|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Genetically modify yeast to produce psilocin/psilocybin 1
#17956628 - 03/14/13 06:46 PM (11 years, 2 months ago) |
|
|
Its completely reasonable that a plasmid auto replicating sequence could be isoloated from active mushrooms. This sequence could in turn be give to yeast, who will then assemble the sequences into their own.
Apparently fungi are the easiest lifeform to engineer.
The implications are mind numbing. Brew a pail of sugar/nutrients for a week or 2 etc...
Edited by Sillyputty67 (03/14/13 06:47 PM)
|
Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 7 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#17956735 - 03/14/13 07:02 PM (11 years, 2 months ago) |
|
|
-------------------- Β―\_(γ)_/Β―
|
Amphibolos
Le bourgeois gentilhomme




Registered: 05/22/09
Posts: 626
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Tmethyl] 2
#17957398 - 03/14/13 09:06 PM (11 years, 2 months ago) |
|
|
I think the problem atm is that the genome of the psilocybe species arent sequenced.
Also it is hard to implement the psilocybin pathway if you dont know the enzymes implicated in the process itself. Also, to get a suitable yield, you would have to find the limiting steps in the pathway and find a way to bypass its limitations.
However, i think that sequencing the genome would cost us like what 500$? And i'm sure that some enthusiasts (myself included) wouldnt mind working on the sequence. I dont have experience regarding to genome annoting but they are developping alot of tools that make it somewhat easy.
It could be made in a kickstarter form, its a nice project and im sure alot of people would enjoy it 
It could be possible to do the same thing for the THC biosynthesis pathway since im sure they already managed it for some opiates
Here is a metabolic pathway of another basidiomycete (Coprinus cinerea), it give you an idea of the work that needs to be done. Coprinus cinerea
--------------------
"Homo sum ; humani nihil a me alienum puto"
Edited by Amphibolos (03/14/13 09:21 PM)
|
Sexycybe
bio-nut


Registered: 05/03/11
Posts: 518
Last seen: 1 year, 8 days
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos]
#17959150 - 03/15/13 05:57 AM (11 years, 2 months ago) |
|
|
Saw a post on this 2 years ago but instead of psilocybin they proposed THC. they only estimated it to be around $40,000-$60,000 from memory..
-------------------- Successful indoor psilocybe subaeruginosa grow Nothing written above is real; it is merely a fabricated story of nonsense. Nothing said is to be believed in any way or taken seriously in the slightest.
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sexycybe]
#17959192 - 03/15/13 06:27 AM (11 years, 2 months ago) |
|
|
Wow,very nice Guys!!!!! I saw some kits for around 600$ on a science site a few months back, for genetic engineering, and biotech. I dont have the foggiest idea where to begin isolating a sequence. Perhaps if we all put our heads together, pooled some money, we could make this happen. 
edit. I think a huge difference between thc and psilocybin, is psilocybin is water soluble, making it much easier to deal with post production.
-------------------- 1) Everything I ever posted or say is a lie.
Edited by Sillyputty67 (03/15/13 06:34 AM)
|
Sexycybe
bio-nut


Registered: 05/03/11
Posts: 518
Last seen: 1 year, 8 days
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#17962813 - 03/15/13 11:59 PM (11 years, 2 months ago) |
|
|
Yeah it was said the oil would have to be skimmed daily, more demand for it as apposed to psilocybin too. seeing as it is legal in some areas and used for medicine, it would be possible to get a research grant so they don't have to bother growing marijuana and then refine the oil.
-------------------- Successful indoor psilocybe subaeruginosa grow Nothing written above is real; it is merely a fabricated story of nonsense. Nothing said is to be believed in any way or taken seriously in the slightest.
|
nooneman


