 03/14/04
Posts: 8,120

gar.. dog food agar questions ( silly obstruction..)

#1795285 - 08/09/03 12:42 PM (21 years, 4 months ago)

Ok, i was planning on using rush wayne's peroxide method with dfa
but i have run into a very simple problem: i have no scale

does anyone know of an average density for dry agar agar?
ill get the volume of 20g dry dog food just by dividing total weight by volume and so forth.. but i dont know the weight of my agar at all

and also

using the peroxide method, how important is it to have sterile h2o2? Is there some way I could check the sterility? I have some mildly old peroxide, but is it ok to use?

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Sev
Astropath

Registered: 06/06/03
Posts: 1,426
Loc: NY
Last seen: 10 years, 1 month

Re: gar.. dog food agar questions ( silly obstruction..)

[Re: ]
#1795688 - 08/09/03 03:07 PM (21 years, 4 months ago)

You don't need sterile H2O2 -- after all, it's there to kill things. :wink:

So long as the peroxide was stored in a cool, dark place, it should be fine for a year or two.

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"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.

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vampirism
Stranger

Registered: 03/14/04
Posts: 8,120

Re: gar.. dog food agar questions ( silly obstruction..)

[Re: Sev]
#1795912 - 08/09/03 04:23 PM (21 years, 4 months ago)

aye, but i read that sometimes it has mold particulates floating around- sort of establish mold mycelium which would contam my plates

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micro
bunbun has a gungun

Registered: 05/09/03
Posts: 7,532
Loc: Brick City

Re: gar.. dog food agar questions ( silly obstruction..)

[Re: ]
#1797029 - 08/10/03 12:02 AM (21 years, 4 months ago)

Not much will live in hydrogen peroxide. The volume of the agar depends on the specific agar, but you could always do it by the amount of water displaced.... You'd have to know the specific density, however, but this may be on the bottle.

A drop scale should work, or a nickel weighs 5 grams. It's not really sensitive, but you want to be close.

BTW -- dog food agar contaminates really easily, IME. I don't use peroxide, however.

--
Micro

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vampirism
Stranger

Registered: 03/14/04
Posts: 8,120

Re: gar.. dog food agar questions ( silly obstruction..)

[Re: micro]
#1797674 - 08/10/03 08:45 AM (21 years, 4 months ago)

interesting.. i've noticed some places that recommend it, and have used it successfully for morels and other interesting fellas.. im going to use it on pl. djamor ( most likely), so i shouldn't run into contam problems, esp with peroxide

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micro
bunbun has a gungun

Registered: 05/09/03
Posts: 7,532
Loc: Brick City

Re: gar.. dog food agar questions ( silly obstruction..)

[Re: ]
#1798172 - 08/10/03 12:58 PM (21 years, 4 months ago)

Doubtful you'll have problems with peroxide. Like I said -- I don't use it, so I have to worry about what I use more.

I will use gentamycin sometimes, though.

--
Micro

--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Mushrooms, Mycology and Psychedelics > Advanced Mycology

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