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OfflineEnneagram
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Two PF Tek Questions
    #6169642 - 10/14/06 06:02 PM (17 years, 5 months ago)

hiya, I've got two PF tek questions.


I happen to live right near a very nice river (it's closer to me than the grocery!)
I'd like to use this natural water for its nutrients, but mostly because I'd like to keep everything as cheap as possible (in the PF jars). I'm really, really cheap.

However, I have learned that you usually need a pressure cooker when using natural water, because it has a higher chance of contams. I do not have a pressure cooker, so I was wondering, what if I go collect the water, boil it, and boil it again? Will that kill the contaminations in the natural water? Will the temp be high enough at boiling?

If not, I guess I have to spend the lousy 2 bucks on poland Spring!


Second question I have..
I'm planning on the fractional sterilization technique because I don't have a pressure cooker. I'm wondering, when I'm done boiing the jars for the first time (fractional here means boiling the jars three times) and I let them sit for the 22 before I boil them again, where should I keep them?
I can't just let them sit in the pot with the lid on cause' other people need to use the pot, but I don't want to set the jars in a vulnerable area. Is okay if I just place them in the incubating chamber (without the heating element on obviously)? What do you think?

I appreciate you reading!


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Nothing new under the sun.

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Offlinerross
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Re: Two PF Tek Questions [Re: Enneagram]
    #6169667 - 10/14/06 06:12 PM (17 years, 5 months ago)

i would think that boiling would get rid of the contams....but you can get distilled water for under a buck. i would just buy it distilled. I would think also that placing them in the clean incubate would be fine. after all they go in there anyways after you boil them...... good luck


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"Prohibition... goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation and makes a crime out of things that are not crimes. A prohibition law strikes a blow at the very principles upon which our government was founded." -- Abraham Lincoln December, 1840

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OfflineCiv
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Re: Two PF Tek Questions [Re: Enneagram]
    #6169770 - 10/14/06 06:57 PM (17 years, 5 months ago)

Useing natural water... seems to have many unknown variables that you would add to your project- You can buy water thats made the same way, every time, and you can have more control of your experiment.

Quote:

I'm wondering, when I'm done boiing the jars for the first time (fractional here means boiling the jars three times) and I let them sit for the 22 before I boil them again, where should I keep them?
I can't just let them sit in the pot with the lid on cause' other people need to use the pot, but I don't want to set the jars in a vulnerable area. Is okay if I just place them in the incubating chamber




That should be fine since you HAVE to- Its on the final boil you will kill lots of contams that started growing after the first two. Always best to try and play the TEKs out 100%.


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"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar

Edited by Civ (10/14/06 06:58 PM)

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Offlineroyer
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Re: Two PF Tek Questions [Re: Civ]
    #6169939 - 10/14/06 08:02 PM (17 years, 5 months ago)

i just use tap water and it works fine and i didnt use to have a pc and all i did is boil on high in a pot w/lid. water 1 inch up the jars and washcloth on the bottom and tin foil covering the jars.

45 min no contam


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=================================================
if you have any questions please feel free to pm me , thx :-)

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OfflineQuake3
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Re: Two PF Tek Questions [Re: royer]
    #6170142 - 10/14/06 09:31 PM (17 years, 5 months ago)

I would assume tap water wouldn't have that many contams because it's heavily regulated (unlike bottled water) and has traces of chlorine in it.

With natural water you're probably taking a big risk. I would just go with distilled water. You're going to get contams and waste a month or two before either giving up completely or giving in and just purchasing a PC or at least distilled/bottled water and a few other things for a better 2nd try.

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OfflinePr0_X
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Re: Two PF Tek Questions [Re: Quake3]
    #6170453 - 10/14/06 11:24 PM (17 years, 5 months ago)

Tap water is fine to use, if you want to be extra cautious pre-boil it like you said.

Fractional sterilization is also a sure shot way to sterilize your substrate jars. Your on the right path my friend.