Registered: 04/24/09
Posts: 14,700
Loc: Utah
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#17962930 - 03/16/13 12:54 AM (11 years, 2 months ago) |
|
|
First, this really isn't advanced mycology.
Maybe it would be possible for a team of people with PhDs and millions of dollars of grant money and access to high end professional labs to attempt something like this, but for all practical purposes what you're proposing is totally impossible.
It's also totally useless. Isn't not like cubes are hard to grow. Psilocybin is already insanely easy to make in the form of psilocybin producing mushrooms. There would be no benefit to making psilocybin producing yeast.
|
cli_hlt
Beshroomed



Registered: 10/29/11
Posts: 462
Loc: EU
Last seen: 4 months, 27 days
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: nooneman]
#17963512 - 03/16/13 06:53 AM (11 years, 2 months ago) |
|
|
Maybe there's an escape route.
I heard that the (almost) spent substrate - if it's edible like rice - can make you trip is you eat it.
Maybe someone can brew an LC and extract the (possible) psilocybin content.
Any thoughts?
|
Sexycybe
bio-nut


Registered: 05/03/11
Posts: 518
Last seen: 1 year, 8 days
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: cli_hlt]
#17963534 - 03/16/13 07:03 AM (11 years, 2 months ago) |
|
|
why not just eat the mushrooms and drink a beer? There is not much point in making an alcoholic LC solution... plus ethanol tends to kill mycelium
The idea here is that instead of the yeast producing ethanol as a waste it produces psilocybin but as nooneman stated, may as well just grow the mushrooms, it's not hard
-------------------- Successful indoor psilocybe subaeruginosa grow Nothing written above is real; it is merely a fabricated story of nonsense. Nothing said is to be believed in any way or taken seriously in the slightest.
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67] 1
#17963628 - 03/16/13 07:57 AM (11 years, 2 months ago) |
|
|
Quote:
malicom said: Its completely reasonable that a plasmid auto replicating sequence could be isoloated from active mushrooms. This sequence could in turn be give to yeast, who will then assemble the sequences into their own.
If it's completely reasonable then do it and tell us how it went. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
cli_hlt
Beshroomed



Registered: 10/29/11
Posts: 462
Loc: EU
Last seen: 4 months, 27 days
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sexycybe]
#17963640 - 03/16/13 08:03 AM (11 years, 2 months ago) |
|
|
I phrased it bad sorry. I did not mean alcoholic fermentation of course.
I tried to say that by making a thick LC you can get active compounds quickly. Quicker than growing the actual mushrooms. It would be interesting to see how potent an LC could be.
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: RogerRabbit]
#17964096 - 03/16/13 11:33 AM (11 years, 2 months ago) |
|
|
Quote:
RogerRabbit said:
Quote:
malicom said: Its completely reasonable that a plasmid auto replicating sequence could be isoloated from active mushrooms. This sequence could in turn be give to yeast, who will then assemble the sequences into their own.
If it's completely reasonable then do it and tell us how it went. RR
RR your a smart fucker with a lot of experience, but your angles here are often times hostile, or ultra condescending. If anyone were to give some good input i would hope it were you. Instead you hint that im on my own, and this is not a place to share ideas, only methods.
Yeast are fungus. Hence mycology.
An invention always starts as an idea. I assumed the place for input on this idea was here, and a place like this could make this kind of idea a reality. I guess not. I guess this is only a place for methods already accomplished, and not bleeding edge science. I guess im the ass for assuming....
-------------------- 1) Everything I ever posted or say is a lie.
Edited by Sillyputty67 (03/16/13 12:34 PM)
|
cli_hlt
Beshroomed



