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 zippythepinhead
Mindlessconsumer ofcommercialproducts
Registered: 06/17/03
Posts: 18
Last seen: 13 years, 9 months
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 Is this correct PF tek? 
#1797793 - 08/10/03 12:07 PM (14 years, 13 days ago) |
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If someone were to get contamination in a PF tek type system, and noticed that the BRF/verm came out as a clump when discarding it, would it be ok? It would start out as fluffy mixture, and clumpify after PC'ing? If other containers don't show contam then it's best to leave them alone and see what happens. I never read anything in PF tek about fluffing mixture after PC'ing. That would seem like a less than ideal thing to do.
-ZTPH
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Happiness is feeling zestfully clean.
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 dog
straw dog
Registered: 06/20/02
Posts: 2,790
Loc: Route 66
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If there is no contamination then let them be. How long has it been since you inoculated?
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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,105
Loc: London 
Last seen: 7 months, 13 days
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are we having fun yet?
yes, they will form a sort of block during the sterilisation, but you won't know this, as the jar will be full (top half inch dry verm). here is the best pftek variation i've seen:
www.fungifun.org/pf
sk 
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buh
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zippythepinhead
Mindlessconsumer ofcommercialproducts
Registered: 06/17/03
Posts: 18
Last seen: 13 years, 9 months
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Re: Is this correct PF tek? [Re: dog]
#1797840 - 08/10/03 12:26 PM (14 years, 13 days ago) |
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Wow, is this place fast, or what? Inocculation occurred 7 days ago of the last of the TC strain in syringe. All other non-PC'ed attempts, and spawn bag were failures due to ignorance at the time. Contamination ocurred at injection site. One other site opposite contam showed correct growth. The good site was transferred to another container in the hope of continuing it. Syringe was PC'ed, and reloaded with EC spores from TLG and hydrated with sterile, distilled water for 24 hr. Two other containers were inocculated 4 days ago. EC was suggested here for the newbie. TLG was suggested here as reputable. A spore print leaves no doubt as to what it is. Original syringe came with "jelly" blobs floating around in it, and was in doubt. Hand-holding requested until first microscopy investigation.
BTW: I never read this, but distilled water is excellent for the PC. Regular water around here (ground water) leaves PC with messy residue. Regular water for PF tek, though.
Thank you,
-ZTPH
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Happiness is feeling zestfully clean.
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