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It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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InvisibleOldSpice
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Re: Two PF Tek Questions [Re: Pr0_X]
    #6170555 - 10/15/06 12:00 AM (17 years, 5 months ago)

Quote:

Pr0_X said:
Tap water is fine to use, if you want to be extra cautious pre-boil it like you said.

Fractional sterilization is also a sure shot way to sterilize your substrate jars.



A pressure cooker will do it right the first time
Ebay


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So hard to be ....WDWGFH?
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Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


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OfflineEnneagram
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Re: Two PF Tek Questions [Re: OldSpice]
    #6172526 - 10/15/06 04:56 PM (17 years, 5 months ago)

Pressure cooker - nice. But gee, everyone is going to wonder why the hell I got a pressure cooker  :grin:

My tap water is bad...we had to shock our well, but I still don't trust it!

Tap water is fine to use, if you want to be extra cautious pre-boil it like you said.


So what if I boiled my river water? Would this get hot enough sanitize? I really just want to figure this out. I'm not opposed to buying water, I'm just really curious at this point.

Also, an off topic question, and I feel bad for asking, but I'll just throw this out. I was never good with arithmetic. I just purchased a 3 pound bag of brown rice flower. How do I figure out how many cups I can get out of 3 lbs of flower?! Should I go to a math forum with this? I need 3 cups for 12 PF half-pint jars. Thanks to anyone who can help me!

Appreciated greatly,
Enneagram

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Offlinehyphae
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Re: Two PF Tek Questions [Re: Enneagram]
    #6172859 - 10/15/06 06:11 PM (17 years, 5 months ago)

Boil any water that you are using to moisten your BRF don't take an unnecessary risk it is just foolish! When PCing it is unnecessary as PCing will kill everything. You only need to rapidly boil the water for 5min to kill contams in water (fact).


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineEnneagram
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Re: Two PF Tek Questions [Re: hyphae]
    #6173479 - 10/15/06 09:38 PM (17 years, 5 months ago)

Thanks for the information, guys!

I think I figured out how many cups come out of a 3 lbs bag of BRF - it's 6 cups.


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Invisiblejeetered
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Re: Two PF Tek Questions [Re: Enneagram]
    #6173498 - 10/15/06 09:44 PM (17 years, 5 months ago)

i dont see why having the incubation chamber warm would be a problem in between traditional boilings, if anything WAS there, it would "hatch", then boiling them again would be sure to kill it.

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OfflineEnneagram
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Re: Two PF Tek Questions [Re: jeetered]
    #6173539 - 10/15/06 09:55 PM (17 years, 5 months ago)

That's actually a good point. This seems to be contrary to another tyndallization technique I've read, but a shroomery.org article confirms what you are saying.

Thanks a lot.

Enneagram


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Nothing new under the sun.

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Invisiblejeetered
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Re: Two PF Tek Questions [Re: Enneagram]
    #6174644 - 10/16/06 10:16 AM (17 years, 5 months ago)

much like an endore spore soak for grains, fits the same general concept.

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OfflineAcero
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Registered: 03/17/13
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Last seen: 10 years, 11 months
Re: Two PF Tek Questions [Re: jeetered]
    #17968319 - 03/17/13 11:46 AM (11 years, 4 days ago)

Is sterilizing really necessary?


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--Shrooms--

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OfflineAcero
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Registered: 03/17/13
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Re: Two PF Tek Questions [Re: jeetered]
    #17968323 - 03/17/13 11:47 AM (11 years, 4 days ago)

I'm following this PF TEK: http://www.shroohttp://www.shroomery.org/10256/PF-Tek-v2mery.org/10256/PF-Tek-v2
Interesting fact PF stands for the guy who created the PF jar and TEK.

Where is a spore solution available, and how much is needed in a 1/2 pint jar?
Is there anything needed to make the spores germinate?

If the mushrooms are not dried do they lose their potency? If so after how long? If the mushrooms are dried in the open air do they lose their potency?

When spore printing, how do you get the spores out of the cap?

Edited by Acero (03/17/13 12:08 PM)

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