Registered: 10/29/11
Posts: 462
Loc: EU
Last seen: 4 months, 27 days
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#17964179 - 03/16/13 12:03 PM (11 years, 2 months ago) |
|
|
To be more on-topic and hopefully release some pressure from above this topic I'd like to give you a few pieces of information I've recently acquired during my adventures in hobby-biotech (yepp, there IS such thing).
There is a worldwide movement called DIYBio.
TED talk: http://www.ted.com/talks/ellen_jorgensen_biohacking_you_can_do_it_too.html
There's also a google group.
Those gals and guys are doing some pretty amazing stuff.
The $600 kit you've talked about is probably the OpenPCR wich is a polymerase chain reaction thermocycler. It does not sequences the DNA, but can produce a lot of DNA from very little. Can be used to multiply DNA or certain DNA pieces (like a specific gene).
The result can be used to draw scientificly-looking colored bars wich can be compared to see if the neighbour's dog pissing on your plants, or can be put into a sequencing machine.
Home biotech is bit like home computers were in the mid-late 75's or early 80's. No ordinary people could acquire them and use them.
But similarly to early computers biotech is growing and evolving very rapidly. This is the next big thing. The price of full DNA sequencing fell down from millions on dollars to thousands in maybe ten years.
There are free, open source software packages (like gentle, http://gentle.magnusmanske.de/) that can be used to edit/design DNA, protein analysis/secondary structure prediction, and a lot more.
Good luck with your plans
Edited by cli_hlt (03/16/13 02:09 PM)
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: cli_hlt]
#17964188 - 03/16/13 12:06 PM (11 years, 2 months ago) |
|
|
Very nice Sir. 
Now thats what im talking about!!
Perhaps something like this could be on the horizon for ordinary folk.
-------------------- 1) Everything I ever posted or say is a lie.
|
Mr.Al
Alphabet soup


Registered: 05/27/07
Posts: 5,388
Loc: N.S.A. D.C.
Last seen: 4 months, 18 days
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#17964302 - 03/16/13 12:37 PM (11 years, 2 months ago) |
|
|
It would make an eye-catching headline if something like that happened.
Mycology works best the cleaner everything is.
Beyond that increase efficiencies of work, methodological work, work that improves your economy of motion and concentrated Awareness in the Laboratory; that will accelerate Productive Capacity.
Get rid off EVERYTHING extraneous in the WOrk Room. No artwork, Nothing on the Floors. Store extra materials in a separate clean room that is for storage of Professional materials only.
Take a THOROUGH SHOWER before entering room, WOrk room Clothes only.
DO not work when out of FOCUSED CONCENTRATION is GOOD ADVICE:
Avoiding COntamination of LAB is PAramount Indeed.
[i]Working Drunk or EVEN SLIGHTLY intoxicated could possibly, It has Before, wipe out months of work.
If you are Truly Patient you will show Discipline and become Professional in your Avocations and Hobbies.
WHy not check out HErmes' poll in Golden Dawn Thread you Fungi Noshing Fuckers...If you'd like, or are you fright of Bamboozles or Confounmnents?
|
PsilyPhily
Stranger


Registered: 02/07/13
Posts: 560
Last seen: 10 years, 7 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Mr.Al]
#17973241 - 03/18/13 09:38 AM (11 years, 2 months ago) |
|
|
Not trying to be condescending but wouldn't it be easier and cheaper to make pure psilocybin with a child's chemistry set?
I'l admit I loved the idea its just that with a single chemist's help we could make pure chemical with easy to get hold of elements, Probably alot easier than transforming yeast using genetic modification..
Phil.
-------------------- "If a drug (or technique or process) were ever to be discovered which would consistently produce a plus four experience in all human beings, it is conceivable that it would signal the ultimate evolution, and perhaps the end, of the human experiment." β Alexander Shulgin, PiHKAL, 1991 "Well Shulgin, you clearly haven't tried salvia 150X extract, good effort though."
Phil.
|
Amphibolos
Le bourgeois gentilhomme




Registered: 05/22/09
Posts: 626
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: nooneman] 1
#17978501 - 03/19/13 09:10 AM (11 years, 2 months ago) |
|
|
Quote:
nooneman said: First, this really isn't advanced mycology.
Maybe it would be possible for a team of people with PhDs and millions of dollars of grant money and access to high end professional labs to attempt something like this, but for all practical purposes what you're proposing is totally impossible.
It's also totally useless. Isn't not like cubes are hard to grow. Psilocybin is already insanely easy to make in the form of psilocybin producing mushrooms. There would be no benefit to making psilocybin producing yeast.
I think you are wrong for most of what you wrote,
Since when mycology is only about cultivating techniques? Mycology is about the study of the Biology of fungi and Saccharomyces cerevisiae is still classified as an ascomycete therefore it is a fungus.
You have to see the project that OP has mentionned in several steps, it wont and cant be done in an instant but some of it could be done with basic biological knowledge. As someone pointed out, there are alot of programs that are open source that could permit us to manage the sequencing of the genome of P. cubensis. Once that is done we could work on annoting the genome based on the similitude of already sequenced fungi (like Coprinopsis cinerea).
I thought of it, and i agree that producing psilocybin in yeast wouldnt be that useful since the cubes are already easy to grow and spread. However, having a better understanding of P.cubensis genomic structure could make us able to produce strains with desired traits with higher efficiency and high success rates.
However, i dont think we could go that far without a proper lab, but the genome sequencing and annoting is done in silico so that could be done with the help of motivated members.
I checked at my university, we dont have access to the illumina sequencing technology, so we have to find a place where they have those installations. I contacted one of my friend relating to cost of sequencing a 36 to 50mb genome. Ill post it when its done.
--------------------
"Homo sum ; humani nihil a me alienum puto"
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos]
#17979325 - 03/19/13 01:01 PM (11 years, 2 months ago) |
|
|
The benefit of yeast producing active compounds is for industrial scale production. You can scale yeast fermentation way beyond mushroom cultivation much simpler and cheaper. Not to say I per se need an industrial producer. But I wouldnt mind.... You could literally reduce a 30 hour process to 2, and if thats not a good enough reason, im not sure what is..
When i make moonshine. It takes 30 min to prepare the mash, and add the yeast. It takes 2 weeks of unattended time. Unlike a fruiting chamber. It takes 3 hours to distill the alcohol. Granted you would need to find another extraction process instead of distillation. Perhaps just evaporation. If you had a product that was 1% active compounds, You could simply evap to a powder and press into tabs. The leftover metabolites of the yeast are healthy, and would make a good buffer.
The other thing to keep in mind here, is if any of us can source a machine to sequence, we need to be able to get a sample of p. Cubensis to the machine without getting incarcerated.
Edited by Sillyputty67 (03/19/13 01:34 PM)
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#17988333 - 03/21/13 07:09 AM (11 years, 1 month ago) |
|
|
Quote:
malicom said:
Quote:
RogerRabbit said:
Quote:
malicom said: Its completely reasonable that a plasmid auto replicating sequence could be isoloated from active mushrooms. This sequence could in turn be give to yeast, who will then assemble the sequences into their own.
If it's completely reasonable then do it and tell us how it went. RR
RR your a smart fucker with a lot of experience, but your angles here are often times hostile, or ultra condescending. If anyone were to give some good input i would hope it were you. Instead you hint that im on my own, and this is not a place to share ideas, only methods.
Yeast are fungus. Hence mycology.
Wow.
I was hoping to gently prod you into discovering/learning the difference between a single celled organism and mushroom mycelium where the alkaloids are produced. Us science-type people like to classify things. However, yeast doesn't really care if we call it 'fungi' because of its reproductive method. My point was to get you to look closer at what you proposed by investigating rather than sitting in front of the computer waiting for someone to tell you. I apologize for asking too much. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 21 hours
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: RogerRabbit]
#17988563 - 03/21/13 09:00 AM (11 years, 1 month ago) |
|
|
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: RogerRabbit]
#17988569 - 03/21/13 09:03 AM (11 years, 1 month ago) |
|
|
Ive been doing some research. Granted im not top of my game where this is concerned. Now this is a more reasonable answer. There's why im asking in this forum. I just started to study fungus late last year. This rabbit hole is sooooo deep. Itll take some time.... Mind you im no university student etc.. Just a normal smart ass type, who dosnt believe in imposibillity. I have a very good understanding of science. Biology is kinda a weak point tho, as I didnt find interest when i was a child. I always was working the electrical/technical/physics/astronomy angles. I also have an excellent grasp of inorganic chemistry, and have some elementary skill in organic chem as well. When I got into brewing and Marijuana cultivation, and aquariums, my interest in biology peaked, so here I am. I am a network engineer by trade, and make the impossible happen every day.
I read a lot of science news, and it seems that genetic engineering is getting simpler. Almost simple enough for me to start tackling it.
Use a machine. Sequence an active, and nonactive. Use the computer to compare rna pairs and point out which are present in the active, and not in the nonactive, and then start looking through the remaining metabolic processes, and establish a process of elimination.
Then get the right enzymes to break off, and isolate the exact sequence you need.
This is where my understanding wanes. You give it to yeast and they assemble it automatically. Magically..... Yes more research will be required on my part, but in no way did I say that i wasnt geared to do any... You just liberally lump me into a category with the other lazy brains, and treat me accordingly.
I saw a Ted Talk last year where a brilliant professor re-wrote the dna of an E-coli, and watermarked it with a special code. Methylated the cell, lo and behold it "booted up" and became the worlds first synthetic life. Yeast can apparently assemble dna of any genus or species. Im not sure what gives yeast these seemingly magical properties.
Ive also had previous ideas of isolating the FoxP2 gene, which is only present in humans. It is considered responsible for speech and higher thought processes in humans. If this could somehow be given to a dog, well, Ill let you imagine that scenario.
When I brought this idea here, It was because i respect this community, and value its input. I am a science-type. I understand your point of classification. Yeast is fungus,and the argument was whether or not this fell under an "advanced mycology topic", and in no way did i misclassify. You just circumlocuted by saying yeast dosnt care what you call it. Thats a cop out. This was the first step in trying to make this a reality. It would be a good project for beginning simple engineering. In 10 years kids will be doing this in middle school.
http://blogs.smithsonianmag.com/artscience/2013/02/the-story-of-how-an-artist-created-a-strange-genetic-hybrid-of-himself-and-a-petunia/

RR your posts are often of a "tag em, bag em, ship em out" mentality. Your angle here is askew. If you read my posts, im trying to get a platform of individuals to contribute to this. Pool money, brain power etc. Because you are the man, your rants, flames, and unconstructive contributions are tolerated. You abuse this position. I think you would be hard pressed finding someone to disagree with this deduction.
If this idea didnt give you a "giggity giggity, i want to contribute to this project." notion and instead gave you a "I want to defer, shut down, and discourage." kind of answer, there is something wrong with your wiring..... This behavior is often referred to as troll like.

RR I can however thank you for your positive contributions in other posts, regardless if your the mega troll for some reason in mine. Lols. Especially when the next comparable post in "advanced mycology" is "PC petri dishes?"......... 
Frankly i dont give a shit how prestigious a diploma you hoarde and masturbate, or how you look to yourself in the mirror everyday. I did not mean to injure your ego, by posting something out of your ballpark. I get folk with masters degrees around me all the time, and dont have half of the skill or tricks that I have. I work with doctors, and high powered admin staff, who look up to and respect me. I respect others intellects by how they come off, how intuitive they are, how logical their process is, and frankly just how easy to get along with they are. A piece of paper means shit to me. I dont care about the (overpriced society stamp of approval) that certifies that you can absorb, regurgitate, and forget some data. Ill work with anyone who shows initiative, and skill, regardless of the amount of facts the person has absorbed. In todays world of technology, all these processes are relatively super simplified, by the vast processing power of computers, which is my forte. Ive built my own linux distro's from scratch (gentoo stage 1 many times), so complexity is not a stumbling point. Ive built openmosix and beowulf supercomputing platforms. I work with a virtualization environment everyday. So condescention is just an indication of some sort of ego complex. RR just be cool bro. We all have our specialties. Yes, I too have an ego. One big enough to not be intimidated by yours. We are all people here, and have a common goal. To change the world in a positive light with science. So hopefully that is something we can agree upon, and is an idea you can get behind. 
Check this link, its the ted talk i saw a while ago. It has been my inspiration, and has unlocked a tenacity.  http://www.ted.com/talks/craig_venter_unveils_synthetic_life.html
Edited by Sillyputty67 (03/21/13 07:24 PM)
|
Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 19 hours
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: cli_hlt]
#18004285 - 03/24/13 03:12 PM (11 years, 1 month ago) |
|
|
Quote:
cli_hlt said: Home biotech is bit like home computers were in the mid-late 75's or early 80's. No ordinary people could acquire them and use them.
There is a biotech hacker space here in silicon valley, and another is starting in oakland.
http://biocurious.org
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Alan Rockefeller]
#18004291 - 03/24/13 03:14 PM (11 years, 1 month ago) |
|
|
Alan, I read your bio a while ago. Have you sequenced active species already?
-------------------- 1) Everything I ever posted or say is a lie.
|
Amphibolos
Le bourgeois gentilhomme




Registered: 05/22/09
Posts: 626
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#18004316 - 03/24/13 03:20 PM (11 years, 1 month ago) |
|
|
Also, you have to ask he knows or manipulated a platform using at least the illumina technology for nuclear genome sequencing.
We have one at McGill university, but we will need DNA of intense purity
--------------------
"Homo sum ; humani nihil a me alienum puto"
Edited by Amphibolos (03/24/13 03:21 PM)
|
Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 19 hours
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67] 1
#18004319 - 03/24/13 03:21 PM (11 years, 1 month ago) |
|
|
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Alan Rockefeller]
#18004330 - 03/24/13 03:23 PM (11 years, 1 month ago) |
|
|
-------------------- 1) Everything I ever posted or say is a lie.
|
Amphibolos
Le bourgeois gentilhomme




Registered: 05/22/09
Posts: 626
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Alan Rockefeller]
#18004341 - 03/24/13 03:25 PM (11 years, 1 month ago) |
|
|
Its the parts of the genome used for taxonomy.
What we would need is a complete nuclear genome sequencing
Do you have a platform using the illumina sequencing at your university?
--------------------
"Homo sum ; humani nihil a me alienum puto"
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos]
#18004353 - 03/24/13 03:27 PM (11 years, 1 month ago) |
|
|
This. He just gave it to us.
1 gatcattatt gaataacttt ggcgtggttg tagctggtcc tctcgggggc atgtgctcgc 61 ccgtcatctt tatatctcca cctgtgcacc ttttgtagac gttggaactg gataggagag 121 ggacttgtcc ttcaagttga aggttttttc ggcgctctac gttttcatat accccaaaga 181 atgtaacaga atgtatctta tggctttatg cctataaact atatacaact ttcagcaacg 241 gatctcttgg ctctcgcatc gatgaagaac gcagcgaaat gcgataagta atgtgaattg 301 cagaattcag tgaatcatcg aatctttgaa cgcaccttgc gctccttggt attccgagga 361 gcatgcctgt ttgagtgtca ttaaattctc aaccttacca gcttttgtta gcttgtgtaa 421 tggcttggac ttgggggtat tttgccggct tctcttgaga tgtcagctcc ccttaaatgc 481 attagccggc tgcccgctgt ggaccgtcta ttggtgtgat aattatctac gccgtggacg 541 tctgctctca atgggttgaa gctgcttcta accgtccgtt tattcggaca gcacataatg 601 acaatttgac ctcaaatcag gtaggactac ccgctgaact taagcatatc aataagcgga 661 ggaaaagaaa ctaacaagga ttcccctagt aactgcgagt gaagcgggaa aagctcaaat 721 ttaaaatctg gcggtctttg gctgtccgag ttgtaatcta gagaagtgtt atcc
adenine, cystosine, cant remember....... I was using a protein folding game called eterna. It is exclusively about solving the riddles of how the acids bond in stable structures. Its actually pretty sweet.
-------------------- 1) Everything I ever posted or say is a lie.
Edited by Sillyputty67 (03/24/13 03:30 PM)
|
Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 19 hours
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos]
#18004374 - 03/24/13 03:31 PM (11 years, 1 month ago) |
|
|
Quote:
Amphibolos said: Its the parts of the genome used for taxonomy.
Yes - Mushrooms have around 40 million base pairs and I chop out just 1000 or so, usually with ITS primers.
Quote:
What we would need is a complete nuclear genome sequencing
That's really expensive.
Quote:
Do you have a platform using the illumina sequencing at your university?
No I use sanger sequencing.
|
Amphibolos
Le bourgeois gentilhomme




Registered: 05/22/09
Posts: 626
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos]
#18004437 - 03/24/13 03:46 PM (11 years, 1 month ago) |
|
|
I just wanted to have an overview of how much it would cost.
Also, you'd have to pay them to make the cDNA library which is another thing
--------------------
"Homo sum ; humani nihil a me alienum puto"
|
Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 19 hours
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Amphibolos] 1
#18004471 - 03/24/13 03:52 PM (11 years, 1 month ago) |
|
|
Quote:
Amphibolos said: I just wanted to have an overview of how much it would cost.
The cost is highly variable at the moment.
I saw this on wikipedia:
Quote:
In June 2009, Illumina announced that they were launching their own Personal Full Genome Sequencing Service at a depth of 30Γ for $48,000 per genome.[58] This is still expensive for widespread consumer use, but the price may decrease substantially over the next few years as they realize economies of scale and given the competition with other companies such as Complete Genomics.[59][60] Jay Flatley, Illumina's President and CEO, stated that "during the next five years, perhaps markedly sooner," the price point for full genome sequencing will fall from $48,000 to under $1,000.[61] Illumina has already signed agreements to supply full genome sequencing services to multiple direct-to-consumer personal genomics companies.
I think the price is still closer to 48,000 than 1000, and I think a lot of places would charge a lot more than 48,000.
The lab I sometimes work in pays $10 per sample in chemicals alone to get 1000 base pairs. We run 96 samples at a time, so it's not a particularly cheap thing to do. Sometimes we get distracted talking about mushrooms and mess up, wasting the expensive chems.
|
teknix
πβπ
’ππ
π°π‘ πΌπ⨻



Registered: 09/16/08
Posts: 11,953
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Alan Rockefeller]
#18004573 - 03/24/13 04:15 PM (11 years, 1 month ago) |
|
|
Alan,
Do you work alongside a Bioinformaticist?
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 days, 21 hours
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#18004621 - 03/24/13 04:22 PM (11 years, 1 month ago) |
|
|
Quote:
malicom said:
1 gatcattatt gaataacttt ggcgtggttg tagctggtcc tctcgggggc atgtgctcgc 61 ccgtcatctt tatatctcca cctgtgcacc ttttgtagac gttggaactg gataggagag 121 ggacttgtcc ttcaagttga aggttttttc ggcgctctac gttttcatat accccaaaga 181 atgtaacaga atgtatctta tggctttatg cctataaact atatacaact ttcagcaacg 241 gatctcttgg ctctcgcatc gatgaagaac gcagcgaaat gcgataagta atgtgaattg 301 cagaattcag tgaatcatcg aatctttgaa cgcaccttgc gctccttggt attccgagga 361 gcatgcctgt ttgagtgtca ttaaattctc aaccttacca gcttttgtta gcttgtgtaa 421 tggcttggac ttgggggtat tttgccggct tctcttgaga tgtcagctcc ccttaaatgc 481 attagccggc tgcccgctgt ggaccgtcta ttggtgtgat aattatctac gccgtggacg 541 tctgctctca atgggttgaa gctgcttcta accgtccgtt tattcggaca gcacataatg 601 acaatttgac ctcaaatcag gtaggactac ccgctgaact taagcatatc aataagcgga 661 ggaaaagaaa ctaacaagga ttcccctagt aactgcgagt gaagcgggaa aagctcaaat 721 ttaaaatctg gcggtctttg gctgtccgag ttgtaatcta gagaagtgtt atcc
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
teknix
πβπ
’ππ
π°π‘ πΌπ⨻



Registered: 09/16/08
Posts: 11,953
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: PussyFart]
#18004651 - 03/24/13 04:28 PM (11 years, 1 month ago) |
|
|
guanine adenine thyamine cytosine, and uracil in rna.
|
Seahawk1
Stranger
Registered: 04/12/12
Posts: 139
Last seen: 8 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#18004817 - 03/24/13 04:57 PM (11 years, 1 month ago) |
|
|
Quote:
malicom said:
Quote:
RogerRabbit said:
Quote:
malicom said: Its completely reasonable that a plasmid auto replicating sequence could be isoloated from active mushrooms. This sequence could in turn be give to yeast, who will then assemble the sequences into their own.
If it's completely reasonable then do it and tell us how it went. RR
RR your a smart fucker with a lot of experience, but your angles here are often times hostile, or ultra condescending. If anyone were to give some good input i would hope it were you. Instead you hint that im on my own, and this is not a place to share ideas, only methods.
Yeast are fungus. Hence mycology.
An invention always starts as an idea. I assumed the place for input on this idea was here, and a place like this could make this kind of idea a reality. I guess not. I guess this is only a place for methods already accomplished, and not bleeding edge science. I guess im the ass for assuming....
Point is, people that know how "easy" this would be have already thought about...it's not like it's a novel idea. If we were all in a room together his tone would be less condescending and more joking... A. you can't just plop the psilo gene in a yeast and get psilo. Is it even a single gene? etc.. B. Like s/he said, it could be construed as a lead in for someone to do their own research, many of us have done this and thus, not had to make a post. C. Some of this stuff -IS- easy, just expensive. It's hard to convince people to give you $100k(or more) to maybe be able to make an illegal substance from yeast. What would an increasing [psilo] do to the culture?
I like talking about stuff too; but something like this isn't just a web forum brainstorm project. In chemistry elegance is key, growing shrooms and extracting is probably more elegant than a yeast method would be. JMO
|
Sillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 8 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Seahawk1]
#18004852 - 03/24/13 05:03 PM (11 years, 1 month ago) |
|
|
fair enough. I just say we make it so big pharma dosnt get it first.
Quote:
Seahawk1 said: What would an increasing [psilo] do to the culture?
Make them trip the fuck out or turn them blue. lolz
Blue yeast would change the world. There would no longer be a need for 25i or other synthetics. Other caveats would be what if this yeast escaped? Next thing you know all fruit along the eastern seaboard is now mildly psychedelic. gigity gigity.... Imagine the most pristine summer day, eating a fresh apple with a bluish hue. lol
-------------------- 1) Everything I ever posted or say is a lie.
Edited by Sillyputty67 (03/24/13 05:22 PM)
|
RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
|
Re: Genetically modify yeast to produce psilocin/psilocybin [Re: Sillyputty67]
#18005531 - 03/24/13 05:03 PM (11 years, 1 month ago) |
|
|
This thread has been closed.
Reason: This is advanced mycology, not a drug manufacturing forum. May I suggest ODD or PE.
|
|